Attention to feeding practices is important because inadequate knowledge and inappropriate neonatal feeding are primary factors of neonatal morbidity. There is limited literature on the title and most of the results are focused on exclusive breastfeeding practices. Thus, this study aimed to assess Colostrum feeding practice and its associated factors among postnatal mothers who attend at health facility, Ethiopia.Institutional based cross-sectional study was conducted among postnatal mothers in Antsokia Gemza woreda health centers in North Shao zone, Ethiopia from January to March 2022. Systematic sampling technique was used to select 391 participants. Data were collected using a pre-tested interviewer-administered questionnaire. Data entry performed by using Epi data version 3.1 and was exported to SPSS version 25 for analysis. Descriptive statistics was done. Multivariable Logistic regression was used to predict the role of independent variables on colostrum feeding practice.The overall proportion of mothers feed colostrum to their neonate within an hour to five days which was low (64%.) when it is compared to WHO recommendation. WHO recommends that every newborn baby has to feed colostrum breast milk within 1 h after birth. Based on the finding from current study, it is better to provide culturally tailored health education for pregnant mothers by health extension workers focusing on the essentials of colostrum feeding for the betterment of their babies.