Fat Trim Research Articles

Genetic Markers Related to Meat Quality Properties in Fattened HF and HF x Charolaise Steers.

This study involved 45 Holstein and 60 Holstein-Charolaise steers, tailored with specific diets according to breed and rearing systems. DNA genotyping was conducted for DGAT1, LEP, SCD1, SREBF1, and TG genes to investigate their impact on carcass conformation traits, beef quality traits, and sensory quality traits. The results showed associations between the genetic variants and the analyzed traits. Specifically, DGAT1 was found to affect drip loss, meat brightness, and color saturation. The TG gene was associated with marbling and meat color. LEP influenced trim fat and pH levels, while SCD1 was linked to metabolic energy live weight gains, and pH levels. SREBF1 was related to fatness.

PSV-23 Characterization of Katahdin Lamb Carcasses in a Traditional Lamb Marketing Context

Abstract The Katahdin breed has grown in popularity in the US, but it is relatively unknown if their carcasses conform to expectations of the traditional commodity lamb industry. Purebred Katahdin wethers (n = 223) were sourced from the genetic reference flock at the U.S. Meat Animal Research Center over two consecutive years. The flock is enrolled in the National Sheep Improvement Program (NSIP) and estimated breeding values (EBV) were available on standard production traits. Lambs were assigned to one of three groups per year based on body weight (BW) and fed a common finishing diet through harvest at similar end BW (51.3 ± 5.6 kg) but varying age (223.8 ± 32.2 d). Lambs were harvested at a commercial processor, hot carcass weight (HCW) was recorded, dressing percentage (DP) was calculated, and carcasses were chilled for 24 h. Carcasses were then ribbed and 12th rib backfat thickness (BFT), and loin muscle area (LMA) were measured. Traits were analyzed in mixed models with fixed class effects of finishing group (6 levels) and rearing type (single or multiple), a linear covariate of harvest age, and a random effect of sire (n = 16). There were no differences in BW, HCW, BFT, and LMA between rearing types but lambs reared as multiples had greater DP than those reared as singles (P = 0.02; Table). End BW increased while DP decreased with lamb harvest age (P ≤ 0.03). Pearson correlation coefficients were estimated between traits including post-weaning weight (PWWT) NSIP EBV. There was a strong, positive correlation between HCW and BFT (0.52) and HCW and BWT (0.54; P < 0.001). In contrast, HCW was only moderately correlated to LMA (0.35; P < 0.001). The PWWT EBV is the best genetic predictor of post-weaning lamb growth and was moderately correlated with end BW (0.38), HCW (0.35), and LMA (0.35; P < 0.001). Traditional markets prefer carcasses to be 29.5 – 36.3 kg HCW and < 6.4 mm BFT; 91% and 51% of Katahdin carcasses were below the HCW and BFT thresholds, respectively. Katahdins are capable of producing lean carcasses that conform to packer ideals for BF when fed to harvest BW of the present study, which may reduce excess fat trimming during fabrication. However, HCW observed in the present study may suggest greater suitability for non-traditional (i.e., ethnic) markets. Lower correlations between carcass traits in Katahdin compared with past research in Western white-faced lambs may highlight important biological differences in growth and fat deposition among divergent breed types. Crossbreeding Katahdin ewes with terminal sire breeds and within breed selection will improve growth and carcass traits without exceeding HCW thresholds commonly reached in wool sheep but should be considered in tandem with other breeding objectives.

Effect of Beef Silver Skin (Epimysium) Levels on Meat Emulsion Stability, Quality Attributes, and Texture Parameters.

The epimysium, also known as silver skin, is a fascia of connective tissue that surrounds each muscle. During fabrication, epimysium is removed from intact cuts, and it can be used as a source of collagen in processed meats to reduce production costs. However, little is known about the emulsifying properties of this collagen source. Thus, this study aimed to evaluate the effect of three levels of beef epimysium (silver skin, 0, 5, and 10%) on meat emulsion stability and on its cooked characteristics. Beef silver skin partially replaced ground beef, pork, and fat trimming, while all the other ingredients remained constant across formulations. The inclusion of silver skin did not affect (p > 0.05) chemical composition, total cooking loss, water loss, and raw emulsion color. Cooking fat loss linearly increased (p = 0.02) while cooked emulsion L* linearly decreased (p = 0.04) as silver skin level increased. Hardness, gumminess, and chewiness decreased linearly as silver skin levels increased (p < 0.01). Overall, incorporating silver skin into meat emulsions reduced stability, increased fat loss, and led to a weaker cooked emulsion matrix.

A Plant-Based Animal Fat Analog Produced by an Emulsion Gel of Alginate and Pea Protein.

As the market for plant-based meat analogs grows, the development of plant-based animal fat analogs has become increasingly important. In this study, we propose an approach by developing a gelled emulsion based on sodium alginate, soybean oil (SO), and pea protein isolate. Formulations containing 15% to 70% (w/w) SO were successfully produced without phase inversion. The addition of more SO resulted in pre-gelled emulsions with a more elastic behavior. After the emulsion was gelled in the presence of calcium, the color of the gelled emulsion changed to light yellow, and the formulation containing 70% SO exhibited a color most similar to actual beef fat trimming. The lightness and yellowness values were greatly influenced by the concentrations of both SO and pea protein. Microscopic images revealed that pea protein formed an interfacial film around the oil droplets, and the oil was more tightly packed at higher oil concentrations. Differential scanning calorimetry showed that lipid crystallization of the gelled SO was influenced by the confinement of the alginate gelation, but the melting behavior was like that of free SO. FTIR spectrum analysis indicated a potential interaction between alginate and pea protein, but the functional groups of SO were unchanged. Under mild heating conditions, gelled SO exhibited an oil loss similar to that observed in actual beef trims. The developed product has the potential to mimic the appearance and slow-rendering melting attribute of real animal fat.

Mineral composition of serially slaughtered Holstein steers supplemented with zilpaterol hydrochloride.

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, bone, internal cavity, hide, and fat trim components. Apparent mineral retention (Ca, P, Mg, K, and S) within the body was calculated as the difference between mineral concentration at slaughter and day 0. Mineral concentration at day 0 was determined from body composition of steers harvested at day 0 multiplied by individual live body weight (BW) at day 0. All data were analyzed as a 2 × 11 factorial arrangement with individual animal as the experimental unit. Orthogonal contrasts were used to analyze linear and quadratic contrasts over time (11 slaughter dates). There were no differences in concentration of Ca, P, and Mg in bone tissue as feeding duration increased (P ≥ 0.89); concentration of K, Mg, and S in lean tissue did fluctuate across DOF (P < 0.01). Averaged across treatment and DOF, 99% of Ca, 92% of P, 78% of Mg, and 23% of S present in the body were in bone tissue; 67% of K and 49% of S were in lean tissue. Expressed as gram per day, apparent retention of all minerals decreased linearly across DOF (P < 0.01). Expressed relative to empty body weight (EBW) gain, apparent Ca, P, and K retention decreased linearly as BW increased (P < 0.01) whereas Mg and S increased linearly (P < 0.01). Apparent retention of Ca was greater for CON cattle (greater bone fraction) and apparent retention of K was greater for ZH cattle (greater muscle fraction) when expressed relative to EBW gain (P ≤ 0.02), demonstrating the increase in lean gain by ZH cattle. There were no differences in apparent retention of Ca, P, Mg, K, or S due to treatment (P ≥ 0.14) or time (P ≥ 0.11) when expressed relative to protein gain. Apparent retention averaged 14.4 g Ca, 7.5 g P, 0.45 g Mg, 1.3 g K, and 1.0 g S/100 g protein gain. Expressing apparent mineral retention on a protein gain basis minimized effects of rate and type of gain, allowing for better comparison across treatments and time. Feeding zilpaterol hydrochloride did not affect apparent mineral retention when expressed relative to protein gain.

A comparative study of full-thickness skin grafting with and without subcutaneous fat preservation.

Skin grafting is an important method of wound repair and reconstruction. Skin grafting can be classified using multiple classification criteria. We often perform full-thickness skin grafting (FTSG) for small wound areas; however, the traditional FTSG technique frequently causes postoperative scar depression at the donor site, especially in the abdomen. This study aimed to determine whether preserving the subcutaneous fat when performing FTSG can improve donor site prognosis. We reviewed 25 patients who underwent autologous FTSG in the last 3 years. Among them, subcutaneous fat was preserved in 11 patients (experimental group), whereas it was not preserved in 14 patients (control group). Using a 3D camera and the Patient and Observer Scar Assessment Scale (POSAS), we evaluated the donor site postoperatively. According to POSAS, vascularization was significantly more severe in the experimental group. The Antera 3D camera revealed more severe scar depression at the donor site in the control group. The processing time for graft take, subcutaneous fat trimming and donor site closure was less in the experimental group than in the control group. Preserving subcutaneous fat at the donor site improved patient outcomes by reducing donor site depression after FTSG.

Effect of Different Selection Criteria on Performance, Carcass and Meat Quality of Nellore Young Bulls

This study was carried out to evaluate the effects of selection criteria for post-weaning daily gain (PWDG) and early sexual heifer precocity (PP14) on the performance, carcass traits and meat quality of Nellore bulls. In year one, 50 animals were selected according to their expected progeny differences (EPDs) for PWDG and grouped as high (HG) or low (LG) groups. In year two, 50 animals were selected according to EPD for PP14 and also grouped as high (HP) or low (LP). After slaughter, samples of the longissimus muscle area (LMA) were used to evaluate meat quality. Most of performance traits were not affected by the selection criteria. However, the HG group had higher dressing percentage (p = 0.028), LMA (p = 0.02) and fat trim in the forequarter (p = 0.04) compared to the LG group. The HP group tended to have greater dry matter intake (p = 0.08), LMA (p = 0.05), rump fat (p = 0.04), heavier striploins (p = 0.07), tenderloins (p = 0.09) and briskets (p = 0.08) compared with LP group. In conclusion, the selection based on divergent groups PWDG or PP14 has a small impact on performance, carcass and meat quality traits.

Selection for increased visual muscling increases carcass leanness without compromising predicted Meat Standards Australia eating-quality index

Context Selection using visual muscle score (MS) has been proposed to increase carcass leanness (i.e. meat yield), without compromising eating quality. Aims The aim of the present study was to examine the impact that selection for divergent MS has on live animal, commercial carcass and carcass tissue weights by using computed tomography (CT) including Meat Standards Australia (MSA) index-predicted eating quality. Methods Data from 67 steers originating from three muscling lines, namely, low, high and heterozygous high (HighHet – heterozygous for the 821 del11 myostatin mutation), were used. Visual MS was assessed on all steers. All steers were slaughtered and the left-hand side of each carcass was processed with fat trimming limited to only that required for hygiene purposes and kidney fat was not removed. All carcasses were MSA graded and then boned-out into untrimmed boneless primals (e.g. rump, cube roll). A CT scan of each beef primal was processed with image analysis software to estimate lean and fat tissue weights. The following traits were analysed: MS, weaning and slaughter weights; commercial carcass traits, including cold carcass weight, rump fat, MSA rib fat, MSA eye-muscle area, MSA marble score and MSA index; and CT-scanned compositional carcass traits, including lean, fat and bone tissues (%) and lean:bone ratio. All data were analysed with a linear mixed-effects model using REML. Least-squares means for the three muscling lines are reported. Linear trends between MS and seven carcass traits, with and without the myostatin mutation, are presented graphically. Key results Muscling line effects (P &amp;lt; 0.05) were found for visual MS and carcass traits. Linear trends between MS and carcass traits with and without the myostatin mutation demonstrate that increases in MS (P = 0.24) did not compromise predictions of MSA index even though MSA marble score decreased (P = 0.026), but myostatin decreased MSA marble score and tended to decrease MSA index (P = 0.097). Increases in the MSA eye-muscle area were associated with increases in MS (P &amp;lt; 0.01), with little effect of myostatin. Increases in MS and the myostatin mutation were both associated with increases (P &amp;lt; 0.01) in lean tissue (%) and the lean:bone ratio, and decreases (P = 0.02) in fat tissue (%). Conclusions The results indicate selection for high MS can be used to increase carcass yield, without negatively affecting MSA index predictions of eating quality. Implications Producers can use MS to identify animals with higher yields to increase carcass leanness and decrease carcass waste fat, without compromising MSA index predictions of eating quality, but should do so while considering all traits that affect profitability, in particular marble score and its association with eating quality.

Relationship between live body condition score and carcass fat measures in equine.

Relationships between live body condition score (BCS) and carcass fat depots have not been well established in equine. Our study was designed to quantify the relationship between BCS and fat depot measurements from equine carcasses. Live horses (n = 429) were evaluated immediately prior to immobilization at a commercial equine processor. Horses were independently assigned a BCS by a panel of three trained evaluators; BCS was evaluated by visual appraisal and manual palpation of the neck, withers, back, ribs, behind the shoulder, and tailhead. Median BCS frequencies were: 3.0 (n = 9), 4.0 (n = 43), 5.0 (n = 116), 6.0 (n = 86), 7.0 (n = 72), 8.0 (n = 76), and 9.0 (n = 27). Sex (stallion [n = 5], mare [n = 159], or gelding [n = 114]) and breed type (draft [n = 56], stock [n = 363], pony [n = 8], or mule [n =3]) were also denoted. Horses were processed for human consumption according to industry-accepted procedures under the supervision of the Canadian Food Inspection Agency. During the harvest process, all kidney–pelvic–heart (KPH) fat was trimmed from the carcass and weighed. After chilling, the marbling score was subjectively evaluated using beef grading standards. Carcass fat trim was weighed during the fabrication process. As BCS increased, hot carcass weight (HCW), absolute KPH weight, KPH expressed as a percentage of HCW, marbling score, neck fat depth, absolute weight of trimmed carcass fat, and trimmed carcass fat as a percentage of HCW increased (P < 0.01). A strong correlation (r = 0.74; P < 0.01) was detected between BCS and absolute KPH weight. Similarly, correlations between BCS and percentage of KPH (r = 0.65), neck fat depth (r = 0.60), absolute trimmed carcass fat (r = 0.58), trimmed carcass fat as a percentage of HCW (r = 0.54), marbling score (r = 0.54), and HCW (r = 0.52) were also detected (P < 0.01). These data indicate a strong relationship between subjective live BCS and objectively measured carcass fat depots in various equine breed types and sexes.

Prospects for the use of protein-carbohydrate complex based on mung bean seeds in the functional meat products technology

The use of new non-traditional types of plant materials in the diet is one of the ways to improve the meat products’ quality. The combination of plant proteins with animals creates biologically active amino-acid complexes which increase its bioavailability for organism and biological value of the product. In this case, the use of non-traditional plant materials in the production technology of new types of sausage products to improve the quality of the end product is prospective and relevant. Research objective is development of formulations and technologies for new types of high biological value products, based on combination of meat raw materials with functional additives of plant origin. The work was performed at the Department of Food Technology FSBEI HE «Don State Agrarian University», Persianovsky, Rostov region. The objects of research were the new protein-carbohydrate complexes based on flour from germinated seeds of mung bean, control and model forcemeat based on trim beef of first grade, lean trim pork and mid fat trim pork, and end products. Introduction to the cooked sausage products formulation of mung bean germinated seeds flour has a beneficial effect on the organoleptic properties of the end product and enriches it with biologically valuable substances.

Evaluation of feedlot performance, carcass characteristics, carcass retail cut distribution, Warner-Bratzler shear force, and fatty acid composition of purebred Jersey and crossbred Jersey steers.

Feedlot performance, carcass yield, fatty acid composition, and tenderness of crossbred Jersey steers compared with purebred Jersey steers was investigated. Purebred Jersey (n = 21) and crossbred Jersey steers sired by Angus (n = 9), SimAngus (n = 10), and Red Wagyu (n = 15) bulls were assessed. Adjusted to a common initial body weight (BW), crossbred Jersey steers had a greater rate of BW gain (P ≤ 0.01) compared with purebred Jersey steers. Angus sired steers had a greater daily dry matter intake (P ≤ 0.01) compared with Wagyu and Jersey sired steers, whereas SimAngus sired steers had a greater daily dry matter intake compared with Jersey sired steers. Wagyu sired steers were more feed efficient (P ≤ 0.03) compared with Jersey sired steers. Even with a greater (P ≤ 0.01) number of days on feed, off-test BW of purebred Jersey steers was less (P ≤ 0.01) compared with crossbred Jersey steers. Adjusted to a common hot carcass weight, Angus sired steers had a greater backfat thickness (P ≤ 0.01) compared with steers from the other sire breeds. Kidney fat percentage (P ≤ 0.01) was greatest for Jersey sired steers, with SimAngus and Wagyu sired steers being intermediate, and the lowest for Angus sired steers. Carcasses from Angus and Wagyu sired steers had a greater marbling score (P ≤ 0.03) compared with carcasses from Jersey sired steers. Carcasses from Wagyu sired steers had a greater (P ≤ 0.01) total red meat yield compared with Angus and Jersey sired steers, whereas SimAngus sired steers had a greater total red meat yield compared with Jersey sired steers. Carcasses from Angus sired steers tended (P = 0.07) to have a greater percentage of fat trim compared with Wagyu sired steer carcasses. There were no sire breed differences (P = 0.38) for the percentage of total bone from the carcasses. Tenderness, measured by Warner-Bratzler shear force (WBSF), was improved (P ≤ 0.01) with 14 d of postmortem aging compared with 7 d. Wagyu and SimAngus sired steers produced steaks with a lesser (P ≤ 0.01) WBSF compared with steaks from Angus and Jersey sired steers. Steaks from Angus sired steers tended (P = 0.10) to have a greater percentage of total lipid and had a greater (P ≤ 0.05) percentage of 16:0 compared with steaks from Jersey sired steers. Overall, crossbred Jersey steers improved economically relevant production parameters of feedlot performance, carcass quality, carcass yield, and instrumental predictors of eating quality compared with purebred Jersey steers.

Carcass fabrication yields of beef steers supplemented zilpaterol hydrochloride and offered ad libitum or maintenance energy intake.

An experiment was conducted to evaluate the fabrication yields of carcasses from beef steers supplemented zilpaterol hydrochloride (ZH) and fed at maintenance (MA) or ad libitum (AB) intake levels. Beef steers (n = 56) from a common sire were blocked (n = 28 per block) by terminal growth implant and sorted into pairs by BW. Four pairs (n = 8) were harvested on day 0; the remaining 24 pairs (n = 48) were assigned to a dietary intake level (MA or AB) and days on feed (28 or 56 d). Within pairs of MA or AB intakes, steers harvested on day 56 were randomly assigned to supplementation of ZH (90 mg·d−1 per steer) for 20 d followed by a withdrawal period of 4 d or control (C). Steers (BW = 603.5 ± 48.1 kg) were harvested at a commercial processing facility. After a 24-h chill period, standard USDA grading procedures were used to derive a calculated yield grade and quality grade. Following grading, left carcass sides were transported to the West Texas A&M University Meat Laboratory for fabrication. Each side was fabricated into subprimals to determine individual red meat yield (RMY), trimmable fat yield (TFY), and bone yield (BY). A mixed model was used for analysis; fixed effects included treatment combinations and random effects included block and pairs. Single df contrasts tested day 0 vs. 28, day 0 vs. 56, day 28 vs. 56, MA vs. AB, and C vs. ZH. Yield of chuck eye roll differed (P = 0.05) by days on feed (0 d = 4.14, 28 d = 4.11, 56 d = 4.55%). Similarly, eye of round yield was impacted (P = 0.02) by days on feed (0 d = 1.51, 28 d = 1.37, 56 d = 1.36%). Additionally, brisket yield was altered (P < 0.01) by days on feed (0 d = 4.08, 28 d = 3.56, 56 d = 3.48%) and treatment (C = 3.34, ZH = 3.61%). For remaining subprimals, no differences (P ≥ 0.15) were detected. Furthermore, results indicated that RMY tended (P = 0.07) to differ by treatment (C = 61.35, ZH = 63.67%). Comparatively, TFY was impacted (P = 0.04) by intake (MA = 20.44, AB = 23.33%). Results from this study indicate that a MA intake level during the last 56 d of the finishing period concurrent with ZH supplementation impacts subprimal yields as well as carcass RMY and TFY of beef steers.

United States beef quality as chronicled by the National Beef Quality Audits, Beef Consumer Satisfaction Projects, and National Beef Tenderness Surveys — A review

Meat quality is a very difficult term to define because it means different things to different people. When purchasing beef, consumers in the United States are likely to consider color, price, marbling level, subcutaneous fat trim, or cut thickness when determining the quality of beef. Once consumers have consumed the product, meat quality becomes exponentially more difficult to define due to the subjective nature of this term. Traditionally, tenderness, juiciness, and flavor have been considered the three most important factors that determine the palatability of beef. Therefore, American meat science beef research and industry focus has turned to measuring and quantifying these 3 attributes objectively and subjectively, and to determining what influences them. In reviewing the scientific literature, attempting to meaningfully summarize the findings of the thousands of studies on beef meat quality is impossible due to the inherent differences in the objective and methodology of studies. Fortunately, the United States beef industry and their national trade association, the National Cattlemen’s Beef Association (NCBA), have conducted numerous surveys and audits to characterize the quality of the products being produced and marketed by their cattlemen and the palatability perceptions of their consumers. The data produced by these studies is quite large and impossible to summarize in entirety in this review. Therefore, this review concentrates on the most important attributes that determine the value of a beef carcass and objectively measured and consumer-assessed palatability characteristics of fresh meat from these carcasses from 1987 through 2010.

Predictability of lean product, bone, and fat trim in beef carcasses from Costa Rica

Data from 292 hot fat trimmed carcasses derived from Costa Rican cattle were used to predict yield of fabricated boneless, closely-trimmed, high-valued cuts (BVS, by weight and percentage); yield of total saleable product (TSP, by weight and percentage); and percentage yields of bone and trim fat. Backfat thickness was not significantly associated with weight of BVS or TSP. Carcass weight explained 93.7% and 95.9% of the total variation in weight of BVS and TSP, respectively. Equations for predicting percentage yields of BVS and TSP showed little predictive efficacy. Conversely, the greater precision of the equations selected to predict the quantity (kg) of BVS or TSP, offers a practical alternative of using them in hot fat trimmed carcasses.

Effects of immunocastration and a β-adrenergic agonist on retail cuts of feedlot finished Nellore cattle

Immunocastration (ImC) has been proposed as an animal welfare-friendly alternative to reduce sexual and aggressive behavior and to increase carcass fat deposition with positive effects on meat quality. The β-adrenergic agonists (β-AA) are known as repartitioning agents that acts increasing lean tissue deposition. The combined use of these technologies can positively affect meat quality and increase retail cuts yield. Thus, this research was conducted to evaluate the combined effects of ImC and β-AA (zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH)) on retail cuts, bones, and fat trim of feedlot finished Bos indicus (Nellore) cattle. No interaction was observed between sexual condition and diet for any trait. The ImC decreased cold carcass, hindquarter (HQ), forequarter (FQ) and combined brisket, short ribs and flank (BSF) weights. The ImC also showed smaller weights of retail cuts and bones on the HQ and on the FQ than non-castrated (NoC). Fat trim weights did not differ from ImC and NoC. The most of subprimal cuts were heavier in NoC than in ImC. Feeding β-AA did not affect cold carcass weight; however, animals fed ZH had higher weights of HQ and retail cuts in HQ when compared with RH and control (CO) group, with no differences between RH and CO for both traits. The weights of FQ, BSF, retail cuts in FQ, as well as bones and fat trimmings were not affected by β-AA. In summary, ImC decreases carcass and retail cut weights, whereas ZH supplementation leads to an improvement in carcass lean tissue and retail cuts.

Consumer Acceptability and Willingness-To-Pay of Cooked Beef Bratwurst Sausage Formulated With Pre- or Post-Rigor Beef

ObjectivesThe current study was designed to determine consumer acceptability and willingness-to-pay (WTP) of 2 products, pre- and post-rigor beef Bratwurst sausages. Materials and MethodsFrom five 24-mo-old Holstein steers, the left chucks were deboned, ground (1.27-cm particle size), and salted (1.5% w/w) within 2 h post-mortem (pre-rigor treatment); whereas the right chucks were deboned at 72 h post-mortem (post-rigor treatment). On the day of sausage production (d 6 post-mortem), post-rigor trimmings were ground to 1.27-cm particle size and salted with 1.5% sodium chloride (w/w). Both pre- and post-rigor ground beef was processed into sausage batter by grinding the lean and fat trimmings separately through a 0.16-cm plate and mixing them with ingredients (beef bratwurst spice mix, water/ice slurry, corn syrup, erythorbate, nitrite, salt, and 0.25% w/w sodium tripolyphosphate). Sausages were cooked to 74°C by a generic smoked sausage cycle, including pre-drying, smoking, steaming, and cold shower, vacuum-packaged, and stored for 30 d at 2°C. Sausage links were warmed to 74°C and cut to 2.54-cm thickness. A total of 100 consumers evaluated appearance, aroma, flavor, texture, and overall acceptability on a 9-point hedonic scale. Each consumer was served with both pre- and post-rigor sausage from the same steer during each session. Immediately after the sensory evaluation, subjects were asked to formulate a WTP for each product using the information recorded on the evaluation sheets. A Becker–DeGroot–Marshak auction mechanism was used to solicit truthful WTPs. An aggregate unit-demand curve was constructed from the entire subject pool’s bidding data. Cluster analysis was conducted by the Agglomerative Hierarchical Clustering procedure in XLSTAT 2018.2.50198 using Ward’s method. Consumer acceptability was subsequently analyzed in each cluster by the GLIMMIX procedure of SAS 9.4 (SAS Inst. Inc., Cary, NC) at 0.05 level of significance. ResultsCluster analysis revealed 5 consumer clusters based on overall acceptability. Cluster 1 (7.9 and 8.1 overall acceptability unit for pre- and post-rigor, respectively; P = 0.130) and 5 (3.9 and 3.4 for pre- and post-rigor, respectively; P = 0.340) consisted of 40 consumers and had no difference in any sensory attribute between pre- and post-rigor treatments (P ≥ 0.091). However, panelists in cluster 3 (37 consumers) preferred the aroma, flavor, and texture of pre-rigor than post-rigor sausage (P ≤ 0.011). Similarly, 8 consumers in cluster 4 preferred the flavor, texture, and overall acceptability of pre-rigor than post-rigor sausage (P ≤ 0.020). However, 15 consumers in cluster 2 liked the texture of post-rigor sausage better (P = 0.004). Average predicted demand was 0.7 lb greater for pre-rigor than post-rigor sausage (P ≤ 0.001). In a scenario with a realistic market price of $6/lb, demand for pre-rigor sausage was 5% greater than post-rigor sausage. This greater demand for pre-rigor sausage might be driven by the greater acceptability of flavor and texture found in cluster 3 and 4. ConclusionPre-rigor processing yields sausage with greater consumer acceptability and demand. However, scalable production costs should be considered before finalizing the decision to implement pre-rigor processing in an industrial setting.

Allometric coefficients for carcass and non-carcass components in a local meat-type sheep breed

The aim of the research was to assess the type of development of carcass and non-carcass components and tissue partitioning in the Cornigliese sheep breed. The study involved 164 animals (104 females, 60 males) from one flock. The selection of those animals accounted for ages and weights differences. Fasted body weight (BW) was recorded before slaughtering. Four BW-classes were identified by means of percentiles distribution: 0–25th percentile, 15.90–50.70kg BW; 25th–50th percentile, 50.71–63.22kg BW; 50th–75th percentile, 63.23–75.50kg BW; 75th–100th percentile, 75.51–111.15kg BW. After slaughter, the carcasses were weighed, measured (carcass length, croup width, chest width, chest depth, thigh length) and sectioned (shoulder, neck, thigh, ribs, loin, and lean and fat trimmings). A samplecut from 1st to 4th thoracic vertebra was then separated into its tissue components (lean meat, fat, bone). Non-carcass components (skin+fleece, head, liver, heart, lungs+trachea) were weighed. The data were analyzed using a GLM procedure accounting for the following fixed effects: sex, BW-class, year of birth and the interaction between sex*BW-class and the covariate: day of slaughter. The relative growth of carcass and non-carcass components on BW was calculated by applying the nonlinear allometric function (y=axb). We found high coefficients of variation (CVs) (from 39% to 77%) for the weight of carcass components and low CVs (from 8% to 22%) for carcass measures. Non-carcass parameters showed intermediate values of CVs (from 30% to 42%). Body weight-class had a significant effect (P<0.05) on all parameters, except on lean/fat ratio and lean percentage of the sample cut. Significant mean differences between genders were observed in the lean/fat ratio. In particular, in the lowest BW-class, females scored higher than males, whereas, in the highest BW-class, we observed the opposite trend (P<0.05). Carcass, loin, lean and fat trimmings grew faster than BW, regardless of the sex. In contrast, as far as the neck is concerned, significant differences between females and males were observed (P<0.05). Among carcass measures, a late maturing rate was recorded for croup width (b=0.5042) and, in this case, females tended to be earlier than males (P<0.10). Among non-carcass parameters, only head and skin+fleece weights showed differences between sex groups, with males showing a later development than females (P<0.05). Tissues (lean, fat, bone) in the sample cut developed with different speed: the earliest tissue was bone (b=−0.3191), followed by lean (b=0.0768) and finallyby fat (b=0.8394). Bone development was significantly (P<0.05) sex related, with males showing a later growth than females. Sex differences in the sample cut composition were also associated to lean/fat, (lean+fat)/bone and lean/bone ratios. This study has shown the potential use of the Cornigliese sheep as a commercial breed for meat production, although significant interaction between sex and body weight was observed. For this reason, it is recommended to implement a breeding strategy to reduce the incidence of extreme fattening females and the high incidence of non-carcass component in males when they reach high BWs.

Fabrication yields of serially harvested calf-fed Holstein steers fed zilpaterol hydrochloride1

Holstein steers ( = 110) were fed zilpaterol hydrochloride (ZH) for 0 or 20 d before slaughter during a 280-d serial harvest study. Cattle were harvested every 28 d beginning at 254 d on feed (DOF) and concluding at 534 DOF. After slaughter, carcasses were chilled for 48 h and then fabricated into boneless closely trimmed or denuded subprimals, lean trim, trimmable fat, and bone. Inclusion of ZH increased cold side weight (CSW) by 10.3 kg ( < 0.01; 212.7 vs. 202.4 kg [SEM 1.96]) and saleable yield by 10.4 kg ( < 0.01; 131.9 vs. 121.5 kg [SEM 1.16]) in calf-fed Holstein steer carcasses. Additionally, saleable yield as a percentage of CSW increased ( ≤ 0.01) by 2.19% (62.64 vs. 60.45% [SEM 0.22]) for cattle supplemented with ZH. Subprimal weights were heavier ( ≤ 0.05) from cattle that received ZH except for the bottom sirloin ball tip, back ribs, and outside skirt regardless of slaughter endpoint. Yield of top round, bottom round, and knuckle was increased ( ≤ 0.01) following ZH supplementation by 0.37, 0.24, and 0.18%, respectively. Yield of the top sirloin butt, strip loin, and tenderloin was increased ( ≤ 0.01) concurrent with ZH supplementation by 0.18, 0.11, and 0.09%, respectively. Regarding the rib primal, the rib eye roll tended ( = 0.08) to had increased yield (2.80 vs. 2.72% [SEM 0.03]) with ZH supplementation; both back ribs and blade meat exhibited increased ( ≤ 0.04) yields of 0.04%. Relative to the chuck primal, increased ( ≤ 0.03) yields of shoulder clod, pectoral meat, and mock tender were observed (0.13, 0.07, and 0.04%, respectively). Yield changes for subprimal brisket, plate, and flank were limited to increased ( < 0.01) proportion of flank steak and elephant ear (cutaneous trunci), 0.07 and 0.04%, respectively. Feeding duration notably altered ( ≤ 0.01) weights and percentages of all subprimals except the brisket. Saleable yield increased ( ≤ 0.01) by 0.192 kg/d with additional DOF. Moreover, trimmable fat and bone increased ( ≤ 0.01) by 0.146 and 0.050 kg/d, respectively. These data illustrate improved saleable meat yields for calf-fed Holstein steers supplemented with ZH and provide the beef industry knowledge of fabrication yield changes throughout a wide range of harvest endpoints.

Effect of early post-hatch nutrition on production performance of commercial broiler chicken

The study was conducted to access the effect of early post-hatch nutrition regimens on the productive performance of 384 number of commercial broiler chicken (Cobb 400). Different nutrient regimens include glucose and probiotic alone in hatcher tray and first day in pen and a high and very high density diet of 25 and 27 per cent dietary crude protein respectively and 3100 kcal ME/kg diet in hatcher tray and subsequent three days in pen along with glucose and probiotic alone in hatcher tray and first day in pen was provided. Later, all broiler chicks were switched over to the recommended commercial Cobb broiler diet up to 42 days. Body weight, feed efficiency and breast muscle fibre diameter were significantly higher in treatment groups. No significant difference existed between different treatment groups for hatchability, livability percent, carcass characteristics and cut up parts. Trimmable fat per cent was significantly higher (P less than 0.01) in control birds. It may be concluded that early post hatch nutrition with high nutrient dense diet resulted in better production performance in commercial broiler chicken.

Fatty acids in beef from grain- and grass-fed cattle: the unique South African scenario

Objective: Different fatty acids elicit different responses in the human body once ingested. Although red meat is often considered to be a source of fatty acids which has a negative impact on human health, many studies have reflected variability in the quantity and quality of fatty acids found in red meat produced on different production systems in different countries. This study evaluated the fatty acid profile of beef, produced by the grass- and grain-fed production systems practised in South Africa.Design: Data are reported as a percentage of lipid per 100 g total fat to enable a comparison with international findings. Furthermore, the findings are translated into edible meat portions, taking fat trimming (often associated with red meat intake) into consideration in order to determine the contribution which the different products can make to the human diet.Subjects and setting: Three cuts of beef from cattle from four production groups were sampled and the fatty acid composition analysed for the meat and fat fractions.Results: Notable differences were found in the quantity and quality of different fatty acids in beef from the different production systems. When untrimmed, no statistically significant difference was found in the total fat between beef produced on the different production systems. Differences became more significant as trimming was performed. When trimmed of all visible fat, beef from young cattle fed according to a grain-based feeding system contained less total fat (6.96 g), and less saturated fat (2.16 g) per 100 g, than beef produced from their grass-fed counterparts (9.77 g and 3.30 g, respectively). There was a more favourable omega-6 to omega-3 fatty acid ratio, i.e. 2.0–2.5:1.0 for grass-fed cattle, compared to 8–30:1 for grain-fed cattle, irrespective of the degree of trimming. The beef from the grass-fed cattle also contained a higher quantity of conjugated linoleic acid.Conclusion: A unique classification system for red meat has been implemented in South Africa and dictates the characteristics of the fresh meat that is available to consumers. The results of this study consequently indicate distinctive differences between the fatty acid profile of local red meat and that of beef produce from other countries; often used as a reference for dietary guidance.