Fat Grafting For Facial Rejuvenation Research Articles

Anti-photoaging Effect of Micronized Fat in Ultraviolet B-Induced Human Dermal Fibroblasts.

Adipose-derived stromal vascular fraction and mesenchymal stem cells have been proven to reduce the effects of skin photoaging. However, there is no standardized protocol for their preparation. This study aimed to investigate the skin rejuvenation potential of micronized fat, obtained using a novel device attached with a trifoliate blade, in the ultraviolet B-induced human dermal fibroblasts model. Micronized fat was prepared to obtain adipose-derived stromal vascular fraction and the adipose-derived mesenchymal stem cells to stromal vascular fraction ratio was determined by flow cytometry. The ultraviolet B-induced human dermal fibroblasts model was constructed to identify the characteristic of the human dermal fibroblasts using Vamiten and S-100 immunostaining, observe their morphology, and measure the levels of photoaging related factors. After the previous steps were completed, different cell groups were co-cultured with ultraviolet B-induced human dermal fibroblasts and the extent of improvement of photoaging was evaluated. Micronized fat had a higher adipose-derived mesenchymal stem cells to stromal vascular fraction ratio than the control fat preparations. The ultraviolet B-induced human dermal fibroblasts model showed lowered levels of type I collagen and transforming growth factor-βand increased expression of matrix metalloproteinases, which are the characteristics of photoaging in normal human dermal fibroblasts. Compared to different cell groups co-cultured with ultraviolet B-induced human dermal fibroblasts, micronized fat could lower the expression of matrix metalloproteinases and increase the level of type I collagen but lower the level of transforming growth factor-β. Obtaining micronized fat is more effortless and clinically safer. Micronized fat has an anti-photoaging effect by inhibiting the expression of MMPs via MAPK signaling pathway.

Sequential Grafting of Fresh and Cryopreserved Fat After Mechanical Processing is a Safe and Effective Facial Rejuvenation Strategy.

The survival rate of fat transplants is variable and consequently multiple operations are often required to achieve satisfactory results. Fat cryopreservation technology is a good solution to this problem. At present, cryopreservation of fats needs to be added with cryopreservation agents, which brings unsafety and operational complexity to clinical applications. An efficient and safe strategy for fat cryopreservation must be developed. A retrospective study was performed of all patients who underwent facial fat grafting and agreed to have their fat tissue cryopreserved from January 2018 to May 2021. Fat samples were physically processed to obtain SVF-gel, which was cryopreserved at - 20°C for up to 3 months and injected after thawing. Images acquired by pre- and post-operative 3D scanning of the temporal region were compared to evaluate the retention rate of transplanted cryopreserved SVF-gel. No patients experienced serious complications after receiving cryopreserved fat transplantation. The retention rate of cryopreserved SVF-gel was 46.3 ± 7.7% at 3 months and 43.1 ± 7.2% at 6 months after transplantation. The swelling duration was significantly shorter after cryopreserved SVF-gel transplantation (5.5 ± 0.8 days) than after fresh fat transplantation (7.5 ± 0.7 days) (p < .031). Injection of cryopreserved SVF-gel achieves good retention rate for facial rejuvenation and has few side effects. Cryopreservation of SVF-gel is a safe and effective strategy for serial fat grafting for facial rejuvenation. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Fat Grafting for Facial Rejuvenation and Contouring: A Rationalized Approach.

The clinical outcome after fat grafting for facial rejuvenation and contouring can be less optimal because lack of standardized techniques is performed by the surgeon for the procedure. In this review, the author has tried to provide a rationalized approach to fat grafting for facial rejuvenation and contouring. The preferred donor sites include low abdomen and inner thigh especially in younger patients. Fat grafts should be harvested with low negative pressure via syringe liposuction technique to ensure the integrity as well as the optimal level of cellular function. Fat grafts should be processed with proper centrifugation that can reliably produce purified fat and concentrated growth factors and adipose-derived stem cells, all of which are beneficial to improve graft survival. Fat grafts should be placed after certain principles with gentle injection of small amount during the withdraw phase of each pass in multiple tissue planes and levels with multiple passes to ensure maximal contact of graft with vascularized tissue in the recipient site. The approach described in this review is supported by the most recent scientific studies and thus can likely provide a more predictable long-lasting result if performed properly.

Autologous Fat Grafting to the Chin: A Useful Adjunct in Complete Aesthetic Facial Rejuvenation.

The chin makes up the central unit of the face and neck, and is a significant factor in facial harmony and aesthetics. Historically, correction of microgenia required surgical intervention with a sliding genioplasty, or placement of a permanent implant. However, these techniques require more extensive surgical intervention, prolonged downtime, with higher costs and complications. Furthermore, chin rhytides and descent of chin fat lead to an aged appearance of the chin and lower face that is difficult to correct with a chin implant alone. Autologous fat grafting in facial rejuvenation has expanded in its application and can serve as an effective technique to correct and enhance chin aesthetics, including lateral chin hollowing, asymmetry, mild microgenia, and correction of deep labiomental sulcus or a bifid chin. This article and accompanying video demonstrate the authors' technique for chin augmentation and refinement with autologous fat.

The Downside of Fat: Avoiding and Treating Complications.

Three-dimensional facial volume restoration using fat has become widely accepted as an essential component of facial rejuvenation. Transplanted fat has benefits due to its inherently nonallergenic nature. The versatility of fat grafts allows for their use in all types of facial enhancement-improving the appearance of nasolabial folds, mesolabial grooves, flattened cheeks and upper lips, glabellar furrows, lipoatrophy, acne scars, and temporal hollowing. Yet despite its virtues as the ideal filler, autologous fat has its shortcomings with risks of complications. Our objective in this article is to present a systematic approach demonstrating the complications that can occur with each step of autologous fat grafting in facial rejuvenation and offering pearls to avoid and treat these complications.

Canadian Society of Plastic Surgeons

Atrophy plays an extremely important role in facial aging. The current model of aging is based upon loss of volume in the face as the primary physical sign of aging followed or accompanied by sagging or descent of soft tissues. Restoration of fullness to the atrophic, aging upper and mid-face is essential for successful rejuvenation. Adding fullness to the face is a natural, logical approach to rejuvenation. In the early 1990’s, I first noted that transplanted fat did more than just create fullness. The implanted fat improved the quality of the tissues into which it was grafted. This initial improvement is then usually followed by a continued, gradual long-term improvement in the quality of the skin. Wrinkles soften, pore size decreases and pigmentation improves in patients followed over ten years. In addition, fat grafted under depressed scars not only relieves the depression, but also softens or even completely eliminates the specific scar tissue, making it look like normal skin. More recently we have noted the effect of fat grafts on therapeutic radiation injuries. But grafted fat does more than simply restore fullness. Changes occur in skin overlying the grafted sites over time (for instance, immediately after implantation, in the first months, at one year, three years, five years, eight years etc). The volume of the fat appears to stabilize four months after the procedure, but the texture of sun damaged or scarred skin continues to improve dramatically in the ensuing months. The improvement usually continues up to five years and in many cases for almost a decade. While fat grafting was used mainly in aesthetic surgery for many years, we are now applying these same techniques for reconstructive surgery to restore or create facial or corporal forms by manipulation of soft tissues. Examples of facial and body reconstruction will be presented. Structural fat grafting can be a relatively predictable and safe procedure with a low rate of complications and a high degree of patient satisfaction. Further studies are essential to understand more clearly the mechanism of grafted fat tissue and increase predictability. Learning Objectives: To better appreciate the potential of structural fat grafting in facial rejuvenation. To identify the potential effect of fat grafts on scarring. To understand the potential uses in facial and corporal reconstruction. To introduce recent advances in the use of fat grafting. Disclosure of Interest: Dr. Coleman has noted the following: I have the potential for stock options with Cytori Therapeutics and Brava. I do research into techniques using products from Mentor. I receive royalties and consulting fees from Mentor. Can J Plast Surg. 2010 Summer; 18(2): 41–76. » 01 An Audit of Primary Palatoplasty; Results from One Surgeon and 485 Cases Over 10 Years Can J Plast Surg. 2010 Summer; 18(2): 41. 01 An Audit of Primary Palatoplasty; Results from One Surgeon and 485 Cases Over 10 Years Mh Mahoney and D Fisher Copyright and License information ► Copyright © 2010, Pulsus Group Inc. All rights reserved Abstract PURPOSE: The purpose of this study is the review of a single surgeon’s protocol and experience in primary palatoplasty over a 10 year period at a single cleft centre.

Combining chemical peels with fat grafting for facial rejuvenation

Combining Chemical Peels With Fat Grafting for Facial Rejuvenation Get access Tolbert S. Wilkinson, MD Tolbert S. Wilkinson, MD San Antonio, TX Search for other works by this author on: Oxford Academic PubMed Google Scholar Aesthetic Surgery Journal, Volume 15, Issue 4, December 1995, Page 16, https://doi.org/10.1016/S1090-820X(05)80033-0 Published: 01 December 1995