Ten ISA Brown hens were trained to run an alleyway of 14.4 m in length to obtain a food reward. Each hen was deprived of food for each of 0, 6, 12 and 18 h on four occasions and the times taken to run the alleyway were recorded. The three deprivation periods resulted in greater speeds than the control ( P<0.001), but there was no difference between the speeds for the deprivations. In a second experiment, 15 ISA Brown hens were trained to run the same alleyway for a food reward and then allocated to three groups which received differing numbers of exposures to a feather duster (negative reinforcement) on entry to the goal box. The three groups (0, 1 and 2) were given zero, four or eight exposures, respectively, during a 32 day period. The speeds of Groups 0 and 1 increased linearly during the experiment, but the slopes were not statistically distinguishable. The speeds of Group 2 showed a curvilinear pattern, with no overall change between the start and end of the experiment, but with a decrease in speeds during the middle part of the experiment.