Fat Samples Research Articles

PSXI-21 Inclusion of Former Food Products in Post-Weaning Diet of Piglets: Impact on the Fatty Acid Profile of Subcutaneous Adipose Tissue and on Serum Metabolites

Abstract Livestock production is facing the feed-food competition, use of natural resources, and environmental sustainability. To address these issues, the use of food leftovers in animal nutrition could satisfy the principles of circular economy. Among the different food leftovers, former food products (FFPs) consist mainly of unsold/defective food from industrial production (i.e., bread, croissants, biscuits, chips). FFPs can be classified in sugary confectionary FFPs (FFPs-C) and salty bakery FFPs (FFPs-B). The aim of this study was to assess the impact of the inclusion of FFPs into post-weaning diet of piglets on the fatty acid profile of subcutaneous adipose tissue and on serum metabolites. Thirty-six post-weaning female piglets (Large White×Landrace, BW 8.52±1.73kg) were assigned to a standard diet (CTR), or with a 30% replacement (wt/wt) of conventional cereals with FFPs-C or FFPs-B for 42 days. Growth performance and feeding behavior were measured. Subcutaneous abdominal fat samples were collected from 12 selected piglets after slaughtering. Gas chromatography was performed on feed samples from control diet and experimental diets and on adipose tissue samples. Untargeted metabolomics was performed on blood samples (d 0 and d 42) using UHPLC/MS-MS. Data were analyzed with the software MetaboAnalyst (version 5.0). The inclusion of FFPs did not alter growth performance or feeding behavior. The CTR diet had greater PUFA concentrations than FFPs diets. In FFPs diets, the fatty acid profile was equally distributed among SFA, MUFA, and PUFA. The ω-6/ω-3 ratio was greater in FFPs-C diets than CTR and FFPs-B diets. The subcutaneous adipose tissue showed no differences between groups for SFA concentrations. The MUFA concentrations were reduced in CTR compared with FFPs groups, whereas PUFA concentrations were reduced in FFPs-C group compared with FFPs-B and CTR groups. The ω-6/ω-3 ratio was less in FFPs-C group compared with CTR and FFPs-B groups. The untargeted metabolomics identified a total of 104 metabolites, with 2 significantly different between groups. Specifically, the serum concentration of theobromine and caffeine was greater in FFPs groups compared with CTR. To conclude, piglets were able to rebalance the fatty acids profile despite the differences observed in the diets. The metabolic status was not strongly altered by the inclusion of 30% FFPs in the diet. Thus, FFPs could be safely applied in post-weaning pig diets. Further studies are needed to assess the effect of FFPs on pig physiology and metabolic status in growing phase pigs and for a longer feeding time. (funded by ASSO Project- Regione Lombardia)

240 Comparing Increasing Tryptophan: Lysine Ratios in Distiller Dried Grains-Based Diets with or without Distiller Dried Grains withdrawal Strategy on Growth and Carcass Performance of Grow-Finish Pigs

Abstract A total of 6,240 pigs [DNA 600 × PIC 1050; initial body weight (BW) = 22.5 ± 0.20 kg], divided into 2 groups, were used in 119- or 120- day study comparing increasing Trp:Lys ratio in diets with DDGS or a DDGS withdrawal strategy on growth and carcass performance and carcass fat iodine value of grow-finish pigs. Pigs were randomly allotted to 1 of 7 dietary treatments with 30 to 36 pigs per pen and 26 replications per treatment. Diets were fed in 4 phases (approximately 23 to 44, 44 to 71, 71 to 104, and 104 kg to market). Diets included a control corn-soybean meal-based diet formulated to a 19% SID Trp:Lys ratio, 4 diets with 30% DDGS fed in all four phases and formulated to achieve 16%, 19%, 22%, or 25% SID Trp:Lys ratio, respectively, and 2 DDGS withdrawal strategy diets: 19% SID Trp:Lys with 30% DDGS in phase 1 through 3 and then in phase 4, 0% DDGS with either a 19 or 25% Trp:Lys ratio. Overall, BW, ADG, ADFI, and G:F increased (linear, P < 0.05) as SID Trp:Lys ratio increased in diets with 30% DDGS fed in all 4 phases (Table). Pigs fed diets containing a SID Trp:Lys ratio of 19% and 30% DDGS from phases 1 through 3 and 0% DDGS in phase 4 had the greatest numeric ADG and ADFI for the overall study, but was not different than pigs fed the control for ADG, or the 25% Trp:Lys withdrawal treatment and the 30% DDGS diets with 25% Trp:Lys ratio throughout the study for ADG and ADFI. Hot carcass weight and carcass yield increased (quadratic, P < 0.05) as Trp:Lys ratio increased. Carcass fat samples were taken from the loin-butt junction and analyzed for iodine value. Pigs fed the control diet had decreased (P < 0.05) carcass fat iodine value compared with pigs fed DDGS throughout the study, with pigs fed the two DDGS withdrawal strategies having decreased (P < 0.05) iodine values compared with pigs fed 30% DDGS in all 4 phases. In summary, increasing the SID Trp:Lys ratio in diets with 30% DDGS resulted in a linear improvement in ADG, ADFI, G:F, and BW but did not influence iodine values. Removing DDGS from the diet in the last period reduced carcass fat iodine value and increased growth rate during the withdrawal period compared with pigs fed 30% DDGS throughout, indicating value in a DDGS withdrawal strategy.

Modifying Headspace Sampling Environment Improves Detection of Boar Taint Compounds in Pork Fat Samples

The extraction of boar taint compounds from pork fat samples was performed under various temperature (150, 300 and 450 °C) and atmosphere (air, nitrogen and reduced pressure) conditions. This aimed at understanding which conditions allow the greatest extractions of indole, skatole and androstenone (present in backfat in low concentrations) while limiting the presence of other VOCs in the headspace of heated fat (interfering with correct VOC-based detection of boar taint compounds). Indole and skatole were extracted in the greatest concentrations when heating backfat at 450 °C under reduced pressure, while androstenone was highest when heating at 300 °C under reduced pressure. Oxidation products were most abundant under air conditions, nitrogenated products appeared in the presence of a nitrogen-enriched atmosphere, and lastly, molecules intrinsic to boar fat saw their headspace concentration increase with reduced pressure. The combination of 450 °C and reduced pressure atmosphere was suggested for the heating of backfat prior to detection with analytical methods and to complement the current sensory analysis.

Polar pesticides in food of animal origin: interlaboratory validation to evaluate method fitness-for-purpose of official control

The present work reports on the design, execution and evaluation of results of an interlaboratory validation study aimed at verifying the fitness-for-purpose of a LC-MS/MS method for the detection of polar pesticides in food of animal origin in official control and monitoring programmes. To this scope, five participant laboratories, with relevant expertise, were recruited. After passing a pre-trial test, the participants were asked to analyse test samples of bovine fat, chicken eggs and cow’s milk, contaminated with 11 polar pesticides (group A: Aminomethyl phosphonic acid (AMPA), cyanuric acid, ethephon, glyphosate, fosetyl aluminium, 2-hydroxyethyphosphonic acid (HEPA), maleic hydrazide, N-acetyl-glyphosate, group B: N-acetyl glufosinate (NAG), 3-methylphosphinicopropionic acid (MPP) and glufosinate ammonium) at two different levels (0.05 and 0.25 mg/kg−1 and 0.01 and 0.05 mg/kg−1 for group A and B respectively. The method was based on acidified methanol/water extraction followed by dSPE clean up with C18 sorbent. For LC-MS/MS analysis isotopically labelled standards were used for all targeted analytes. With a couple of exceptions, average recoveries ranged from 85% to 110%, with repeatability (RSDr) ranging from 3% to 25%, and reproducibility (RSD R ) from 4% to 26%. The assessment by different laboratories provided also insights on key factors impacting method performance characteristics and its implementation by new users.

Adipose tissue composition determines its computed tomography radiodensity.

Adipose tissue radiodensity in computed tomography (CT) performed before surgeries can predict surgical difficulty. Despite its clinical importance, little is known about what influences radiodensity. This study combines desorption electrospray ionization mass spectrometry imaging (DESI-MSI) and electrospray ionization (ESI) with machine learning to unveil how chemical composition of adipose tissue determines its radiodensity. Patients in the study underwent abdominal surgeries. Before surgery, CT radiodensity of fat near operated sites was measured. Fifty-three fat samples were collected and analyzed by DESI-MSI, ESI, and histology, and then sorted by radiodensity, demographic parameters, and adipocyte size. A non-negative matrix factorization (NMF) algorithm was developed to differentiate between high and low radiodensities. No associations between radiodensity and patient age, gender, weight, height, or fat origin were found. Body mass index showed negative correlation with radiodensity. A substantial difference in chemical composition between adipose tissues of high and low radiodensities was observed. More radiodense tissues exhibited greater abundance of high molecular weight species, such as phospholipids of various types, ceramides, cholesterol esters and diglycerides, and about 70% smaller adipocyte size. Less radiodense tissue showed high abundance of short acyl-tail fatty acids. This study unveils the connection between abdominal adipose tissue radiodensity and its chemical composition. Because the radiodensity of the fat around the surgical site is associated with surgical difficulty, it is important to understand how adipose tissue composition affects this parameter. We conclude that fat tissue with a higher content of various phospholipids and waxy lipids is more CT radiodense. This study establishes the connection between the CT radiodensity of adipose tissue and its chemical composition. Clinicians may use this information for preoperative planning of surgical procedures, potentially modifying their surgical approach (for example, performing partial nephrectomy openly rather than laparoscopically). • Adipose tissue radiodensity values in computed tomography images taken prior to the surgery can potentially predict surgery difficulty. • Fifty-three human specimens were analyzed by advanced mass spectrometry, molecular imaging, and machine learning to establish the key features that determine Hounsfield units' values of adipose tissue. • The findings of this research will enable clinicians to better prepare for surgical procedures and select operative strategies.

Proton transverse relaxation times depending on the unsaturated fatty acids: a magnetic resonance relaxometric study on beef fat samples

ABSTRACT The unsaturated fatty acid content of beef meat is an important property that affects the health and sense of taste of the consumer and determines price. Fresh beef fat samples were measured by a bilateral low-field time-domain proton magnetic resonance (MR) apparatus. The proton transverse relaxation time (T2) for 18 fat samples were measured at 40.0°C using the Carr-Purcell-Meiboom-Gill method. The results showed that T2 increased linearly with unsaturated fatty acid content. By using the linear dependence of T2 on the unsaturated fatty acid content, we successfully estimated the contents of oleic acid, total monounsaturated fatty acid, and total unsaturated fatty acid with root-mean-square errors of 2.5, 2.8, and 3.2 wt%, respectively. The proposed T2-relaxometry-based method could be performed using a portable unilateral MR surface scanner, which would enable in-vivo nondestructive noninvasive measurements of live cattle for the optimization of the feeding and fattening timing from calf to adult and for the best choice of the shipment timing in terms of the maximization of unsaturated fatty acid content of beef meat.

Decoding the role of triacylglycerol composition in the milk fat crystallisation behaviour: A study using buffalo milk fat fractions

The role of triacylglycerols (TAGs) composition in milk fat crystallisation is evaluated from a new perspective by correlating the crystal structures and their predicted TAG contributors. Four different TAG groups, defined by their fatty acid (FA) chain length and saturation are proposed to explain the crystallisation behaviour of different milk fat samples, namely TAGs containing (i) fully saturated long chain FAs, (ii) single unsaturated long-chain FA and (iii) multi unsaturated long-chain FAs, as well as (iv) short-chain FAs. Samples containing different TAGs proportions were obtained from two-step dry fractionation of buffalo milk fat. Liquid chromatography and mass spectrometry (LCMS) was employed to determine the TAGs composition. The crystallisation characteristics were evaluated using small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) and polarised light microscopy. The results confirm that fully saturated long chain TAGs are responsible for the formation of high melting point 2L-crystals (d-spacing: 44.1–49.7 Å), whilst the low melting point 3L-crystals (64.4–72.8 Å) are mainly contributed by long chain TAGs containing unsaturated fatty acids. Further, the crystallisation inhibiting effect of asymmetrical TAGs, containing short chain fatty acid (C4-6) is evident. As a final point, effects of the four TAG groups, each given with a specific composition, were examined closely also with respect to their influence on the nucleation and polymorphic transformation kinetics of milk fat crystals.

Novel approach for determination of milk fat adulteration with non-milk fat by RP-HPLC

The present work studied the method for quickly determining milk fat adulteration with non-milk fat. The approach was based on analyzing characteristic fingerprints of triacylglycerols profile in combination with chemometric techniques and a multidimensional vector model. Nineteen fat samples of bovine milk, 20 samples of cow cheese, and ten samples of cow butter were analyzed by reverse-phase high-performance liquid chromatography. The areas of 3 triacylglycerol peaks with equivalent carbon numbers of 35.6, 46.2, and 47.2 were used for diagnosing chemometrics and building the vector model. It was shown that the proposed approach could be used to quickly determine milk fat adulteration by non-milk fat with a concentration from 1.5 to 100%. Application of the vector model to 20 samples of cow cheese showed that most of them were made of authentic cow milk; however, the milk fat in 7 of these samples had been replaced with non-milk fat. For ten samples of cow butter, only three samples were produced from milk fat alone, while for other samples, the mass fraction of non-milk fat, expressed as palm oil, was determined to be in the range of 10–90%.

Normal diet ameliorates obesity more safely and effectively than ketogenic diet does in high-fat diet-induced obesity mouse based on gut microbiota and lipid metabolism

Growing evidence supports the efficacy of ketogenic diets for inducing weight loss, but there are also potential health risks due to their unbalanced nutrient composition. We aim at assessing relative effectiveness of a balanced diet and ketogenic diet for reversing metabolic syndrome in a diet-induced C57BL/6J mouse model. Mice were fed high-fat diet to induce obesity. Obese individuals were then fed either ketogenic or balanced diets as an obesity intervention. Serum, liver, fat and faecal samples were analysed. We observed that both diet interventions led to significant decrease in body weight. The ketogenic intervention was less effective in reducing adipocyte cell size and led to dyslipidaemia. The composition of the gut microbiome in the balanced diet intervention was more similar to the non-obese control group and had improved functional attributes. Our results indicate intervention with balanced diets ameliorates obesity more safely and effectively than ketogenic diets in diet-induced obesity mouse model.

The role of microbial metabolites in diabetic kidney disease

BackgroundGrowing evidence suggests a complex bidirectional interaction between gut microbes, gut-derived microbial metabolites, and diabetic kidney disease (DKD), known as the “gut-kidney axis” theory. The present study aimed to characterize the role of microbial metabolites in DKD. MethodsSix-week-old db/db and littermate db/m mice were raised to 20 weeks old. The serum, urine, feces, liver, perinephric fat, and kidney were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS)-based metabolomic analyses. ResultsThe db/db mice showed obvious pathological changes and worse renal functions than db/m mice. Indoleacetaldehyde (IAld) and 5-hydroxy-l-tryptophan (5-HTP) in kidney samples, and serotonin (5-HT) in fecal samples were increased in the db/db group. Phosphatidylcholine (PC), phosphatidate (PA), and 1-acylglycerophosphocholine (lysoPC) were decreased in liver and serum samples of the db/db group, while PC and lysoPC were decreased in kidney and perinephric fat samples. Suggested metabolomic homeostasis was disrupted in DKD mice, especially glycerophospholipid and tryptophan metabolism, which are closely related to the gut microbiome. ConclusionsOur findings reveal the perturbation of gut microbial metabolism in db/db mice with DKD, which may be useful for building a bridge between the gut microbiota and the progression of DKD and provide a theoretical basis for the intestinal treatment of DKD.

Exogenous lactate intake immediately after endurance exercise increases time to exhaustion in VO2max measurements in mice.

The purpose of the study was to investigate the effects of 4 weeks of lactate intake immediately after endurance exercise on maximal oxygen uptake (VO2max) in exercise performance. Seven-week-old mice from the Institute of Cancer Research (ICR) were randomly divided into four groups: vehicle intake (SE/CON), lactate intake (SE/LAC), endurance exercise with vehicle intake (EX/ CON), and lactate intake with endurance exercise (EX/ LAC). Mice were subjected to 60-70% VO2max endurance exercise with or without oral lactate intake 5 days/ week for 4 weeks. VO2max measurements (VO2max, time to exhaustion (TTE), respiratory exchange rate, fat oxidation, and carbohydrate oxidation) were recorded at the end of the study period. After 48 h of VO2max measurement, the mice were sacrificed, and three different abdominal fat samples (epididymal, perirenal, and mesenteric) were collected. Body weight and abdominal fat mass did not differ between the groups. When measuring VO2max, endurance exercise raised VO2max, and lactate intake after endurance exercise increased TTE. The change in energy substrate utilization during VO2max measurement demonstrated that although the respiratory exchange rate and fat oxidation were enhanced by lactate intake, there were no synergistic effects of lactate intake and endurance exercise. Lactate intake immediately after endurance exercises can improve exercise performance, indicating the benefit of long-term exogenous lactate intake as an exercise supplement.

#4362 A DISTINCT TRANSCRIPTOME SIGNATURE IN HUMAN RENAL SINUS ADIPOSE TISSUE – A POTENTIAL IMPORTANCE FOR RENAL DYSFUNCTION?

Abstract Background and Aims Previous studies have suggested that the anatomical location and function of adipose tissue (AT) is of relevance for normal as well as impaired renal function. Renal sinus AT (RSAT) is a perivascular fat compartment located around the renal arteries. The aim of this study was to perform a transcriptomic characterization of RS in comparison with omental (OAT) and subcutaneous (SAT) AT in healthy individuals. Method 30 kidney donors (15 men (50%), age 50 ± 11 years, BMI 25.8 ± 2.9 kg/m2, eGFR 83 ± 9 mL/min/1.73 m2, HOMA-IR 2.5 ± 1.1) were included in the study. RS, OAT and SAT biopsies were performed during laparoscopic unilateral nephrectomy. RNA-sequencing analyses and untargeted transcriptomics were performed. Analysis of differentially expressed genes (DEG) was employed to discover quantitative changes in expression levels between two tissue depots (RSAT) vs OAT and SAT, respectively. Gene Ontology (GO) term enrichment was used to summarize the function of genes that were differentially expressed. Results Paired samples of renal sinus fat (RSAT) to be compared with omental (OAT) or subcutaneous adipose tissue (SAT) were available in 26 and 22 subjects, respectively. Out of 12210 genes characterised in these AT depots, 463 genes (3,7%) were uniquely expressed in RS when compared to both OAT and SAT (co-expression Venn-diagram in figure 1). In addition, DEG analyses showed that 1639 genes had higher, whereas 384 had lower, expression in RS than in OAT. For RSAT versus SAT the corresponding numbers were 3789 and 2534, respectively. Among the gene that were differentially regulated, the following pathways were most significantly enhanced in RSAT compared to OAT or SAT (figure 2): chemotaxis and cell migration (e.g. cell and leukocyte chemotaxis and leukocyte migration), and immune response (e.g. T-cell activation, immune response-activating signal transduction, adaptive immune response). Conversely, ATP synthesis (e.g. oxidative phosphorylation, cellular respiration, mitochondrial complex) and extracellular matrix pathways were significantly attenuated in RS compared to SAT and OAT, respectively (not shown). A targeted analysis of specific gene markers that have previously been reported to be associated with chronic kidney disease (CKD) showed that AT expression of pro-inflammatory factors such as visfatin, IL-6, IL-11, IL-1β and IL-18 were higher in RSAT vs SAT or OAT (not shown). In addition, the expression of some genes, differently regulated in RSAT, and known to associated with progression of CKD, e.g. Fc Gamma Receptor IIa (FCGR2A), NF-kappa-B inhibitor beta (NFKBIB), Nuclear Factor, Erythroid 2 (NFE2) and Oncostatin M (OSM), were positively correlated with BMI (data not shown). Conclusion This study in healthy kidney donors indicates that the transcriptome signature of renal sinus AT seems to be distinct from subcutaneous and omental AT. Gene ontology analyses pointed towards an inflammatory environment and lower mitochondrial function in the renal sinus compared to other adipose tissue depots. These findings are likely to define depot-specific AT functions that may be of importance for renal function and disease.

Microbiological safety criteria for products from unconventional raw materials: raw bear fat

Fat of game animals is a prospective raw material for bioactive additives. Before such a product enters the market, food science has to make sure it is safe for consumption.
 This research featured subcutaneous adipose tissue of brown bears tested with standard methods for microbiological safety indicators. The microbial properties were studied on liquid and solid nutrient media. Staphylococcus was profiled using a VITEK 2 Compact biochemical automatic analyzer and Gram-positive cards (Bio-Mérieux, France). The analysis followed the Technical Regulations of the Customs Union TR TS 021/2011 On Food Safety (December 09, 2011).
 The microbial count for mesophilic aerobic and facultative anaerobic microorganisms was 1.5×103 CFU/g. The fat samples revealed no molds, yeasts, or Escherichia coli bacteria. Liquid and solid nutrient media made it possible to describe the qualitative profile and cultural properties of the bear fat microflora against pork fat, which served as control. The automatic system identified Gram-positive, coagulase-negative, and oxidase-positive Staphylococcus lentus and Staphylococcus sciuri. In line with the modern classification, they belong to the new genus of Mammaliicoccus, namely Mammaliicoccus sciuri.
 Subcutaneous adipose tissue of brown bears needs to undergo a microbiological safety test before consumption. Bear fat requires additional research in order to become a safe raw material for food products and bioactive additives.

Pathophysiologic Contributions of Visceral Adiposity to Left Ventricular Diastolic Dysfunction.

Visceral fat produces inflammatory cytokines and may play a major role in heart failure with preserved ejection fraction (HFpEF). However, little data exist regarding how qualitative and quantitative abnormalities of visceral fat would contribute to left ventricular diastolic dysfunction (LVDD). We studied 77 participants who underwent open abdominal surgery for intra-abdominal tumors (LVDD, n = 44; controls without LVDD, n = 33). Visceral fat samples were obtained during the surgery, and mRNA levels of inflammatory cytokines were measured. Visceral and subcutaneous fat areas were measured using abdominal computed tomography. Patients with significant LVDD had greater LV remodeling and worse LVDD than controls. While body weight, body mass index, and subcutaneous fat area were similar in patients with LVDD and controls, the visceral fat area was larger in patients with LVDD than in controls. The visceral fat area was correlated with BNP levels, LV mass index, mitral e' velocity, and E/e' ratio. There were no significant differences in the mRNA expressions of visceral adipose tissue cytokines (IL-2, -6, -8, and -1β, TNFα, CRP, TGFβ, IFNγ, leptin, and adiponectin) between the groups. Our data may suggest the pathophysiological contribution of visceral adiposity to LVDD.

Prototype for rapid test devices to detect residues of sulfonamides in chicken carcasses from traditional breeders in Surabaya, Indonesia.

Sulfadiazine, one of the sulfonamide group's active compounds, is widely used for therapeutic production against several diseases. Veterinary drug residues can have a significant impact on human health conditions. This study aimed to develop a prototype of rapid test devices (RTDs) for detecting sulfadiazine residues on chicken carcasses based on the color indication. Seven samples of carcasses collected from traditional breeders in Surabaya-Indonesia were prepared and tested using RTDs. This sample represents the population considering that in the last report, the use of antibiotics was more than 40%, while the ability to monitor RTDs was estimated at 100. The standard color of purple by Hex code standard color or decimal code color was used to compare the positive samples. A light-emitting diode (LED) lamp was used to observe purple color. Analysis of sulfonamides resulting from RTDs was compared using a ultraviolet-visible spectrophotometer. Sulfonamides contamination levels of 50% and 100% were detected at concentrations of 0.472 μg/mL and 0.642 μg/mL, respectively. Sulfonamides contamination that was <0.395 μg/mL did not appear purple. The study's findings showed that RTDs can be used to detect sulfonamides residues at a limit of detection 0.5 mg/mL after a 45 min exposure to an LED operating at a wavelength of 980 nm (p < 0.05). The limitation of RTDs was not being able to monitor the presence of residues bound in fat samples. Rapid test devices can be developed for commonly monitoring devices due to the limited technology available in the market.

Impact of column temperature on triacylglycerol regioisomers separation in silver ion liquid chromatography using heptane-based mobile phases

This work presents the investigation of the use of heptane as an alternative and less toxic mobile phase to the most used hexane for triacylglycerols (TAGs) analysis in silver ion high-performance liquid chromatography (Ag+-HPLC). The impact of column temperature (in the 5 °C-35 °C range) on the retention and resolution of five pairs of regioisomers relevant for the confectionery industry was investigated using a heptane-based mobile phase modified with acetonitrile (ACN). The retention behaviour was compared for a standard TAG mixture and an interesterified cocoa butter. The temperature effect previously observed with hexane-based mobile phases was confirmed for this new system, and it was also observed that the ACN concentration had an important impact on the strength of the temperature effect, with a higher ACN concentration leading to a lesser impact of temperature on the TAGs’ elution behaviour. In general, the study allowed to conclude on the equivalence of hexane and heptane for TAGs regioisomers separation in Ag+-HPLC, independently of the used temperature or the ACN concentration. In addition, the applicability of heptane-based mobile phases for the separation of TAGs regioisomers was demonstrated on three other confectionary fat samples, namely palm olein, interesterified palm olein, and interesterified shea olein.

Predicting Animal Welfare Labels from Pork Fat Using Raman Spectroscopy and Chemometrics

The awareness of the origin of meat that people consume is rapidly increasing today and with that increases the demand for fast and accurate methods for its distinction. In this work, we present for the first time the application of Raman spectroscopy using a portable spectrometer for the classification of pork. Breeding conditions were distinguished from spectral differences of adipose tissues. The pork samples were obtained from Dutch vendors, from supermarkets with quality marks of 1 and 3 stars, and from a local butcher shop. In total, 60 fat samples were examined using a fiber-optic-coupled Raman spectrometer. Recorded spectra were preprocessed before being subjected to multivariate statistical analysis. An initial data exploration using Principal Component Analysis (PCA) revealed a separation of adipose tissue samples between the lower supermarket quality grade and the samples from the local butcher. Moreover, predictive modeling using Partial Least Squares Discriminant Analysis (PLS-DA) resulted in 96.67% classification accuracy for all three sources, demonstrating the suitability of the presented method for intraspecies meat classification and the potential on-site use.

Ligilactobacillus salivarius MP100 as an Alternative to Metaphylactic Antimicrobials in Swine: The Impact on Production Parameters and Meat Composition.

The metaphylactic use of antimicrobials in swine farms contributes to the emergence of antibiotic-resistant bacteria, which constitutes a major challenge for public health. Alternative strategies are required to eradicate their routine use. In a previous study, metaphylactic antimicrobials were replaced by the administration of Ligilactobacillus salivarius MP100 to sows and piglets for two years. This practice positively modified the fecal microbiota and metabolic profiles in the farm. In this work, the farm dataset was used to compare the productivity-related parameters between a 2-year period of routine metaphylactic antibiotherapy and the first 2 years of a replacement with the probiotic strain. The probiotic period improved these productivity-related parameters, from litter size to growth performance. In addition, samples of Longissimus lumborum, including skin and subcutaneous fat, were obtained from the animals ingesting the probiotic strain and controls (metaphylactic antibiotherapy) and analyzed for their pH, water holding capacity, composition, and metabolic profiling. The probiotic intake did not negatively affect the meat composition and was associated with an increase in inosine concentration and a slight tendency for increasing the intramuscular fat content. These factors are considered as biomarkers of meat quality. In conclusion, the substitution of metaphylactic antimicrobials with the administration of the probiotic strain was associated with beneficial productivity and meat quality outcomes.

Impatto degli antiossidanti sulla qualità e la stabilità ossidativa dei grassi animali

The aim of the work was to determine the influence of natural and synthetic antioxidants on the quality and stability of animal fats. On the samples, with and without the addition of natural antioxidants (extract of sage, rosemary and ginger) added in the fats at a concentracion of 0.2 % and synthetic antioxidants (PG-propyl galate, BHA-butylhydroxyanisole and BHT-butylhidroxytoluene) added in the fats at a concentracion 0.01 % the values of the peroxide value and the content of free fatty acids were tested. The oxidative stability of fats was determined by the sustainability test at 98 °C during 7 days. Every 24 hours, the peroxide value and free fatty acids were examinedid for all samples of beef, sheep and goat fat. The results of the research show that the applied antioxidants successfully stabilized all animal fats compared to the control sample. Analysis of the composition of fatty acids of base samples (without the addition of natural antioxidants) shows that palmitic, stearic and oleic fatty acids are the most abundant in animal fats. The highest total SFA value was found in sheep fat (66.85 %), and the lowest in goat fat (56.57 %). In beef fat, the total SFA content was 59.26 %. Stearic acid is the most abundant in sheep fat (37.75 %), while beef and goat fat had lower and approximately equal values (27.93 % and 26.91 %). After staeric fat, palmitic fat is the mostabundant in beef fat with 27.78 %, followed by 25.25 % in sheep fat and 24.91 % in goat fat. Of the monounsaturated acids (MUFA), the most abundant in goat fat was oleic acid (38.16 %), while it was somewhat lower in beef (36.61 %), and the lowest in sheep (27.60 %). Total unsaturated fatty acids (UFA) are most abundant in goat fat (43.51 %), followed by beef (40.82 %), while they are significantly less present in sheep's fat (32.95 %). Among polyunsaturated acids (PUFA), the highest content of linoleic acid was found in sheep fat (4.19 %). In beef fat, linoleic acid was 60 % lower compared to sheep fat (0.76 %), and 10% higher compared to goat fat (1.89 %). Based on the content of individual fatty acids and the content of certain groups of fatty acids: SFA, UFA, MUFA, PUFA, the nutritional indices of the tested fats were calculated. It was concluded that the tested samples of beef, sheep, and goat fat without the addition of antioxidants can not be declared as products of high nutritional value. The nutritional value expressed through the PUFA/SFA ratio for beef fat was 0,01, and sheep fat and goat fat 0.06.

Application of NIR spectroscopy coupled with DD-SIMCA class modelling for the authentication of pork meat

Rearing system, breed, geographical origin, and animal welfare are some of the more recent quality features able to drive consumer’s choice in meat and meat products purchase. The same aspects are marketing leverage from the producer’s side. However, there is a need to develop rapid analytical strategies to check the compliance of declared quality with actual quality features. To face with this issue, this work aims at test the feasibility of using the fat portion as a marker of authenticity. To this end, fat samples from extensively and intensively reared pigs have been collected and analysed by NIRS (Near Infra-Red Spectroscopy). Then, after multivariate exploration, the Data Driven variant of Soft Independent Modelling of Class Analogy (DD-SIMCA) was used to develop models for the target class. The type of signal pre-processing and the number of principal components (PCs) used strongly affected the performance of the classification. Excellent results were obtained with SNV pre-treated data and 4 PCs that allowed to reach 100% sensitivity and specificity in calibration and validation.