Abstract The aim of this experiment was to determine the effects of housing nulliparous cows with non-lactating multiparous cows during the pre-calving period, on welfare and production-related parameters during the post calving period. Twenty nulliparous Holstein Friesian dairy cattle were assigned to one of two treatments (‘Mixed and Unmixed’) pre-calving. The ‘Mixed’ treatment involved housing experimental animals with non-lactating multiparous cows for three weeks prior to their expected calving date. In the ‘Unmixed’ treatment, experimental animals were housed with other nulliparous animals during the 3-wk period prior to their expected calving date. During this pre-calving period animals in both treatments were housed in groups of 10 animals, with the ‘Mixed’ group comprising of seven multiparous non-lactating cows and three experimental primiparous cows. The experimental animals were added to a resident group that contained 15 animals (10 multiparous cows and 5 primiparous cows) within 24 h of calving. The behaviour of the experimental animals was assessed immediately after the mixing period and also after feeding during the first month after introduction to the resident group (days 2, 4, 10, and 1 day in wks 3 and 4). In addition, data loggers were attached to the animals for a 24 h period on five occasions during the first month after calving to assess lying behaviour. Lying location and synchronicity of lying with the rest of the group was assessed by direct observation on two consecutive days each wk during the 2-h period prior to evening milking. Milk production, serum cortisol levels and changes in body condition and live weight were assessed during the first month after calving. ‘Mixed’ animals received fewer butts ( P P P P P P P
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