Compounds isolated from hexane fraction of Alternanthera brasiliensis show synergistic activity against methicillin resistant Staphylococcus aureus

Abstract

Abstract Methicillin resistant Staphylococcus aureus (MRSA) has been classified as a “serious threat” by the centre for Disease Control, USA. Alternanthera brasiliensis plant, usually found on wasteland, belongs to the family Amaranthaceae. It is traditionally used for wound healing and has shown antimicrobial effect. Yet, this plant has not been fully explored for its antibacterial activity. Hence, this study evaluated isolated compounds from this plant for its activity against MRSA infections. The leaves extracts and fractions were prepared and concentrated in vacuo using a rotatory evaporator. Isolated compounds were obtained through vacuum liquid chromatographic (VLC) techniques and structurally elucidated with various spectroscopic techniques. Anti-MRSA assay of the fraction and compounds were evaluated by agar-well diffusion and broth-dilution methods while checkerboard assay was used to determine the fractional inhibitory concentration index (FICi). The Gas Chromatography-Mass Spectrometry (GCMS) and High Performance Liquid Chromatography (HPLC) analysis revealed fatty acid and carboxylic acid components like hexadecanoic acid, bis (2-ethylhexyl) phthalate and Fettsäure. The compounds AbHD1 and AbHD5 were identified as hexadecanoic acid and di (ethylhexyl) phthalate. Anti-MRSA assay shows that A. brasiliensis hexane fraction (AbHF) and the compounds had zones of inhibitions (Zi) ranging from 7.3 ± 0.5 to 17.5 ± 0.5 mm with minimum inhibitory concentrations (MIC) between 1.22 × 10−5 – 2.5 mg/mL. Synergistic effects were observed between AbHF and erythromycin, AbHF and ampicillin and AbHF and ciprofloxacin with FICi 0.208–0.375 in K1St4 strain while amoxicillin revealed antagonistic effects against M91 strain (4.67). Similarly, hexadecanoic acid and di (ethylhexyl) phthalate showed synergistic behaviour only with ampicillin against K1St4 while the rest were antagonistic. The study revealed that hexadecanoic acid and di (ethylhexyl) phthalate isolated from A. brasiliensis showed synergistic activity in variations against MRSA isolate and strains.