Faunal Assemblages Research Articles

Contrasting patterns of biodiversity across wetland habitats using single‐time‐point environmental DNA surveys

Abstract Wildlife surveys are central to the conservation and restoration management of wetland habitats; however, the often laborious and costly nature of traditional survey methods can constrain the spatial and temporal extent and replication of survey efforts. Environmental DNA (eDNA) technologies now provide the opportunity to reduce some of these limitations, but applications in temporary and permanent freshwater marshes and meadows (herein referred to as ‘wetland habitats’) remain limited. This study investigates the performance of single‐time‐point eDNA surveys for characterizing fish, amphibian and bird assemblages of wetland habitats in south‐eastern Australia. Comparisons were made between coastal and inland wetlands, between wetlands of varying condition and between eDNA and traditional surveys. Findings show that eDNA surveys can provide comprehensive descriptions of faunal assemblages associated with wetland habitats, with resolution similar to that provided by traditional survey approaches. Analyses also revealed significant differences in patterns of biodiversity between wetland types (coastal vs. inland) but not between wetland habitats varying in condition. Overall, this study confirms that eDNA surveys provide a reliable option for undertaking wetland biodiversity assessments aimed at improving biodiversity data coverage for otherwise data deficient wetlands.

An Aptian sponge-associated decapod crustacean assemblage from Cal Cassanyes (Catalonia, north-east Iberian Peninsula): Taxonomy and palaeoecological implications

A highly diverse decapod crustacean fauna is described from the classic Aptian outcrop of Cal Cassanyes (Castellet i la Gornal) in Catalonia. Although decapod crustaceans are a minor component of the rich invertebrate assemblages at that locality, the just over fifty specimens collected comprise sixteen taxa assignable to the Macrura, Anomura and Brachyura. Of these, the last-named are the most prominent group, comprising eleven species, representing seven families, inclusive of one representative of the Eubrachyura. On the basis of this material, two new genera and five new species are erected, namely Pagurus? garrafensis sp. nov., Garrafosopon gen. nov. angustus (Wright and Collins, 1972) comb. nov., Vectis blesai sp. nov., Mesodromilites prietoi sp. nov., Necrocarcinus mariae sp. nov. and Iberodorippe vinea gen. nov., sp. nov. In addition, Hoploparia sp., an indeterminate axiid and one paguroid, as well as one indeterminate galatheoid are recorded here. Material of Distefania incerta (Bell, 1863), the commonest species at the study site by far, of Goniodromites laevis (Van Straelen, 1940) and of Etyxanthosia fossa (Wright and Collins, 1972) represents the first Aptian and stratigraphically oldest records for these taxa. Added to this list are also Eodromites sp., Paranecrocarcinus? sp. and Pseudonecrocarcinus? sp. The high decapod crustacean diversity at Cal Cassanyes establishes this locality as the richest Aptian assemblage from the Iberian Peninsula, but also one of the richest in the world. The faunal assemblages here are dominated by sponges, and the varied composition of the decapod crustacean faunas can be linked to upwelling currents which provided abundant nutrients. In fact, the establishment of sponge communities played a crucial role in activating the food chain and functioned similarly to a patch reef, creating a relatively sheltered environment that facilitated the colonisation of other invertebrate communities, including decapod crustaceans. The latter occupied various trophic levels as both primary and secondary consumers. Current knowledge of Early Cretaceous decapod crustaceans is scant and hinders a thorough palaeobiogeographical analysis. The present study emphasises the challenges surrounding the identification of endemic taxa, thus impacting our understanding of decapod crustacean palaeobiogeography of the late Early Cretaceous.

Large-scale application of palaeoproteomics (Zooarchaeology by Mass Spectrometry; ZooMS) in two Palaeolithic faunal assemblages from China.

The application of Zooarchaeology by Mass Spectrometry (ZooMS) on Pleistocene sites in Europe and northern Asia has resulted in the discovery of important new hominin fossils and has expanded the range of identified fauna. However, no systematic, large-scale application of ZooMS on Palaeolithic sites in East Asia has been attempted thus far. Here, we analyse 866 morphologically non-diagnostic bones from Jinsitai Cave in northeast China and Yumidong Cave in South China, from archaeological horizons dating to 150-10 ka BP. Bones from both sites revealed a high degree of collagen preservation and potentially time-related deamidation patterns, despite being located in very distinct environmental settings. At Jinsitai, we identified 31 camel bones, five of which were radiocarbon dated to 37-20 ka BP. All dated specimens correspond to colder periods of Marine Isotope Stages 3 and 2. We regard the presence of camels at Jinsitai as evidence of wild camels being a megafauna taxon targeted, most likely by early modern humans, during their expansion across northeast Asia. This large-scale application of ZooMS in China highlights the potential of the method for furthering our knowledge of the palaeoanthropological and zooarchaeological records of East Asia.

Tryps-IN: A streamlined palaeoproteomics workflow enables ZooMS analysis of 10,000-year-old petrous bones from Jordan rift-valley

Poor preservation of collagen in dry and/or arid environments has hindered the application of Zooarchaeology by mass spectrometry (ZooMS) analysis in many regions of the world. As a result, many zooarchaeological investigations have relied exclusively on morphological assessment of fragmentary remains due to the inadequate preservation of biomolecules. The climatic conditions of Southwest Asia include extreme temperature fluctuations unconducive to preservation of proteins and DNA. We performed zooarchaeological analysis of remains from the 10,000-year-old site of Shkārat Msaied in Jordan and sub-sampled twenty-eight petrous bones, the hardest bone in the mammalian skeleton, for species identification by ZooMS. Using an unconventional and simplified extraction protocol we call Tryps-IN, in which digestion was performed without removal of the demineralising EDTA, we taxonomically identified several fragments, outperforming an established ZooMS workflow. A subset of identifications was subsequently confirmed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) protein sequencing. The new methodology presented here opens the possibility of further bioarchaeological investigation of other fragmentary faunal assemblages within this region of archaeological significance.

Fossil vertebrates from the Cerro Fortaleza Formation (Upper Cretaceous), Santa Cruz Province, Argentina

The Cerro Fortaleza Formation is a stratigraphic unit that crops out near the Cerro Fortaleza hill, northwestern Santa Cruz province, Argentina. This unit has been well-known since the end of the XIX century, and because of the finding of abundant dinosaur bones, it was named as the “Capas con dinosaurios” (=Dinosaur Beds). Intensive work since the end of the 1990's resulted in the finding of diverse dinosaur species and other vertebrates. The fossil assemblage recovered includes lamniform sharks, lepisosteiforms, the dipnoan Atlantoceratodus iheringi, chelid turtles, peirosaurid crocodyliforms, mosasaur teeth, and diverse dinosaur remains. Dinosaurs include a partial tail of a small-sized titanosauriform, and another, identified as Dreadnoughtus, shows derived procoelous caudals. Isolated sauropod teeth indicate the presence of a derived lithostrotian and the enigmatic Clasmodosaurus spatula Ameghino, 1899. Among theropods, abelisaurid and megaraptorids are reported. A partial ulna, manual claw and metatarsal belonging to the holotype of the enigmatic theropod Austrocheirus isasii are also described. The morphology of the new elements does not comfortably fit with abelisaurid or megaraptorid theropods, and the affinities of Austrocheirus among theropods still remain elusive. The fossil vertebrate content of the Cerro Fortaleza Formation indicates close similarities with the faunistic assemblage previously reported from the Mata Amarilla Formation (Cenomanian-Turonian), and strong differences are present with the overlying Chorrillo Formation (Maastrichtian). This may indicate that some kind of change among terrestrial vertebrates occurred at the Campanian/Maastrichtian boundary in the Austral Patagonia Basin.

The use of chimpanzee-modified faunal assemblages to investigate early hominin carnivory.

Chimpanzees regularly hunt and consume prey smaller than themselves. It seems therefore likely that early hominins also consumed small vertebrate meat before they started using and producing stone tools. Research has focused on cut marks and large ungulates, but there is a small body of work that has investigated the range of bone modifications produced on small prey by chimpanzee mastication that, by analogy, can be used to identify carnivory in pre-stone tool hominins. Here, we review these works along with behavioral observations and other neo-taphonomic research. Despite some equifinality with bone modifications produced by baboons and the fact that prey species used in experiments seldom are similar to the natural prey of chimpanzees, we suggest that traces of chimpanzee mastication are sufficiently distinct from those of other predators that they can be used to investigate mastication of vertebrate prey by early hominins.

Too good to go? Neanderthal subsistence strategies at Prado Vargas Cave (Burgos, Spain)

Understanding the relationship between Neanderthal groups and their environment and they it is essential to comprehending their ways of life. In this article, we use both zooarchaeology and taphonomy to study the fauna assemblages found in Level 4 of Prado Vargas Cave (Cornejo, Burgos, Spain). The results point to a site in which the main accumulating agent was Neanderthal groups, who transported small- and medium-sized animal carcasses—with deer as the dominant taxon—according to their general utility to systematically and intensely exploit the major muscle bundles, bone marrow, skin, and tendons. According to dental microwear analysis, reiterated and prolonged occupations occurred in diverse moments, suggesting that the site was used most often as a long-term campsite. We detected a minimal amount of carnivore activity, who accessed the cave during periods of human absence and modified some of the remains left by the Neanderthals. Together, this information indicates that Prado Vargas is a key site to understand the dynamics of the Neanderthals in the linking area between the Castillan Plateau and the Cantabrian Range.

Historical changes in bird communities at Quitobaquito Springs, Arizona and impacts of climate, spring flow, and shift from Indigenous to federal-agency management

For nearly a century, bird communities at the oasis of Quitobaquito Springs in the Sonoran Desert have attracted desert ecologists. This spring-fed oasis is among the most biologically and culturally significant sites in arid southwestern North America, but has experienced marked changes in management and is threatened by climate change, border, and other development. We assembled data on birds at Quitobaquito across 83 years (1939–2022) and evaluated the patterns and potential drivers of changes in communities. We found evidence of marked community shifts that included apparent loss and replacement of species dependent on riparian and wetland vegetation, mud flats, agricultural fields, and human settlements, to species that use deeper more open-water environments, generalists, and desertscrub. Strong associations between community composition and shift from Indigenous to federal-agency management, temperature, and spring flow suggest important drivers of change, but associations with precipitation and border development were weaker. Such patterns match those for bird communities at broader scales in nearby and other aridlands and indicate impacts of increasing aridification. Our work represents the first analysis of changes in faunal assemblages at Quitobaquito that quantitatively assesses potential drivers of change, and provides insights for management and conservation.

First direct evidence of lion hunting and the early use of a lion pelt by Neanderthals

During the Upper Paleolithic, lions become an important theme in Paleolithic art and are more frequent in anthropogenic faunal assemblages. However, the relationship between hominins and lions in earlier periods is poorly known and primarily interpreted as interspecies competition. Here we present new evidence for Neanderthal-cave lion interactions during the Middle Paleolithic. We report new evidence of hunting lesions on the 48,000 years old cave lion skeleton found at Siegsdorf (Germany) that attest to the earliest direct instance of a large predator kill in human history. A comparative analysis of a partial puncture to a rib suggests that the fatal stab was delivered with a wooden thrusting spear. We also present the discovery of distal lion phalanges of at least 190,000 years old from Einhornhöhle (Germany), representing the earliest example of the use of cave lion skin by Neanderthals in Central Europe. Our study provides novel evidence on a new dimension of Neanderthal behavioral complexity.

First results of the biostratigraphy and geochronology of the classic Nihewan Fauna, China

The Nihewan Basin is infilled by a relatively complete Pliocene to Pleistocene sequence, rich mammalian fossils and containing many Palaeolithic artefacts, implying that it is a unique location for Quaternary multidisciplinary study. Fossil excavations in these deposits have been carried out for nearly a century. The systematic study of the fossils by Teilhard de Chardin and Piveteau in 1930 laid the foundations for the classic Nihewan Faunal assemblage, which has been widely followed as a standard for the Early Pleistocene biochronology in eastern Asia. With the availability of magnetostratigraphic analyses on several sections in the Nihewan basin, a comprehensive geochronological study is timely pending. Here we report the results of a comprehensive survey of the Xiashagou section, where the classic Nihewan Fauna was discovered. By synthesising the lithostratigraphical, biostratigraphical, and magnetostratigraphical information, together with measurements taken by the authors, the total thickness of the Nihewan Formation in the Xiashagou section being 116 m. Furthermore, the geochronological study demonstrates that the classic Nihewan Fauna was originally recovered from at least 6 fossil horizons, spanning from ∼2.4 Ma to ∼1.8 Ma, i.e., the early Early Pleistocene. The new evidence from the Xiashagou section also suggests several cycles of fluctuations in the biodiversity and population of the classic Nihewan Fauna during the early Pleistocene. The preliminary correlation of these cycles matches the short periods of glaciation in the early Pleistocene.

Exploring changing occupation dynamics at the lakeside cave site Matja Kuru 2, Timor-Leste

The cave site known as Matja Kuru 2 (MK2) in Timor-Leste was first occupied ∼40 kya. Of the caves investigated thus far in Timor-Leste MK2 is unique in being located proximal to a large freshwater lake, Ira Lalaro, providing the opportunity to examine changing occupation dynamics in a lakeside environment from the Late Pleistocene through to the late Holocene. We present the analysis of the Matja Kuru 2 assemblage including the stone, ochre, shell and bone artefacts, and the vertebrate and invertebrate remains. We discuss the challenges posed by cave deposits in tropical Island South East Asia which often preserve little in the way of identifiable stratigraphy. Such caves also present challenges in terms of disentangling what components of the faunal assemblage were deposited as meal refuse by humans, as opposed to by other predators or as a result of natural deaths. We suggest a method for assisting with this taphonomic conundrum.

Magnetostratigraphy of the Collepardo Late Pliocene faunal assemblage (Early Villafranchian Triversa Faunal Unit), Ernici Mts., Central Italy

Magnetostratigraphy of the Collepardo Late Pliocene faunal assemblage (Early Villafranchian Triversa Faunal Unit), Ernici Mts., Central Italy

In front of the retreating ice-sheet: Fauna complex of central-western Poland in MIS 3–2 (Krosinko site)

A new locality in Central Europe (Krosinko, Poland) yields a rich fossil material of great taxonomical variety. Its location in the Warsaw–Berlin ice-marginal valley, where it forms a 1-m horizon at a the depth of 6–8 m, is suited to a taphonomic analysis aimed at reconstructing the paleoevents and depositional history of the late Pleistocene (MIS 3–2).Our analysis revealed that the material accumulated as the result of high-energy fluvial transport. This transport disturbed the original bone associations, as indicated by the lack of articulation, low refitting, and bone surface modification. During water movement, the bones were polished, abraded, pitted, and fragmented. Prior to that, however, taphonomic signatures were left behind by predators, humans, and plant roots. This is the first time that unquestionable burning marks have been found on woolly rhino remains from Poland.The secondary context of the faunal assemblage was confirmed by neodymium isotope studies of the faunal fragments and sediment. Given the results of sedimentological analysis from previous studies, these sediments can be associated with a high-energy braided river. The remains were moved along with paleobotanical material and flints in a westerly direction; this is suggested by the direction of water flows in the valley and the river waters supplying it.The deposition occurred within a single geologic event around 26 ka BP, as indicated by the youngest radiocarbon date of the remains, or ca. 19 ka BP, considering the geochronological constraints for the local LGM ice margin position in central and eastern Poland ∼22–18 ka, and the specified accumulation time of the sediments in which they were found. Secondary mineralization of manganese and iron on the bones was the final taphonomical process that impacted the remains.The combined taphonomic, palaeozoological, isotopic and geological results show for the first time for a Polish paleontological site how data integration improves interpretation and highlights the advantages of a multiproxy approach.

Branching Lithophyllum Coralline Algae: Dominant Reef Builders on Herbivory-Depressed Tropical Reefs after High Coral Mortality

A unique shift in benthic community composition, where scleractinian corals are replaced by coralline algae, has been observed on coral reefs in Guam in the western Pacific. Guam’s reefs have been subjected to intense fishing pressure and impaired water quality for decades. Since 2013, heat stress has emerged as an additional major threat to the island’s coral reefs. After a severe coral bleaching and mortality event in 2017, branching coralline algae of the genus Lithophyllum rapidly overgrew dead coral skeletons of the ecosystem engineer Acropora abrotanoides and have since become major components of forereef communities over a broad depth range. By now, the persistence of increased Lithophyllum cover meets the temporal criterium of phase shifts, but accurate estimates on the degree of dominance over appropriate spatial scales are lacking due to the absence of reliable baseline data. The ecological impacts of coral reef transitions towards increased coralline cover are unclear. Whereas carbonate budgets and reef growth could remain positive in the long term, the downstream effect of changes in structural complexity, (micro)habitat diversity, and benthic community composition on ecological processes and reef-associated faunal assemblages is unknown.

Eocene Kohat Formation suitability as an aggregate from Kohat Range, Sub-Himalayas, Pakistan, based on outcrop, petrographic and geotechnical properties

This paper evaluates the physicochemical and mechanical characteristics of the Limestone of the Kohat Formation. The Eocene Kohat limestone is generally exposed in the Kohat, Hangu, and Bannu districts. Kohat Formation limestone is well exposed throughout the Northern Kohat fold and thrust belt at Togh Bala village. It is cream to grey, hard, compact, well-bedded and fossiliferous. Kohat Formation has been identified into two lithofacies which are from base to top calcareous shale lithofacies with large benthic foraminifera and limestone lithofacies. The faunal assemblage indicates that the Kohat Formation was deposited in an inner to middle shelf environment. The petrographic analyses of samples of the Kohat Formation depict that it principally consists of calcite (60-90%) with a small to trace assemblage of dolomite (0-3%), ungraded quartz (0-1.5%), opaque minerals (0-1.2%) and fossils (8-40%). Aggregate physical properties like water absorption (0.88%), specific gravity (2.69), soundness (2.55%), Los Angeles abrasion value (23.06%), aggregate crushing value (11.56%), aggregate impact value (12.68%), flaky index (9.90%), elongation index (12.76%) and coating and stripping values (96.27%) conform to available standards. Our results indicate that Kohat Limestone is safe in terms of Alkali Carbonate Reactivity (ACR) and Alkali-Silica Reactivity (ASR). The facies, thin section study, geochemical, and geotechnical understanding of Kohat Formation indicate that these aggregate deposits are potential aggregate sources for ballast (railway), road, building material, concrete, mortar and other mega projects for connecting Asia and Europe.

Palaeoenvironmental and chronological context of hominin occupations of the Armenian Highlands during MIS 3: Evidence from Ararat-1 cave

Archaeological and palaeoenvironmental evidence from the Armenian Highlands and wider southern Caucasus region emphasises the significance of Marine Oxygen Isotope Stage 3 (c. 57–29 ka) as a crucial period for understanding hominin behaviours amidst environmental fluctuations. Ararat-1 cave, situated in the Ararat Depression, Republic of Armenia, presents potential for resolving emerging key debates regarding hominin land use adaptations during this interval, due to its well-preserved lithic artefacts and faunal assemblages. We present the first results of combined sedimentological, geochronological (luminescence and radiocarbon), archaeological and palaeoecological (macrofauna, microfauna and microcharcoal) study of the Ararat-1 sequence. We demonstrate sediment accumulation occurred between 52 and 35 ka and was caused by a combination of aeolian activity, cave rockfall and water action. Whilst the upper strata of the Ararat-1 sequence experienced post-depositional disturbance due to faunal and anthropogenic processes, the lower strata remain relatively undisturbed. We suggest that during a stable period within MIS 3, Ararat-1 was inhabited by Middle Palaeolithic hominins amidst a mosaic of semi-arid shrub, grassland, and temperate woodland ecosystems. These hominins utilised local and distant toolstone raw materials, indicating their ability to adapt to diverse ecological and elevation gradients. Through comparison of Ararat-1 with other sequences in the region, we highlight the spatial variability of MIS 3 environments and its on hominin land use adaptations. This demonstrates the importance of the Armenian Highlands for understanding regional MP settlement dynamics during a critical period of hominin dispersals and evolution.

Taphonomic approaches to distinguish chewing damage from knapping marks in Palaeolithic faunal assemblages

The imprint of human actions on mammal remains from archaeological sites is often fragmented and attenuated due to post-mortem processes, which add to the challenge of distinguishing human from natural modifications in faunal assemblages. Identifying minimally-worked bone tools poses a particular challenge when they are mixed with bones that have been modified by other agents. Bones, antlers and teeth used as hammers or pressure-flakers in flint-knapping can be particularly difficult to identify because knapping damage resembles carnivore chewing marks. This paper presents a methodological approach to establish diagnostic criteria for identifying whether bones from archaeological sites were modified by chewing or knapping, using observations at macroscopic and microscopic levels. We applied these criteria to case studies from the Upper Palaeolithic (Magdalenian) site of Gough’s Cave (UK). Analysis of Magdalenian stone tools shows that blades were detached with a soft hammer, yet organic (soft) knapping tools appear to be scarce in Magdalenian contexts. We propose that the difficulty in identifying knapping damage on bones that have undergone only a short period of use, combined with the macroscopic similarity of these marks with natural modifications (e.g., carnivore chewing), are significant factors contributing to the rarity of minimally-modified knapping tools in archaeological contexts.

Animal Consumption at Hospital de San Martín (Gran Canaria): First Zooarchaeological Analysis in the Modern Era of the Canary Islands (Fifteenth-Eighteenth Centuries CE)

AbstractThe Canary Islands were initially colonized around 200 CE by North African Berber populations who brought with them domestic plants and animals. These communities remained isolated until the arrival of Europeans in the Late Middle Ages which triggered the conquest of the archipelago. Its geostrategic location in the framework of Atlantic expansion facilitated the arrival of people, knowledge, goods, plants, and animals which served to shape the new Canarian society. This study explores the dietary practices of this period through the zooarchaeological analysis of the faunal remains recovered at the Hospital de San Martín (fifteenth to the eighteenth centuries), Las Palmas (Gran Canaria, Spain). Our results suggest that caprines were the main taxa consumed. This follows both the pre-colonial Indigenous tradition and, according to historical sources, the subsequent European period. The faunal assemblage recovered also reveals the first directly radiocarbon-dated cases in the Canary Islands of three hitherto unknown species: cattle, chicken, and rabbit. Moreover, the results evidence a progressive increase throughout the Modern Era of chicken and rabbit, and that translocating these new species to the archipelago during the European expansion led to a diversification of meat resources.

CARNIVORA FROM THE EARLY PLEISTOCENE OF GRĂUNCEANU (OLTEŢ RIVER VALLEY, DACIAN BASIN, ROMANIA)

The Grăunceanu site in the Olteţ River Valley has yielded a rich carnivoran assemblage including at least 10, possibly 11 species: Vulpes alopecoides, Nyctereutes megamastoides, Ursus etruscus, Meles thorali, Lutraeximia sp., Pliocrocuta perrieri, Lynx issiodorensis, Puma pardoides, Megantereon cultridens, Homotherium latidens and possibly Pachycrocuta brevirostris. The faunal assemblage is compared with approximately coeval sites Europe and western Asia. This comparative analysis shows that the Grăunceanu assemblage shows the greatest similarities with sites to the west such as Senèze and, particularly, Saint-Vallier, rather than more easternly ones such as Liventsovka and Dmanisi. The relative abundance of the taxa at Grăunceanu was compared to that of Saint-Vallier and broad similarities were found, except for the absence of some cursorially adapted taxa present at Saint-Vallier but absent from Grăunceanu. The absence at Grăunceanu of taxa with a reconstructed cursorial hunting strategy, such as Chasmaporthetes and Acinonyx, may suggest less open habitat at Grăunceanu than indicated in previous paleoenvironmental reconstructions.

Return to 1616: Multispecies Fauna Reconstruction Requires Thinking Outside the Box.

Conservation translocations have become increasingly popular for 'rewilding' areas that have lost their native fauna. These multispecies translocations are complex and need to consider the requirements of each individual species as well as the influence of likely interactions among them. The Dirk Hartog Island National Park Ecological Restoration Project, Return to 1616, aspires to restore ecological function to Western Australia's largest island. Since 2012, pest animals have been eradicated, and conservation translocations of seven fauna species have been undertaken, with a further six planned. Here, we present a synthesis of the innovative approaches undertaken in restoring the former faunal assemblage of Dirk Hartog Island and the key learnings gathered as the project has progressed.