Chicken Feed Research Articles

Growth performance and nutritional profile of Tenebrio molitor raised on a diet composed of livestock feedstuff

AbstractEdible insects such as the mealworm (Tenebrio molitor) are emerging as an alternative animal protein resource to meet the needs of a growing global population. However, despite the successful mass production of edible insects, research into practical compound feed for edible insect is still insufficient. In this study, mealworms were fed wheat bran, layer chicken feed mash, chicken pellets and cattle pellets for 1 to 36 days, after which their body length was measured. In the groups fed with chicken feed mash, chicken pellets or cattle pellets, the mean body lengths were 2.88, 2.92 and 2.95 cm, respectively, similar to the 2.95 cm of the control T. molitor fed with wheat bran. Individual weight measurements yielded similar results: control, 7.87 g; chicken feed mash, 7.43 g; chicken pellets, 7.60 g; and cattle pellets, 7.63 g. Analysis of livestock feed intake showed that the intake was 290.4 g in control insects, 137.7 g in insects fed chicken feed mash, 220.2 g in insects fed chicken pellets and 230.3 g in insects fed cattle pellets. In the excretion analysis, the excretion of the control insects (161.60 g) was larger than that of the insects fed with chicken feed mash (93.37 g), chicken pellets (93.40 g) or cattle pellets (133.87 g). An analysis of the nutritional content of T. molitor based on nine major nutrients showed no significant differences between the control and the test groups. These results can be used to improve the price competitiveness and quality of T. molitor for commercial development.

Calcium, phosphorus, and phytic acid of fermented rice bran

Abstract Rice bran is one of the ingredients for poultry feed and is usually used in up to 10% of chicken feed. The restricted utilization of rice bran stems from its elevated levels of crude fiber (24.43%) and phytic acid (6.9%). Fermentation offers a means to diminish the amounts of crude fiber and phytic acid present. The fermentation requires carbon as an energy source for microorganisms. Palmyra sap (PS) can be used as a carbon source. PS is a local resource in East Nusa Tenggara that can be used as an additive. The objective of this study is to investigate the impact of fermentation duration, along with the addition of 10% palmyra sap, on the calcium, phosphorus, and phytic acid levels in rice bran. A complete random design was used, comprising three treatments with five replications for each. These treatments included: DN0 (no fermentation), DN6 (6 days of fermentation), and DN12 (12 days of fermentation). The parameters measured were the content of calcium, phosphorus, and phytic acid. The data underwent analysis using variance analysis followed by Duncan’s multiple range test. The findings indicated that fermentation durations led to a reduction in calcium and phytic acid levels, with no notable impact on the phosphorus content in rice bran. Specifically, the 12-day fermentation period yielded the lowest phytic acid content in rice bran.

Assessing solvent impact on Moringa oleifera seed cake nutrition: towards sustainable food and feed production

Abstract Moringa oleifera seed cake, a byproduct of oil extraction, represents a valuable resource for animal and human nutrition. However, solvent selection during extraction significantly influences the cake’s composition. This study aims to evaluates Moringa seed cakes extracted with ethanol, methanol, and hexane, employing proximate analysis, metal content assessment, and microbial testing. The drying process involved hexane seed cake at 55°C, and ethanol and methanol cakes at 70°C for one hour. Proximate analysis indicates that all seed cakes surpass quality standards (SNI, 3571-2009) for ash and crude protein. Hexane seed cake, distinguished by the highest ash (6.4972%) and crude protein (42.1169%), is identified as suitable for bakery and snacks, featuring optimal calcium (0.9697%) and minimal crude fiber (1.9386%), aligning with chicken feed standards (SNI, 8173.2:2015). Methanol seed cake, rich in crude fat (15.7618%) and calories (6549.89 Kcal/Kg), proves suitable for fishery feed. Ethanol seed cake, abundant in crude fiber (30.0319%), is advantageous for calf feed and serves as a foundational ingredient in bakery and snacks. Metal content and microbial contamination adhere to Indonesian standards (SNI, 3751-2009) and (SNI, 3148.2:2009), solidifying Moringa seed cake as a viable alternative across diverse applications.

Effects of genotype and Phytolacca dodecandra (Endod) supplementation on growth performance, carcass traits, blood profiles, and breast meat quality of chickens

In this study, we investigated the effects of genotype and Phytolacca dodecandra (Endod) supplementation on the growth, carcass traits, blood profiles, and breast meat quality of chickens. The study lasted for 18 weeks and involved 360 unsexed day-old chicks divided into nine groups with 40 chicks each, replicated four times with 10 chicks per replication. The genotypes studied were Naked-neck * Tetra H, Normal-feathered * Tetra H, crosses and Tetra H * Tetra H, while the diets included a standard commercial ration (C), Phytolacca dodecandra (Endod) at 1 g/kg (C+1), and Phytolacca dodecandra (Endod) at 2 g/kg (C+2). Results showed that both diet and genotype influenced growth performance indicators like final body weight, body weight change, average daily gain, and feed conversion ratio, particularly during the grower and entire phases. When compared to the control diet, the Phytolacca dodecandra (Endod) chicks supplemented at 1 g/kg showed better performances. The genotypes also affected dressing percentage, breast, thigh, keel bone, and gizzard components, with higher values found in the Naked-neck by Tetra H cross. The supplemented chicken group exhibited an improvement in the dressing percentage and breast muscle in comparison to the control. Blood parameters were also significantly influenced (P < 0.01) by genotype, diet, and their interaction. Supplementation significantly (P < 0.01) increased protein levels while reducing the cholesterol and triglyceride levels in the blood. Incorporating Phytolacca dodecandra (Endod) at a rate of 1 g/kg into the diet of chickens brought a significant improvement in the protein content, and a reduction in the fat content, of their breast muscles. In general, the study indicates that adding up to 2 g/kg of Phytolacca dodecandra (endod) to chicken feed enhances growth performance traits, carcass traits, blood profiles, and breast muscle protein levels, without any negative consequences.

Microbial dynamics and vertical transmission of Escherichia coli across consecutive life stages of the black soldier fly (Hermetia illucens)

BackgroundThe black soldier fly (BSF, Hermetia illucens L.) is one of the most promising insects for bioconversion of organic waste, which often carry a high microbial load with potential foodborne pathogens. Although horizontal transmission (from rearing substrate to larvae) has been extensively studied, less is known about vertical transmission of microorganisms, and particularly of foodborne pathogens, across different BSF life stages.ResultsThis study investigated the microbial dynamics and vertical transmission of Escherichia coli across different life stages (larvae, prepupae, pupae and adults) of one BSF life cycle and its associated substrate (chicken feed) and frass, based on a combination of general microbial counts (based on culture-dependent techniques) and the bacterial community composition (based on 16S rRNA gene sequencing). Multiple interactions between the microbiota of the substrate, frass and BSF larvae were affirmed. The larvae showed relative consistency among both the microbial counts and bacterial community composition. Diversification of the bacterial communities started during the pupal stage, while most notable changes of the microbial counts and bacterial community compositions occurred during metamorphosis to adults. Furthermore, vertical transmission of E. coli was investigated after substrate inoculation with approximately 7.0 log cfu/g of kanamycin-resistant E. coli, and monitoring E. coli counts from larval to adult stage. Although the frass still contained substantial levels of E. coli (> 4.5 log cfu/g) and E. coli was taken up by the larvae, limited vertical transmission of E. coli was observed with a decreasing trend until the prepupal stage. E. coli counts were below the detection limit (1.0 log cfu/g) for all BSF samples from the end of the pupal stage and the adult stage. Additionally, substrate inoculation of E. coli did not have a substantial impact on the bacterial community composition of the substrate, frass or different BSF life stages.ConclusionsThe fluctuating microbial counts and bacterial community composition underscored the dynamic character of the microbiota of BSF life stages. Additionally, vertical transmission throughout one BSF life cycle was not observed for E. coli. Hence, these findings paved the way for future case studies on vertical transmission of foodborne pathogens across consecutive BSF life stages or other insect species.

Evaluation of the Use of Dried Grated Sago as a Substitute Energy Source for Corn in Sentul Chicken Feed

Evaluation of the Use of Dried Grated Sago as a Substitute Energy Source for Corn in Sentul Chicken Feed

Black soldier fly larvae feed suitability for chicken and prospects for economic valuation and viability aspects

Abstract Utilization of black soldier fly larvae (BSFL) emerges as a promising avenue for organic waste recycling and production of protein rich biomass. The current study explored the suitability of BSFL protein feed for chicken and economic facets associated with BSFL rearing facilities, encompassing costs of goods sold (COGs), operating expenditure (OpExp), and capital costs (CapExp). Research revealed that incorporation of BSFL feed for chicken did not exert discernible influences on overall meat quality. However, the amino acid content variation of chicken meat particularly concerning polyunsaturated fatty acids (PUFA), are correlated with the fatty acid composition of the BSFL feed, showed substrate-specific characteristics. The recommended BSFL dosage ranges up to 13% (defatted larvae), 12% (partially defatted BSFL), 14% (dried larvae), and 48% (for whole larvae), which did not affect the chicken body weight and physiological traits, except for the fatty acids profile. However, complete substitution at 100% proved detrimental to body weight. The establishment of a BSFL rearing facility is deemed economically viable, considering large production scales and supply chain development in collaboration with waste material managers. Such collaboration is envisioned to curtail substantial portions of expenses, primarily attributed to transport and labor costs, which constitute 60-70% of the financial outlay in the developing countries context. Based on the operational, capital, and marketing expenditures, the cost for BSFL rearing setup in Asian countries (Pakistan) ranged from US $50,762 to US $100,000, while the estimated profit was US $10,762 to US $51,121. These figures may vary based on cost per product, net price and profit per product, number of products sold, and market share and growth traction facets.

Single-step batch fabrication of microfluidic paper-based analytical devices with a 3D printer and their applications in nanoenzyme-enhanced visual detection of dopamine.

Wax printing is the most widely used method for fabricating microfluidic paper-based analytical devices (μPADs), but it still suffers from disadvantages like discontinuation of wax printers and need for additional equipment for heating treatment. To address these issues, this work initially describes a new class of wax printing approach for high-precision, batch fabrication of μPADs using a household 3D printer. It only involves a one patterning step of printing polyethylene wax into rice paper body. Under optimized parameters, a fabrication resolution, namely the minimum hydrophilic channel width, down to ~189 ± 30 μm could be achieved. In addition, the analytical applicability of such polyethylene wax-patterned μPADs was demonstrated well with enhanced colorimetric detection of dopamine as a model analyte by combining metal-organic framework (MOF) based nanoenzymes (ZIF-67) with a smartphone (for portable quantitative readout). The developed nanosensor could linearly detect dopamine over a concentration range from 10 to 1000 μM, with a detection limit of ca. 2.75 μM (3σ). The recovery results for analyzing several real samples (i.e., pig feed, chicken feed, pork and human serum) were between 91.82 and 102.79%, further validating its good detection accuracy for potential practical applications in food safety and medical diagnosis.

Nanosilver-based materials as feed additives: Evaluation of their transformations along in vitro gastrointestinal digestion in pigs and chickens by using an ICP-MS based analytical platform

The use of a new nanomaterial in the feed chain requires a risk assessment that involves in vitro gastrointestinal digestions to predict its degradation and oral exposure to nanoparticles. In this study, a nanosilver-based material was incorporated into pig and chicken feed as a growth-promoting additive and subjected to the corresponding in vitro gastrointestinal digestions. An inductively coupled plasma mass spectroscopy (ICP-MS) analytical platform was used to obtain information about the silver released in the different digestion phases. It included conventional ICP-MS for total silver determination, but also single particle ICP-MS and coupling to hydrodynamic chromatography for detection of dissolved and particulate silver. The bioaccessible fraction in the intestinal phase accounted for 8–13% of the total silver, mainly in the form of dissolved Ag(I) species, with less than 0.1% as silver-containing particles. Despite the additive behaving differently in pig and chicken digestions, the feed matrix played a relevant role in the fate of the silver.

Emerging mycotoxin occurrence in chicken feed and eggs from Algeria

Poultry farming has developed into one of Algeria’s most productive industrial farming because of the growing demand for sources of protein among Algerian society. Laying hen feed consists mainly of cereals, which can be contaminated with molds and subsequently with their secondary metabolites known as mycotoxins. These later can pose a serious danger to the production and quality of eggs in the commercial layer industry. This work focuses on the detection of emerging mycotoxins, mainly enniatins (ENNs) and beauvericin (BEA), in poultry feed and eggs from different locations in Algeria. Two different QuEChERS-based extractions were established to extract ENNs and BEA from chicken feed and eggs. The determination of mycotoxin occurrence was achieved by a UHPLC-MS/MS method using 0.1% (v/v) formic acid in water and MeOH as mobile phase, an ESI interface operating in positive mode, and a triple quadrupole mass spectrometer operating in MRM for the detection. Matrix-matched calibration curves were carried out for both matrices, obtaining good linearity (R2 > 0.99). The method performance was assessed in terms of extraction recovery (from 87 to 107%), matrix effect (from − 47 to − 86%), precision (RSD < 15%), and limits of quantitation (≤ 1.1 µg/kg for feed and ≤ 0.8 µg/kg for eggs). The analysis of 10 chicken feed samples and 35 egg samples composed of a 10-egg pool each showed that ENN B1 was the most common mycotoxin (i.e., found in 9 feed samples) with contamination levels ranging from 3.6 to 41.5 µg/kg, while BEA was detected only in one feed sample (12 µg/kg). However, eggs were not found to be contaminated with any mycotoxin at the detection limit levels. Our findings indicate that the searched mycotoxins are present in traces in feed and absent in eggs. This can be explained by the application of a mycotoxin binder. However, this does not put a stop on the conduction of additional research and ultimately setting regulations to prevent the occurrence of emerging mycotoxins.

Microwave-Enabled Fast Preparation of a Metal-Organic Framework Hybrid Membrane for Filtration-Enhanced Simultaneous Separation and Detection of Aflatoxin B1.

Mycotoxin contamination in food and the environment seriously harms human health. Sensitive and timely detection of mycotoxins is crucial. Here, we report a dual-functional hybrid membrane with absorptivity and responsiveness for fluorescent-quantitative detection of mycotoxin aflatoxin B1 (AFB1). A biomineralization-inspired and microwave-accelerated fabrication method was established to prepare a hybrid membrane with a metal-organic framework (MOF) loaded in high density. The MOF presented high efficiency in capturing AFB1 and showed fluorescence intensity alteration simultaneously, enabling a dual adsorption-response mode. Deriving from the inherent porous structure of the hybrid membrane and the absorptive/responsive ability of the loaded MOF, a filtration-enhanced detection mode was elaborated to provide a 1.67-fold signal increase compared with the conventional soaking method. Therefore, the hybrid membrane exhibited a rapid response time of 10 min and a low detection limit of 0.757 ng mL-1, superior to most analogues in rapidity and sensitivity. The hybrid membrane also presented superior specificity, reproducibility, and anti-interference ability and even performed well in extreme environments such as strong acid or alkaline, satisfying the practical requirements for facile and in-field detection. Therefore, the membrane had strong applicability in chicken feed samples, with a detection recovery between 70.6% and 101%. The hybrid membrane should have significant prospects in the rapid and in-field inspection of mycotoxins for agriculture and food.

Safety of the feed additive consisting of endo-1,4-β-xylanase (produced with Trichoderma reeseiCBS 143953), subtilisin (produced with Bacillus subtilisCBS 143946) and α-amylase (produced with Bacillus amyloliquefaciensCBS 143954) (Avizyme® 1505) for all poultry species (Danisco (UK) Ltd.).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety of the feed additive consisting of endo-1,4-β-xylanase (produced with Trichoderma reesei CBS 143953), subtilisin (produced with Bacillus subtilis CBS 143946) and α-amylase (produced with Bacillus amyloliquefaciens CBS 143954) (Avizyme® 1505) as a zootechnical feed additive for all poultry species. The additive is authorised in feed for chickens and turkeys for fattening, ducks and laying hens. In 2020, the FEEDAP Panel issued an opinion for the renewal of the authorisation of the additive for the species/categories for which there is an authorisation, a reduction of the minimum recommended level in turkeys for fattening and the extension of use to all poultry species. In that assessment, the Panel could not conclude on the safety of the additive due to uncertainties on the characterisation of the production strains and the possible presence of their viable cells and DNA in the final product. Moreover, limitations were identified in the xylanase specifications and xylanase method of analysis. The applicant submitted information to address the limitations previously identified. The Panel concluded that the additive is safe for the target species under the proposed conditions of use. The use of Avizyme® 1505 in animal nutrition is considered safe for the consumer and the environment. The additive is a mild irritant to skin and eyes; it is not a dermal sensitiser but should be considered a respiratory sensitiser. The additive is efficacious in ducks at 75 U endo-1,4-β-xylanase, 1000 U subtilisin and 100 U α-amylase/kg of complete feed. In other poultry species for fattening (including turkeys), reared for breeding and reared for laying, the additive is efficacious at 187.5 U endo-1,4-β-xylanase, 2500 U subtilisin and 250 U α-amylase per kg of complete feed and at 300 U endo-1,4-β-xylanase, 4000 U subtilisin and 400 U α-amylase per kg of complete feed for all poultry species for laying (except for ducks).

Safety and efficacy of a feed additive consisting of a tincture derived from the leaves of Eucalyptus globulus Labill. (eucalyptus tincture) for all animal species (FEFANA asbl).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a tincture from the leaves of Eucalyptus globulus Labill. (eucalyptus tincture) when used as a sensory additive for all animal species. The product is a ■■■■■ solution, with a dry matter content of ~ 1.86%, which contains on average 0.454% phenolic acids and flavonoids (of which 0.280% was gallic acid), 0.0030% 1,8-cineole and 0.00012% methyleugenol. In the absence of analytical data on the occurrence of mono- or diformylated adducts of acylphloroglucinols with terpenes in the tincture and in the absence of toxicity data, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) could not conclude on the use of eucalyptus tincture for long-living and reproductive animals. For short-living animals (species for fattening), the additive was considered of no concern at 4 mg/kg complete feed for chickens for fattening, 5 mg/kg for turkeys for fattening, 6 mg/kg for piglets and rabbits for meat production, 7 mg/kg for pigs for fattening, 16 mg/kg for veal calves (milk replacer), 14 mg/kg for cattle for fattening, sheep/goats and horses for fattening, and 15 mg/kg for salmonids. These levels were extrapolated to physiologically related minor species. No safety concern would arise for the consumer from the use of eucalyptus tincture up to the levels in feed considered of no concern. Eucalyptus tincture should be considered as irritant to skin and eyes, and as a dermal and respiratory sensitiser. The use of eucalyptus tincture as a flavour in animal feed was not expected to pose a risk for the environment. Since the leaves of E. globulus and their preparations were recognised to flavour food and their function in feed would be essentially the same, no demonstration of efficacy was considered necessary.

The effectiveness of using maggot (Hermetia illucens) as a super native chicken feed ingredient

This study aims to determine the effectiveness of using maggots as a feed ingredient for super-native chickens. The parameters used include feed consumption, weight gain, feed conversion, carcass weight, and abdominal fat. The ingredients used include 30 super native chickens with an age of 12 weeks, feed ingredients in the form of corn, bran, soybean flour, maggot, Gamal leaves, minerals, and premixes. The study used a complete randomized design with 3 treatments repeated 5 times, the treatment included P0 as control without using maggot flour (0%); P1: Maggot flour concentration 10%; and P2: 20% maggot flour concentration. The stages in the study include preparation of ration formulas, maintenance for 4 weeks, and data analysis using analysis of variance (ANOVA). The results showed the highest protein content at 20% treatment at 17.76%, feed consumption, and feed conversion showed non-significant results, but the highest consumption value at P2 was 219.66 gr/week, and the best conversion at P2 was 0.92. Body weight gain, carcass weight, and abdominal fat showed a significant effect, the best value at P2, for weight gain 242 g / 4 weeks, carcass weight 82%, abdominal fat 0.42.

Impact of the inclusion of calcium soap in feed on intestinal histology and blood lipid profile of native chickens

The aim of this study was to examine the impact of the inclusion of calcium soap in feed on the intestinal histology and blood lipid profile of native chickens. This study used 192 native chickens aged 6 weeks with homogeneous body weight which were randomized into 4 types of treatment and 6 replications and each replication used 48 chickens with homogeneous body weight. The four types of treatment were: Group of chickens fed without the addition of Ca-PFAD as a control (chicken Group A); chicken feed with the addition of 2% Ca-PFAD (chicken Group B); chicken feed with the addition of 4% Ca-PFAD (chicken Group C); and chicken feed with the addition of 6% Ca-PFAD (chicken Group D). The results showed that the height of the jejunal villi in chicken groups C and D was significantly (P&lt;0.05) higher than the control (chicken group A). Likewise, the depth of the jejunum crypts of Group C and D chickens was significantly (P&lt;0.05) deeper than that of Group A chickens. The inclusion of 2-6% Ca-PFAD in the feed significantly (P&lt;0.05) improved the blood lipid profile of native chickens. It was concluded that administration of calcium soap (Ca-PFAD) in feed can increase the height of the villi and the depth of the crypts of the intestinal jejunum. On the other hand, at the 4% level, giving calcium soap in feed can reduce cholesterol and Low Density Lipoprotein (LDL) levels, as well as increase triglyceride and High Density Lipoprotein (HDL) levels in native chicken.

Metabolic performance and feed efficiency of black soldier fly larvae.

The black soldier fly (BSF), Hermetia illucens, is used in entomoremediation processes because its larvae can use a variety of organic residues with high efficiency. However, feed efficiencies are variable and characterized by uncertainties. Recently developed growth and metabolic performance models have predicted across different studies that BSF larvae have used 53%-58% of the feed components they have assimilated, in terms of carbon equivalents, for growth throughout their lifetime when reared on chicken feed. This is termed their average net growth efficiency. The remainder of the carbon has been lost as CO2. However, mass balances made under similar conditions show that the weight gained by BSF larvae corresponds to only 14%-48% of the feed substrates removed, indicating substrate conversion efficiency. Both performance indicators show even greater variability if more feed substrates are considered. Feed assimilation and growth rates, costs of growth, maintenance, and larval lifespan have been shown to affect how efficiently BSF larvae convert feed into growth. The differences between average net growth efficiencies and substrate conversion efficiencies further indicate that feed is often not used optimally in entomoremediation processes and that the overall yield of such processes is not determined by larval performance alone but is the result of processes and interactions between larvae, substrates, microbes, and their physical environment. The purpose of this study is to illustrate how quantification of the metabolic performance of BSF larvae can help improve our understanding of the role of the larvae in entomoremediation processes.

Effects of feeding chicken egg yolk antibodies on intestinal cell apoptosis, oxidative stress and microbial flora of tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae

Streptococcosis, the most common bacterial disease of fish in recent years, is highly infectious and lethal, and has become an important factor hindering the healthy and sustainable development of aquaculture. Chicken egg yolk antibody (IgY) has the advantages of high antigen specificity, inexpensive and easy to obtain, simple preparation, no toxic side effects, and in line with animal welfare, which is a green and safe alternative to antibiotics. In this study, the potential of specific IgY in the treatment of gastrointestinal pathogens was explored by observing the effects of specific IgY on intestinal flora, pathological tissue, apoptosis, oxidative stress, and inflammatory response of tilapia. We used the specific IgY prepared in the early stage to feed tilapia for 10 days, and then the tilapia was challenged with Streptococcus agalactiae. The results showed that feeding IgY before challenge had a small effect on the intestinal flora, and after challenge specific IgY decreased the proportion of Streptococcus and increased the diversity of the intestinal flora; in histopathology, specific IgY decreased tissue damage and maintained the integrity of tissue structure.Further study found that specific IgY can reduce intestinal epithelial cell apoptosis and reduce caspase activity; at the same time, the content of MDA was decreased, and the activities of SOD, CAT, GSH-Px and GR were increased. In addition, specific IgY can down-regulate the expression levels of IL-8 and TNF-α genes and up-regulate the expression levels of IL-10 and TGF-β. The results of this study showed that specific IgY could improve the intestinal flora of tilapia infected with Streptococcus agalactiae, reduce intestinal cell apoptosis, oxidative stress injury and inflammatory response, thereby reducing tissue damage and protecting the health of tilapia. Overall, specific IgY can be further explored as a potential antibiotic alternative for gastrointestinal pathogen infections.

Evaluating the Efficiency of Black Soldier Fly (Hermetia illucens) Larvae in Converting Mackerel Head Waste into Valuable Resources.

The seafood processing industry generates significant waste, including mackerel heads (MH), constituting 20-32% of total waste. This study explored the potential of utilizing MH as a feed source for black soldier fly larvae (BSF larvae). BSF larvae are known for their ability to efficiently convert organic materials into nutrient-rich biomass. Five concentrations of MH (0, 10, 20, 30, 40, and 50% in chicken feed) were fed to BSF larvae for eight days. After harvesting, their growth, MH conversion efficiency, nutritional content, and heavy metals reduction potential were measured. BSF larvae showed optimal growth when fed with a feed containing 20% MH, resulting in a 14.36-fold increase in weight compared to the control group, as determined by the Fisher's Least Significant Difference Test. BSF larvae maintained a survival rate of 99.33%. With the lowest feed conversion ratio (FCR) of 2.09 at 20% MH, feed efficiency was improved by up to 65.15%, and feed reduction up to 73.53%. MH enhanced lipid and protein content in BSF larvae. Furthermore, BSF larvae in this study showed higher polyunsaturated fatty acids (PUFA), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as other amino acids which are required for breeding animals. The current study highlights the potential of MH as a feed source for BSF larvae, improving nutritional biomass. It also suggests BSF larvae as an eco-friendly option for handling seafood processing waste and as an alternative feed source for animals.

Production Elasticity of Layer Farming During the COVID-19 Pandemic in Blitar District

The livestock sub-sector, an integral part of the agricultural sector, is the animal protein producing sub-sector which plays an important role in meeting people's nutritional needs. The development of the laying hen farming business has enormous potential, this is because purebred chicken eggs contribute quite a lot to animal protein self-sufficiency. The research aims to analyze the input factors of the production that affect the production of layer farming businesses and to assess the production elasticity of these businesses during the COVID-19 pandemic in Blitar District. Data collection took place between July and August 2020 in the Blitar Distrcit. Utilizing a sample random sampling method. The study involved 64 farmers as representative. The research variables used in this study were the population of laying hens, the price of chicken eggs, feed costs, vaccines and medicines expenses, seed prices, mortality, electricity costs, the price of culled chickens, Hen Day Production (HDP) and the number of workers. Quantitative analysis was employed to analyze production costs, revenues, and income, while qualitative analysis was used to analyze production elasticity through Cobb-Douglas regression analysis. The research data were processed using SPSS version 24. The results of the study indicate that 1) the population of laying hens and HDP significantly affected the production of layer farming businesses, along with the cost of vaccines and medications in the Blitar District. 2) The population of layers, mortality rates, electricity costs, HDP, and total labor demonstrated elasticity with respect to the production of layer farms. Conversely, egg prices, feed costs, vaccination and medication expenses, day-old chick (DOC) costs, and final chicken prices exhibited inelasticity concerning the production of layer farms in Blitar district.

Identification of molds producing aflatoxin from raw materials of free-range chicken feed in East Nusa Tenggara, Indonesia

Identification of molds producing aflatoxin from raw materials of free-range chicken feed in East Nusa Tenggara, Indonesia