Fatty Carboxylic Acids Research Articles

Synthesis and characterization of antioxidant-enriched Moringa oil-based edible oleogel

Abstract This study aims to formulate and optimize Moringa oleifera (Moringa) oil oleogels using pectin (PC) and chitosan (CS) as gelling agents. These include monogelator oleogels, utilizing either PC or CS as a single gelling agent, and binary gelator oleogels, incorporating a combination of both PC and CS. Among the binary gelator oleogel compositions, the most stable oleogel OPCCS2 was further studied with the addition of antioxidants. The important antioxidant compounds of gallic acid equivalents (GAEs)/Moringa antioxidant extracts (MAEs) were quantified by the use of various assays. The oil-binding capacity (OBC) of the most stable oleogel MCPC1.5% was 99.94 ± 0.05. The lower peroxide value of antioxidant-rich oleogels at 1.5% concentration of GAEs (4.34 ± 0.025) and MAEs (4.32 ± 0.03) suggested its richness of phenols to retard the lipid peroxidation of oil. The opaque appearances of the formulations were studied via polarizing light microscopy. The molecular interaction study through FTIR analysis revealed the hydrogen bond interactions between the carboxyl groups of fatty acids and hydroxyl groups of polysaccharide chains. The differential scanning calorimeter analysis further confirmed the presence of strong interactions between polysaccharide chains and the oil phase. These findings indicate that the optimized oleogel formulations have the potential for imminent advances by exhibiting improved texture, biocompatibility, enhanced OBC, and stability.

Pemafibrate and other triglyceride-lowering therapies to reduce risk of cardiovascular and metabolic disease.

Although high triglycerides are consistently associated with elevated risk of cardiovascular disease (CVD), therapies that reduce triglyceride levels have inconsistently translated into reduced CVD risk. To date, three clinical trials have tested triglyceride-lowering therapies in patients with hypertriglyceridemia (HTG) and elevated risk of incident/recurrent CVD. In REDUCE-IT (Reduction of Cardiovascular Events with Icosapent Ethyl-Intervention Trial), assignment to IPE, a highly purified eicosapentanoic acid (EPA), resulted in a 25% reduction in nonfatal myocardial infarction), nonfatal stroke, cardiovascular death, coronary revascularization and hospitalization for unstable angina. By contrast, the combination of EPA and docosahexanoic acid (DHA) carboxylic fatty acids used in the STRENGTH trial (Statin Residual Risk With Epanova in High Cardiovascular Risk Patients With Hypertriglyceridemia) failed to reduce CVD risk. Most recently, PROMINENT (Pemafibrate to Reduce Cardiovascular Outcomes by Reducing Triglycerides in Patients with Diabetes) also failed to demonstrate reduction in CVD events despite use of a potent triglyceride-lowering, fibric-acid derivative. However, improvement in HTG-associated metabolic complications (e.g. nonalcoholic fatty liver disease) was observed with pemafibrate as well as with another potent triglyceride-lowering therapy (i.e. pegozafermin). Moreover, trials are underway evaluating whether the most fatal metabolic complication of HTG, pancreatitis, may be reduced with highly potent triglyceride-lowering therapies (e.g. apolipoprotein C3 inhibitors). Taken together, HTG is associated with increased risk of CVD and attendant adverse metabolic sequalae. To this end, a potentially promising and evidence-based landscape is emerging for treating a clinical phenotype that in the past has been insufficiently addressed.

NiCo/SiO2 nanospheres for efficient synergetic decarboxylation of fatty acids and upgrading of municipal sludge HTL bio-crude to biofuels

Municipal sludge (MS) and derived HTL (hydrothermal liquefaction) bio-crude contains a large quantity of organics, including fatty acids, which may be a potential resource for producing biofuels via catalytic treatment. Herein, decarboxylation of fatty acids is chosen as the model reaction to screen a series of nickel-based bimetallic catalysts supported on homemade SiO2 nanospheres to investigate the synergetic effect. NiCo/SiO2 turned out with the best performance compared to NiCu-, NiZn-, NiMo- and NiFe/SiO2. An 83% conversion of stearic acid and an 84% selectivity of heptadecane via hydrothermal decarboxylation were achieved at 280 °C under 1 MPa H2. XPS results revealed the electron transfer from Co to Ni, leading to the formation of Niδ-, which is more accessible to bond with Cδ+ in the carboxyl group of fatty acids, thus facilitating the decarboxylation reaction. Compared to commercial SiO2, our homemade SiO2 showed a stronger carbon accumulation resistance, indicating a medium specific surface area was essential. Both the synergetic effect of NiCo bimetal and the carbon accumulation resistance of homemade SiO2 nanospheres contributed to the efficient hydrothermal decarboxylation. The NiCo/SiO2 catalyst was further applied for upgrading of MS HTL bio-crude to biofuels, and the theoretical higher heating value (HHV) was significantly improved from 34.4 to 37.2 MJ/kg, suggesting the potential application of converting MS bio-crude to biofuels.

Seasonal expressions of COX-1, COX-2 and EP4 in the scent glands of muskrats (Ondatra zibethicus).

Prostaglandins (PGs) serve as signaling molecules that regulate various physiological processes, including inflammation, immune response, blood clotting and reproduction. The aim of this study was to investigate the immunolocalizations and expression patterns of prostaglandin-E2 (PGE2), cyclooxygenase (COX)-1 and COX-2, as well as its receptor subtypes 4 (EP4) in the scent glands of muskrats (Ondatra zibethicus) during the breeding and non-breeding periods. There were significant seasonal differences in the scent glandular mass, with higher values in the breeding season and relatively low in the non-breeding season. PGE2, EP4, COX-1 and COX-2 have been immunolocalized in the scent glandular and epithelial cells in both breeding and non-breeding seasons, whereas no immunostaining was observed in the interstitial cells. The protein and mRNA expression levels of EP4, COX-1 and COX-2 were higher in the scent glands of the breeding season than those of the non-breeding season. The mean mRNA levels of EP4, COX-1 and COX-2 were positively correlated with the scent glandular weights. The circulating follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and PGE2, as well as scent glandular PGE2 and dihydrotestosterone (DHT) concentrations were also significantly higher in the breeding season. Additionally, the transcriptomic study in the scent glands identified that differentially expressed genes might be related to fatty carboxylic monocarboxylic acid, steroidogenic-related pathway and prostanoid metabolic process. These findings suggested that prostaglandin-E2 might play an essential autocrine or paracrine role in regulating seasonal changes in the scent glandular functions of the muskrats.

Aqueous Phase from Hydrothermal Liquefaction: Composition and Toxicity Assessment

The main obstacle to the widespread use of hydrothermal liquefaction (HTL) for waste and wet biomass recycling is the formation of a significant amount of highly polluted wastewaters. This paper presents an analysis of the chemical composition and toxicity of aqueous phase from the HTL (HTL-AP) of primary and secondary sludge. It was shown that HTL-AP has a high level of organic pollution (total organic carbon (TOC) = 4.2–9.6 g/dm3, chemical oxygen demand (COD) = 7.9–14.0 g/dm3, BOD5 = 6.0–8.1 g/dm3) and high biological toxicity for traditional test organisms (so that dilution ratio, ensuring the death of no more than 50% of organisms (DR50), varied within 64.7–142.2 and 44.9–81.7 for Artemia salina and Paramecium caudatum, respectively). An analysis of HTL-AP composition with NMR-spectroscopy method allowed us to establish that the share of carbon in aliphatic chains was 34.05–41.82% and the content of carbon in carboxyl groups and aromatic rings was 26.42–34.44%. As a result, we can conclude that the main HTL-AP components are fatty carboxylic acids and their derivatives, aromatic carboxylic acids. The content of aldehydes, ketones, and lignin is less than 8%. Biological treatment of HTL-AP in a lab-scale aerobic reactor turned out to be successful, so average COD reduction was 67–95%. Sludge from an industrial waste water treatment plant (petrochemical sector) with a microorganism concentration of 2.7 g/dm3 was used as inoculum. HTP-AP was diluted 1:10 with tap water. The duration of the process was 18 h.

Cancer cell growth suppressibility of ω-3 fatty acid whose carboxy group converted to ester group

Recently, ω-3 fatty acids have been revealed to having cancer cell growth suppressibility. It is necessary to analyze the mechanism of cancer cell growth suppressibility and to impart selective cancer cell accumulation when creating anticancer drugs based on ω-3 fatty acids. Therefore, it is necessarily essential to introduce a luminescent molecule or a molecule which have a drug delivery function into ω-3 fatty acids, and the position of introduction is the ω-3 fatty acids’ carboxyl group. On the other hand, whether the ω-3 fatty acids’ cancer cell growth suppressibility is maintained when the ω-3 fatty acids’ carboxyl groups are converted to other structures, such as ester groups, is unclear. In this work, a derivative was synthesized wherein the α-linolenic acid carboxyl group, one of the ω-3 fatty acids, was converted to an ester group and evaluated the cancer cell growth suppressibility, as well as the amount of cancer cell uptake. As a result, it was suggested that the ester group derivatives presented the same functionality as α-linolenic acid, and the ω-3 fatty acid carboxyl group is a flexible functional group, which can be structurally modified in terms of functionality to cancer cells.

Energy-Resolved Ion Mobility Spectrometry: Composite Breakdown Curves for Distinguishing Isomeric Product Ions.

Identification of lipopeptides (LpAA) synthesized from bacteria involves the study of structural characterization. Twenty LpAA have been characterized using commercial tandem high-resolution mass spectrometers in negative electrospray, employing nonresonant excitation in "RF only" collision cells and generally behave identically. However, [LpAA-H]- (AA = Asp or Glu) shows surprising fragmentation pathways, yielding a complementary fatty acid carboxylate and dehydrated amino acid fragment anions. In this study, the dissociation mechanisms of [C12Glu-H]- were determinate using energy-resolved mass spectrometry (ERMS). Product ion breakdown profiles are, generally, unimodal with full width at half-maximum (fwhm) increasing as product ion m/z ratios decrease, except for the two product ions of interest (fatty acid carboxylate and dehydrated glutamate) characterized by broad and composite profiles. Such behavior was already shown for other ions using a custom-built guided ion beam mass spectrometer. In this study, we investigate the meaning of these particular profiles from an ERMS breakdown, using fragmentation mechanisms depending on the collision energy. ERMS on line with ion mobility spectrometry (IMS), here called ER-IMS, provides a way to probe such questions. Broad or composite profiles imply that the corresponding product ions may be generated by two (or more) pathways, resulting in common or isomeric product ion structures. ER-IMS analysis indicates that the fatty acid carboxylate product ion is produced with a common structure through different pathways, while dehydrated glutamate has two isomeric forms depending on the mechanism involved. Drift time values correlate with the calculated collision cross section that confirms the product ion structures and fragmentation mechanisms.

Growth of Prebiotically Plausible Fatty Acid Vesicles Proceeds in the Presence of Prebiotic Amino Acids, Dipeptides, Sugars, and Nucleic Acid Components.

Fatty acid vesicles may have played a role in the origin of life as a major structural component of protocells, with the potential for encapsulation of genetic materials. Vesicles that grew and divided more rapidly than other vesicles could have had a selective advantage. Fatty acid vesicles grow by incorporating additional fatty acids from micelles, and certain prebiotic molecules (e.g., sugars, nucleobases, and amino acids) can bind to fatty acid vesicles and stabilize them. Here, we investigated whether the presence of a variety of biomolecules affects the overall growth of vesicles composed of decanoic acid, a prebiotically plausible fatty acid, upon micelle addition. We tested 31 molecules, including 15 dipeptides, 7 amino acids, 6 nucleobases or nucleosides, and 3 sugars. We find that the initial radius and final radius of vesicles are largely unaffected by the presence of the additional compounds. However, three dipeptides enhanced the initial rates of growth compared to control vesicles with no small molecules added; another three dipeptides decreased the initial rates of growth. We conclude that vesicles can indeed grow in the presence of a wide range of molecules likely to have been involved in the origin of life. These results imply that vesicles would have been able to grow in complex and heterogeneous chemical environments. We find that the molecules that enhance the initial growth rate tend to have hydrophobic groups (e.g., leucine), which may interact with the lipid membrane to affect growth rate; furthermore, the molecules that cause the largest decrease in initial growth rate are dipeptides containing a serine residue, which contains a hydroxyl group that could potentially hydrogen-bond with the fatty acid carboxylate groups.

Применение катионного красителя пиронина G (Y) для количественного экстракционно-фотометрического определения высших карбоновых кислот в рыбе

The relevance of this study is due to the need to develop methods for the extraction and quantitative determination of higher carboxylic acids in fish. In this work, we propose a simple, inexpensive, and highly sensitive extraction-photometric technique for the selective determination of the total content of fatty acids. This technique is selective and is based on the quantitative extraction into the organic phase of ionic associates of the cationic dye pyronin G (Y) with highly hydrophobic carboxylic acids and seems promising for the quantitative determination of the fatty carboxylic acids in fish. Considerable attention is paid to the sample preparation, which is the most important stage of analysis, since the work is carried out with a natural sample that has a complex composition. The technique has been tested on real objects, also using this technique, a directly proportional dependence of the concentration of higher carboxylic acids in fish on the storage time has been obtained. It has been established that the heptane/iso-propanol system is the most effective for the extraction of higher carboxylic acids. The limit of detection of higher carboxylic acids by the extraction-photometric method using the cationic dye pyronin G(Y) in fish is 4,4 10-7 M. The results obtained allow us to state that the developed method can be used to determine the freshness of fish.

Growth Kinetics of Zinc Sulphide Nanocrystals Synthesized by Colloidal Thermolysis of Fatty Acid Carboxylates

Zinc sulphide (ZnS) is an important semiconductor with widespread electronic and catalytic applications. The growth kinetics of ZnS nanocrystals synthesized by the thermal decomposition of zinc ricinoleate carboxylates in an oleylamine:dodecanethiol (1 : 1) solvent mixture isreported. Crystalline sphalerite ZnS nanocrystals with quantum dot sizes of 2.3–5.3 nm were obtained at temperatures higher than 240°C. The p-XRD patterns showed a clear relationship between the crystallite sizes and the peak broadness at a temperature range of 250–300°C. The optical bandgap energies of the ZnS nanocrystals reduced from 4.27 eV to 3.73 eV as the time of reaction increased from 20 to 60 min at 250°C. The activation energy for the growth kinetics of the ZnS nanocrystals was determined to be 36.24 kJ/mol which compares closely to those reported in the literature for aqueous systems.

Metabolite profiling of Bruguiera cylindrica reveals presence of potential bioactive compounds

Bruguiera cylindrica parts are commonly used in Chinese and Indian traditional medicine to treat diarrhea, fever, and many ailments. The present study aims non targeted analysis of key secondary metabolites of B. cylindrica by gas chromatography mass spectrometry (GC-MS) and ultra-high performance liquid chromatography hybrid quadrupole-Exactive-Orbitrap high resolution mass spectrometry (UHPLC-Q-Exactive Orbitrap HRMS). GC-MS and UHPLC-Q-Exactive Orbitrap HRMS were utilized for metabolic profiling of ethyl acetate extract of B. cylindrica leaves. Key metabolites in the extract were identified and predicted based on chemical similarity using online databases such as ChemSpider and mzCloud. Thirty-six compounds belonging to different classes of secondary metabolites viz. flavonoids, fatty acids, fatty acid amides, carboxylic acids, and alkaloids were identified in the extract. Pentacyclic triterpenes like betulin, ursolic acid and a tropine, an alkaloid with potential pharmacological and therapeutic activities such as anticancer properties, neuromuscular blockers and antioxidants, were also identified. This study combined GC-MS and UHPLC-Q-Exactive Orbitrap HRMS with available online database for effective and rapid identification of bioactive metabolites in the ethyl acetate extract of mangrove without individual standard application. This is the first report on the HRMS based secondary metabolic profiling of B. cylindrica, with comprehensive map of its biologically important metabolites.

In-Situ Implant Formulation of Laurate and Myristate Prodrugs of Dolutegravir for Ultra-Long Delivery

The focus of present work was to synthesize prodrugs of dolutegravir (DTG) for ultra-long delivery purpose. The prodrug was synthesized by esterification of hydroxyl group with carboxyl group of fatty acid (lauric or myristic acid). The prodrugs were characterized by differential scanning calorimetry, X-ray powder diffraction, nuclear magnetic resonance, Fourier transformed infrared, near infrared-chemical imaging, pH-solubility, partition coefficient, and stability (solid and liquid). Stability studies were performed by exposing the powder drugs to 40°C/75% RH for three months and buffer solutions at room temperature for 72 h. The prodrugs and drug were formulated into in-situ implant using biodegradable polymer. Thermal, spectral, and diffractometric data indicated formation of new chemical and solid forms. Formation of prodrugs resulted in lowering of melting point of DTG from 191.1°C to 163.7 and 140.7°C for DTG-Laurate and DTG-Myristate prodrugs, respectively. A decrease in solubility of 18.2-115.9 and 124.5-1594.9 folds was observed for DTG-Laurate and DTG-Myristate, respectively compared to DTG. Similarly, the prodrugs were highly lipophilic compared to DTG. Solid-state and pH-stability profiles of DTG and prodrugs were comparable. Implant formulation released 60.1% in 77 days compared to 95.6% in 35 days in the case of DTG-Myristate and DTG, respectively. In summary, combining prodrug and drug delivery approaches can be utilized for delivering drug for ultra-long period.

SYNTHESIS OF METAL CARBOXYLATES FROM ALMOND SEED OIL (TERMINALIA CATAPPA

TThe less exploited seed oil of almond was investigated for its potential utilization as a raw material for the synthesis of metal carboxylates. The oil was extracted from the almond seeds and a yield of 42% was obtained. The oil was characterized and used to synthesize the carboxylates of barium, calcium, cobalt, copper, iron, magnesium, nickel and zinc. The functional groups of fatty acid carboxylates produced were confirmed by FTIR. The characteristic colours of the carboxylates were white (barium, calcium, magnesium and zinc carboxylates), ash (Cobalt carboxylate), mint green (copper carboxylate), brown (iron carboxylate), and lemon green (nickel carboxylate). The pH of the solutions of the metal carboxylates varies from 6.6 to 10 with no free alkaline. The foam stability test revealed a length of 1.3 cm for barium carboxylate and 0.3 cm for magnesium carboxylate while other carboxylates did not foam.

Identification of bacterial lipo-amino acids: origin of regenerated fatty acid carboxylate from dissociation of lipo-glutamate anion

The identification of bacterial metabolites produced by the microbiota is a key point to understand its role in human health. Among them, lipo-amino acids (LpAA), which are able to cross the epithelial barrier and to act on the host, are poorly identified. Structural elucidation of few of them was performed by high-resolution tandem mass spectrometry based on electrospray combined with selective ion dissociations reach by collision-induced dissociation (CID). The negative ions were used for their advantages of yielding only few fragment ions sufficient to specify each part of LpAA with sensitivity. To find specific processes that help structural assignment, the negative ion dissociations have been scrutinized for an LpAA: the N-palmitoyl acyl group linked to glutamic acid (C16Glu). The singular behavior of [C16Glu-H]¯ towards CID showed tenth product ions, eight were described by expected fragment ions. In contrast, instead of the expected product ions due to CONH-CH bond cleavage, an abundant complementary dehydrated glutamic acid and fatty acid anion pair were observed. Specific to glutamic moiety, they were formed by a stepwise dissociation via molecular isomerization through ion–dipole formation prior to dissociation. This complex dissociated by partner splitting either directly or after inter-partner proton transfer. By this pathway, surprising regeneration of deprotonated fatty acid takes place. Such regeneration is comparable to that occurred from dissociation to peptides containing acid amino-acid. Modeling allow to confirm the proposed mechanisms explaining the unexpected behavior of this glutamate conjugate.

Surface-Modified and Unmodified Calcite: Effects of Water and Saturated Aqueous Octanoic Acid Droplets on Stability and Saturated Fatty Acid Layer Organization.

A profound understanding of the properties of unmodified and saturated fatty acid-modified calcite surfaces is essential for elucidating their resistance and stability in the presence of water droplets. Additional insights can be obtained by also studying the effects of carboxylic acid-saturated aqueous solutions. We elucidate surface wettability, structure, and nanomechanical properties beneath and at the edge of a deposited droplet after its evaporation. When calcite was coated by a highly packed monolayer of stearic acid, a hydrophilic region was found at the three-phase contact line. In atomic force microscopy mapping, this region is characterized by low adhesion and a topographical hillock. The surface that previously was covered by the droplet demonstrated a patchy structure of about 6 nm height, implying stearic acid reorganization into a patchy bilayer-like structure. Our data suggest that during droplet reverse dispensing and droplet evaporation, pinning of the three-phase contact line leads to the transport of dissolved fatty carboxylic acid and possibly calcium bicarbonate Ca(HCO3)2 molecules to the contact line boundary. Compared to the surface of intrinsically hydrophobic materials, such as polystyrene, the changes in contact angle and base diameter during droplet evaporation on stearic acid-modified calcite are strikingly different. This difference is due to stearic acid reorganization on the surface and transport to the water–air interface of the droplet. An effect of the evaporating droplet is also observed on unmodified calcite due to dissolution and recrystallization of the calcite surface in the presence of water. In the case where a water droplet saturated with octanoic acid is used instead of water, the stearic acid-coated calcite remains considerably more stable. Our findings are discussed in terms of the coffee-ring effect.

Compounds isolated from hexane fraction of Alternanthera brasiliensis show synergistic activity against methicillin resistant Staphylococcus aureus

Abstract Methicillin resistant Staphylococcus aureus (MRSA) has been classified as a “serious threat” by the centre for Disease Control, USA. Alternanthera brasiliensis plant, usually found on wasteland, belongs to the family Amaranthaceae. It is traditionally used for wound healing and has shown antimicrobial effect. Yet, this plant has not been fully explored for its antibacterial activity. Hence, this study evaluated isolated compounds from this plant for its activity against MRSA infections. The leaves extracts and fractions were prepared and concentrated in vacuo using a rotatory evaporator. Isolated compounds were obtained through vacuum liquid chromatographic (VLC) techniques and structurally elucidated with various spectroscopic techniques. Anti-MRSA assay of the fraction and compounds were evaluated by agar-well diffusion and broth-dilution methods while checkerboard assay was used to determine the fractional inhibitory concentration index (FICi). The Gas Chromatography-Mass Spectrometry (GCMS) and High Performance Liquid Chromatography (HPLC) analysis revealed fatty acid and carboxylic acid components like hexadecanoic acid, bis (2-ethylhexyl) phthalate and Fettsäure. The compounds AbHD1 and AbHD5 were identified as hexadecanoic acid and di (ethylhexyl) phthalate. Anti-MRSA assay shows that A. brasiliensis hexane fraction (AbHF) and the compounds had zones of inhibitions (Zi) ranging from 7.3 ± 0.5 to 17.5 ± 0.5 mm with minimum inhibitory concentrations (MIC) between 1.22 × 10−5 – 2.5 mg/mL. Synergistic effects were observed between AbHF and erythromycin, AbHF and ampicillin and AbHF and ciprofloxacin with FICi 0.208–0.375 in K1St4 strain while amoxicillin revealed antagonistic effects against M91 strain (4.67). Similarly, hexadecanoic acid and di (ethylhexyl) phthalate showed synergistic behaviour only with ampicillin against K1St4 while the rest were antagonistic. The study revealed that hexadecanoic acid and di (ethylhexyl) phthalate isolated from A. brasiliensis showed synergistic activity in variations against MRSA isolate and strains.

ГИДРОФОБИЗАЦИЯ ТОНКОДИСПЕРСНОГО МЕЛА

Natural fine chalk (calcium carbonate) is widely used functional filler for composite materials used in many industries. The development of in-depth studies of the physicochemical properties of hydrophobic chalk contributes to the expansion of its application. The analysis of scientific and technical literature has shown that at the moment there are no studies of the parameters of the porous structure and textural parameters of natural chalk of various granulometric composition, especially superficially treated (hydrophobized) with fatty carboxylic acids. Carboxylic acids of C17 to C20 fractions are practically applied for hydrophobization of calcium carbonate that is conditioned by presence of these acids with enough long hydrocarbon chains and reactive carboxyl group. The treatment of chalk with hydrophobizing additive gives it the following advantages over untreated chalk: prevention of moisture accumulation, absence of caking and improvement of technological properties (flowability, compatibility with polymer and others). The paper presents the results of chemical and mineralogical analyses of natural chalk from the Sheinsky deposit, data on the differential distribution of particles, the degree of hydrophobicity, and the study of the textural characteristics of natural and hydrophobized fine chalk. The influence of the grinding method on the granulometric composition is evaluated and the cumulative effect of the grinding method and hydrophobization on the texture characteristics of chalk is revealed. It is established that the surface treatment of chalk particles with fatty acids (C18 content not less than 65 wt%) affects the process of nitrogen gas adsorption

Effectiveness of isolated compound from Aloe barbadensis Miller and its formulated ointment against bacteria and fungi

Ethnomedicinally, the family Liliaceae is prominent in controlling skin, wound and fungal infections. A carboxylic fatty acid derivative has been isolated from Aloe vera leaves and its structure was elucidated on the basis of NMR and FT-IR analysis. The compound was formulated as ointment with the aid of polymer based delivery agent. The ointment and the uncontaminated fatty acid have been scrutinized aimed at their anti-infectious potential. Individually, the pure compound and the formulated ointment exhibited growth inhibitory activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans and Talaromyces flavus. Minimum inhibitory concentration (MIC) values were found to be appreciably low, ranging between 0.30 and 2.50 mg/ml. The formulated ointment displayed highly significant inhibitory activities against all six pathogens compared to the unformulated compound. The MIC values of the ointment ranged between 0.08 and 1.25 mg/g. The in vitro antibiotic activity studies discovered that, both the pure compound and the formulated ointment showed potency against the selected multi-resistant microorganisms tested in the current work. These findings suggest that the isolated carboxylic acid derivative may be beneficial in the discovery of antibiotics highly potent against drug-resistant pathogens. Furthermore, it may add to the improvement of preservatives in the food industry. Lastly, it could be considered as new source of natural antibiotics in the pharmaceutical industry.

Design, synthesis, antibacterial activity and toxicity of novel quaternary ammonium compounds based on pyridoxine and fatty acids.

A diverse series of 43 novel "soft antimicrobials" based on quaternary ammonium pyridoxine derivatives which include six-membered acetals and ketals of pyridoxine bound via cleavable linker moieties (amide, ester) with a fragment of fatty carboxylic acid was designed. Nine compounds exhibited invitro promising antibacterial activity against Gram-positive and Gram-negative bacterial strains with MIC values comparable with reference antiseptics miramistin, benzalkonium chloride and chlorohexidine. On various clinical isolates, the lead compounds 6i and 12a exhibited antibacterial activity comparable with that of benzalkonium chloride while higher than that of miramistin. Moreover, 6i and 12a were able to kill bacteria embedded into the matrix of mono- and dual species biofilms. The treatment of bacterial cells by either 6i and 12a lead to fast depolarization of the membrane suggesting that the membrane is an apparent molecular target of compounds. 6i and 12a were non mutagenic neither in SOS-chromotest nor in Ames test and non-toxic invivo at acute oral (LD50>2000mg/kg) and cutaneous administration (LD50>2500mg/kg) on mice. Taken together, our data allow suggesting described active compounds as promising starting point for the new antibacterial agents development.

ХРОМАТО-МАСС–СПЕКТРОМЕТРИЯ ХЛОРОФОРМНОГО ЭКСТРАКТА ТЫСЯЧЕЛИСТНИКА ОБЫКНОВЕННОГО (ACHILLED MILLEFOLIUM L., СЕМЕЙСТВО АСТРОВЫЕ — ASTERACEAE) (сообщение III)

The purpose of the study is to detail the information about the peculiarities of the chemical composition and structural organization of different groups of compounds of organic matter, yarrow, revealing the influence of nature of the extractant on the qualitative composition and quantitative content of extractable compounds in the obtained chloroform extract, and identifying new pharmacological actions of the latter, the extension of the range of compounds already known in the literature on herbal medicine. Materials and methods of research. The chemical composition of the chloroform extract was studied by chromatography-mass spectrometry under the following conditions. The chemical composition of the ethanol extract of yarrow was used by chromatography-mass spectrometry under the following conditions. GC-2010 gas chromatograph connected to a triple quadrupole mass spectrometer GCMS-TQ-8030 running GCMS Solution 4.11 software. Identification and quantitative determination of the content of compounds were performed under the following chromatography conditions: sample input with flow division (1:10), ZB-5MS column (30m × 0.25 mm × 0.25 mm), injector temperature 280 °C, carrier gas-helium, gas velocity through the column 29 ml/min. Conclusion. For the first time, the chemical composition of chloroform extract, a product of sequential exhaustive extraction of yarrow by chromatography — mass spectrometry, was studied, which allows identifying 94 individual compounds for which mass spectra and structural formulas were obtained, the quantitative content was determined, and the structural and group composition of the extract was calculated. The basis of the composition of the chloroform extract is determined by hydrocarbons enriched with arenes, alkenes and alkynes, esters formed mainly by oxalic and benzoic acids, polyunsaturated fatty carboxylic acids, steroid compounds, alcohols, nitrogen-containing structures that include individual fragments of natural alkaloids, as well as a variety of organosilicon compounds that determine the main directions of pharmacological action of various yarrow preparations, including chloroform extract.