Fatty Acid Profile Of Beef Research Articles

Possibility of using fatty acid profiles for the authentication of beef adulterated with pork, donkey, and dog meat

Detection of meat adulteration is a critical issue in food labeling procedures and a serious concern related to food fraud, authenticity, and religious beliefs. The current work detected and quantified adulteration of raw ground beef with pork, donkey, and dog meat based on fatty acid profiles using GC-MS/MS. The study design incorporated pork, donkey, or dog meat with beef meat; negative and positive controls were used for the different meat species. Results demonstrated several significant differences (p < 0.05) in fatty acid contents between mixed/adulterated meat and pure beef. In addition, higher total saturated fatty acid levels in ground beef (57.91%) compared to dog fat (46.44%), donkey (38.71%), and pork fat (lard) (40.23%). High total unsaturated fatty acids content was observed in donkey (61.92%), pork (59.77%), and dog (53.56%) fats compared to beef fat (42.09%). On the other hand, total unsaturated and monounsaturated fatty acids in beef meat were lower than in pork, donkey, and dog meat. Moreover, the highest trans-fatty acid content was found in pork compared to the other meat types. All incorporated samples correlated positively with pure ground beef concerning the fatty acid profiles. Therefore, alteration of the mixed beef fatty acid profiles was a potential indicator for adulteration since a marked decrease in total saturated fatty acid content and an increase in unsaturated fatty acids was observed in the substituted samples. We concluded that GC-MS/MS-based fatty acid profiling is a promising technique that can be used to detect meat adulteration.

PSX-14 Effects of Forage and Grain-Based Finishing Diets on Fatty Acid Profile of Angus Steers Backgrounded Either in a Moderate Or in a High Plane of Nutrition

Abstract The objective of this study was to evaluate the fatty acid profile of beef backgrounded in two planes of nutrition and finished with forage-only or corn. Steers (n = 24) weighing approximately 298.63 ± 10.19 kg were randomly backgrounded in Triticale (100% DM; Moderate n = 12) and alfalfa (85% DM) and beardless wheat (15% DM; High, n = 12), blocked, and finished with forage-only (FORAGE – Alfalfa with 21% CP, 100% DM, n = 12) or with a grain-based diet (GRAIN - Alfalfa with 16% CP, 80% DM and Corn, 20% DM, n = 12). The feeding trial lasted 220 days (85 d backgrounding, 30 d finishing diet adaptation, and 105 d finishing). The fatty acid profile of strip loin steaks (m. longissimus lumborum) was assessed, and data were analyzed as a 2x2 factorial. Overall, no interaction effect between plane of nutrition and finishing diet was observed for individual or group of fatty acids. Minimal effects were observed for plane of nutrition. Steaks from steers backgrounded in Moderate had greater concentrations of C14:1T n-5 (P = 0.04) and C18:1TΔ n-9 (P = 0.04) when compared with steaks from steers backgrounded in High. Beef from steers finished with FORAGE showed higher concentrations of C10:0 (P = 0.01), C14:1T n-5 (P < 0.0001), C15:0 (P = 0.003), C16:0 (P = 0 .001), C16:1T n-7 (P < 0.0001), C17:0 (P = 0.001), C18:0 (P = 0.0002), C18:1TΔ n-9 (P = 0.0003), C18:2T n-6 (P = 0.005), C20:1 n-12 (P < 0.0001), C18:3 n-3 (P = 0.0003), 18:2 CLA t10c12 (P < 0.0001), C22:1 n-9 (P = 0.02), C22:2 n-6 (P = 0.001), and C22:5 n-3 (P = 0.02) when compared with beef from steers fed GRAIN. Beef fed GRAIN, had greater concentrations of C16:1 n-7 (P = 0.001), C18:1 n-9 (P = 0.004), C18:1Δ n-11 (P < 0.0001) and C18:2 n-6 (P = 0.02) when compared with beef fed FORAGE. Overall, backgrounding steers in moderate and high planes of nutrition does not affect the fatty acid profile of beef. Beef fed with FORAGE has higher total Omega 3 (P = 0.05), total Saturated (P < 0.0001), and total Trans fatty acids (P < 0.0001) when compared with beef fed GRAIN. GRAIN-fed beef has total Monounsaturated fatty acids (P = 0.0002) and higher Omega6:Omega3 (P = 0.0003). Regarding health indexes, based on its fatty acid profile, FORAGE-fed beef had Atherogenicity (P = 0.01) and Thrombogenicity indexes (P = 0.0001) when compared with beef fed GRAIN (Table 1).

Effects of dental age on proximate components, amino acid and fatty acid profiles of indigenous beef

The study was conducted to determine how the proximate composition, amino acid and fatty acid profiles of beef of indigenous cattle are affected by dental age. This experiment was conducted with five (5) treatments (T1, T2, T3, T4, and T5) where T1 = 0 Permanent incisors, T2 = 2 Permanent incisors, T3 = 4 Permanent incisors, T4 = 6 Permanent incisors, T5 = 8 Permanent incisors having ten (10) replications. This study has shown that the highest CP was 84.91% having the T5 while the lowest figure was 72.94% in T4. T1 had the lowest 3.34% EE content whereas T5 had the highest 4.15%. Essential amino acids (g/100g of meat) were highest at 41.51 in T5 and lowest at 37.79 in T1. The lowest total saturated fatty acid (SFA) in Longissimus dorsi was 46.25% in T5 and the highest was 51.89% in T1. The total monounsaturated fatty acid (MUFA) proportion of 66.87% in T2 was the highest and T5 had the lowest proportion of 52.07%. The lowest total polyunsaturated fatty acid (PUFA) was 1.52% in T1 and the highest was at 7.49% in T5. In conclusion, CP, EE, essential amino acid, and polyunsaturated (PUFA) fatty acid proportion increased with the advancement of dental age maturity and conversely, saturated fatty acid (SFA) proportion was decreased. Therefore, this research will play a vital role to characterize accurately in terms of its nutritional quality to be aware of the selection of indigenous beef in their daily diet according to dental age.

63 Fatty Acid Profile of Beef from Feedlot Young Bulls of Two Genetic Groups

Abstract The fatty acid profile of the longissimus thoracis (LT) muscle and the activity of Δ9 desaturase6, Δ9 desaturase8, and elongase enzymes of beef from feedlot cattle of 2 genetic groups were evaluated. Fifty long-yearling young bulls were divided into two groups following their breed composition: Nellore (n = 25) and Angus × Nellore crossbred (n = 25), were randomly assigned into 4 pens (12 or 13 animals/pen) following a completely randomized design. Animals were sourced from the same breeding season and endured a grazing growing phase in Megathyrsus maximus cv. Mombaça pasture, with protein and energy supplement offered at 0.2% of body weight (BW). The feedlot phase comprehended 105 days of feed, and the diet (80% concentrate) was formulated to meet or safely exceed the beef cattle requirements to target an average daily gain (ADG) of 1.6 kg/day, in which major ingredients were represented by (% DM basis): sorghum silage (20%), ground corn (57.6%), ground soybean seed as the lipid source (20.4%), and a mineral/vitamin premix (2%). Samples of the LT muscle (between the 12th and 13th ribs) were taken right after slaughter to determine the profile of intramuscular fat. Lipids were extracted (2:1 chloroform/methanol), and 5 mL of the lipid extract was used for saponification followed by methylation and submitted for analysis using gas chromatography. The activities of Δ9 desaturase6, Δ9 desaturase8, and elongase enzymes were determined through mathematical indexes, in which their respective saturated and monounsaturated fatty acids were used. Data were analyzed using R (R CORE TEAM, 2021), and the effect of breed on the fatty acid profile was evaluated using the completely randomized design one-way, in which animal was used as the experimental unit. The sum of saturated fatty acids and unsaturated fatty acids was not affected (P ≥ 0.12) by genetic groups. Within FA profiles, crossbred bulls had greater (P = 0.05) concentration of monounsaturated fatty acids (49.7 vs. 52.4%), while beef of Nellore cattle showed greater (P ≤ 0.02) concentration of polyunsaturated fatty acids (6.71 vs. 7.67%) and n-3 (0.53 vs. 0.68%). A tendency (P = 0.07) was observed for the sum of n-6 and n-6/n-3 ratio, in which Nellore cattle had numerically greater values (6.63 vs. 4.89%, and 9.75 vs. 9.22, respectively). The activity of Δ9 desaturase6 and Δ9 desaturase8 was not affected (P ≥ 0.14) by genetic groups, while crossbred animals had greater (P = 0.03) activity of elongase (67.8 vs. 65.6%). Although the overall sum of mono and polyunsaturated fatty acids seems not to be affected, the FA profile and unsaturation/elongation enzymatic activity of beef was influenced by the genetic groups evaluated, in which both Nellore and their F-1 crossbreed counterparts had attractive responses.

Polimorfismo dos genes DGAT e LEP e sua associação com características de carcaça e perfil lipídico da carne de bovinos nelore

ABSTRACT The objective of this study was to evaluate the association of polymorphisms in the diacylglycerol acyltransferase ( DGAT ) and leptin ( LEP ) genes with the performance, carcass characteristics, meat quality, and lipid profile of Nellore cattle. A total of 100 intact male Nelore cattle were used to analyze the performance, carcass, physicochemical and centesimal composition, and fatty acid profile of beef. To identify the polymorphisms, the PCR–single-strand conformation polymorphism (SSCP) technique was applied to genomic DNA extracted from muscle tissue. The SSCP technique revealed the presence of four band patterns for the DGAT gene (AC, AD, AE and BB) with five alleles (A, B, C, D and E). For the LEP gene, five band patterns (AA, AB, AC, BB and BC) with three alleles (A, B and C) were observed. For the LEP gene, the AB genotype was associated with higher backfat thickness and ribs weight, while the BB genotype was associated with lower ribs yield; higher hindquarter yield was associated with AC and BB genotypes. Higher contents of C17:0, C18:0 and lower contents of C18:2ω6C, total polyunsaturated fatty acids, total ω6 and ratio of polyunsaturated and saturated fatty acids (PUFA/SFA) were verified for the AC genotype of the LEP gene. The AC and AA genotypes of the LEP gene were associated with higher means of C15:0 and C18:1ω9t. For the DGAT gene, the highest C24:0 content was associated with the AE genotype and the lowest with the AD and BB genotypes. Polymorphisms in the DGAT and LEP genes influence carcass parameters and the lipid profile of the meat of Nellore cattle.

KOMPOSISI ASAM LEMAK PADA DAGING SAPI RANCAH DAN PERANAKAN ONGOLE

Local beef is known for its low-fat content/lean meat. The fatty acid profile of beef could affect the meat quality, related to its texture, taste, and nutritional value. This study aims to determine the fatty acid profile of Rancah and Ongole Grade beef. This study used fourteen samples of meat from Rancah cattle 1.5 – 2.5 years, which were extensively reared with forage feed, and nine samples of Ongole Grade beef with forage and concentrate feeding as much as 2.5% dry matter of body weight. A fatty acid profile was analyzed from the longissimus dorsi muscle area, between the 12th and 13th ribs. The fatty acid profiles identified are saturated and unsaturated fatty acids (MUFA and PUFA). The fatty acid analysis used is the Gas Chromatography (GCMS) method. The data were analyzed descriptively and fatty acid composition differences of Rancah and Ongole grade beef were analyzed by T-test. The results showed that the fatty acid composition of Rancah and Ongole grade beef is relatively the same as the beef of other breeds. Saturated fatty acid in Ongole Grade beef is higher than Rancah beef, but the content of unsaturated fatty acids, PUFA/MUFA: SFA ratio in Rancah beef is higher than Ongole Grade.

Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions

BackgroundHealthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues. Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions). Possibly due to a lack of data, this strategy has never been explored.ResultsThis study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices. We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC). Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA. Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy. These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas). A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity). When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of −0.41±0.12 sd.ConclusionThis research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.6mp-1d5cpTQY_WdEUFHrBcVideo

Integration analyses of structural variations and differential gene expression associated with beef fatty acid profile in Nellore cattle.

This study aimed to integrate analyses of structural variations and differentially expressed genes (DEGs) associated with the beef fatty acid (FA) profile in Nellore cattle. Copy numbers variation (CNV) detection was performed using the penncnv algorithm and CNVRuler software in 3794 genotyped animals through the High-Density Bovine BeadChip. In order to perform the genomic wide association study (GWAS), a total of 963 genotyped animals were selected to obtain the intramuscular lipid concentration and quantify the beef FA profile. A total of 48 animals belonging to the same farm and management lot were extracted from the 963 genotyped and phenotyped animals to carry out the transcriptomic and differentially expressed gene analyses. The GWAS with extreme groups of FA profiles was performed using a logistic model. A total of 43, 42, 66 and 35 significant CNV regions (p < 0.05) for saturated, monounsaturated, polyunsaturated and omega 3 and 6 fatty acids were identified respectively. The paired-end sequencing of 48 samples was performed using the Illumina HiSeq2500 platform. Real-time quantitative PCR was used to validate the DEGs identified by RNA-seq analysis. The results showed several DEGs associated with the FA profile of Longissimus thoracis, such as BSCL2 and SAMD8. Enriched terms as the cellular response to corticosteroid (GO:0071384) and glucocorticoid stimulus (GO:0071385) could be highlighted. The identification of structural variations harboring candidate genes for beef FA must contribute to the elucidation of the genetic basis that determines the beef FA composition of intramuscular fat in Nellore cattle. Our results will contribute to the identification of potential biomarkers for complex phenotypes, such as the FA profile, to improve the reliability of the genomic predictions including pre-selected variants using differentiated weighting in the genomic models.

Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle.

The beef fatty acid (FA) profile has the potential to impact human health, and displays polygenic and complex features. This study aimed to identify the transcriptomic FA profile in the longissimus thoracis muscle in Nellore beef cattle finished in feedlot. Forty-four young bulls were sampled to assess the beef FA profile by considering 14 phenotypes and including differentially expressed genes (DEG), co-expressed (COE), and differentially co-expressed genes (DCO) analyses. All samples (n=44) were used for COE analysis, whereas 30 samples with extreme phenotypes for the beef FA profile were used for DEG and DCO. A total of 912 DEG were identified, and the polyunsaturated (n=563) and unsaturated ω-3 (n=346) FA sums groups were the most frequently observed. The COE analyses identified three modules, of which the blue module (n=1776) was correlated with eight of 14 FA phenotypes. Also, 759 DCO genes were listed, and the oleic acid (n=358) and monounsaturated fatty acids sum (n=120) were the most frequent. Furthermore, 243 and 13, 319 and seven, and 173 and 12 gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways were enriched respectively for the DEG, COE, and DCO analyses. Combining the results, we highlight the unexplored GIPC2, ASB5, and PPP5C genes in cattle. Besides LIPE and INSIG2 genes in COE modules, the ACSL3, ECI1, DECR2, FITM1, and SDHB genes were signaled in at least two analyses. These findings contribute to understand the genetic mechanisms underlying the beef FA profile in Nellore beef cattle finished in feedlot.

Soil health and nutrient density: preliminary comparison of regenerative and conventional farming.

Several independent comparisons indicate regenerative farming practices enhance the nutritional profiles of crops and livestock. Measurements from paired farms across the United States indicate differences in soil health and crop nutrient density between fields worked with conventional (synthetically-fertilized and herbicide-treated) or regenerative practices for 5 to 10 years. Specifically, regenerative farms that combined no-till, cover crops, and diverse rotations—a system known as Conservation Agriculture—produced crops with higher soil organic matter levels, soil health scores, and levels of certain vitamins, minerals, and phytochemicals. In addition, crops from two regenerative no-till vegetable farms, one in California and the other in Connecticut, had higher levels of phytochemicals than values reported previously from New York supermarkets. Moreover, a comparison of wheat from adjacent regenerative and conventional no-till fields in northern Oregon found a higher density of mineral micronutrients in the regenerative crop. Finally, a comparison of the unsaturated fatty acid profile of beef and pork raised on one of the regenerative farms to a regional health-promoting brand and conventional meat from local supermarkets, found higher levels of omega-3 fats and a more health-beneficial ratio of omega-6 to omega-3 fats. Despite small sample sizes, all three crop comparisons show differences in micronutrient and phytochemical concentrations that suggest soil health is an under appreciated influence on nutrient density, particularly for phytochemicals not conventionally considered nutrients but nonetheless relevant to chronic disease prevention. Likewise, regenerative grazing practices produced meat with a better fatty acid profile than conventional and regional health-promoting brands. Together these comparisons offer preliminary support for the conclusion that regenerative soil-building farming practices can enhance the nutritional profile of conventionally grown plant and animal foods.

Carcass traits, fatty acid profile of beef, and beef quality of Nellore and Angus x Nellore crossbred young bulls finished in a feedlot

The objective of this study was to evaluate carcass traits, fatty acid profile of longissimus thoracis (LT) muscle, and beef quality (shear force and color traits) of Nellore (NEL) and Angus x Nellore (AN) crossbred young bulls finished in feedlot. For this end, 25 Nellore (Initial body weight IBW = 344.4 ± 30 kg) and 25 Angus x Nellore crossbreed bulls (IBW = 426.5 ± 27 kg) were evaluated. The AN animals had higher final body weight (FBW), average daily gain of carcass (ADGc), hot carcass weight (HCW), rib-eye area (REA), and final pH in LT muscle, while NEL had higher subcutaneous fat thickness (SFT) (P < 0.01). The LT muscle of NEL had lower concentrations of saturated fatty acids (SFA) and higher concentrations of monounsaturated fatty acids (MUFA) and poliunsaturated fatty acids (PUFA) besides a higher Δ9-desaturase index (P < 0.05). There was a difference in color traits between breeds and among aging time points (P < 0.05). Shear force was influenced only by aging time (P < 0.05). The crossbred animals showed improved quantitative carcass traits compared to purebred Nellore animals. Breed has the potential to change muscle FA profile. The crossbred animals were not able to produce a more tender beef when compared to NEL.

Fatty acid profile and beef quality of Nellore and Angus bulls fed whole shelled corn

This study aimed to evaluate the quality traits and fatty acid profile of beef from Nellore and Angus bulls fed whole shelled corn (WSC) and ground corn plus maize silage (GC) diets. Eighteen Nellore and 18 Angus young bulls [381 ± 12 kg initial body weight (BW) and an average age of 20 ± 1.9 months] were used in a completely randomized design using a 2 × 2 factorial arrangement and were slaughtered at a final BW of 451.5 kg and 545.5 kg, respectively. Twenty–four hours after slaughter, samples of longissimus thoracis muscle were collected for the analysis of lipid oxidation, color, fatty acid profile, shear force, and cooking loss. There was no effect of diet × breed interaction on meat color, lipid oxidation, shear force, and cooking loss. Angus beef had lower shear force (p < 0.05) than Nellore beef and had a greater concentration of linoleic acid and polyunsaturated fatty acids (PUFA) (p < 0.01). Beef of bulls fed WSC tended to have greater concentration of CLA C18:2c9t11 (p = 0.09), greater concentration of CLA C18:2t10c12 (p = 0.01), and PUFA (p = 0.05), and consequently, higher oxidation levels. Angus bulls produced beef with greater tenderness and PUFA concentration. The results of fatty acid show a possible change in biohydrogenation when animals are fed the WSC diet, reducing lipogenesis, as this diet increases the C18:2t10c12 content.

Subprodutos agroindustriais de caroço de algodão e soja utilizados na dieta de bovinos confinados: efeitos no perfil de ácidos graxos e parâmetros de qualidade da carne bovina

ABSTRACT Beef composition are directly influenced by the dietary fat, particularly in ruminants, due to rumen biohydrogenation, which promotes alterations in the dietary fatty acid (FA) profile and affects the meat quality. Beef sensorial, chemical, and FA compositions of intramuscular and subcutaneous fat were evaluated, from 40 Nellore males finished on feedlot diet based, containing cottonseed (CSB) and soybean by-product (SOB) as agro-industrial by-product fat sources. CSB as a fat source, did not alter the beef pH, shear force, chemical composition, or subcutaneous FA profile compared with the SOB diet. Differences were observed at yellow and red beef color, with low and high CSB diet inclusion; on the contrary, inclusion of SOB in the diet led to an intensely unpleasant aroma in aged and cooked meats. Regarding beef FA profile, CSB with 3% dietary fat produced steaks with a lower proportion of C 17:0 , having 1.359 and 3.238 g/100 g of intramuscular FA, whereas CSB with 5% dietary fat led to an increase in C 18:2 n -6 , with 0.298 and 0.132 g/100 g of intramuscular FA in steaks from animals fed with CSB and SOB, respectively. CSB produced more intense red beef color, unpleasant aroma, and higher linoleic acid content; however, the color and aroma of the meat produced from cattle fed with CSB in the diet were less intense and were like those observed in cattle fed with SOB; these could therefore be recommended for use as agro-industrial by-products in beef cattle diet.

Fattening of Polish Holstein-Friesian × Limousin Bulls under Two Production Systems and Its Effect on the Fatty Acid Profiles of Different Fat Depots

Simple SummaryCattle raised under different production systems differ in their ability to accumulate adipose tissue. Whereas the deposition of intramuscular fat is usually considered beneficial, excessive amounts of subcutaneous and internal fat are undesirable. This study compared the fatty acid (FA) profiles of four types of fat depots from crossbred bulls (Polish Holstein-Friesian × Limousin, PHF × LM) fattened semi-intensively (SI) and intensively (I). Intramuscular fat was most abundant in total polyunsaturated fatty acids (PUFAs), n-3 and n-6 PUFAs, and functional fatty acids C 18:2, C 18:3, C 20:4 and C 20:5 in comparison with the remaining fat types. Furthermore, note that external fat was more abundant in conjugated linoleic acid (CLA) than other fat types. This finding may have implications for both the beef industry and, due to the observed differences in FA composition, human health.This study was designed to compare the fatty acid (FA) profiles of four types of fat depots from crossbred bulls (Polish Holstein-Friesian × Limousin, PHF × LM) fattened semi-intensively (SI) and intensively (I). Intramuscular fat was most abundant in total polyunsaturated fatty acids (PUFAs), n-3 and n-6 PUFAs, and functional fatty acids C 18:2, C 18:3, C 20:4 and C 20:5 in comparison with the remaining fat types. Furthermore, note that external fat was more abundant in conjugated linoleic acid (CLA) than other fat types. Grass silage fed to cattle during fattening had a beneficial influence on the FA profile of beef, and an increase in the amount of concentrate in the ration did not decrease beef quality.

Genome-wide interaction study reveals epistatic interactions for beef lipid-related traits in Nellore cattle.

Gene-gene interactions cause hidden genetic variation in natural populations and could be responsible for the lack of replication that is typically observed in complex traits studies. This study aimed to identify gene-gene interactions using the empirical Hilbert-Schmidt Independence Criterion method to test for epistasis in beef fatty acid profile traits of Nellore cattle. The dataset contained records from 963 bulls, genotyped using a 777 962k SNP chip. Meat samples of Longissimus muscle, were taken to measure fatty acid composition, which was quantified by gas chromatography. We chose to work with the sums of saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA), omega-3 (OM3), omega-6 (OM6), SFA:PUFA and OM3:OM6 fatty acid ratios. The SNPs in the interactions where were mapped individually and used to search for candidate genes. Totals of 602, 3, 13, 23, 13, 215 and 169 candidate genes for SFAs, MUFAs, PUFAs, OM3s, OM6s and SFA:PUFA and OM3:OM6 ratios were identified respectively. The candidate genes found were associated with cholesterol, lipid regulation, low-density lipoprotein receptors, feed efficiency and inflammatory response. Enrichment analysis revealed 57 significant GO and 18 KEGG terms ( < 0.05), most of them related to meat quality and complementary terms. Our results showed substantial genetic interactions associated with lipid profile, meat quality, carcass and feed efficiency traits for the first time in Nellore cattle. The knowledge of these SNP-SNP interactions could improve understanding of the genetic and physiological mechanisms that contribute to lipid-related traits and improve human health by the selection of healthier meat products.

Genome-wide association study for beef fatty acid profile using haplotypes in Nellore cattle

The objective of this study was to identify genomic regions associated with beef fatty acid (FA) profile in Nellore cattle finished in feedlot, using haplotype based analyzes. Also, the metabolic pathway enrichment analysis was performed to seek for genes with known functions, close to the haplotype blocks, associated with the evaluated traits, providing subsidies for a better understanding of how the genes affect this trait. A total of 963 Nellore bulls with phenotype for FA, were genotyped using the Illumina BovineHD BeadChip. Samples from the Longissimus thoracis muscle were taken for FAs profile measurement by gas chromatography using a SP-2560 capillary column. Haplotype blocks were defined based on the linkage disequilibrium (LD), where pairs of SNPs are in strong LD if the confidence interval is equal to or greater than 95% with D’ equal to 0.98 and the lower limit is above 0.70. The model used to estimate the haplotypes effects for the sum of beef fatty acid groups included the fixed effects of contemporary group, animal age at slaughter (as linear covariable), haplotypes (as linear regression over the number of haplotype copies: 0, 1 e 2), and the additive genetic effect as random (polygenic effect). Genome-wide association analyzes were performed for each haplotype block (single marker association) by a Restricted Maximum Likelihood model (REML) using ASREML v. 4.3 software. A total of 15, 15, 4, 3, 6, 5, and 10 haplotypes were significantly associated (FDR< 0.10) with monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), total amount of saturated fatty acids (SFA), PUFA:SFA ratio, omega 3 (n3), omega 6 (n6), and n6:n3 ratio, respectively. The statistically significant haplotypes harbored 38 genes, among which we highlighted those involved in lipid biosynthesis and metabolism, low-density lipoprotein oxidation, transport of fatty acids and cholesterol, adipocyte functions, biosynthesis of glycoprotein hormones, regulation of serum triglyceride and high-density lipoprotein-cholesterol levels, and adipogenesis. In addition, several novel regions were identified, including a putative quantitative trait locus for lipid metabolism and energy homeostasis. The enrichment analysis by DAVID tool revealed 14 significant (P< 0.05) terms related to fatty acids such as cellular response to retinoic acid, glycerol transport, regulation of Notch signaling pathway, and Ras signaling pathway. These results would contribute to the improvement of nutritional and health value of beef lipids in indicine cattle by including information from causal mutations in genetic evaluation through weighted their effects differently in genomic predictions.

Proximate Composition and Fatty Acid Profile of Beef from Arsi, Borana and Harar Cattle Breeds in Oromia National Regional State, Ethiopia

This study was conducted to determine proximate composition and profile of fatty acid of beef from Arsi, Borana and Harar Cattle breeds in Ethiopia. A total of 39 bulls with three age categories were used for the study. The bulls were purchased from mixed crop livestock system (Arsi and Harar cattle breeds) and Ranch (Borana cattle breed). Complete randomized design was used for the study. Longissimus dorsi muscle was used to evaluate proximate composition and profile of fatty acid. The result of the study indicated that mean of percentage of dry matter, ash, crude fat and crude protein were ranging 22.43 - 24.26, 0.32 - 1.28, 4.32 - 7.88, 17.21 - 22.76, respectively. At age younger than 3 years, Harar bulls contain more crude fat compared to Arsi bulls while the vice versa was true for crude protein. The concentration of polyunsaturated fatty acid (PUFA) was higher than saturated fatty acid (SFA) in the three breeds studied across all age categories. However, significantly higher (P 0.01) PUFA and lowest (P 0.05) SFA found in Harar breed whereas the opposite hold true for Arsi bulls. Ratio of n-6: n-3 in the muscle of bulls under the study was ranged from 2.10 to 2.57. Concentration of PUFA and MUF in muscle of the three breeds were significantly affected (P 0.05) by age. From the study it was concluded that Arsi, Boran and Harar bulls under the three age categories contained more than 3% minimum crude fat that is required to insure palatability of the beef. The higher concentration of PUFA over SFA in all breeds across age categories indicated that the meat from these cattle breeds has less risk hazard to human health. However, a strategy needs to be developed to increase the ratio of n-6: n-3 to the nutritional recommendations by the World Health Organization which is 4:1 to 5:1. Moreover, the cause of the difference in proximate composition and profile of fatty acid between breeds under the study at different age categories needs to be investigated.

Canonical discriminant analysis of the fatty acid profile of muscle to authenticate beef from grass-fed and other beef production systems: Model development and validation

The potential of diet-induced differences in the fatty acid profile of muscle to discriminate beef from different feeding systems and its potential use as an authentication tool was investigated. Three canonical discriminant models were built and validated using the fatty acid profile of beef from animals fed solely on pasture or cereal-based concentrates for 11 months or on various pasture/grass silage/concentrate combinations, including concentrates enriched with plant oils. Results indicated that models could successfully discriminate between grass-, partially grass- and concentrate-fed beef (accuracy = 99%) and between grass-fed beef and beef from animals supplemented with plant oils (accuracy = 96%). The approach also showed potential for distinguishing between beef from exclusively pasture-fed cattle and beef from cattle fed on pasture preceded by a period on ensiled grass (accuracy = 89%). Models were also applied to beef samples from 9 different countries. Of 97 international samples, including samples stated to be grass-fed, only 5% were incorrectly classified as Irish-grass-fed beef. These results suggested that the models captured traits in the fatty acid profile that are characteristic of Irish grass-fed beef and that this feature could be used for distinguishing Irish grass-fed beef from beef from other regions.

250 Evaluation of Green Grass as a feed ingredient in beef finishing rations and impact on cattle performance, carcass characteristics, and fatty acid profiles in meat

Abstract A finishing study used 240 crossbred steers (initial BW=340 ±24 kg) to evaluate performance, carcass characteristics, and fatty acid profiles of beef from finishing steers. Four inclusions (0, 10, 20, 30 % DM basis) of Green Grass displaced dry rolled corn in the finishing diet. Green Grass is a blend of herbs and plants imported from Korea that are high in omega-3 fatty acids with 13.9 % fat,32.0 % CP, and 27.0 % Acid detergent fiber. The remaining diet consisted of 15 % WDGS, 20 % corn silage, and 6 % liquid supplement (DM basis). There were no differences in final BW, gain or carcass traits (P³0.14). Cattle were harvested over three slaughter days and were fed for 190, 199, or 203 days. Dry matter intake linearly increased (P = 0.04)as Green Grass inclusion increased in the diet. A linear response (P£0.01) to feeding Green Grass was observed for G:F, with steers fed Green Grass having lower feed efficiency than control cattle. The G:F of cattle fed Green Grass was 5.4, 3.4, and 6.8 % lower in G:F as inclusion increased suggesting Green Grass has 68.6 % the energy of corn it replaced. Steers fed 30 % Green Grass had a lower marbling score. Linear increases in alpha linolenic acid, trans-unsaturated fatty acids, and omega-3 fatty acids (P£0.01) were observed in steak samples from steers fed increasing inclusion of Green Grass. Including up to 30 % inclusion of Green Grass on a DM basis in finishing diets decreased G:F; however, it appeared to have no other effects on performance or carcass characteristics. Feeding Green Grass linearly improved omega-3 fatty acid concentration in beef.

PSVII-7 Fatty acid profile is not affected by feeding finishing cattle essential oils and/or benzoic acid

Abstract Fatty acid profile of beef subcutaneous fat was evaluated in two separate studies where blends of essential oils and/or benzoic acid were fed to finishing steers. Study 1 had sixty-eight finishing steers that were fed 1 of 5 finishing diets: no additional supplement, monensin/tylosin, essential oil blend #1 (Victus Liv, DSM Nutritional Products), benzoic acid (VevoVitall, DSM Nutritional Products), or a combination of essential oil blend #1 and benzoic acid. Study 2 had seventy-six finishing steers that were fed 1 of 7 finishing diets: no additional supplement, monensin/tylosin, essential oil blend #1, essential oil blend #2 (Fortissa Fit 45, Provimi Canada), benzoic acid, a combination of essential oil blend #1 and benzoic acid, or a combination of essential oil blend #2 and benzoic acid. All feed additives were supplemented according to manufacturer instructions, which were 33 mg/kg of monensin, 11 mg/kg of tylosin, 1 g/steer/day of essential oil blend #1, 4 g/steer/day of essential oil blend #2, and 0.5% inclusion level (on a DM basis) of benzoic acid. Fatty acid profile was determined on a 4 cm × 1 cm × 1 cm sample of subcutaneous fat from the 12th rib location of each steer. Fatty acid methyl esters were used to identify and quantify individual fatty acids using gas chromatography. Data were analyzed separately for the two studies, using a RCBD with fixed effect of treatment, and random effects of block (allocation weight of the steers) and the interaction of treatment and block. Total saturated fatty acids (SFA), total monounsaturated fatty acids (MUFA), total polyunsaturated fatty acids (PUFA), MUFA:SFA, PUFA:SFA, and n6:n3 were not different (P &amp;gt; 0.09) between treatments for either study. Overall, it was concluded that supplementation of essential oils and/or benzoic acid did not affect fatty acid profile of beef subcutaneous fat.