Model-based definition (MBD) is a new strategy of managing engineering and business processes using 3D models as complete information sources. However, due to lack of feature-based data model representation approach, current MBD models cannot be applied well in manufacturing domain. In this paper, a new multi-level structuralized MBD model based on machining features for manufacturing reuse is presented to capture the abstract information, detailed feature interaction information, and machining semantics information involved. Firstly, the machining features are recognized from an MBD model, which are taken as the machining semantics carrier. Then, the coupled machining feature cluster is introduced to construct the greater granularity’s independent structural element than machining feature, which describes the feature interactions. Finally, the MBD model is structured based on machining features and coupled machining feature clusters in a hierarchical way. In the experiments, some aircraft structural parts are utilized to verify our approach for manufacturing reuse, and the experimental results are analyzed at length to show the application effectiveness of the multi-level structuralized MBD model.