Fatty Acid Profile Research Articles

Composition of raw and roasted runner, Spanish and Valencia market type peanuts

Spanish, Valencia, and runner are three peanut market-types which have distinct physical and chemical profiles that can affect flavor and industry uses. Market-type and roast treatment (raw/roasted) were evaluated for physical and chemical differences between raw (skin removed) and dry roasted seeds. Samples were analyzed for seed weight, moisture content, water activity, total oil, fatty acid profiles, tocopherols, protein, sugars, and free amino acids. Runner-types had the largest seed weight relative to Spanish and Valencia seeds. Roasting significantly affected moisture content and water activity. Total oil ranged from 49.66 % to 55.63 % with the runner samples having the highest content. Oleic, palmitic, and linoleic fatty acids were the most abundant in the samples and significantly differed between market-types with ranges between 54.74 % and 82.65 %, 5.82–9.04 %, and 2.63–3.06 % respectively. Samples were discriminated by market-type for alpha, beta, and gamma tocopherols. The Valencia-types were highest in protein. Sucrose was the major sugar quantified, but market-type and roasting had no effect on the content. Glucose and fructose levels significantly decreased after roasting. Several free amino acids were differentiated by market-type, but glutamic acid and phenylalanine were the most abundant in all the samples. Roasting demonstrated a significant effect on several free amino acids.

Determination of the Impact of Extruded Soybean Press Cake on Rearing and Health Indices of Piglets

The study was conducted to evaluate the alternative to imported soybean meal—extruded soybean press cake—in feeding weaning pigs. The experiment was carried out with 20 35-day-old weaned pigs weighing about 8.8 kg, divided into 2 groups (10 pigs in each) differing in the main protein source: group I—control, solvent-extraction soybean meal, group II— extruded soybean press cake. The feed mixtures were iso-protein and iso-energetic. After 35 days of feeding, the pigs were slaughtered. The samples of blood, intestinal tissue and digesta, meat, and back fat were collected for analyses. Replacement of soybean meal with extruded soybean press cake in the weaners’ diet had no statistically significant effect on growth performance and feed utilization. Histomorphometry analysis of the intestines did not indicate any harmful changes. The soybean press cake did not affect the sum of volatile fatty acids in the large intestine, but the ammonia concentration was lower in the distal colon (p ≤ 0.05). It was found that the extruded soybean press cake in the feed significantly affected the fatty acid profile (p ≤ 0.05) of meat and back fat, increasing the amount of PUFAs; however, no negative impact on the durability of meat and back fat during storage was noted. In conclusion, the study confirmed the possibility of completely replacing solvent-extraction soybean meal with extruded soybean press cake in the feeding of weaned pigs.

Analysis of Nutritional Composition of the Pearl Oyster Pinctada radiata as a New Mediterranean “Bioresource” for Human Consumption

Protein intake inadequacy has been considered to be one of the major nutritional problems worldwide for many years and it appears that this issue will continue to increase sharply in the coming decades. This deficiency can be partly overcome by the effective use of protein-rich bioresources such as mollusks. In the present study, the oyster Pinctada radiata, collected from the Aegean Sea, is fully nutritionally characterized as a new non-indigenous bioresource concerning mainly its protein, carbohydrate and fat composition during the different seasons of the year. The results showed that the protein content of the pearl oyster is at satisfactory levels, with its maximum value in winter and minimum in summer. On the contrary, its fat and carbohydrates are at low levels, with their maximum values in the summer period. Regarding the profile of fatty acids, polyunsaturated fats are in the highest proportion, which is very encouraging, as a diet rich in this kind of fat is desired. The entire nutritional profile of the studied oyster demonstrates its high nutritional value and supports its possible use as a “new” seafood source for human consumption in the Mediterranean.

Evaluating the Potential of Marine Algae as Sustainable Ingredients in Poultry Feed

Algae are a complex source of crucial nutrients, including proteins, minerals, vitamins, and carbohydrates. The use of marine algae, specifically Sargassum species, Gracilaria species, and Spirulina species, as feed ingredients in poultry feed was examined in the current research. This study’s aims were to carry out lipid profiling, proximate analysis, and heavy metal toxicity testing, as well as to evaluate the presence of hazardous hydrocarbons in the algae and investigate the effect of the fatty acid profile of Sargassum species on broilers. The proximate analysis was conducted on the algae types containing considerable quantities of fats and proteins and a sizable quantity of omega-3 fatty acids. This research assessed the productive effectiveness of broilers with 2.5%, 6%, and 10% algal additions in their diet. Fatty acid profiling of Sargassum species was performed at 1% and 2% levels in the bursa, fat pads, heart, liver, spleen, thymus, thighs, and breast. The findings showed no significant differences in the development performance or feed utilization efficiency of broilers with 2.5%, 6%, and 10% algae supplementation in their diet. The results of fatty acid profiling showed changes in the heart, liver, and spleen at 1% and 2% supplementations levels. However, this research concludes that if algae partially replaces the imported feed proteins used in animal feed, then these can achieve maximum beneficial effects on poultry health and performance. Algae are sustainable, easy to produce, and cost-effective sources of nutrients and contain numerous bioactive compounds, which will contribute to sustainability and food safety.

The Influence of Crossbreeding on the Composition of Protein and Fat Fractions in Milk: A Comparison Between Purebred Polish Holstein Friesian and Polish Holstein Friesian × Swedish Red Cows.

In this study, the differences in protein and fat bioactive components between the milk from purebred Polish Holstein Friesian (PHF) cows and PHF cows crossbred with Swedish Red (SRB) were investigated. The objective was to assess the impact of genetic variation on the nutritional quality of their milk. This study was conducted at the Warsaw University of Life Sciences' (WULS) experimental dairy farm in Warsaw, Poland, and involved 60 primiparous cows divided into two groups: 30 PHF×SRB crossbred cows and 30 purebred PHF cows. All cows were housed in a free-stall system with an average lactation yield exceeding 10,000 kg/lactation. The milk composition analyses included total protein, casein, whey protein, fatty acid profiles, and vitamin content. Milk from the PHF×SRB hybrids showed a significantly greater total protein content (3.53%) compared to that from the purebred PHF cows (3.28%). The casein content was higher in the hybrids' milk (2.90%) than the purebreds' milk (2.78%), while the whey protein levels were lower in the purebred milk (0.50%) than in the hybrid milk (0.63%). The hybrids exhibited higher concentrations of certain saturated fatty acids in their milk, while the purebreds' milk contained greater amounts of beneficial unsaturated fatty acids and fat-soluble vitamins-E, D, and K. These results indicate that genetic selection through crossbreeding can enhance the nutritional quality of milk. The differences observed in protein, fatty-acid, and vitamin content underscore the role of the genotype in milk composition, suggesting that breeding strategies can optimize dairy products' health benefits.

Effect of dietary supplementation of rubber seed kernel pellet on feed utilization, rumen fermentation, fatty acid profiles and health status in swamp buffalo.

Rubber seed kernel is a by-product of rubber cultivation and are high in oil and protein. This study was conducted to evaluate the effect of supplementing rubber seed kernel pellet (RUSKEP) on feed intake, nutrient digestibility, rumen fermentation, rumen fatty acid profiles, blood parameters, and immune response in swamp buffalo. Four male swamp buffalo with an initial body weight (BW) of 254 ± 10 kg and 26 months of age were used in this research. The experimental design was a 4  4 Latin squared design with RUSKEP supplementation at 0, 4, 6, and 8% of dry matter intake (DMI). Animals were fed concentrate at 1.0% BW, while rice straw was fed ad libitum. Supplementation with RUSKEP did not change DMI or nutrient digestibility (p>0.05), while ether extract (EE) digestibility increased cubically with the addition of RUSKEP (p<0.01). The ruminal pH, ammonia-nitrogen (NH3-N), and the proportion of acetate (C2) were similar among treatments (p>0.05). The proportion of propionate (C3) increased linearly (p≤0.04), leading to a decrease in the acetate to propionate ratio (C2:C3) (p≤0.04) with the addition of RUSKEP. Furthermore, the butyrate (C4) proportion decreased linearly with RUSKEP supplementation (p=0.03). The addition of RUSKEP did not affect on linoleic acid (LA; C18:2 cis-9,12 + trans-9,12), or α-linolenic acid (ALA; C18:3 cis-9,12,15) (p>0.05). With RUSKEP supplementation, the stearic acid (C18:0) content increased quadratically (p<0.01). The increasing level of RUSKEP was higher in cholesterol and eosinophils (p≤0.03). The immune function (IgA, IgM, and IgG) was similar among treatments (p>0.05). Supplementing RUSKEP with up to 8% of DMI could improve rumen fermentation efficiency without affecting feed utilization, rumen PUFA profile, or immune response in swamp buffalo.

Microbial community acclimation during anaerobic digestion of high-oil food waste

Anaerobic digestion is one of the most promising options for the disposal of biodegradable food waste. However, the relatively high content of oil in food waste inhibits the conversion efficiency of anaerobic digestion because of the accumulation of long-chain fatty acids (LCFAs). In this study, activated anaerobic sludge was acclimated to accommodate high-oil conditions. The methane yield of high-oil food waste digested by the acclimated sludge increased by 24.9% compared to that digested by the raw sludge. To determine the internal changes in the acclimated sludge, the shifts in the microbial communities during the acclimation period were investigated via high-throughput sequencing (HTS) based on the 16 S rRNA gene. The results indicated that Bacteroidetes, Firmicutes, Chloroflexi and Proteobacteria were the dominant bacteria at the phylum level. The acclimation time allows some functional bacterial taxa to proliferate, such as Clostridium and Longilinea, which are able to degrade LCFAs and turn them into small organic molecules that also have nutrient value for other bacteria. Among the archaeal communities, the hydrogenotrophic methanogen Methanobacterium nearly supplanted the acetotrophic methanogen Methanosaeta. The time profiles of volatile fatty acids (VFAs) and pH during this period provided additional evidence for the success of the acclimation. This study demonstrated the effectiveness of acclimation and the dynamic of microbial communities, which further contributed to the management and resource utilization of high-oil food waste.

Genetic Loci Mining and Candidate Gene Analysis for Determining Fatty Acid Composition in Rice

Fatty acid composition and its proportions are critical to the nutritional value and storage quality of rice (Oryza sativa L.) as the third major nutrient component in this staple food. This study involved crossing an indica rice variety, Huazhan (HZ), as the male parent, with a japonica variety, Nekken2, as the female parent, to produce the F1 generation. Subsequently, a population of 120 recombinant inbred lines (RILs) was developed through multiple generations of self-breeding. By utilizing a high-density molecular genetic linkage map and phenotypic data of four fatty acid components, we identified a total of 14 quantitative trait loci (QTLs) related to fatty acid composition across chromosomes 1, 3, 4, 6, 8, and 9. These included two QTLs for C14 content, three for C16:0 content, six for C18:1 content, and three for C18:2 content. Notably, the QTL qCOPT4.2 exhibited a high LOD score of 5.22. Within QTL intervals, genes such as OsACX3 and SLG affecting grain length were identified. The expression of candidate genes within these intervals was assessed and further analyzed by using quantitative real-time PCR. Genes such as LOC_Os01g15000, LOC_Os04g47120, LOC_Os04g49194, LOC_Os06g22080, LOC_Os06g23870, LOC_Os06g24704, LOC_Os06g30780, LOC_Os08g44840, and LOC_Os09g36860 were found to regulate fatty acid synthesis or metabolic pathways, potentially enhancing fatty acid content in rice. These QTLs are indispensable for breeding rice varieties with improved fatty acid profiles, offering new genetic resources for enhancing the nutritional and storage qualities of rice.

Effect of different levels of turmeric (Curcuma longa) on productivity in broiler chickens

This research was conducted to evaluate the effect of different levels of turmeric powder on the performance, blood parameters, immunity, intestinal morphology, carcass characteristics, cecum microbial flora, fatty acid profile and sensory evaluation of breast meat of broiler chickens. A completely randomized design was used including four levels of turmeric powder (250, 500, 750 and 1000 mg/kg diet) and a control treatment (without turmeric), in 5 replications. The growth performance was measured periodically and at the end of the 42-day period, three chickens from each experimental unit were killed for carcass examination. In order to check blood parameters at the end of the course, blood samples were taken from three chickens from each experimental unit. To check the immunity of broiler chickens, the weight percentage of immune glands was also evaluated. To measure the morphology of the cecum, one chicken from each experimental unit was killed to check the desired traits. The results of the experiment showed that different levels of turmeric had a significant effect on performance, carcass characteristics, blood parameters, immunity, intestinal morphology, cecal microbial flora, fatty acid profile, and the sensory evaluation of breast meat (P&lt;0.05). The results of the experiment showed body weight, feed intake and feed conversion ratio (FCR) at the end of the period were significant in the treatments that received turmeric powder (P&lt;0.05). Survival percentage, produced body weight per square meter, and final profit was also significant (P&lt;0.05). Relative weight of abdominal fat, villus height and villus height/ crypt depth of the small intestine were significant in the treatments receiving turmeric powder (P&lt;0.05). By adding turmeric powder to the diet of broiler chickens, the microbial population of the cecum also became significant (P&lt;0.05). The concentration levels of uric acid, total cholesterol, glucose, HDL, albumin and liver enzymes alkaline phosphatase and ALT were significant in the birdsreceiving turmeric powder (P&lt;0.05). Turmeric powder in the diet of broilers also had a significant effect on chondroitinic acid, linoleic acid and total polyathenoids (P&lt;0.05). Turmeric powder was significant on the lactobacilli microbial population (P&lt;0.05). The results of this experiment showed that fat content, juicyness, crispyness, chewability, elasticity, mouthfeel and general acceptance were significantly improved (P&lt;0.05). In this research, the treatment receiving 500 mg of turmeric powder per kg had the greatest effect on the measured characteristics of broiler chickens. It can be concluded that the addition of the powder of these medicinal and aromatic plants to the diet of broiler chickens has beneficial effects on the carcass characteristics and meat quality. Keywords: fatty acid, blood plasma, abdominal cavity, triglyceride, feed conversion ratio, Curcuma longa

The effect of high levels of Glycyrrhiza glabra powder on performance, carcass characteristics, blood parameters, immunity, intestinal microbial flora, intestinal morphology and breast muscle fatty acid profile in broilers

In order to investigate the effect of licorice medicinal plant on performance, carcass characteristics, fatty acid profile of breast muscle, intestinal microbial flora, blood constitutes and immunity of broiler chickens, an experiment using a completely randomized design with 3 treatments, 4 replications, 10 chicks per repetition and a total of 120 pieces of one-day-old male broiler chickens of commercial strain Ross 308 were performed for 42 days. Experimental treatments included three different levels of licorice (0, 300 and 400 mg/kg feed). Data was analyzed using SAS statistical software and averages were compared at the 5% probability level with Duncan's multiple range test. The results showed that although variation in feed consumption, weight gain and feed conversion ratio was not significant in any of the rearing periods (P≥0.05), the highest feed consumption, weight gain and the best feed conversion ratio was observed in the final period of chickens were fed with Diet containing 400mg/kg licorice. Also, according to the data, the best European index was related to this treatment. In addition, the use of two levels of licorice had no significant effect on carcass characteristics or intestinal microbial flora; however, the highest increase in body weight and the lowest number of Lactobacillus acidophilus bacteria were observed at the higher level of licorice. However, these high levels did not lead to a decrease in abdominal fat. According to the results obtained, different levels of licorice led to a significant reduction of harmful fats such as cholesterol, triglycerides, VLDL and LDL (P&lt;0.05). Furthermore, although the level of HDL was not significant (P≥ 0.05), its highest amount was seen at high levels of licorice. In addition, the lowest amount of total protein, albumin and globulin was observed at the level of 400 mg/kg of licorice. According to these results, although the amount of glucose was lowest its level was not significant (P≥0.05), Also, the use of this same level of licorice had no significant effect on the function of the humoral immune system in response to antigen injection (SRBC Antibody against sheep red blood cell) or antibody titer against Newcastle virus and influenza (P≥0.05). Nevertheless, according to the data, the highest antibody titer against Newcastle virus and SRBC antigen was associated with this treatment. These results also showed that high levels of licorice led to an increase in the number of Bifidobacterium. Villi width and crypt depth decreased at the high dose of licorice, but the ratio of villi length to crypt depth increased. In addition, the use of different levels of licorice could not reduce the ratio of saturated to unsaturated fatty acids compared to the control group. Therefore, based on the results of this research, it is recommended to use the level of 400 mg/kg of licorice to supplement the diet of broilers of Ross 308 strain.

Luteibacter sahnii sp. nov., A Novel Yellow-Colored Xanthomonadin Pigment Producing Probiotic Bacterium from Healthy Rice Seed Microbiome.

To explore the rice seed microbiome, our objective was to isolate novel strains of Xanthomonas, a plant-associated bacterium with diverse lifestyles. Four isolates, anticipated to be Xanthomonas based on morphological features of yellow colonies, were obtained from healthy rice seeds. Phylo-taxono-genomic analysis revealed that these isolates formed monophyletic lineages belonging to a novel species within the genus Luteibacter. Pairwise ortho Average Nucleotide Identity and digital DNA-DNA hybridization confirmed their distinct species status. We propose Luteibacter sahnii sp. nov. as a novel species, with PPL193T = MTCC 13290T = ICMP 24807T = CFBP 9144T as the type strain and PPL201, PPL552, and PPL554 as other constituent members. The fatty acid profile of the type strain is dominated by branched fatty acids like Iso-C15:0, consistent with other members of the genus. The novel species displays non-pathogenic attributes and exhibits plant probiotic properties, protecting rice plants from the leaf blight pathogen X. oryzae pv. oryzae. Production of Indole-3-Acetic Acid (IAA) and genomic regions encoding anti-microbial peptides emphasize its potential contributions to plant hosts. This study underscores the importance of employing a combination of phenotypic and genotypic methods in culturomics to enhance our understanding of rice seed microbiome diversity.

Fatty Acid Profiling of Major Fatty Acids in Safflower Genotype

Fatty acid profiling is a process of analyzing the composition and quantity of different fatty acids. Fatty acids are organic molecules that are essential for a variety of biological processes in the body, including energy storage and membrane function. Safflower seed contains major four fatty acids viz. linoleic acid, Oleic acid, palmitic acid and stearic acid. The analysis of fatty acid profiles is important for understanding the nutritional quality of the oil and products derived from it. According to hull types of seeds, the seed oil content ranges from 20 % to 45 %. Fatty acid profiling of safflower involves analyzing the types and quantities of fatty acids present in safflower oil. This can provide insights into its nutritional value, stability, and potential health benefits from the oil derived from each genotype. This research is conducted to know the content of saturated as well as unsaturated fatty acid in safflower oil from selected genotypes. The major four fatty acid composition of safflower genotypes reveals palmitic acid content ranges from 5.09% (GMU-2969) to 6.93% (GMU-3929). The stearic acid content ranged between 1.69% (GMU-2969) to 3.55% (GMU-3780). The oleic acid content ranged from 12.88% (GMU-2749) to 20.69% (GMI-3177) and the percent linoleic acid content ranged from 71.31 (GMU-3177) to 78.44% (GMU-2749).

Exploring the synergy of enzymes, nutrients, and gene networks in rice starch granule biogenesis.

Rice is a primary food source almost for more than 50% of the total world's population. Glycemic index (GI) is high in most of the rice varieties, limiting their consumption by diabetic and obese people. As a result, developing new rice varieties with low GI necessitates a thorough understanding of starch biogenesis gene expression and its interrelationship. A total 200 rice genotypes were analyzed for total starch content (TSC), amylopectin content (APC), and amylose content (AC). The clustering of these rice genotypes was done based on their AC. Further, these genotypes were categorized into three groups up to 10% amylose-low, 10-26% amylose-medium, and more than 26% amylose-high. Among them, six genotypes 1 from low AC (NJ-72), 2 from medium AC (UPRI-2003-18, PRR-126), and 3 from high AC (RNRM-7, Urvashi and Ananga) were selected. The genotypes selected from the medium and high AC groups were having 2% amylose variation among themselves respectively and they were further used to study the level of RS, protein content (PC), fatty acid (FA) profiles, and granule morphology along with low group sample. Resistant starch (RS) content ranged from 0.33-2.75%, and fatty acid profiling revealed high levels of palmitic, linoleic, and oleic acids. The degree of crystallinity and APC% were found to be positively correlated. Ananga, the genotype with the highest RS, displayed compact starch granules. Further, NJ-72 showing low RS and Ananga with high RS were selected for investigation of enzymatic activities of starch biosynthesis, metabolites accumulation, and expressions of 20 starch biogenesis genes in developing endosperm. Starch branching enzymes (SBE) and starch synthase (SS) activities peaked at 13 days after anthesis (DAA), while starch debranching enzymes (DBE) were most active at 18 DAA. In Ananga, TSC, AC, APC, and RS levels progressively increased from 3 to 23 DAA. Ananga showed 1.25-fold upregulation of granule-bound starch synthase I (GBSSI) at 18DAA. Higher expressions of SSI and SBEIIb were observed in NJ-72 at 13DAA. PUL2 was predominantly expressed followed by ISA1. GBSSI was positively correlated with both AC and RS while SS, SBE, and DBE were positively related to APC. This research could lead to the development of rice varieties with improved nutritional qualities, such as higher RS content, which is beneficial for human health due to its role in lowering glycemic response and promoting gut health. Additionally, the study provides insights into how the modulation of key genes and enzymes can affect starch composition, offering strategies to breed rice varieties tailored for specific dietary needs or industrial applications.

Effect of supplementation to offspring during early gestation and the growing phase with different sources of fatty acids on learning and memory ability of post-weaning lambs

Abstract The objective of the current experiment was to evaluate the effects of supplementation with different dietary fatty acid profiles on the dam during the first third of gestation and on the offspring during growth on the offspring’s cognitive behavior. Seventy-nine post-weaning lambs were blocked by body weight and sex using a 2×2 factorial arrangement of treatments. The first factor (maternal supplementation; MS) was supplementation to the ewes in the first third of gestation with 1.61% Ca salts of palm fatty acid distillate (PFAD) or Ca salts enriched with eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) to early pregnant ewes. The second factor (offspring supplementation; OS) was to supplement the offspring during their growing phase with diets that contained 1.48% of PFAD or Ca salts of PFAD or EPA and DHA. Ewes were housed in groups, three ewes per pen and 12 pens per treatment, with different treatments until day 50 of gestation. From day 51 of gestation until weaning, all the animals (ewes and lambs) were housed in a common pen. After weaning, lambs were housed in group pens (5 pens per treatment, three to five per pen). The lambs ran maze tests on weeks five and seven after weaning to evaluate cognitive ability. The maze contained two trap zones and had the pen conspecific lambs at the end of the maze. The measurements were the times to solve the traps and the total time to complete the maze. Data were analyzed using a mixed procedure considering the 2×2 factorial arrangement of treatments. There was a MS × OS × time interaction for the time to complete the maze (P = 0.02). Lambs receiving a different type of fatty acid supplementation during gestation than post-weaning took less time to complete the maze on the second relative to the first day compared with the lambs fed the same type of fatty acids during gestation and growing. In conclusion, combining different fatty acids during different life stages may improve lambs' cognitive abilities.

Presence of Soya in Industrial and Homemade Sausage Production in Kosovo and Its Reflection on Fatty Acid Profile

In the present study, we investigated to what extent soybean was used in industrial and homemade sausage produced in Kosovo, as well as its potential impact on the fatty acid profile. In total, 63 samples, 42 industrial and 21 traditional sausages, were collected either from the market or the production site. All samples were tested by means of real-time PCR for the detection and quantification of soy content, as well as the GC-FID to determine the potential reflection in the fatty acid profile. The presence of soy DNA was detected in 54 out of 63 samples. In total, 41 out of 42 industrial sausage samples were positive for soy DNA, with an average ct value of 22.60 and a standard deviation (SD) of 4.28, whereas 13 out of 21 traditional sausage samples were positive for soy DNA, with a mean ct value of 23.36 and a standard deviation (SD) of 4.56. There was a statistically significant difference in means between industrial sausage and traditional sausage, with p ≤ 0.001 based on CT values (ANOVA test). We investigated the correlation between ct values in real-time PCR for soy DNA with each fatty acid content. There is a moderate correlation of soy DNA ct values with C16:0 palmitin (decrease), C18:0 stearic acid (decrease), C18:1 oleic acid (increase), and overall saturated fatty acids (decrease). With the exception of C14:0 Myristin, C18:1 oleic, and C20:0 Arachin acids and monosaturated fats, the ANOVA test reveals a significant difference in means between groups for the majority of the fatty acids between industrial sausages and traditional sausages. The current study demonstrates that the fatty acid composition of sausages is influenced by the amount of soy present in them. The extent to which other components affect the fatty acid profile is unknown. However, an increase in oleic acid and a decrease in stearic and overall saturated fatty acids are expected.

Ultrasound-assisted extraction vs. microwave-assisted extraction for sustainable development goals: Selecting the ideal lipid extraction and fatty acid profile

The objective of this study was to compare the efficiency of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) methods for extracting lipids from the green microalga Chlorococcum novae-angliae. This study specifically focused on the fatty acid profiles of the extracted lipids, using ethanol as the solvent and wet biomass as the starting material. Ultrasound-assisted extraction yielded a maximum of 0.026 ± 0.001 g lipid/g wet biomass at a biomass ratio of 1:25 for 2 min with a 1-second cycle at 180 W and 20 kHz, which was 21% higher than that of microwave-assisted extraction conducted at a ratio of 1:30 for 2 min at 300 W and 35 °C. Ultrasound-assisted extraction enhanced saturated fatty acids (SFAs), which were 1.5 times higher than to microwave-assisted extraction, while microwave-assisted extraction significantly increased polyunsaturated fatty acids (PUFAs) by 4.4 times. The findings suggest that ultrasound-assisted extraction is more suitable for applications requiring high SFA content, such as in the fuel industry, whereas microwave-assisted extraction is preferable for sectors focused on fatty acid quality, such as food and health. This comparative analysis contributes to the literature by highlighting the impact of extraction methods on fatty acid profiles and supports sustainable development goals (SDGs), particularly SDG 12, by promoting environmentally friendly extraction techniques.

Lactobacillus reuteri or Lactobacillus rhamnosus GG intervention facilitates gut barrier function, decreases corticosterone and ameliorates social behavior in LPS-exposed offspring

Probiotic therapy with Lactobacillus reuteri and Lactobacillus rhamnosus (LGG) demonstrates potential as an adjunctive treatment for autism spectrum disorder (ASD). In a rat model of ASD induced by lipopolysaccharide (LPS) injection during pregnancy, we evaluated the effects of these probiotics on offspring. Administration of L. reuteri or LGG for three weeks post-birth improved social deficits and reduced anxiety in LPS-exposed rats. Additionally, probiotics significantly modified short-chain fatty acid profiles, increasing butyric acid levels and decreasing propionic acid levels. They also enhanced colonic barrier integrity by upregulating tight junction proteins, including ZO-1, Occludin, and Claudin4. RNA sequencing identified differential gene expression in pathways related to inflammation, the HPA axis, and reactive oxygen species metabolism, with NADPH oxidase 1 (NOX1) emerging as a crucial gene. Validation studies confirmed that Lactobacillus strains reduced inflammatory cytokines, inhibited corticosterone secretion, increased antioxidant levels, and suppressed the NF-κB/NOX1 pathway. In an H2O2-induced oxidative stress model using Caco-2 cells, pre-treatment with L. reuteri, LGG, or NF-κB inhibitors enhanced cellular antioxidants, inhibited NF-κB/NOX1 activation, and improved barrier function. Overall, L. reuteri and LGG administration improved social behavior, bolstered colonic barrier function, and mitigated HPA axis overactivation in LPS-exposed rats, while also alleviating oxidative stress in the colon and Caco-2 cells. These findings suggest that L. reuteri and LGG have substantial clinical potential for ASD treatment by targeting multiple pathophysiological mechanisms, including inflammation, HPA axis dysregulation, and oxidative stress, thereby presenting a promising adjunctive therapeutic strategy for enhancing social behavior and gut health in ASD.

Impact of fat profile on the rheological and structural characteristics of cup cake

Fats are one of the main ingredients of cakes. The aim of this study is to evaluate the impact of palm-based fat (PBF), lauric-based fat (LBF), and sunflower oil (SFO) on the physicochemical, structural, and rheological properties of cakes. Also, the fatty acid profile (by GC) and the solid fat content (by NMR) were evaluated. The dispersion of fats and oil in the cake's structure was investigated by confocal laser scanning colorimetry (CLSM). The SFC profile showed initial crystallization during the cooling time at a constant temperature, starting with LBF > PBF > SFO respectively. The viscosity of treatments showed a shear-dependent behavior, and the elastic (G′) and viscous (G″) modulus of the batter increased by increasing the frequency. Moreover, the hardness, cohesiveness, springiness and chewiness of samples with different fats and oil has a type and time trend. The CLSM image revealed that the PBF-treated cake had more uniform structure in addition to well-distributed fat spots in the constant area. The studies showed that liquid oil cannot contribute to covering other ingredients to form a stable structure, while samples containing LBF and PBF are capable of surrounding all ingredients, which results in a higher-quality product.

Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves.

Larval cryopreservation techniques have been established in Pacific oysters and Mediterranean mussels. Although initial cryopreservation protocols for both species differed slightly in Ficoll PM 70 (FIC) concentration, better post-thaw larval survivability was produced in mussels than oysters. Furthermore, the post-thaw survivability in the latter could be improved significantly by the addition of lipids and antioxidants to the cryoprotectant agent. These findings have generated a unique opportunity to investigate the cryo-functions of both endogenous and exogenous fatty acids (FAs) in bivalves as mammalian studies indicated that lipid compositions could contribute the interspecific difference in gamete and embryo cryopreservation. Our study showed that the higher survivability of post-thaw larvae in mussels (65% vs. 34% in oysters) could be attributed to their higher proportion of total polyunsaturated fatty acids (PUFAs), especially C22:6, and higher resistance of FA profiles to cryopreservation. In oysters, on the other hand, their FA profiles were very sensitive to cryopreservation, with saturated FAs and PUFAs being significantly increased (from 37 to 41%) and decreased (from 35% to ~ 32%), respectively. Although exogenous lipids could significantly improve the survivability of post-thaw oyster larvae from 34 to 51%, their supplementation did not alter the response pattern of endogenous FA profiles to cryopreservation.

Characterization and Evaluation of Taihe Black-Boned Silky Fowl Eggs Based on Physical Properties, Nutritive Values, and Flavor Profiles.

Taihe black-boned silky fowl (TS) is a native chicken breed in China with more than 2000 years of history. The present study aimed to characterize and evaluate the physical, nutritional, and flavor properties of TS eggs with a comparison to two other commercial breeds. Eggs from TS (n = 60) crossbred black-boned silky fowl (CB, n = 60) and Hy-line Brown (HL, n = 60) were used for physicochemical analysis. The evaluation system was divided into four parts based on nutrient and flavor profiles: protein and amino acids, lipids and fatty acids, mineral elements, and flavor-related amino acids and volatile compounds. Results showed that TS eggs were typically associated with the lowest egg weight and the highest yolk color, as compared with CB and HL eggs. No differences were found in crude protein, crude fat, triglycerides, and cholesterol content between eggs from the different breeds, but these eggs were distinct in terms of the amino acid, fatty acid, and volatile flavor compound profiles. Moreover, the differences in amino acid and fatty acid profiles might contribute to the specific flavor of TS eggs. Evaluation results indicated that TS egg whites may be suitable as a protein source for premature infants and young children under three years old and TS egg yolks could be considered a beneficial dietary lipid source due to their potential anti-cardiovascular properties. Additionally, TS whole eggs could serve as a valuable source of selenium (Se), molybdenum (Mo), zinc (Zn), and phosphorus (P) for adults aged 18 to 65. However, TS and CB eggs showed inferior Haugh units, eggshell quality, and essential amino acid compositions for older children, adolescents, and adults. These findings provide a better insight into the health benefits of TS eggs and contribute to the breeding and nutrition regulation of TS breeds.