Abstract
Adipose-derived stromal vascular fraction and mesenchymal stem cells have been proven to reduce the effects of skin photoaging. However, there is no standardized protocol for their preparation. This study aimed to investigate the skin rejuvenation potential of micronized fat, obtained using a novel device attached with a trifoliate blade, in the ultraviolet B-induced human dermal fibroblasts model. Micronized fat was prepared to obtain adipose-derived stromal vascular fraction and the adipose-derived mesenchymal stem cells to stromal vascular fraction ratio was determined by flow cytometry. The ultraviolet B-induced human dermal fibroblasts model was constructed to identify the characteristic of the human dermal fibroblasts using Vamiten and S-100 immunostaining, observe their morphology, and measure the levels of photoaging related factors. After the previous steps were completed, different cell groups were co-cultured with ultraviolet B-induced human dermal fibroblasts and the extent of improvement of photoaging was evaluated. Micronized fat had a higher adipose-derived mesenchymal stem cells to stromal vascular fraction ratio than the control fat preparations. The ultraviolet B-induced human dermal fibroblasts model showed lowered levels of type I collagen and transforming growth factor-βand increased expression of matrix metalloproteinases, which are the characteristics of photoaging in normal human dermal fibroblasts. Compared to different cell groups co-cultured with ultraviolet B-induced human dermal fibroblasts, micronized fat could lower the expression of matrix metalloproteinases and increase the level of type I collagen but lower the level of transforming growth factor-β. Obtaining micronized fat is more effortless and clinically safer. Micronized fat has an anti-photoaging effect by inhibiting the expression of MMPs via MAPK signaling pathway.
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