Feather Pecking Research Articles

Non-infectious diseases and laying hen welfare

Numerous non-infectious conditions can affect commercial layer flocks. Some of these, such as conditions related to bodyweight, fatty liver haemorrhagic syndrome and effects of age are common to all production systems, while some are more common in cage systems (cage layer fatigue, feather pecking) or in cage-free systems (grass impaction, smothering, sunlight exposure, cannibalism, predation and injuries). Many of these conditions are covered in other papers and will not be dealt with in here. In the present paper, emphasis will be placed on grass impaction, smothering, effects of age, exposure to high light intensities, predation and conditions related to overweight/obesity issues. All of these are of considerable welfare concern for the industry. Grass impaction is a particular issue when hens are first released to the range areas and are exposed to vegetation or other fibrous materials. Mortality can be high with this problem. Smothering episodes can be generated by sudden flock disturbances, or from overcrowding in nests or related to nest-box design. Other less well understood triggers for smothering are poorly understood but have been linked to temperature variability, retreating shade in range areas on sunny days, dust-bathing opportunities and things that attract birds to certain points in the shed. The incidence of tumours increases with hen age. Most frequently encountered tumours are leiomyomas and adenocarcinomas, both of which are associated with the reproductive tract and are presumably linked to selection for higher and more sustained egg production. The incidence of these neoplastic conditions will need better understanding if increases in flock persistency of lay are desired. Light intensity is understood to be a possible trigger of increased aggressive behaviour and can lead to increased feather pecking and cannibalism. Predation, while frequently reported, is not regarded as a major cause of loss in most farming circumstances, although individual cases can be serious and it is a major cause of concern. Obesity is becoming more recognised as a problem in layer flocks as research begins to focus on its effects. Reduction in average flock weights will be an objective for further improvement in persistency of lay in the future.

Corticosterone in feathers: Inter- and intraindividual variation in pullets and the importance of the feather type

Measuring corticosterone concentrations in feathers of poultry may be suitable to determine birds' exposure to stress. It is thinkable, that in laying hens such information could be helpful as an animal welfare indicator to evaluate adverse husbandry conditions and to predict the risk of developing behavioral disorders, such as feather pecking and cannibalism. Yet, there are some fundamental issues which remain unclear. Therefore, the objective of the current pilot study was to examine the inter- and intraindividual variation of pullets at the end of the rearing period, when most of the feathers are fully grown and animals are reaching sexual maturity. Flight feathers from both wings (n = 4), the tail (n = 2 – 3), and body feathers (n = 1 pool of 3 – 5 feathers) were taken from pullets (n = 10), genetics Lohmann Brown, at an age of 19 weeks who were reared in the same flock (N = 728). Corticosterone analysis was performed applying a validated protocol for laying hens. Results indicate not only high intraindividual, but also high interindividual variation. Mean over all samples was 75.2 pg/mg (± 38.58 pg/mg, n = 76), showing higher intraindividual variation (between feather types; SD: 23.75 pg/mg – 49.38 pg/mg; n = 10 pullets) than interindividual variation (within feather types; SD: 11.91 pg/mg – 49.55 pg/mg; n = 6 feather types). The variation between different feather types within one bird was higher than the variation within one feather type between different birds, indicating that birds a) may respond differently when exposed to stressors and b) corticosterone measurements should be done with the same feather type.

Runs of Homozygosity in Modern Chicken Revealed by Sequence Data.

Runs of homozygosity (ROH) are chromosomal stretches that in a diploid genome appear in a homozygous state and display identical alleles at multiple contiguous loci. This study aimed to systematically compare the genomic distribution of the ROH islands among five populations of wild vs. commercial chickens of both layer and broiler type. To this end, we analyzed whole genome sequences of 115 birds including white layer (WL, n = 25), brown layer (BL, n = 25), broiler line A (BRA, n = 20), broiler line B (BRB, n = 20) and Red Junglefowl (RJF, n = 25). The ROH segments varied in size markedly among populations, ranging from 0.3 to 21.83 Mb reflecting their past genealogy. White layers contained the largest portion of the genome in homozygous state with an average ROH length of 432.1 Mb (±18.7) per bird, despite carrying it in short segments (0.3-1 Mb). Population-wise inbreeding measures based on Wright’s (Fis) and genomic (FROH) metrics revealed highly inbred genome of layer lines relative to the broilers and Red Junglefowl. We further revealed the ROH islands, among commercial lines overlapped with QTL related to limb development (GREM1, MEOX2), body weight (Meis2a.1, uc_338), eggshell color (GLCCI1, ICA1, UMAD1), antibody response to Newcastle virus (ROBO2), and feather pecking. Comparison of ROH landscape in sequencing resolution demonstrated that a sizable portion of genome of commercial lines segregates in homozygote state, reflecting many generations of assortative mating and intensive selection in their recent history. In contrary, wild birds carry shorter ROH segments, likely suggestive of older evolutionary events.

Phenotypic and genomic analyses of agonistic interactions in laying hen lines divergently selected for feather pecking

In poultry, aggressive pecking and threatening are normal agonistic behavior patterns which serve to establish a social hierarchy. As agonistic behavior is a stressor for animals, its excessive occurrence is undesired in layer flocks. Feather pecking is a longstanding serious problem in layer flocks and its relationship to agonistic behavior is still not clear. Therefore, phenotypic and genomic analyses of the agonistic and feather pecking behavior of two laying hen lines divergently selected for high and low feather pecking were conducted. The hens were phenotyped for the active traits aggressive pecks delivered (APD)11Aggressive pecks delivered (APD), threats delivered (TD)22Threats delivered (TD), feather pecks delivered (FPD)33Feather pecks delivered (FPD) and the passive traits aggressive pecks received (APR)44Aggressive pecks received (APR), threats received (TR)55Threats received (TR) and feather pecks received (FPR)66Feather pecks received (FPR). Indices were built by subtracting the passive traits from the respective active traits to obtain the aggression index, the threat index, and the feather pecking index. As all three behavior patterns in their excessive manifestations are undesired, the index-traits Activity and Passivity were also defined by combining each the active and passive traits. The results showed that FPD is significantly positive correlated with APD and TD in both lines, but with higher coefficients in the high feather pecking line. The average amount of FPR in both lines is nearly the same and no correlation was found between FPR and FPD, APD or TD in any of the lines. The active traits and the feather pecking index showed medium heritabilities, whereas the heritability was negligibly small for the other traits. GWAS revealed four nominal significant (p ≤ 5*10−5) SNPs for APD on chromosome 6, the same four and three additional SNPs on chromosome 8 for Activity and three SNPs on chromosome 1 for the feather pecking index. It is concluded, that selection on high feather pecking leads to an increase of agonistic behavior. The correlation probably depends on the phase of establishing the social hierarchy in which the hens in a newly formed group are at the time of observation, and might disappear, after a stable ranking is established. The reception of feather pecking is similar in both lines. GWAS revealed that TD, APD, Activity and the feather pecking index seem to be typical quantitative traits with associated regions for the latter three which have slightly greater effects on these traits than other regions in the genome.

Litter and perch type matter already from the start: exploring preferences and perch balance in laying hen chicks

Early chick environment, such as provision of litter and perches, can be a predictor of laying hen welfare. Inadequate or nonpreferred litter and perch types could have similar negative effects as those seen when not providing these resources at an early stage, such as increased feather pecking and cannibalism in adult flocks. However, suitable litter and perch types for chicks are not well explored. In the present project, 6 different types of litter (crushed straw pellets, hemp shavings, peat, sand, straw, wood shavings) and 6 different types of perches (narrow or wide forms of rope, flat or round wood) were presented in a controlled way (3 at a time) to chicks in 6 pens. Usage was compared in 93 chicks of Lohmann Selected Leghorn Classic divided across the pens, during their first 3 wk after hatch. Different litter types were seen to be preferred for different behaviors. The majority of dustbathing bouts occurred in sand and peat. Chicks foraged more in wood shavings, hemp shavings, and sand than in peat and pellets (P < 0.05). Perch width and shape were found to affect both usage and perch balance, measured as the probability of successful or problematic landings. The wide rope was generally used more during the first week (P < 0.05) and was used more for sleeping or resting (P < 0.05) than the other wide perch types. Furthermore, birds were more likely to land on the wide rope or on flat perches successfully than they were to have a problematic landing (P < 0.05). That birds were more likely to be observed preening on flat perches than on the potentially shaky rope perches could further reflect a sense of security. Our results suggest that presenting several litter types could better fulfill chicks' behavioral needs and that flat perches or a wide rope (4.5-cm diameter) could be appropriate perch types for laying hen chicks and thereby promote early perch use and training.

A meta-analysis on the effect of environmental enrichment on feather pecking and feather damage in laying hens

Feather pecking (FP) is a significant issue in laying hens, which impacts societal acceptance of poultry farming, farm productivity, and bird welfare. Environmental enrichment—modifications of the environment to stimulate biological functioning and psychological well-being of animals—is one management strategy farmers can use to mitigate FP. However, inconsistent results of environmental enrichment are reported across studies questioning its value. A meta-analysis was used to determine the effect of environmental enrichment on FP and feather damage (FD) in laying hens. A systematic review of published literature from 4 databases resulted in 23 publications that met inclusion criteria. Feather pecking and FD outcomes were standardized between studies using different scoring systems. Driving variables included the presence of enrichment, production period when the enrichment started, housing type, beak trimming, bird strain, and age of the birds when FP and FD was measured. Considering the experiment as a random effect, linear mixed model analysis was used in a 2-step approach, whereby variables with a P < 0.30 in univariate analysis were included within the subsequent multivariate analysis. Variables with P < 0.05 in the multivariate analysis were retained in the final models. Model selection and evaluation were based on corrected Akaike information criteria, the root mean square prediction errors, and concordance correlation coefficients. A higher frequency of FP was observed in flocks lacking enrichment (P < 0.001), with increased age (P = 0.001), and in cage housing systems (P = 0.002). Similarly, FD increased in flocks lacking enrichment (P = 0.018), with increased age (P < 0.001), in the absence of beak trimming (P = 0.001) and in cage housing systems (P = 0.042). This meta-analysis confirmed the effectiveness of environmental enrichment in reducing FP and FD. Nevertheless, the modest ability of enrichment to dampen FD (−0.14 ± 0.06, 1-4 scale) suggests that other management strategies must be implemented in conjunction with environmental enrichment to successfully manage FP and resulting FD.

Assessment of laying-bird welfare following acaricidal treatment of a commercial flock naturally infested with the poultry red mite (Dermanyssus gallinae).

The poultry red mite (PRM), Dermanyssus gallinae, a potential vector of pathogens to animals and humans, causes impaired bird welfare. A study investigated changes in behavioural variables, physiological biomarkers, and health parameters following acaricidal treatment of PRM infestation of laying hens on a commercial farm. Mite traps determined the challenge to 12,700 hens before and after drinking water administration of the acaricide, fluralaner (Exzolt®, 0.5 mg/kg; Weeks 0 and 1). Weekly daytime direct observations and night-time video recordings monitored bird behaviours from Weeks -6 through +6. Blood samples were collected from randomly-selected birds (Weeks -6, -1, and +6). Following treatment, mite count reductions (>99%) were statistically significant (P < 0.0001), as were night-time reductions in the percent of hens showing activity, preening, head scratching (all P < 0.0001), and head shaking (P = 0.0007). Significant daytime reductions were observed in preening and head scratching (both P < 0.0001), head shaking (P = 0.0389), severe feather pecking (P = 0.0002), and aggressive behaviour (P = 0.0165). Post-treatment, comb wounds were significantly reduced (P = 0.0127), and comb colour was significantly improved (P < 0.0001). Heterophil/lymphocyte ratio was significantly reduced at Weeks 1 and 6 (P = 0.0009 and P < 0.0001, respectively). At Week 6, blood corticosterone (P = 0.0041) and total oxidant status (P < 0.0001) were significantly reduced, and haemoglobin and mean corpuscular haemoglobin significantly increased (P < 0.0001). Farm production records indicated that those post-treatment improvements were accompanied by significant reductions in weekly mortality rate (P = 0.0169), and significant recovery in mean weekly egg weights (P < 0.0001) and laying rate (P < 0.0001). The improvements in behavioural variables, physiological biomarkers, and health parameters that were observed following the elimination of PRM on a commercial farm indicate that infestations can be a cause of reduced hen welfare.

Husbandry Conditions and Welfare Outcomes in Organic Egg Production in Eight European Countries.

Simple SummaryAn important challenge for the further development of organic egg production is to reach and maintain a high level of animal welfare. In a European research project including eight countries, the welfare state in 107 laying hen flocks as well as their housing and management were recorded during two farm visits at peak and end of lay. Data analysis aimed to reveal factors that may help to prevent or reduce welfare problems. Large variation between flocks indicated options for improvement with regard to mortality, feather and injurious pecking, parasitic infestation and keel bone damage. Results of the project indicate that (i) decreasing mite and worm infestation and (ii) providing an attractive covered veranda help to decrease mortality; (iii) maximising access to the free-range to decrease injurious pecking and Ascaridia galli infection; (iv) feeding sufficient protein levels and (v) providing adequate litter is preventive against feather pecking and cannibalism; (vi) ensuring that the birds have sufficient weight and (vii) preventing accidents by adequate hen house facilities and light conditions contributes to reduced keel bone damage. These primarily management-based measures have the potential to improve bird welfare both in terms of behavioural and health aspects in organic egg production systems.In the European research project HealthyHens, welfare indicators as well as husbandry and management conditions were recorded in 107 organic laying hen farms in eight countries. Farms were visited at peak and end of lay. Egg production was on average comparable to breeder specifications. A mean mortality of 5.7% and mean prevalences of footpad lesions of 30.5%, keel bone damage of 44.5%, 57.3% of flocks with on average >200 Ascarid eggs per gram faeces and 28.2% of flocks with >100 mites/trap were recorded. A large variation between flocks indicated options for improvement. Based on the results, the following measures can be recommended: (i) decreasing mite and worm infestation and (ii) providing an attractive covered veranda, because of their association with decreased mortality; (iii) maximising access to the free range, because of its relation to decreased A. galli infection and less injurious pecking; (iv) feeding sufficient protein levels and (v) providing adequate litter as preventive measure against feather pecking and cannibalism; (vi) ensuring that the birds have sufficient weight and (vii) preventing accidents by adequate hen house facilities and light conditions to reduce keel bone damage. These primarily management-based measures have the potential to improve bird welfare both in terms of behavioural and health aspects.

Dual-purpose production of eggs and meat\u2014part 2: hens of crosses between layer and meat breeds show moderate laying performance but choose feed with less protein than a layer hybrid, indicating the potential to reduce protein in diets

The use of modern chicken genotypes with high egg or meat performance results in the ethically unacceptable practice of culling day-old male layer chicks because of their inefficient fattening performance. Dual-purpose genotypes with a balanced performance profile for eggs and meat are one option to avoid this practice. In this study, the performance of four crosses of a layer breed (White Rock or New Hampshire) and the meat breed Bresse Gauloise, purebred Bresse Gauloise and the layer hybrid Lohmann Sandy was compared under organic husbandry conditions. Part 2 focuses on the laying performance of the hens until the age of 72 weeks and their feed choices when offered energy-rich and protein-rich feed mixtures. Feed consumption was generally high (133–143 g day−1), but the crosses consumed less protein feed than Lohmann Sandy, indicating a potential to reduce the proportion of high-protein components. Bresse Gauloise × White Rock showed severe plumage loss due to feather pecking, which was most likely caused by their low consumption of protein feed. Apparently, free-choice feeding did not agree with this genotype. Average laying performance of the crosses was 68–73% total eggs per average hen, which can be considered moderate. Average egg weights and egg size distribution were comparable with Lohmann Sandy, while slaughter performance of the senior hens was comparable with purebred Bresse Gauloise. Based on these findings, egg production with dual-purpose laying hens can be an ethically desirable alternative to high-performance genotypes, but will require further research on optimal feeding strategies and animal behaviour.Trial registered on July 7, 2017 (reference number V 241-26532/2017)

Meta-analyses of genome wide association studies in lines of laying hens divergently selected for feather pecking using imputed sequence level genotypes

BackgroundFeather pecking (FP) is damaging behavior in laying hens leading to global economic losses in the layer industry and massive impairments of animal welfare. The objective of the study was to discover genetic variants and affected genes that lead to FP behavior. To achieve that we imputed low-density genotypes from two different populations of layers divergently selected for FP to sequence level by performing whole genome sequencing on founder and half-sib individuals. In order to decipher the genetic structure of FP, genome wide association studies and meta-analyses of two resource populations were carried out by focusing on the traits ‘feather pecks delivered’ (FPD) and the ‘posterior probability of a hen to belong to the extreme feather pecking subgroup’ (pEFP).ResultsIn this meta-analysis, we discovered numerous genes that are affected by polymorphisms significantly associated with the trait FPD. Among them SPATS2L, ZEB2, KCHN8, and MRPL13 which have been previously connected to psychiatric disorders with the latter two being responsive to nicotine treatment. Gene set enrichment analysis revealed that phosphatidylinositol signaling is affected by genes identified in the GWAS and that the Golgi apparatus as well as brain structure may be involved in the development of a FP phenotype. Further, we were able to validate a previously discovered QTL for the trait pEFP on GGA1, which contains variants affecting NIPA1, KIAA1211L, AFF3, and TSGA10.ConclusionsWe provide evidence for the involvement of numerous genes in the propensity to exhibit FP behavior that could aid in the selection against this unwanted trait. Furthermore, we identified variants that are involved in phosphatidylinositol signaling, Golgi metabolism and cell structure and therefore propose changes in brain structure to be an influential factor in FP, as already described in human neuropsychiatric disorders.

Effects of edible environmental enrichments during the rearing and laying periods in a littered aviary—Part 2: physical development of pullets and performance, egg quality, and carcass composition in laying hens

Feather pecking and cannibalism are 2 major problems in laying hens' husbandry. Although additional environmental enrichment material (EM) supply is thought to lessen these problems, consistent evidences are lacking. This study examined the effects of EM supply (pecking stones and alfalfa bales) on biological performance, carcass composition, and animal losses in a littered housing system. 2,000 brown-egg and 2,000 white-egg layer hen pullets of the genetic strains Lohmann Brown classic and Lohmann Selected Leghorn classic, respectively, were reared separately in a 16-compartment aviary system until week 18. 1,320 remaining laying hens were then transferred to a 44-compartment laying stable (weeks 19–48). Both strains were tested under 4 treatment variants (n = 150–180 per strain and per variant): V1—no EM over the entire study period; V2—the rearing period with and the laying period without EM; V3—the rearing period without and the laying period with EM; V4—EM over the entire study period. Data on development, performance, egg quality, feed intake, EM consumption, animal losses, carcass composition, and economic traits were collected. Enrichment material supply during rearing (V2 and V4, both strains) was found to globally increase not only hens' relative gizzard mass (P = 0.036) but also the cracked eggs' percentage (compared with V3; P = 0.008) and to decrease the body mass in weeks 6 (P = 0.023) and 8 (P = 0.023) and the uniformity in week 16 (P = 0.011). Enrichment material provision during the laying period (V3 and V4, both strains) increased egg weights (P = 0.028) and the mean body mass (P = 0.036); however, continuous provision of EM (V4, both strains) increased the floor eggs' percentage (P = 0.019). The EM supply did not affect mortality, loss of production days, losses due to skin and toe cannibalism, or the income over feed costs. However, the income over feed and enrichment costs of V1 hens was higher by 0.55 €/hen than that of V4 hens (P = 0.022). Therefore, EM supply cannot be recommended as a measure to increase laying performance and reduce animal losses, but its positive effects on animal welfare should be considered.

Analysis of the brain transcriptome in lines of laying hens divergently selected for feather pecking

BackgroundFeather pecking (FP) in laying hens reduces animal welfare and leads to economic losses for the layer industry. FP is considered a heritable condition that is influenced by dysregulation of neurotransmitter homeostasis, the gut microbiome, and the immune system. To identify genes and biological pathways responsible for FP behavior we compared the brain transcriptomes of 48 hens divergently selected for FP. In addition, we tested if high feather peckers (HFP) and low feather peckers (LFP) respond differently to light since light has been shown to trigger FP behavior.ResultsOf approximately 48 million reads/sample an average of 98.4% were mapped to the chicken genome (GRCg6a). We found 13,070 expressed genes in the analyzed brains of which 423 showed differential expression between HFP and LFP. Genes of uncertain function and non-coding RNAs were overrepresented among those transcripts. Functional analyses revealed the involvement of cholinergic signaling, postsynaptic activity, membrane channels, and the immune system. After the light stimulus, 28 genes were found to be differentially expressed. These included an interaction cluster of core components of the circadian clock. However, differences in the response to light between HFP and LFP were not detectable.ConclusionsGenes involved in cholinergic signaling, channel activity, synaptic transmission, and immune response were found to be involved in FP behavior. We propose a model in which the gut microbiota modulates the immune system, which in turn affects cholinergic signaling. This might have an influence on monoamine signaling with possible involvement of GABA or glutamate signaling.

Causes of feather pecking and subsequent welfare issues for the laying hen: a review

When feather-pecking behaviour by hens becomes repetitive, plumage damage often results for the recipient of the pecks. The forceful removal of feathers and vigorous pecks directed at the skin may also cause pain, fear and even wounds. ‘Outbreaks’ of pecking behaviour have been reported in all housing systems in which poultry are managed. Pecking may progress to cannibalism and death, thus constituting significant hen welfare and farm economy problems. Farmers apply preventative management practices to minimise the risk of outbreaks. However, outbreaks are unpredictable and, once in progress, are difficult to control, especially in non-cage housing systems. For more than a century, research has been directed at trying to identify the causal factors underlying this problem, without success. The problem is multi-factorial and different studies often identify contradictory findings, such as, for example, in relation to the effects of adding forage to increase environmental enrichment, among others. The present review aims to provide background information about severe feather-pecking behaviour in laying hens, with mention of the resultant issues from repeated performance, such as, for example, on feather cover over the life of the laying hen. On-farm surveys, epidemiological studies and experimental trials have generated much information that has improved our general understanding of the significance of the problem, even though studies have typically been inconclusive due to its multi-factorial causes. While ‘Good Practice Guides’ are available and provide relevant advice for farmers to manage flocks to minimise the risk of outbreaks, we suggest significant progress towards identifying the root-cause(s) of the problem will more likely be achieved through controlled experimental trials using research models than through survey approaches. For example, using a stress-induction model, researchers should first focus on the impact of cumulative stressors in the flock that seem to predispose a hen to either become a feather pecker, or be the victim of pecking. Subsequent research should then investigate the affected hens for altered behavioural or (neuro-) physiological states, or physical stimuli on the skin and feathers, that may increase the motivation of hens to become feather peckers.

Welfare implications for barn (and aviary) egg production systems

Barn production systems in Australia are either an indoor-housing floor system or an aviary system with multilevel structures, equipped with nesting boxes, perches and feeding and watering systems. These systems offer hens the freedom of movement and an opportunity to display a repertoire of favourable behaviours as well as interact with complex housing elements as compared with caged systems. However, the system can create prospects for the hens to express detrimental behaviours such as feather pecking and cannibalism. Other aspects of welfare that may be compromised when compared with cage systems include incidence of fractures and injuries during navigation of hens among elements of housing that may result in collision or falls. Barn production systems may have an advantage over free-range production systems in relation to the protection they offer from predators and predator stress, and diseases and parasites that can be contracted from range areas. Barn systems also offer better biosecurity due to lack of direct access to wild birds and their faeces. The aim of the present review is to assess the welfare status of birds housed in barn (and aviary) production systems, while considering husbandry factors that affect welfare outcomes, the health and disease implications and sustainability. To maintain a high welfare outcome in barn production systems, it is important to keep the enclosed environmental conditions optimum by managing air quality, ventilation and lighting within sheds. Sustainability of these production systems depends on consumer preference, cost of production, environmental footprint and suitable genetics of hens.

Phylogenetic Inference Using Cytochrome C Oxidase Subunit I (COI) in the Poultry Red Mite, Dermanyssus gallinae in the United Kingdom Relative to a European Framework.

The poultry red mite (Dermanyssus gallinae), an obligatory blood feeding ectoparasite, is primarily associated with laying hens where it is estimated to cause losses of ~€231 million per annum to European farmers. Moderate to high infestation levels result in negative impacts on hen welfare, including increased cannibalism, irritation, feather pecking, restlessness, anemia, and mortality. Acaricides are currently the prevailing method of population control for D. gallinae, although resistance against some classes of acaricide has been widely reported. The development of resistance highlights a growing need for research into alternative control methods, including the development of a suitable and effective vaccine. Understanding the genetic structure of D. gallinae populations can support improved management of acaricide resistance and sustainability of future vaccines, but limited data are currently available. The aim of this study was to characterize D. gallinae isolates from Europe, targeting the cytochrome c oxidase subunit 1 (COI) gene to gain an insight into population structure and genetic diversity of currently circulating mites. Dermanyssus gallinae isolates were collected from Albania, Belgium, Croatia, Czech Republic, Denmark, France, Greece, Italy, the Netherlands, Portugal, Romania, Slovenia, Turkey and the United Kingdom. Genomic DNA was extracted from individual adult D. gallinae mites and a 681bp fragment of the COI gene was amplified and sequenced. Phylogenetic analyses of 195 COI sequences confirmed the presence of multiple lineages across Europe with 76 distinct haplotypes split across three main haplogroups and six sub-haplogroups. Importantly there is considerable inter- and intra-country variation across Europe, which could result from the movement of poultry or transfer of contaminated equipment and/or materials and husbandry practices.

Current methods and techniques of beak trimming laying hens, welfare issues and alternative approaches

Beak trimming is used in the egg industry to prevent mortality from cannibalism and minimise injurious pecking, vent pecking, aggressive pecking at the head and all forms of feather pecking, although the practice does not completely reduce the damage. There are alternatives to beak trimming, but they have not been reliable in preventing injurious pecking. However, beak trimming should not be used without providing birds enriched indoor and outdoor environments. Even when birds are beak trimmed, providing enriched facilities is recommended. The two main methods of beak trimming are hot blade (HB) and infrared beak treatment (IRBT). HB trimming removes the beak tips and cauterises the beak stump. The IRBT method uses heat from an infrared lamp to treat the outer beak and the underlying tissue. Initially, the tips of the beak remain intact and then soften and wear as the bird uses its beak. In contrast, the HB method results in an open wound that takes 3 weeks to heal. Two major welfare issues arise from beak trimming. The first is loss of sensory input because of removal of or heat treatment of sensory receptors in the beak. The second issue is the potential for acute and chronic pain from severing or heat treatment of nerves. HB trimming initially results in acute pain but there is limited evidence for beak sensitivity in IRBT birds. The development of neuromas in the beak have been implicated as a cause of chronic pain after HB trimming. When birds are HB trimmed (one-half of upper beak; one-third of lower) in the first 10 days of life, neuromas will develop, but they will resolve, compared with birds trimmed at older ages. However, neuromas will not resolve in 10-day old birds if more than one-half of the beak is HB trimmed. While HB trimming is performed according to accreditation standards by removal of one-half the upper beak and one-third of the lower beak is considered excessive, it prevents beak regrowth and the need to subject birds to re-trimming and a second bout of acute pain from the beak wound. Current levels of IRBT to treat half of the beak using the vendors quality-assurance protocol have been implemented worldwide to ensure that neuroma formation is prevented; however, if severe levels of IRBT are used, acute pain and subsequent neuromas may persist. The main impact of beak trimming is how the bird uses its beak when it eats, drinks and pecks at other birds and the environment. Reduced feed intake after HB trimming indicates pain associated with pecking or difficulty in eating. HB-trimmed birds initially show an increase in listlessness and guarding behaviours and increased inactivity, which indicates pain. IRBT and HB-trimmed birds have fewer aggressive pecks at the head, and there is a reduction in severe feather pecking and better feather scores, which results in a large reduction in mortality. Depending on the severity of IRBT and HB trimming, the advantage of using IRBT is improved welfare.

Effects of Dimethyl Anthranilate-Based Repellents on Behavior, Plumage Condition, Egg Quality, and Performance in Laying Hens.

Feather pecking is a behavior that occurs in order to cope with a constrained environment and is a serious problem in the egg production industry. This longitudinal study was conducted under commercial conditions to investigate whether the application of two repellent mixtures, previously suggested as aversive to wild birds, to the plumage of Prelux-R hybrid egg layers is a viable alternative to beak trimming as a solution to discourage feather pecking among laying hens. A total of 180 untrimmed hybrid layers was reared together in a floor pen. At 18 weeks of age they were allocated randomly to three treatments (repellent P, repellent T, control), each consisting of 6 replicated enriched cages with 10 hens in each cage. Hens were evenly sprayed once every 2 weeks for 54 weeks with solution P (dimethyl anthranilate and methyl phenylacetate), solution T (dimethyl anthranilate and geraniol), or distilled water (control). Body weight, plumage condition, behavior, feed intake, and egg quality measurements were taken at five time periods from 26 to 76 weeks of age. Egg production and mortality were recorded daily. The treatments did not affect feather pecking behavior. Hens treated with repellent T tended to perform less cage pecking than the control hens. The use of the repellents did not reduce feather pecking, the plumage was even more significantly damaged in the hens given the repellents compared to the control hens. This suggests the chemicals in the repellents worsened the plumage. No differences in feed intake and daily egg production between treatments were found. Raw and hard-boiled eggs were highly uniform in odor/flavor/taste and no offensive odor absorption related to the chemicals in the repellents was detected. In conclusion, in the present study we did not find any beneficial effect of dimethyl anthranilate-based repellents on feather pecking frequency and plumage/feather condition. Therefore, we do not encourage their use in wider commercial settings.

Effects of edible environmental enrichments during the rearing and laying periods in a littered aviary—Part 1: integument condition in pullets and laying hens

Severe feather pecking is a damaging allopecking behavior in pullets and laying hens which was found to be associated with multiple factors. The aim of this study was to investigate whether the occurrence of feather pecking could be reduced by additional environmental enrichment materials (EM; pecking stones and hard-pressed alfalfa bales) in a littered housing system. In total 4,000 pullets (2000 Lohmann Brown classic [LB] and 2000 Lohmann Selected Leghorn classic [LSL]) were reared in an aviary system until week 18, and 1,320 remaining laying hens were kept thereafter, from week 19 to 48. During rearing, half of the compartments were permanently supplied with enrichment materials, while the other half did not receive any EM. After transferring to the laying hens’ stable, we examined the hens under four treatment variants: V1 - no EM over the entire study period; V2 - rearing period with and laying period without EM; V3 - rearing period without and laying period with EM; V4 - EM over the entire study period. The integument condition (i.e. beak protrusion, beak fissures, plumage condition, skin and toe injuries, and foot pad dermatitis) was scored in weeks 2, 4, 6, 10, 14, 18 (rearing) and 25, 30, 40, and 48 (laying period). At the end of the study (week 48), lower plumage damage was observed in the variants provided with EM during rearing (V2 and V4) compared to the other two variants (V1 and V3; P < 0.001). Skin injuries were less prevalent in week 40 in hens with access to EM during rearing (V2 and V4) than the other variants (V1 and V3) within LSL (P < 0.001), and in week 48 in the both strains (P < 0.001). The variants with access to EM during the laying period (V3 and V4) showed lesser protrusion of the beak tip in week 48 (P < 0.001). The observed beneficial effects of EM could be attributed to its impact on foraging behavior and beak-tip abrasion.

Cecal motility and the impact of Lactobacillus in feather pecking laying hens

The gut-microbiota-brain axis is implicated in the development of behavioural disorders in mammals. As such, its potential role in disruptive feather pecking (FP) in birds cannot be ignored. Birds with a higher propensity to perform FP have distinct microbiota profiles and feed transit times compared to non-pecking counterparts. Consequently, we hypothesize that the gut microbiota is intimately linked to FP and gut motility, which presents the possibility of using probiotics to control FP behaviour. In the present study, we aim to assess the relationship between cecal motility and the probiotic Lactobacillus rhamnosus in chickens classified as peckers (P, 13 birds) and non-peckers (NP, 17 birds). We show that cecal contractions were 68% less frequent and their amplitude increased by 58% in the presence of L. rhamnosus. Furthermore, the number of FP bouts performed by P birds was positively correlated with contraction velocity and amplitude. We present the first account of gut motility measurements in birds with distinct FP phenotypes. Importantly, the present work demonstrates the clear impact of a probiotic on cecal contractions. These findings lay the foundation for identifying biological differences between P and NP birds which will support the development of FP control strategies.

UK consumers' willingness to pay for laying hen welfare

PurposeThis study investigates UK consumers’ perception and willingness to pay (WTP) for egg attributes associated with laying hen welfare, namely with beak-trimming practices and injurious feather pecking (IP). The aim is to examine any change in WTP after improved consumer awareness.Design/methodology/approachBuilding upon existing literature, the authors designed an online survey in which the method of discrete choice experiment (DCE) was employed. The study includes two identical DCEs with the second being introduced after respondents were presented with an educational excerpt about beak-trimming practices, on farm IP occurrence and the docile nature of white egg laying hens – reducing IP.FindingsThe mixed logit regression model demonstrated that consumers' WTP for egg attributes associated with beak trimming and IP decreased in the second DCE (12.6% for organic and 2.55% for free-range). For eggshell colour, the analysis revealed a shift from a preference to brown eggs to indifference between eggshell colours.Originality/valueOverall, UK consumers have a preference in higher hen welfare resulting in a decrease in WTP once they are aware of welfare losses in current systems; however, more insights are required in terms of the promotion of white shelled eggs as a means of reducing IP on UK farms.