The biodegradation of anionic synthetic detergents with a linear alkyl chain has been followed in laboratory activated sludge units. It was found by comparing the concentration of the methylene blue active substance (MBAS) remaining in the effluent, with the appertaining values for total organic carbon (TOC) and dichromate value (DV), respectively, that the degradation of the detergent molecule did not coincide with the MBAS degradation. Generally, these indirect methods gave a lower rate of degradation. Thus, the presence of intermediates, which are not accounted for in the MBAS-analysis, is postulated. A higher proportion of intermediates are formed with alkylbenzene sulfonates (LAS) than with alkyl sulfates, alkyl sulfonates and fatty alcohol ether sulfates, intermediates are produced to a greater extent as the detergent concentration increases. Therefore LAS-detergents should be described as of medium biodegradability for practical purposes.