The biological control agent Bacillus thuringiensis (Bt) coordinates metabolic and gene regulation functions to enter to the differentiation process for the synthesis of spores and Cry toxin proteins. The master regulator for sporulation initiation is Spo0A, while AbrB regulator coordinate cellular functions during vegetative and transition growth. To understand how differentiation is modified during fed-batch cultures (FBC) of Bt under variable nutrient conditions, we conducted fermentations with two different nutrient concentration and feeding times using an industrial medium. Since sporulation efficiency has been lower in FBCs than in batch cultures, AbrB was monitored by Western blot during these cultures. Kinetic parameters were used to stablish timing of cell differentiation process, and fermentations were monitored on-line using impedance spectroscopy. AbrB was accumulated during the entire fed-batch culture, when a medium with the highest concentration of carbon and nitrogen sources (FBC1) were fed. Under these conditions, sporulation efficiency and cry1Ac expression were negatively affected, compared to the wild-type strain. Conversely, when the feeding concentration was only half of FBC1, AbrB accumulates in minor proportion, and sporulation efficiency and cry1Ac expression increased (FBC2). These results suggest that variable nutrient conditions can cause an imbalance in the accumulation of transition state regulators such as AbrB during FBCs, which can be detrimental to produce spores and Cry proteins, that are the main Bt products for biological control of agricultural pests.