Fat In Cattle Research Articles

Effect of Herbal Liver-tonic Supplements on Production Performance in HF Dairy Cattle

A study was conducted to observe the influence of herbal liver-tonic supplementation on milk production, liver function and feed intake in HF crossbred dairy cattle at field level. This study was carried out in 24 HF cattle and these were randomly grouped into three groups viz. Group 1 served as control group, Group II served as Treatment -I (supplemented with polyherbal liver tonic @ 30ml/day) and Group III as Treatment-2 (supplemented with polyherbal liver tonic @ 45ml/day). Day 1 to 7 is considered as before treatment, Day 8 to 17 as treatment period and day 18 to 32 as after treatment. The blood collected during study period and stored at -20 °C until further analysis. The data were also regularly recorded for milk production, SNF Content of milk, feed intake. Statistical analysis of result revealed that herbal liver-tonic supplementation showed non-significant (P>0.05) difference on liver enzymes profile. Statistically no significant difference is also noted on milk production, milk fat and SNF Content in HF Cattle at field conditions.

Patterns of variation and relationships among fat, protein, and milk yield of individual dairy cattle in a Thai multibreed population.

This study systematically examines the patterns of milk yield (MY, kg), fat (FAT, %), and protein (PROT, %) in a diverse population of Thai multibreed dairy cattle, considering the tropical environment's impact on lactating cows. Using a dataset of 47,205 monthly test-day records from 4,440 first-lactation cows across 446 farms, we analyze variations and interrelationships through mathematical averaging and introduce the fat-to-protein ratio (FPR) to assess acidosis (FPR < 1.1) and ketosis (FPR > 1.5) risks during lactation. Pearson correlation analysis elucidated trait associations. The findings, aligned with established lactation norms, indicate peak production at 297 days in milk (DIM) for FAT (4.08%; SD = 0.96%), PROT (3.43%; SD = 0.47%), and 52 DIM for MY (18.09kg; SD = 4.91kg). Nadirs are observed at 72 DIM for FAT (3.27%; SD = 0.74%), 47 DIM for PROT (2.86%; SD = 0.36%), and 299 DIM for MY (9.05kg; SD = 2.95kg). FPR variations highlight acidosis (46.48%), normal (43.66%), and ketosis (9.86%), especially during early lactation (100 DIM). Significant negative correlations emerge between MY, FAT, and PROT (P < 0.05), while a positive correlation is identified between FAT and PROT (P < 0.01), with robust correlations during early lactation. This study contributes to understanding tailored nutritional strategies for dairy cows' holistic health and sustainability in tropical environments, guiding efficient production practices and mitigating health-related productivity impediments.

A comprehensive analysis of the effects of DGAT1 K232A polymorphism on milk production and fertility traits in Holstein Friesian and Jersey cows reared in Türkiye

Abstract. Research on the diacylglycerol acyltransferase 1 (DGAT1) K232A marker in cattle shows inconsistent results across regions, largely due to small sample sizes, limited genetic variation, and data restricted to few lactations, which complicates establishing a reliable genotype–phenotype correlation. This research aimed to determine the effect of the K232A polymorphism of the bovine DGAT1 gene on milk production and quality traits in dairy cattle. We used 1104 cattle, including 828 Holstein Friesian and 276 Jersey cows. The analysis utilized extensive data from six lactations of cows raised on four commercial dairy farms. We genotyped the population using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique and Sanger sequencing for verification. We then evaluated the 305 d and test-day milk yields as well as fat and protein yields and percentages. The number of inseminations per conception and calving ease were also assessed as reproduction indices. Genotype–phenotype associations were quantified using linear mixed models. The AA genotype was absent in Jersey cows, and the heterozygous genotype was predominant in both breeds. The K232A marker was significantly associated with test-day milk yield, fat, and protein content in Jersey cows. Further, it substantially affected the fat percentage of milk in Holstein Friesian cows (p&lt;0.001). We found that the KK genotype is highly desirable for milk quality and especially fat content. This comprehensive assessment demonstrated that the KK genotype of the DGAT1 K232A polymorphism significantly influenced fat and protein contents in dairy cattle.

Development of a Method for the Determination of Rifaximin and Rifampicin Residues in Foods of Animal Origin.

Rifaximin and rifampicin are good broad-spectrum antimicrobials. The irrational use of antimicrobial drugs in veterinary clinics could threaten public health and food safety. It is necessary to develop a reliable detection method of the residue for enhancing the rational supervision of the use of such drugs, reducing and slowing down the generation of bacterial resistance, and promoting animal food safety and human health. So, this study developed an LC-MS/MS method for the detection of rifaximin and rifampicin residues in animal-origin foods. The residual rifaximin and rifampicin of homogenized test materials were extracted with acetonitrile-dichloromethane solution or acetonitrile in the presence of anhydrous sodium sulfate and vitamin C, purified by dispersible solid phase extraction, determined by LC-MS/MS, and quantified by the internal standard method. The specificity, sensitivity, matrix effect, accuracy, and precision of the method were investigated in the edible tissues of cattle, swine, or chicken. In addition, the stability of the standard stock solution and the standard working solution was also investigated. The method was suitable for the muscle, liver, kidney, fat, milk, and eggs of cattle, swine, or chicken, as well as fish and shrimp. The specificity of the method was good, and the detection of the analytes was not affected by different matrices. Both the LOD and LOQ of the two analytes were 5 μg/kg and 10 μg/kg, respectively. The results of matrix effects in each tissue were in the range of 80-120%; there were no significant matrix effects. The average accuracy of rifaximin and rifampicin in different foodstuffs of animal origin was between 80% and 120%, and the method precision was below 20% (RSD). The proposed method showed good performance for determination, which could be employed for the extraction, purification, and detection of residual rifaximin and rifampicin in edible animal tissues. The pretreatment procedure of tissue samples was simple and feasible. The method was highly specific, stable, reliable, and with high sensitivity, accuracy, and precision, which met the requirements of quantitative detection of veterinary drug residues.

Isolation of Cutibacterium acnes AP1, a rumen bacterium that forms t10,c12-conjugated linoleic acid

Some microbes in the rumen form t10,c12-conjugated linoleic acid (CLA), a fatty acid that depresses synthesis of milk fat in dairy cattle and other lactating animals. Despite their importance to milk fat depression, the microbes responsible have been difficult to identify, and no laboratory strain is currently available for study. Here we describe the isolation of Cutibacterium acnes AP1, a bacterium that forms t10,c12-CLA at fast rates. It was isolated from rumen contents of a heifer by using media containing lactate and metronidazole. It formed t10,c12-CLA from linoleic acid, a result confirmed by a combination of 3 methods (spectrophotometry, thin-layer chromatography, gas chromatography). The initial rate of formation was 67% faster than for Cutibacterium acnes DSM 1897, the type strain from human skin. The availability of this isolate and its ability to rapidly form t10,c12-CLA make it a good subject for further study. Such study could help control formation of t10,c12-CLA in the rumen and alleviate milk fat depression in milk cows.

CircSSBP2 acts as a MiR-2400 sponge to promote intramuscular preadipocyte proliferation by regulating NDRG1.

Intramuscular fat (IMF) is a critical factor in beef quality. IMF is mainly distributed between muscle fibres and its accumulation can affect the marbling and meat quality of beef. IMF formation and deposition is a complex process and in recent years a group of non-coding RNAs (ncRNAs), known as circRNAs, have been discovered to play an important role in regulating intramuscular fat deposition. CircRNAs form a covalent loop structure after reverse splicing of precursor mRNAs. They can act by adsorbing miRNAs, thereby reducing their repressive effects on downstream target genes. Based on high-throughput sequencing of circRNAs in intramuscular fat of Qinchuan and Japanese black cattle, we identified a novel circSSBP2 that is differentially expressed between the two species and associated with adipogenesis. We show that circSSBP2 knockdown promotes bovine intramuscular preadipocyte proliferation, whereas overexpression inhibits bovine intramuscular preadipocyte proliferation. We also show that circSSBP2 can act as a molecular sponge for miR-2400 and that miR-2400 overexpression promotes bovine intramuscular preadipocyte proliferation. Furthermore, N-myc downstream-regulated gene 1 (NDRG1) was identified as a direct target gene of miR-2400, and NDRG1 interference promoted the proliferation of bovine intramuscular preadipocytes. In conclusion, our results suggest that circSSBP2 inhibits the proliferation of bovine intramuscular preadipocytes by regulating the miR-2400/NDRG1 axis.

The genetic polymorphism of c.-1221A>C and discovery of novel SNPs in the promoter region of Mitochondrial Transcription Factor A (TFAM) gene in Bali Cattle

The expression of the mitochondrial transcription factor A (TFAM) gene has a marked impact on mitochondrial biogenesis. It is suggested to play a critical role in lipogenesis, including the deposition of marbling fat in cattle. Bali cattle (Bos javanicus) are known for their lean meat but exhibit poor marbling scores. This study aimed to investigate the polymorphism of c.-1221A>C and novel SNPs in the promoter region of the TFAM gene in Bali cattle. A total of 245 Bali cattle individuals were included in this study. Three Bali, three Wagyu, and one Limousin cattle were used for sequencing analysis. Genomic DNA was extracted either from blood or sperm for genotyping and variant analysis. The c.-1221A>C SNP was genotyped by PCR-RFLP, and the novel SNPs of the TFAM gene promoter region were identified by sequencing analysis. Our results revealed that the c.-1221A>C SNP was found to be monomorphic, with the presence of a fixed A allele, making it unsuitable for further association study in Bali cattle. However, the study successfully identified four novel SNPs and one novel DNP found in the promoter region of the TFAM gene. Notably, one double nucleotide polymorphism (DNP) of g.[45690945C>T;45690946A>G] was unique in Bali cattle compared to other breeds. In addition, only the c.-911T>A SNP was found heterozygous within the Bali cattle samples. These SNPs provide valuable insights into the genetic diversity of the TFAM gene in Bali cattle and serve as a basis for future investigations on the distinctiveness of Bali cattle, especially in the efforts of enhancing the quality of Bali beef.

Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming.

This study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP-not programmed or control, without protein-energy supplementation; PP-partially programmed, with protein-energy supplementation (0.3% of body weight (BW)) only in the final third of pregnancy; and FP-full programming, with protein-energy supplementation (0.3% of BW) during the entire pregnancy. The meat and fat samples were collected individually 24 h after slaughter, and the metabolites were extracted using a combination of chemical reagents and mechanical processes and subsequently quantified using liquid chromatography or flow injection coupled to mass spectrometry. The data obtained were submitted to principal component analysis (PCA), analysis of variance (ANOVA), and functional enrichment analysis, with a significance level of 5%. The PCA showed an overlap between the treatments for both meat and fat. In meat, 25 metabolites were statistically different between treatments (p ≤ 0.05), belonging to four classes (glycerophospholipids, amino acids, sphingolipids, and biogenic amine). In fat, 10 significant metabolites (p ≤ 0.05) were obtained in two classes (phosphatidylcholine and lysophosphatidylcholine). The functional enrichment analysis showed alterations in the aminoacyl-tRNA pathway in meat (p = 0.030); however, there was no pathway enriched for fat. Fetal programming influenced the meat and fat metabolomes and the aminoacyl-tRNA metabolic pathway, which is an important candidate for the biological process linked to meat quality and related to fetal programming in beef cattle.

PSIII-15 Use of Ultrasound Technology to Estimate Final Carcass Composition in Harvested Beefmaster Steers

Abstract The objective of this research study was to determine the accuracy of ultrasound scanning to predict post-harvest carcass data. Beefmaster steers (n = 47) representing two different sires were ultrasounded 62 d before harvest using an Aloka 500 ultrasound unit, equipped with a 17.2cm probe to collect 12th rib fat thickness (FTU), 12th rib longissimus muscle area (LMAU), intramuscular fat (%FatU) and rumpfat (RFU). Beef Image Analysis (BIA) software was used to determine FTU, REAU, %FatU and RFU using chuteside captured ultrasound images on all steers. Carcass data collection included 12th rib fat thickness (FTC), 12th rib longissimus muscle area (REAC) and marbling score (MS). Additionally, Warner-Bratzler shear force (WBSF) and ether extraction (EE) estimates were determined on 12 to 13th longissimus muscle samples. All carcass data were collected by the Beef Carcass Research Center at West Texas A&amp;M University. Data were analyzed using SAS version 9.4. Simple descriptive statistics were 1.03 ± 0.25 cm, 90.1 ± 8.64 cm2, 457.3 ± 74.7, 1.35 ± 0.38, 5.75 ± 1.83, 3.86 ± .64 kg for FTU, REAU, %FatU, RFU, EE and WBSF, respectively. Simple descriptive statistics were 18 ± 0.35 cm, 89.72 ± 7.74 cm2, and 504.7 ± 67.2 for FTC, REAC and MS, respectively. Pearson correlation coefficients were 0.72 (P &amp;lt; 0.0001) between FTU and FTC, 0.52 (P &amp;lt; 0.0002) between REAU and REAC, and 0.45 (P &amp;lt; 0.0017) between %FatU and MS. Pearson correlation coefficients were 0.45 (P &amp;lt; 0.0017) between EE and %FatU and 0.72 (P &amp;lt; 0.0001) between EE and MS. These results suggest that ultrasound measures of final body composition should be taken closer to the final harvest date to better estimate endpoints of fat thickness, longissimus muscle area and intramuscular fat in harvest ready beef cattle.

Isotopic internal standard correction for the determination of diludine residue in animal-derived matrix samples using enhanced matrix removal-lipid clean-up combined with ultra-performance liquid chromatography-tandem mass spectrometry

A robust method has been established for the successfully determination of diludine (DHP) levels in animal-derived matrix samples, utilizing enhanced matrix removal-lipid clean-up (EMR-lipid) combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). DHP-13C4 was employed as an isotopically labeled internal standard to estimate the recovery, matrix effect, and process efficiency. The water-acetonitrile mixture was utilized to extract the edible tissues (muscle, fat, kidney, and liver) of cattle and chicken, while the salting out method was employed for extracting egg and milk. The extracts were then mixed with water and acetonitrile to a constant volume, and subjected to clean-up via cartridge. Subsequently, the analytes were transferred onto a reversed-phase C18 analytical column and quantified by triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode. Quantification was accomplished using a solvent calibration curve in conjunction with an internal standard method. The proposed method showed good linearity in the range of 0.5–50 ng mL−1 (R2 ≥ 0.999), high sensitivity (the limit of detection was 2.5 μg kg−1, and the limit of quantification was 5 μg kg−1), a high spiked recovery rate (between 82.6% and 109.4%), and repeatability (the relative deviation within the batch and the relative deviation between batches were ≤ 7.5%).

Preliminary Estimation of Nutritional Quality of the Meat, Liver, and Fat of the Indigenous Yakutian Cattle Based on Their Fatty Acid Profiles.

The Yakutian cattle is an indigenous Siberian cattle breed living in an extremely cold climate in some parts of Yakutia. There are only a few thousand animals of this breed, and the conservation of the Yakutian cattle is embedded in the international agenda. We studied the fatty acid profiles in the meat, liver, and fat of the Yakutian cattle (five individuals) of different ages and their main food resource-pasture plants. The fatty acid profile of the tissues of the Yakutian cattle differed from that of pasture plants: 16:0, 18:2n-6, and 18:3n-3 dominated in the pasture plants; 16:0, 18:0, 18:1n-9, 18:2n-6, 20:4n-6, 20:5n-3, and 22:5n-3 dominated in the meat and liver; and 16:0, 18:0, and 18:1n-9 dominated in the fat. The fatty acid composition of food products is related to the risk of developing cardiovascular disease (CVD). The meat and liver of the Yakutian cattle are health food products that contribute to decreasing the risk of developing CVD because of their rather high content of eicosapentaenoic and docosahexaenoic fatty acids, optimal n-6/n-3 and polyunsaturated fatty acids/saturated fatty acids ratios, low values of indexes of atherogenicity and thrombogenicity, and high values of hypocholesterolemic/hypercholesterolemic and health-promoting indexes. The results of the present study support the importance of preserving this valuable cattle breed. Actions should be taken to increase their population while retaining their contemporary housing and feeding conditions.

Effect of age and sexual condition on the fatty acid profile of intramuscular fat of cattle finished in feedlot

The objective of this study was to evaluate the fatty acid (FA) profile of intramuscular fat of cattle of two categories (16 or 26 months old) and two sexual conditions (Bulls or Steers) finished in feedlot. A total of 32 castrated (n=16) or noncastrated (n=16) animals of the genetic groups ½Purunã + ½Canchim were used. The animals were finished in feedlot, receiving a diet with 50% roughage and 50% concentrated feed. After the slaughter, one Longissimus dorsi sample from each animal was sent to the laboratory for FA profile analyses. The FA content of C17:0 iso and C18:0 was higher for animals slaughtered at 26 months. Lignoceric acid (C24:0) showed a higher content for animals aged 16 months. Animals slaughtered at 16 months showed higher C18:1 cis 11, C18:1 cis 12, C18:1 cis 15, and lower C18:1 trans 16 than those slaughtered at 26 months. The C20:4 and C22:5 were lower as the animals' slaughter age increased. The unsaturated/saturated and polyunsaturated/saturated ratios showed no difference for slaughter age. The concentrations of C24:0, C18:1 trans 10, C18:1 cis 11, C18:1 cis 12, and C22:1 were greater in the meat of bulls than in steers. Bulls had a higher content of polyunsaturated FAs and polyunsaturated/saturated ratio than steers.

EFFECT OF MASTITIS ON MILK COMPOSITION OF CROSS-BRED (HOLSTEIN FRIESIAN X CHOLISTANI) AND SAHIWAL CATTLE

The present study was designed to investigate the effect of mastitis on various components including fat percentage, protein, total solids, solid not fat, pH, specific gravity and lactose content in milk of cross-bred (Holstein Friesian × Cholistani) and Sahiwal cattle. A total of 376 milk samples (excluding blind and nonfunctional teats) from 100 cows (50 cross-bred and 50 sahiwal) were collected aseptically. Animals of different ages, parity and stages of lactation were selected under field condition. The diagnosis of subclinical mastitis was done on basis of Surf Field Mastitis Test. For bacterial examination of milk, the samples were grown in laboratory and various biochemical tests were performed. The results of the present study indicated that solid not fat (SNF) of mastitic sahiwal and cross-bred cattle and total solids percentage of normal and mastitic cattle were significantly reduced as the severity of mastitis increased in animals. The milk pH of normal and mastitic cattle including both sahiwal and cross-bred was significantly increased in severe cases of mastitis. The specific gravity of normal and mastitic milk samples showed that there was no significant difference in specific gravity as compared to normal. The protein percentage of normal and mastitic cattle including both sahiwal and cross-bred cattle indicated that protein concentration was significantly reduced in severe cases of mastitic animals. The findings of present study highlighted that mastitis cause major alterations in composition of milk leading to poor quality of the milk.

Effect of Vitamin A, C, and D on improving intramuscular fat in Hanwoo beef cattle - Review

Effect of Vitamin A, C, and D on improving intramuscular fat in Hanwoo beef cattle - Review

Cold exposure induces browning of bovine subcutaneous white fat in vivo and in vitro

White adipocytes can be transformed into beige adipocytes through the process of browning under cold exposure. To investigate the effects and underlying mechanisms of cold exposure on subcutaneous white fat in cattle, in vitro and in vivo studies were performed. Eight bulls of Jinjiang cattle breed (Bos taurus) aged 18 months were allocated to the control group (n = 4, autumn) or the cold group (n = 4, winter) by different slaughter seasons. Biochemical and histomorphological parameters were detected in blood and backfat samples. Subcutaneous adipocytes from Simental cattle (Bos taurus) were then isolated and cultured at a normal body temperature (37 °C) and at a cold temperature (31 °C) in vitro. In the in vivo study, cold exposure stimulated subcutaneous white adipose tissue (sWAT) browning by reducing adipocyte sizes and up-regulating the expression levels of browning-specific makers (UCP1, PRDM16, and PGC-1α) in cattle. In addition, cold-exposed cattle displayed lower lipogenesis transcriptional regulator levels (PPARγ and CEBPα) and higher lipolysis regulator levels (HSL) in sWAT. In the in vitro study, cold temperature inhibited subcutaneous white adipocytes (sWA) adipogenic differentiation by reducing lipid contents and decreasing the expression of adipogenic marker genes and proteins. Furthermore, cold temperature led to sWA browning which was characterized by increased browning-related genes, mitochondrial contents, and mitochondrial biogenesis-specific markers. In addition, p38 MAPK signaling pathway activity was stimulated by the incubation in cold temperature for 6 h in sWA. We concluded that the cold-induced browning of the subcutaneous white fat was beneficial to the production of heat and the maintenance of body temperature regulation in cattle.

Determination of Chlorothalonil Metabolite I in Livestock Products by LC-MS/MS

An analytical method based on LC-MS/MS was developed for the determination of chlorothalonil metabolite I in livestock products. Chlorothalonil metabolite I in livestock products was extracted with acetone. The crude extracts were defatted by acetonitrile and n-hexane partitioning. Cleanup was carried out using a combination of ethylene diamine-N-propyl silylation silica gel (PSA) and silica gel (SI) mini columns with acidic condition. The sample solution was subjected to LC-MS/MS using an external solvent calibration curve. The average recovery (n=5) of chlorothalonil metabolite I from five types of livestock products (cattle muscle, cattle fat, cattle liver, milk and egg) spike at the maximum residue limits (MRLs) or at a uniform limit of 0.01 mg/kg was 97.1-102.9%, with a relative standard deviation of 1.4-6.8%. The limit of quantitation of the developed method was calculated to be 0.01 mg/kg.

Cis-regulatory modules prediction in spliced genes associated with carcass and meat traits of Nelore cattle

The cis-regulatory modules are non-coding DNA regions responsible for controlling gene transcription and being involved in the anatomical and embryonic development of animal species. This study aimed to perform a prediction analysis of cis-regulatory modules in spliced genes associated, commonly, with ribeye area (REA) and intramuscular fat (IF) of Nelore cattle. For this, JuncBASE v.0.9 (Junction-Based Analysis of Splicing Events) software was used to identify and classify exon-centered alternative splicing events in the group of animals selected for REA and IF. The prediction of transcription factors and cis-regulatory modules was performed for the genes found using iRegulon v.1.3. The prediction analysis exhibited 45 cis-regulatory modules, of which module 11 had the highest number (N = 9) of binding sites shared commonly by 11 spliced genes and five transcription factors from the MEF2 family. These modules could potentially regulate spliced exons and, thus, contribute to the production of isoforms, which may be involved in essential pathways and biological processes involved in the evaluated animals muscular development and lipid metabolism.

Bta-miR-493 Inhibits Bovine Preadipocytes Differentiation by Targeting BMPR1A via the TGFβ/BMP and p38MAPK Signaling Pathways.

Fat deposition significantly impacts the meat yield and the meat quality of beef cattle, which is closely associated with the preadipocyte proliferation and differentiation. Bta-miR-493 is expressed differentially in the backfat of Qinchuan cattle of various ages, and it may be involved in the fat accumulation of beef cattle. However, the role and functional mechanism of bta-miR-493 in fat deposition are still unclear. Tissue-specific expression analysis showed that the level of bta-miR-493 was moderately abundant in the adipose tissues of beef cattle. Moreover, the expression of bta-miR-493 in perirenal fat, subcutaneous fat, and longissimus dorsi muscle of Japanese black cattle was significantly higher than that in Qinchuan cattle. EdU staining, cell counting assay, and Oil Red O staining indicated that bta-miR-493 promoted the proliferation of bovine preadipocytes but inhibited their differentiation. The dual luciferase assay and transcriptomic analysis confirmed that bone morphogenetic protein receptor 1A (BMPR1A) is a target gene of bta-miR-493. Moreover, rescue experiments showed that bta-miR-493 could promote bovine preadipocyte proliferation but inhibit their differentiation by targeting BMPR1A via the TGFβ/BMP and p38MAPK signaling pathways. Overall, our findings revealed a bta-miR-493-BMPR1A-TGFβ/BMP/p38MAPK regulatory axis that will serve as a theoretical foundation for the molecular breeding of beef cattle with high intramuscular fat deposition.

ИНТЕНСИФИКАЦИЯ ПРОЦЕССА ИЗВЛЕЧЕНИЯ ЖИРА ИЗ ЖИРОСОДЕРЖАЩЕГО СЫРЬЯ ДИЭЛЕКТРИЧЕСКИМ НАГРЕВОМ

The article substantiates the effective modes of heat treatment of fat-containing raw materials in a radiohermatic ultrahigh-frequency installation with a metal-dielectric resonator.&#x0D; The raw material for the study was the internal fat of cattle, sheep, chickens, their dielectric characteristics were systematized according to I.A. Rogov.&#x0D; The purpose of this work is to develop a continuous-flow microwave installation with air-cooled magnetrons and a resonator in the form of a truncated cone with an exponential generator, which contributes to increasing thermal efficiency and maintaining high electric field strength and radio tightness without a shielding housing when heating disinfected fat from fat-containing raw materials. The electrophysical parameters of the fat-containing raw material are analyzed, including the change in dielectric parameters from the frequency of the electromagnetic field (EMF), fat content, density. The intensity of the electric field is matched with the heating rate, the specific power of the generator. A continuous-flow microwave installation with a resonator in the form of a truncated cone with an exponential generator made of non-ferromagnetic material and a ceramic concave mirror base, inside of which a dielectric screw with a decreasing pitch and a diameter of a screw is located, has been developed. A concave ceramic mirror is installed coaxially on the large base of the resonator. A dielectric screw is inscribed along the resonator. Two sluice gates with non-magnetic bent blades are installed on the resonator. The melted fat flows down through the perforation on the surface of the resonator. Magnetrons with waveguides are installed with a shift of 120 degrees along the perimeter of the resonator base. The general properties of a resonator with dielectric inclusions (ceramic mirror, fluoro-formation auger) are analyzed. In such a metal-dielectric resonator, the reflection of the field from the non-magnetic walls of the resonator and critical sections can be combined with full internal reflection in the dielectric.&#x0D; The exponential generator of the resonator and the ceramic mirror contribute to an increase in the concentration of the electric field strength in it to 2 kV/cm when placing three magnetrons with a shift of 120 degrees along the perimeter of the large base. With a specific power of the generator of 2 W/g, the productivity of the microwave installation for heat treatment of fat-containing raw materials is 18-20 kg/h.; With a frequency of rotation of the electric screw drive of 1 rpm, 0.5-0.6 kg of raw materials is heated in 120 seconds. The specific energy costs compared to the prototype (0.2-0.25 kWh/kg) is reduced to 0.14-0.15 kWh/kg.

Low lysine stimulates adipogenesis through ZFP423 upregulation in bovine stromal vascular cells

Adipogenesis is a complex process comprising commitment and a differentiation stages. Through research, many different transcriptional factors were found to mediate preadipocyte commitment and differentiation. Lysine has a potential of regulating the commitment and differentiation of preadipocytes. In the present study, intramuscular stromal vascular cells (SVC) isolated from Hanwoo beef cattle were used to elucidate the effects of low lysine level on adipogenesis. SVC were isolated and incubated with various concentrations of lysine (0, 37.5, 75, 150 and 300 µg/mL). No significant difference were observed in the proliferation of SVC after 24 and 48 h of incubation with different concentration of lysine. On preadipocyte determination, reducing the level of lysine significantly increased the expression of preadipocyte commitment gene Zinc finger protein 423 and Preadipocyte factor-1. Upon differentiation, Oil Red O staining revealed that lipid accumulation and triglyceride content significantly increased with the decreasing lysine levels in the media. Expression levels of peroxisome proliferator-activated receptor-γ, CCAAT enhancer binding protein-α, sterol regulatory element binding protein-1c, Fatty Acid Binding Protein 4 and stearoyl CoA desaturase were upregulated by the decreased level of lysine. These data suggest the potential mechanism of action for the improved preadipocyte commitment and adipocyte differentiation in bovine intramuscular SVC upon treatment with low levels of lysine. These findings may be valuable in developing feed rations that promote deposition of intramuscular fat in beef cattle through lysine level modification.