Feather Color Research Articles

The genetic consequences of historic climate change on the contemporary population structure of a widespread temperate North American songbird

Studies of widely distributed species can inform our understanding of how past demographic events tied to historic glaciation and ongoing population genetic processes interact to shape contemporaneous patterns of biodiversity at a continental scale. In this study, we used whole-genome resequencing to investigate the current population structure and genetic signatures of past demographic events in the widespread migratory American goldfinch (Spinus tristis). Phylogenetic relationships inferred from whole mitochondrial genomes were poorly resolved. In contrast, a genome-wide panel of > 4.5 million single nucleotide polymorphisms (SNPs) strongly supported the existence of eastern and western populations separated by western mountain ranges and additional population structuring within the western clade. Demographic modeling estimated that the eastern and western populations diverged approximately one million years ago, and both populations experienced subsequent population bottlenecks during the last glacial period. Species distribution models showed a severe contraction of suitable habitat for the American goldfinch during this period, with predicted discontinuities that are consistent with multiple, isolated glacial refugia that coincide with present-day population structure. Low overall genetic differentiation between the eastern and western populations (FST ∼ 0.01) suggests ongoing gene flow accompanied divergence, and individuals with admixed genomic signatures were sampled along a potential contact zone. Nevertheless, outlier SNPs were identified near genes associated with feather color, song, and migratory behavior and provide strong candidates for further study of the mechanisms underlying reproductive isolation and speciation in birds.

Comparison of the production performance of some phenotypic groups and their crosses of quail birds

The study aimed to determine the production efficiency of local quail phenotypic groups with different feather colors (white, brown, and desert) and their crosses. The results showed significant differences between groups in average body weight at 2, 4, and 6 weeks of age, and in weight gain at 2 and 4 weeks of age in favor of the T6 cross followed by the T5 cross, while T1 recorded the lowest body weight and weight gain compared to other hybrids. Significant differences in feed consumption were observed at 2 and 6 weeks, with no significant differences at 4 weeks. The highest feed consumption was recorded at 6 weeks for hybrids compared to pure groups, and feed conversion efficiency improved at 2 weeks for T5 and T6. At 4 weeks of age, T5, T7, and T6 recorded the best feed conversion efficiency. Positive heterosis in weight at 4 weeks was observed in favor of T5, T6, and T7, while it was negative in T4. Body weight at 6 weeks increased in hybrids T5, T6, and T7 compared to their expected value in the parental groups, and feed conversion efficiency improved at 6 weeks in hybrids T4 and T7. We conclude that heterosis increases the economic value of hybrids, with T6 and T5 being the best hybrids.

Could alternative pathways for carotenoid transformation affect colour production efficiency? A correlative study in wild common crossbills (Loxia curvirostra)

In many vertebrates, dietary yellow carotenoids are enzymatically transformed into 4C-ketocarotenoid pigments, leading to conspicuous red colourations. These colourations may evolve as signals of individual quality under sexual selection. To evolve as signals, they must transmit reliable information benefiting both the receiver and the signaler. Some argue that the reliability of 4C-ketocarotenoid-based colourations is ensured by the tight link between individual quality and mitochondrial metabolism, which is supposedly involved in transforming yellow carotenoids. We studied how a range of carotenoids covary in the feathers and blood plasma of a large number (n > 140) of wild male common crossbills (Loxia curvirostra). Plumage redness was mainly due to 3-hydroxy-echinenone (3HOE). Two other, less abundant, red 4C-ketocarotenoids (astaxanthin and canthaxanthin) could have contributed to feather colour as they are redder pigments. This was demonstrated for astaxanthin but not canthaxanthin, whose feather levels were clearly uncorrelated to colouration. Moreover, moulting crossbills carried more 3HOE and astaxanthin in blood than non-moulting ones, whereas canthaxanthin did not differ. Canthaxanthin and 3HOE can be formed from echinenone, a probable product of dietary β-carotene ketolation. Echinenone could thus be ketolated or hydroxylated to produce canthaxanthin or 3HOE, respectively. In moulting birds, 3HOE blood levels positively correlated to astaxanthin, its product, but negatively to canthaxanthin levels. Redder crossbills also had lower plasma canthaxanthin values. A decrease in hydroxylation relative to ketolation could explain canthaxanthin production. We hypothesize that red colouration could indicate birds' ability to avoid inefficient deviations within the complex enzymatic pathways.

The genetic basis of the kākāpō structural color polymorphism suggests balancing selection by an extinct apex predator.

The information contained in population genomic data can tell us much about the past ecology and evolution of species. We leveraged detailed phenotypic and genomic data of nearly all living kākāpō to understand the evolution of its feather color polymorphism. The kākāpō is an endangered and culturally significant parrot endemic to Aotearoa New Zealand, and the green and olive feather colorations are present at similar frequencies in the population. The presence of such a neatly balanced color polymorphism is remarkable because the entire population currently numbers less than 250 birds, which means it has been exposed to severe genetic drift. We dissected the color phenotype, demonstrating that the two colors differ in their light reflectance patterns due to differential feather structure. We used quantitative genomics methods to identify two genetic variants whose epistatic interaction can fully explain the species' color phenotype. Our genomic forward simulations show that balancing selection might have been pivotal to establish the polymorphism in the ancestrally large population, and to maintain it during population declines that involved a severe bottleneck. We hypothesize that an extinct apex predator was the likely agent of balancing selection, making the color polymorphism in the kākāpō a "ghost of selection past."

Revisiting the hormonal control of sexual dimorphism in chicken feathers

Sexual dimorphism in plumage is widespread among avian species. In chickens, adult females exhibit countershading, characterized by dull-colored round feathers lacking fringe on the saddle, while adult males display vibrant plumage with deeply fringed bright feathers. This dimorphism is estrogen-dependent, and administering estrogen to males transforms their showy plumage into cryptic female-like plumage. Extensive studies have shown that estrogen’s role in female plumage formation requires thyroid hormone; however, the precise mechanisms of their interaction remain unclear. In this study, we investigated the roles of estrogen and thyroid hormone in creating sexual dimorphism in the structure and coloration of saddle feathers by administering each hormone to adult males and observing the resulting changes in regenerated feathers induced by plucking. RT-PCR analysis revealed that the expression of type 3 deiodinase (DIO3), responsible for thyroid hormone inactivation, correlates with fringing. Estrogen suppressed DIO3 and agouti signaling protein (ASIP) expression while stimulating BlSK1, a marker of barbule cells, resulting in female-like feathers with mottled patterns and lacking fringes. Administration of thyroxine (T4) stimulated BlSK1 and proopiomelanocortin (POMC) expression, with no effect on ASIP, leading to the formation of solid black feathers lacking fringes. Triiodothyronine (T3) significantly increased POMC expression in pulp cells in culture. Taken together, these findings suggest that estrogen promotes the formation of solid vanes by suppressing DIO3 expression, while also inducing the formation of mottled patterns through inhibition of ASIP expression and indirect stimulation of melanocortin expression via changes in local T3 concentration. This is the first report describing molecular mechanism underlying hormonal crosstalk in creating sexual dimorphism in feathers.

Genetic diversity, demographic history, and selective signatures of Silkie chicken

BackgroundSilkie is a traditional Chinese chicken breed characterized by its unique combination of specialized morphological traits. While previous studies have focused on the genetic basis of these traits, the overall genomic characteristics of the Silkie breed remain largely unexplored. In this study, we employed whole genome resequencing data to examine the genetic diversity, selective signals and demographic history of the Silkie breed through comparative analyses with seven other Chinese indigenous breeds (IDGBs), a commercial breed, and the wild ancestor Red Jungle Fowl.ResultsIn total, 20.8 million high-quality single nucleotide polymorphisms and 86 large structural variations were obtained. We discovered that Silkie exhibits a relatively high level of inbreeding and is genetically distinct from other IDGBs. Furthermore, our analysis indicated that Silkie has experienced a stronger historical population bottleneck and has a smaller effective population size compared with other IDGBs. We identified 45 putatively selected genes that are enriched in the melanogenesis pathway, which probably is related to the feather color. Among these genes, LMBR1 and PDSS2 have been previously associated with the extra toe and the hookless feathers, respectively. Six of the selected genes (KITLG, GSK3B, SOBP, CTBP1, ELMO2, SNRPN) are known to be associated with neurodevelopment and mental diseases in human, and are possibly related to the distinct behavior of Silkie. We further identified structural variants in Silkie and found previously reported variants linked to hyperpigmentation (END3), muff and beard (HOXB8), and Rose-comb phenotype (MNR2). Additionally, we found a 0.61 Mb inversion overlapping with the GMDS gene, which was previously linked to neurodevelopmental defects in zebrafish and humans. This may also be related to the behavior distinctiveness of Silkie.ConclusionsOur study revealed that Silkie is genetically distinct and relatively highly inbred compared to other IDGB chicken populations, possibly attributed to more prolong population bottlenecks and selective breeding practice. These results enhance our understanding of how domestication and selective breeding have shaped the genome of Silkie. These findings contribute to the broader field of domestication and avian genomics, and have implications for the future conservation and breeding efforts.

Identification of candidate genes associated with primary feathers of tianfu nonghua ducks based on Genome-wide association studies

The primary feathers of ducks have important economic value in the poultry industry. This study quantified the primary feather phenotype of Nonghua ducks, including the primary feathers' length, area, distribution of black spots, and feather symmetry. And genome-wide association analysis was used to screen candidate genes that affect the primary feather traits. The genome-wide association study (GWAS) results identified the genetic region related to feather length (FL) on chromosome 2. Through Linkage disequilibrium (LD) analysis, candidate regions (chr2: 115,246,393-116,501,448 bp) were identified and were further annotated to 5 genes: MRS2, GPLD1, ALDH5A1, KIAA0319, and ATP9B. Secondly, candidate regions related to feather black spots were identified on chromosome 21. Through LD analysis, the candidate regions (chr21: 163,552-2,183,853 bp) were screened and further annotated to 47 genes. Among them, STK4, CCN5, and YWHAB genes were related to melanin-related pathways or pigment deposition, which may be key genes affecting the distribution of black spots on feathers. In addition, we also screened 125 genes on multiple chromosomes that may be related to feather symmetry. Among them, significant SNPs on chromosome 1 were further identified as candidate regions (chr1: 142,118,209-142,223,605 bp) through LD analysis and annotated into 2 genes, TGFBRAP1 and LOC113839965. These results reported the genetic basis of the primary feather from multiple phenotypes, and offered valuable insights into the genetic basis for the growth and development of duck feathers and feather color pattern.

Investigation into the association of FNDC1 and ADAMTS12 gene expression with plumage coloration in Muscovy ducks.

To elucidate the molecular genetic mechanisms underpinning feather color in Muscovy ducks. A cohort of 100 Muscovy ducks was meticulously selected for this research. Follicular tissues from ducks exhibiting black and white plumage served as the experimental samples. From these tissues, RNA and proteins were extracted for further analysis. The RNA underwent reverse transcription polymerase chain reaction amplification, followed by validation through western blot assays. The data revealed a significant upregulation in the expression of FN domain-containing protein 1 (FNDC1) and ADAMTS12 genes in Muscovy ducks with white plumage traits as opposed to those with black plumage traits. Specifically, individuals with pure white plumage demonstrated a markedly elevated expression of the FNDC1 gene in comparison to their pure black counterparts. Conversely, expression levels of the ADAMTS12 gene were found to be reduced in ducks with pure black plumage relative to those with pure white plumage. Notably, the expression patterns of FNDC1 and ADAMTS12 genes exhibited inconsistencies between mRNA and protein levels. This study offers significant insights into the molecular genetic mechanisms underlying feather color variation in Muscovy ducks. FNDC1 and ADAMTS12 could be considered potential targets for genetic manipulation or selective breeding strategies aimed at achieving specific feather color phenotypes in Muscovy ducks.

Characteristics of Several Qualitative Traits of Betet Chickens Raised by Fighting Cock Fans in Central Lombok

The study was conducted to determine the characteristics of betet chickens kept by fighting cock enthusiasts in Central Lombok. Determining the research area was carried out using the cluster sampling method, and sampling of farm was carried out non-randomly using the snowball method, and determining the number of chicken samples for observation was carried out using the accidental sampling method. Observations were made on 74 male Betet and 26 female Betet birds The variables observed were: body posture, feather color, beak color, beak shape, shank color and comb shape. The data obtained were tabulated, the frequency was calculated and analyzed using descriptive analysis. The results of the research found that the feather color of male betet chickens was dominated by bing (black), followed by green, brumbun and the smallest frequency of lurik colors. The feather color of female betet chickens was dominated by the alap color, followed by the kelawu color and the lowest frequency of the berumbun color. The highest frequency of beak color was yellow, followed by black, and the majority was sharply curved. The highest frequency of shank color in male betet chickens was yellow, followed by black and the lowest frequency was white. There were no white shanks found in female betet birds. The highest frequency of the comb shape for male betet chickens was without a comb because it is cut, followed by the single shape, then the lowest frequency was the pea shape. The majority of female betet chickens have a single comb, the rest do not have a comb (the comb does not develop). It was concluded that betet chickens kept by fighting cock enthusiasts

Transcriptome analysis of sexual dimorphism in dorsal down coloration in goslings

BackgroundIn day-old Hungarian white goose goslings, there is a noticeable difference in dorsal down coloration between males and females, with females having darker dorsal plumage and males having lighter plumage. The ability to autosex day-old goslings based on their dorsal down coloration is important for managing them efficiently and planning their nutrition in the poultry industry. The aim of this study was to determine the biological and genetic factors underlying this difference in dorsal down colorationthrough histological analysis, biochemical assays, transcriptomic profiling, and q‒PCR analysis.ResultsTissue analysis and biochemical assays revealed that compared with males, 17-day-old embryos and day-old goslings of female geese exhibited a greater density of melanin-containing feather follicles and a greater melanin concentration in these follicles during development. Both female and male goslings had lower melanin concentrations in their dorsal skin compared to 17-day-old embryos. Transcriptome analysis identified a set of differentially expressed genes (DEGs) (MC1R, TYR, TYRP1, DCT and MITF) associated with melanogenesis pathways that were downregulated or silenced specifically in the dorsal skin of day-old goslings compared to 17-day-old embryos, affecting melanin synthesis in feather follicles. Additionally, two key genes (MC1R and MITF) associated with feather coloration showed differences between males and females, with females having higher expression levels correlated with increased melanin synthesis and darker plumage.ConclusionThe expression of multiple melanogenesis genes determines melanin synthesis in goose feather follicles. The dorsal down coloration of day-old Hungarian white goose goslings shows sexual dimorphism, likely due to differences in the expression of the MC1R and MITF genes between males and females. These results could help us better understand why male and female goslings exhibit different plumage patterns.

House finch leg color changes with infection

In numerous animals, dramatic coloration (e.g. bright red or yellow) often indicates potential fitness, but it is less clear whether subtle coloration in the same individuals (e.g. grey or brown) could also encode such signals. To determine if subtle coloration could indicate health in a bird species with colorful feathers, house finches (Haemorhous mexicanus), we used spectrometry to objectively quantify leg brightness and saturation before, six days after, and 13 days after captive individuals were inoculated with a naturally‐occurring bacterial pathogen, Mycoplasma gallisepticum. We found that legs became less bright six days after infection (near the typical peak of infection), then more bright and less saturated at 13 days (entering the typical recovery period). These results suggest that subtle colors could reliably indicate current infection status, and therefore survival potential.

Identification of Differentially Expressed Genes and microRNAs in the Gray and White Feather Follicles of Shitou Geese.

The Shitou goose, a highly recognized indigenous breed with gray plumage originating from Chaozhou Raoping in Guangdong Province, China, is renowned for being the largest goose species in the country. Notably, during the pure breeding process of Shitou geese, approximately 2% of the offspring in each generation unexpectedly exhibited white plumage. To better understand the mechanisms underlying white plumage color formation in Shitou geese, we conducted a comparative transcriptome analysis between white and gray feather follicles, aiming to identify key genes and microRNAs that potentially regulate white plumage coloration in this unique goose breed. Our results revealed a number of pigmentation genes, encompassing TYR, TYRP1, EDNRB2, MLANA, SOX10, SLC45A2, GPR143, TRPM1, OCA2, ASIP, KIT, and SLC24A5, which were significantly down-regulated in the white feather follicles of Shitou geese. Among these genes, EDNRB2 and KIT emerged as the most promising candidate genes for white plumage coloration in Shitou geese. Additionally, our analysis also uncovered 46 differentially expressed miRNAs. Of these, miR-144-y may play crucial roles in the regulation of feather pigmentation. Furthermore, the expression of novel-m0086-5p, miR-489-y, miR-223-x, miR-7565-z, and miR-3535-z exhibits a significant negative correlation with the expression of pigmentation genes including TYRP1, EDNRB2, MLANA, SOX10, TRPM1, and KIT, suggesting these miRNAs may indirectly regulate the expression of these genes, thereby influencing feather color. Our findings provide valuable insights into the genetic mechanisms underlying white plumage coloration in Shitou geese and contribute to the broader understanding of avian genetics and coloration research.

Weaving feathers of intangible and tangible knowledge: Historical records and human‐bird interactions in the Marquesas Islands

AbstractBirds fulfilled an essential role in ancient Polynesian cultures. They were prized for their bones and colourful feathers, fat, and protein; their cultural importance is further highlighted in Polynesian oral histories. This research investigates the dynamics of human‐bird interactions over time in the Marquesas Archipelago as known from archaeology, oral histories, ethnohistorical records, and museum collections. I analyse the range of avian taxa represented across the various datasets and their cultural uses. The results demonstrate that while some species are represented across one or more datasets, understanding the full extent of species’ cultural roles and use requires more than one thread of analysis, particularly for those taxa with limited or no archaeological signature. Two forms of managed resource use emerge from this study. One is tapu (prohibition of use), and the second is the conservative use of birds as a resource such as restricted feather collecting. Red feathers, represented in museum objects and historical accounts, highlight multiple taxa being used for specific adornment types. The combined analyses provide insights into the loss and persistence of species, as well as aspects of Marquesan cultural management.

Taxonomy of Naumann’s and dusky, red-throated and black-throated thrush complexes (Aves: Turdidae) using morphological and molecular characteristics

Abstract Species are the basic units in evolution and biodiversity and thus a reasonable species classification is essential to assess and protect biodiversity. The classifications of the Naumann’s and dusky thrush complexes and the red-throated and black-throated thrush complexes are not well defined in Turdidae (Aves, Passeriformes). Herein, we use qualitative and quantitative morphological characters and molecular data to review the taxonomy of these thrush complexes. These four thrushes can be distinguished with qualitative features of feather color and striping. These birds cannot be divided by quantitative features, which only work on two groups of thrush complexes. Phylogenetic analysis based on mitochondrial genes indicate that all haplotypes might be divided into two monophyletic clades corresponding to two groups of thrush complexes. According to coalescence-based species delimitation and the gen-morph species concept, these two groups of thrush complexes should be separated as two distinctive species (T. naumanni and T. ruficollis) and including two subspecies per species. This species classification is also supported by gene flow analysis. There is a significant mixing between these two thrush species in the neighbor-joining (NJ) tree and the cluster analysis using a few microsatellite loci. This leads to a pattern of nuclear-mitochondrial discordance between the two birds that can be explained by several potential mechanisms. This study provides the first comprehensive classification of the two thrush complexes on a population level using morphological and molecular characteristics, which will be useful in expanding our understanding of species division among thrush complexes.

Unique feather color characteristics and transcriptome analysis of hair follicles in Liancheng White ducks

Avian feather color is a fascinating trait, and the genetic mechanism of duck plumage formation is still in the preliminary stage. In this study, feather color of Liancheng White ducks was analyzed by determination of melanin content and RNA-seq analysis. In this research, 9 ducks from Mallards (n = 3), Liancheng White (n = 3) and Pekin ducks (n = 3) were used by high performance liquid chromatography (HPLC) and Masson-Fontana staining to reveal the difference of feather melanin content. RNA-seq from 11 hair follicle tissues (1- and 8-wk-old) of Liancheng White ducks (n = 5) and Pekin ducks (n = 7) was used to analyze the candidate genes for the feather melanin synthesis, and Immunofluorescence experiment was used to show the protein expression in 6 black- and white-feathered ducks. Pectorale, skin, liver, fat, brain, heart, kidney, lung, spleen of an 8-wk-old black-feathered Mallard were collected for candidate gene expression. The results showed that the contents of feathers, beak, web melanin in Liancheng White ducks were higher than in Pekin ducks (p < 0.05). Melanin within hair follicles was located in the barb ridge and hair matrix of black feather duck, also we found that TYRP1, TYR, SOX10 genes were differentially expressed between Liancheng White and Pekin ducks (p < 0.05), and these genes were mainly expressed showed in duck skin tissues. This study revealed the unique feather color phenotype of Liancheng White duck shedding light on the transcriptome that underlies it.

Preening correlates with lower feather bacteria abundance but not feather coloration in a lek‐breeding bird

Feathers are structures unique to birds that serve important functions such as flight, thermoregulation, and communication. Bacteria that live on the feathers, particularly ones that can break down keratin, have the potential to damage feathers and disrupt their use in communication. We predicted that birds could behaviorally manage their feather bacterial abundances by preening their feathers. We also predicted that individuals with lower feather bacterial abundances would have brighter and more colorful feathers. To test these predictions, we measured the amount of time individuals in a colony of captive Indian peafowl Pavo cristatus spent preening their feathers. We also collected feathers to determine bacteria abundance on the feather surface and to measure feather coloration. We found that birds had lower feather bacteria levels when they spent more time preening their own feathers, but only in female birds. We also found that bacteria abundances were not correlated with any feather color variables we measured. These results suggest that birds can manage feather bacterial abundances by preening but feather bacteria may not influence feather coloration in this species.

Sexual Dimorphism in Plumage Coloration of Swainson's Hawks (Buteo swainsoni)

ABSTRACT Plumage pigmentation and ornamentation can indicate male phenotypic quality in many avian species and represent traits that are often selected for by females. Melanins are some of the most widespread classes of pigments in the animal kingdom and form the many shades of black and brown feather colors across raptor taxa. Eumelanin is responsible for the darker shades of black and brown, whereas pheomelanin is responsible for the lighter brown shades and rusty red coloration that are found in many buteonine raptors, including Swainson’s Hawks (Buteo swainsoni). Plumage polymorphism has been examined in various raptor species along with morphometric differences as a basis for sexual dimorphism; however, the potential for sexual dimorphism in the form of pheomelanin-pigmented plumage has rarely been discussed. We examined this phenomenon in Swainson’s Hawks by digitally sampling breast feathers from adult museum specimens, and we utilized an imaging toolbox for multispectral image analysis in the open-source ImageJ software to compare red pixel values between sexes. We found a significant difference in normalized red pixel value means between male and female birds, with higher redness values strongly correlating with male individuals, regardless of morph type. This indicates a relationship between pheomelanin pigmentation and sex, illustrating a basis for sexual dimorphism within the species, as well as the potential for sexual selection of redder males.

Cretaceous fossil birds from China

Abstract The explosive radiation of early birds has been well documented by the extraordinary discoveries of the Early Cretaceous fossil birds in China in the past three decades. They have greatly expanded the diversity, disparity and temporal distribution of Mesozoic birds, refining our knowledge of the evolutionary path leading to the characteristic avian body plan, including feathers and powered flight, locomotion and habitat differentiation, diet and digestion, reproduction and development, feather colours and display. In addition, studies on the geological background of the Jehol Biota that produced the majority of the Chinese Cretaceous birds have provided important clues to our understanding of the taphonomy as well as the interaction between deep geological process and biological evolution in northeastern China and East Asia during the Early Cretaceous. Future multidisciplinary studies are expected to advance our knowledge of the palaeogeography of Early Cretaceous birds, what impacted the changes in the diversity of early birds in China, and the roles that early birds played in the Early Cretaceous terrestrial ecosystem.

Sistem Monitoring Pakan dan Air Minum Burung Peliharaan Pemakan Biji Berbasis Internet Of Things

Grain-eating songbirds are often kept as a hobby. These birds have beautiful feather colors and melodious voices. One of the obstacles to maintaining songbirds is that the owner needs more time. The owner is busy working and going out of town, which can cause the bird not to get adequate feed and water, and the owner is also unable to monitor the health condition of the bird. Based on this, a songbird feeding and monitoring system based on IoT was created to monitor the remaining food and drinks in the tank using NodeMCU ESP32 as a system control and data sender to the internet. The system can provide feed and drinking water for pet birds automatically and is connected via the internet to Blynk to send monitoring results. Based on monitoring results, the feed consumption of seed-eating songbirds is 4.74 grams daily, equivalent to 43.14% of the bird's body weight.

Convergent evolution of parrot plumage coloration.

Parrots have remarkable plumage coloration that result in part from a unique ability to produce pigments called psittacofulvins that yield yellow to red feather colors. Little is known about the evolution of psittacofulvin-based pigmentation. Widespread color mutations of captive-bred parrots provide perfect opportunities to study the genetic basis of this trait. An earlier study on blue budgerigars, which do not possess psittacofulvins, reveals the involvement of an uncharacterized polyketide synthase (MuPKS) in yellow psittacofulvin synthesis. The blue phenotype had repeatedly appeared in different parrot species, similar to independent experimental replications allowing the study of convergent evolution and molecular mechanism of psittacofulvin-based pigmentation. Here, we investigated the genetic basis of the blue phenotypes in two species of Agapornis parrots, Fischer's lovebird (A. fischeri) and Yellow-collared lovebird (A. personatus). Using whole-genome data, we identified a single genomic region with size <2 Mb to be strongly associated with the color difference between blue and wild-type (WT) birds in both species. Surprisingly, we discovered that the mutation associated with the blue Agapornis phenotype was identical to the previously described substitution causing the functional change of MuPKS in budgerigars. Together with the evidence of shared blue-associated haplotypes and signatures of a selective sweep in this genomic region in both species, we demonstrated both de novo mutation and interspecific introgression play a role in the evolution of this trait in different Agapornis species. The convergent substitution in the same gene in both lovebirds and budgerigars also indicates a strong evolutionary constraint on psittacofulvin-based coloration.