Fat Consumption Research Articles

Performance variables and nutritional status analysis from Moroccan professional and adolescent football players during the competition period: a descriptive study

IntroductionNutrition plays an integral role in optimizing football players’ performance during training sessions and matches and maintaining their overall health throughout the season. This study aimed to evaluate how well the dietary practices of professional and adolescent football players in Morocco during the competitive period met international macronutrient recommendations, and to explore the relationship between their nutritional status and aerobic performance, as measured by the Yo-Yo IRL1 test.MethodsA total of 277 footballers from Morocco's professional league, “Botola-Pro”, were monitored over a seven-day (training microcycle) during the competitive period. The dietary intake was assessed through self-reported methods and 24-hour recalls. Relevant body composition was measured with bioelectrical impedance (BI), and aerobic performance was evaluated using the Yo-Yo IR test.ResultsThe results indicated significant variations in performance and nutritional status across different categories and age groups. The nutritional status of the players didn't match the UEFA recommendations (p < 0.001). We found that higher intake levels of carbohydrates and proteins were positively correlated with the total distance covered by the players (p < 0.001, r = 0.63, R2 = 0.4 for carbohydrates; p < 0.001, r = 0.59, R2 = 0.35 for proteins). Conversely, a higher proportion of energy derived from fats in the diet was negatively correlated with the distance covered (p < 0.001, r = −0.64, R2 = 0.41).ConclusionThese findings suggest that optimizing carbohydrates and protein intake while managing fat consumption is crucial for enhancing sporting performance. This information is essential for tailoring training programs and nutritional regimens based on the competition level.

Association of FTO variants rs9939609 and rs1421085 with elevated sugar and fat consumption in adult obesity

This cross-sectional study explores the impact of FTO gene single nucleotide polymorphisms (SNPs) rs9939609 and rs1421085 on dietary habits contributing to obesity risk in Thai adults. The study enrolled 384 participants from Bangkok, categorized as non-obese (BMI < 25 kg/m2) or obese (BMI ≥ 25 kg/m2) based on WHO Asia Pacific Guidelines. Genotyping for FTO variants was performed using DNA from blood samples. While both SNPs adhered to Hardy–Weinberg equilibrium, the association between risk alleles and anthropometric measurements was not statistically significant. However, risk allele carriers showed significantly higher intakes of sugar and saturated fat compared to homozygous dominant individuals. In the obese group, the odds ratio for high-sugar intake was 2.22 (95% CI 1.13–4.37, p = 0.021) for rs9939609 risk allele carriers. For high-saturated fat intake, the odds ratio was 1.86 (95% CI 1.02–3.40, p = 0.041). Similar associations were observed for rs1421085. Risk allele carriers also exhibited significantly higher leptin levels (p < 0.043) and a positive correlation with myeloperoxidase levels (p < 0.038). These findings highlight the complex relationship between FTO risk alleles, increased consumption of sugar and saturated fat, and obesity-related parameters. The insights emphasize the importance of considering both genetic and dietary factors in obesity prevention strategies.

Red Wine Consumption and the Impact on Cardiovascular Health: The Methodological Barriers of Observational Study Design.

Cardiovascular disease (CVD) is the leading cause of death among communicable and noncommunicable diseases, and its prevalence is going to rise even more by 2030. The discovery of different "functional" foods containing a plethora of bioactive compounds is considered an ally in the effort to reduce the global CVD burden in the context of primary prevention. It has been about 3 decades since the observation that red wine consumption in French population could lead to lower coronary heart disease risk despite the high dietary consumption of saturated fats, known as the "French paradox." Since then, numerous epidemiological studies, mainly observational, have emerged in order to investigate this association with great enthusiasm. However, due to the nature of these studies, the scientific community has raised concerns about the methodological approach of the studies and thus the generalization of their results. Therefore, the current review aims to summarize some of the major methodological issues deriving from observational studies on the association between red wine consumption and cardiovascular health and to highlight the importance of higher quality study design in the general effort of drawing safer conclusions on this topic.

Sleep, dietary intake and physical activity changes with onset of shift work in intern paramedics

Paramedic work is associated with high staff turn-over, insufficient sleep and adverse health outcomes. Our understanding of the relationship between paramedic work and the health behaviours that may precede adverse health outcomes is limited, as few longitudinal studies have examined health behaviours in workers before they commence on the road work. Such research is needed to inform evidence-based recommendations specifically for new paramedics. The current observational cohort study examined self-reported sleep, dietary intake and physical activity in 21 intern paramedics: 15 female and 6 male (median age: 23.0 years [interquartile range (IQR): 4.0]) from an Australian ambulance service. A subset of participants ( n = 16) provided fasting blood samples for cardiometabolic markers (cholesterol, triglycerides and fasting glucose). Data were collected quarterly (pre-shift work, 3 months, 6 months, 9 months and 12 months post commencement). Linear mixed models showed that the first 12 months of shift work were associated with poorer sleep quality ( p = .036), consumption of fewer kilojoules ( p = .026), reduced saturated fat consumption ( p = .005) and lower sodium ( p = .043) and sugar ( p = .038) intake. There were significant increases in leisure time physical activity ( p = .008). This study shows negative changes in sleep quality and improvements in diet and leisure time physical activity during the first 12 months of paramedic work. Individual differences observed in this study, and ongoing suboptimal dietary intake, highlight a need for larger studies with more participants and a focus on personalised strategies for new recruit paramedics.

Effects of Zeaxanthin on the Insulin Resistance and Gut Microbiota of High-Fat-Diet-Induced Obese Mice

Obesity-induced insulin resistance (IR) can precipitate metabolic disorders such as diabetes. Zeaxanthin, a crucial member of the carotenoid family, has been found to mitigate the damage caused by obesity. However, reports on the effects of zeaxanthin on obesity-induced IR are lacking. Our objective was to examine the metabolic regulatory impacts of zeaxanthin on mice subjected to a high-fat diet (HFD) that triggered IR and to explore their influence on gut microbiota regulation. This study constructed a mouse model of metabolic dysfunction caused by lipid-rich nutritional patterns to investigate physiological and biochemical indices, liver pathway expression, and the intestinal microbiota. The mechanisms by which zeaxanthin improved both IR and glucose metabolic disorders were elucidated. The results demonstrate that zeaxanthin effectively suppressed obesity. The fasting blood glucose, area under curve of oral glucose tolerance test and insulin tolerance test, and homeostatic model assessment–insulin resistance (HOMA-IR) indices in the HFDZEA group decreased by 14.9%, 25.2%, 28.9%, and 29.8%. Additionally, zeaxanthin improved the lipid metabolism and alleviated damage to the liver and pancreas while also activating the PI3K/Akt pathway, regulating hepatic gluconeogenesis and the glycogen metabolism. The number of OTUs in the HFDZEA group increased by 29.04%. Zeaxanthin improved the structure and profile of the gastrointestinal microbiome and enhanced its diversity, increasing probiotics abundance, decreasing pathogen abundance, and thereby ameliorating the dysbiosis of enteric microbial communities in rodents with obesity resulting from excessive fat consumption. The outcomes of our analysis provide a rational basis for advancing zeaxanthin-based nutritional products.

Preventing cardiovascular disease in at-risk patients: Results of a pilot behavioural health programme in general practice

Background The ‘High-Risk Prevention Programme’ (HRPP) involved a six-week health behaviour change programme based in general practices and aimed to address cardiovascular disease (CVD) risk in disadvantaged Irish communities. Objectives This pilot study aimed to establish the HRPP’s likely effectiveness and acceptability to inform the development of a future definitive trial. Methods The HRPP was conducted at six general practices in disadvantaged areas in the Ireland East region. Patients with high CVD risk were recruited by participating practices and were allocated to either a General Practice Nurse (GPN) or Health Promotion Professional (HPP) led programme focusing on positive health behavioural change. Baseline and 12-month follow-up data were collected to capture the HRPP’s likely effectiveness in promoting health outcomes and health behavioural change. Results The HRPP programme was completed by 270 patients. Out of these 270 patients, 245 (90.74%) completed baseline assessments, and 176 (65.19%) completed follow-up assessments at 12 months. Baseline data indicated a high level of CVD risk among patients and follow-up demonstrated positive change in several areas, especially weight (−1.95 kg, p < 0.001), BMI (−0.72, p < 0.001), exercise during the last week ( p <0.001), and consumption of healthy fats in the HPP group (+60%, p < 0.001). Conclusion The HRPP was a much-needed pilot intervention, and positive results were seen in both GPN and HPP arms, especially with regards to weight loss, exercise, and dietary improvements. Future definitive trials of the HRPP are likely to be effective and acceptable in terms of combatting these issues among high-risk patients.

Animal breeding and feeding tools may close human nutrition gaps

The last century has witnessed many innovations in agriculture and food technologies that have ensured the production of sufficient quantities of good hygienic food. Animal scientists have contributed substantially to efficient breeding and feeding practices by adapting animals for faster growth and improving feed efficiency and utilization. Breeding goals and feeding recommendations have been proposed with a primary focus on profitability to promote significant changes in the macronutrient content, i.e., animal body protein and fat reduction. However, improving the nutritional quality of meat was not included in the profit assessment. Increasing the lean meat fraction is consistent with the goal of public dietary guidelines for human nutrition proposed in 1980, emphasizing the importance of reducing the consumption of animal fat, particularly saturated fat. The application of breeding and feeding tools to modify and improve the fatty acid composition has been partly implemented in pigs and broiler chickens to supplement the dietary recommendations for humans. The health benefits of lean “red meat” have been questioned in recent years, with dietary guidelines and upper limits being introduced for human intake. Animal breeding indirectly reduces the total and heme iron (the redness generator) content in meat, due to covariation with priority breeding goals. Two micronutrients play important roles in the human diet and are derived largely from meat (selenium) and milk (iodine) if the amount provided and absorbed is sufficient and predictable. The iodine content can be highly dependent on the use of novel (more sustainable) feeds. The micronutrients discussed in this study (Fe, Se, I, and vitamin D) highlight opportunities for the utilization of breeding and feeding knowledge to adjust their levels to procure meat with a high nutrient density. The gaps in micronutrient levels in humans must be addressed by navigating within approved animal feeding levels. Animal scientists must recognize the nutritional impact of breeding and feeding and advertise them. In addition, human nutritionists must acknowledge the existing and potential changes in animal production to meet the dietary guidelines. Sustainable food production within the “One Health” concept can only be achieved through cooperation.

Erythrocyte membrane and breast milk fatty acid profile in lactating mothers: relationship with infant erythrocyte membrane fatty acid profile.

During the first thousand days of life, fetus and infant's nutrition depends on mother's diet. Polyunsaturated fatty acids(PUFA) are important substrates in infant neurogenesis. We related erythrocyte membrane (EM) and breast milk fatty acids (FA) profile in lactating mothers with the EM FA profile in exclusively breastfed infants and evaluated maternal fat consumption. We conducted an observational, cross-sectional analytical study. During the 2016-2019 period, milk and blood samples from adult mothers 90 days post-partum and infant's blood were analysed, and FA were determined by GC. A frequency of consumption survey of fatty acids precursor foods and sources was conducted. The sample included forty-five mother-infant EM and forty-five milk samples donated by the same mothers. A low percentage of DHA (0·14 (0·12-0·2)) was found in milk, consistent with mother's low consumption of DHA-rich foods. A significant positive correlation between infant's EM DHA percentage and milk DHA percentage (r = 0·39; P value 0·008), as well as between infant's EM ω-3 fatty acids sum and milk DHA percentage (r = 0·39; P value 0·008), was found. When milk had a DHA percentage greater than or equal to 0·20 %, infants had a significant increase in DHA in their EM. Mother's consumption of DHA precursors and sources was NS. The relation between the DHA percentage distribution found in maternal milk, and the DHA percentage distribution found in infant's and mother's EM was proven in this population. Dietary fatty acid intake is associated with the maternal milk lipid distribution and with mothers' and infant's EM fatty acids percentage.

Effect of Apple Puree as a Substitute for Fat and Sugar on the Texture and Physical Properties of Muffins

In the context of global public health concerns, reducing the fat and sugar content of baked goods has become a major priority. The excessive consumption of sugar and fat is associated with an increased risk of obesity, type II diabetes and cardiovascular disease. This study realizes the optimization of muffin formula by incorporating apple puree as a substitute for sucrose and fat, with the aim of increasing the nutritional value and reducing the caloric content of the product. A comprehensive analysis was performed to evaluate the impact of this substitution on various textural and physical properties of muffins. The firmness, chewiness, cohesiveness, height, volume and weight loss during baking of the muffins were determined. The result of the study showed that in order to obtain optimal textural and physical properties, the sugar and fat in the muffins can be substituted with applesauce in percentages of 34.04% and 43.78%, respectively. This research highlights the potential of apple puree to reduce the sugar and fat content and to lead to obtaining optimal physical and textural properties.

Evaluation of a Workplace Diabetes Prevention Program Delivered via Distance Learning: A Qualitative Study.

Little is known about how distance learning impacts delivery of the National Diabetes Prevention Program (DPP) despite a rapid shift to this platform during the coronavirus disease 2019 (COVID-19) public health emergency. We explored how a workplace DPP, delivered via distance learning, impacted knowledge, motivation, and behavioral skills of participants throughout the program. We conducted repeated qualitative interviews with distance learning participants at baseline, 6 months, and 12 months from September 2020 to July 2022. Three study team members coded interview data using individual responses as the unit of analysis. We used a thematic approach, using the information-motivation-behavioral skills framework, to analyze responses and generate understanding of the program's impact. The 27 individuals who participated in the interviews (89% women, mean age 56 years) reported the distance learning platform was effective in changing their behavior. The program's focus on food logging and setting limits on specific types of caloric intake was perceived as essential. Education on ideal levels of fat and sugar consumption, lessons on how to read food labels, and dissemination of recipes with healthy food substitutions allowed participants to initiate and sustain healthy decision-making. Strategies to increase physical activity, including breaking up exercise throughout the day, made reaching their goals more feasible. Participants reported food logging and weight reporting, as well as group support during sessions, either sustained or increased their motivation to adhere to the program over time. A workplace DPP delivered via distance learning successfully prompted improvements in the knowledge, motivation, and behavioral skills necessary to increase healthy eating and physical activity among participants.

7849 The EDC Mixture “NeuroMix” Has Developmental, Anxiolytic, And Obesogenic Effects In Rats

Abstract Disclosure: E. Morales-Grahl: None. L. M. Thompson: None. E.N. Hilz: None. A.C. Gore: None. Humans are exposed to a variety of endocrine disrupting chemicals (EDCs) on a daily basis through drinking water, food, and contact with everyday objects. Safety guidelines aim to keep exposure to individual EDCs below certain levels but don’t take into account the potentially unpredictable effects of simultaneous exposure to multiple EDCs. Furthermore, EDCs may exhibit non-monotonic dose responses. The present study assessed the effect of an EDC mixture at three different doses on two sets of behaviors implicated in EDC research: anxiety, and high fat and sugar consumption. Beginning in early pregnancy and until pups were weaned at day 21, Sprague Dawley rats were fed daily with NeuroMix, our lab’s EDC mixture containing 9 common industrial chemicals, at three doses (0.1x, 1x, 10x). The base dose (1x) contained EDCs below the no observed adverse effect level in humans (NOAEL). In adulthood, the female and male offspring were then subjected to an open field and light/dark test, followed by a high fat and high sucrose preference test. 0.1x NeuroMix exposure advanced the timing of vaginal opening in female offspring, while all doses increased anogenital distance in males, marking differences in sexual development. 1x NeuroMix exposure in male rats increased their time in the open field corners and their time immobile in the corners, indicating higher anxiety. No difference was seen in sucrose preference. Female rats exposed to 1x NeuroMix showed a higher preference for a high-fat diet, and gained more weight compared to the controls while on this diet. These findings demonstrate that a mixture of human-relevant chemicals, when administered perinatally to rats, have developmental, anxiogenic and obesogenic effects in a sexually dimorphic manner. We also show a non-monotonic response to our chemical mixture, with the 1x dose showing greater effects than the 10x dose. Overall, we provide evidence that even at levels considered safe for individual EDCs, there are adverse developmental and behavioral effects of EDCs when combined. Grant support: R35 ES035024, F32 ES034257. Presentation: 6/1/2024

Effectiveness of Poster, Animated Video and Live Youtube on Knowledge of Metabolic Syndrome and Sugar, Salt and Fat Consumption Recommendations (GGL)

Effectiveness of Poster, Animated Video and Live Youtube on Knowledge of Metabolic Syndrome and Sugar, Salt and Fat Consumption Recommendations (GGL)

Characterising healthy Australian oral microbiomes for ‘super donor’ selection

ObjectivesAmong healthy people, we understand very little about the sociodemographic, lifestyle, and dental hygiene behaviours that shape their oral microbiota. This study investigates how sociodemographic, lifestyle and dental hygiene behaviours shape oral microbiota diversity and composition in an Australian population to better inform healthy oral microbiota donors for Oral Microbiota Transplantation (OMT). MethodsThe study comprised 93 healthy people who underwent comprehensive oral examinations and questionnaires to assess their health status. Participants were excluded if they had any active systemic or oral disease. All completed a questionnaire containing information on socio-economic, lifestyle, behavioural, and oral health factors. Supragingival plaque was collected, and 16S ribosomal RNA (rRNA) amplicon sequencing was used to analyse microbial composition. Associations between the core microbiome, alpha- (within-sample), beta-diversity (between-sample) and an individual's co-variates were tested for statistical significance. A redundancy analysis (RDA), multivariate adonis, differential abundance and correlation analysis were performed to characterise which factors drive the variation in the healthy oral microbiome. ResultsStreptococcus and Corynebacterium were the most prevalent and abundant genera among healthy Australians. The alpha and beta diversity were higher among unemployed non-Australian-born students who consumed low carbohydrates, fat, and sugar and had not visited the dentist for over 12 months. Additionally, beta diversity was significantly higher among daily flossers who abstained from fluoride treatment and had high salivary pH, although no single factor explained more than 4% of the total variation (R2= 0.042). Alloprevotella, Lachnosporacea, and Parvimonas were significantly abundant among non-Australians who did not visit the dentist within a year. The RDA analysis revealed associations between microbiome composition and factors such as high carbohydrate, sugar, and fat consumption, low fibre intake, and regular dental checks among Australian-born individuals. ConclusionThese findings indicate that alpha and beta diversity of the oral microbiome varied significantly with sociodemographic, lifestyle, and dietary factors, including non-Australian birthplaces, unemployment, diet, and infrequent dental visits. Clinical SignificanceThese findings underscore the importance of considering diverse sociodemographic, lifestyle, and dietary factors in oral health management. Before microbiome transplantations, clinicians should account for individual characteristics that may be beneficial for shaping and maintaining optimal oral microbiome diversity and health.

Comparative analysis of high-fat diets: Effects of mutton, beef, and vegetable fats on body weight, biochemical profiles, and liver histology in mice

BackgroundHigh-fat diets are associated with metabolic syndrome, cardiovascular diseases, and liver disorders. Beef and mutton, both widely consumed meats, are significant sources of animal fat, while soybean oil, a commonly used cooking oil, is a prominent source of plant-derived fat. This study aimed to compare the effects of regular consumption of beef fat, mutton fat, and soybean oil in mice to assess potential health risks. MethodsSixty Swiss albino male mice were divided into four groups: a control group (Group A) fed a standard mice pellet, and three treatment groups (Groups B, C, D) receiving 10% dietary fat from mutton, beef, and soybean oil, respectively. Parameters such as body weight, caloric intake, serum markers, and liver histopathology were studied. ResultsConsumption of mutton fat, beef fat, or soybean oil supplemented diet in groups B, C, and D led to higher caloric intake and body weight compared to control group A, which received a standard diet. These diets also caused elevated serum glucose, impaired glucose tolerance, and increased triglycerides, cholesterol, LDL-C, and reduced HDL-C. Elevated AST and ALT levels in the high-fat diet groups, indicated liver damage and fat accumulation. Histological analysis confirmed steatosis, hepatocyte ballooning, and inflammation in all three high-fat diet groups, while the control group had normal liver histology. ConclusionHigh-fat diets, whether plant- or animal-based, led to weight gain in mice and resulted, poor glucose tolerance, dyslipidemia, liver damage and steatohepatitis. Further research is needed to explore the mechanisms behind these effects and improve understanding and management of high-fat diet consequences

Influence of multigenerational and living-alone households on high fat, sugar or sodium (HFSS) food consumption pattern in aging population

ObjectivesTo investigate the correlation between household composition and consumption of different groups of high fat, sugar or sodium (HFSS) among older persons in Thailand. Study DesignThis study used cross-sectional data from the 2021 population-based survey called the Health Behavior of Population Survey. MethodsData from this study were drawn from 39,384 sampled Thai older persons age 60 years or over. A food frequency questionnaire was used to collect information about frequency of HFSS food consumption, household composition, and socio-demographic characteristics. Binary logistic regression was performed to investigate correlations between household composition and food consumption. ResultsMore than three in five older Thais lived in a multigenerational home. The probabilities of consuming high-fat food, fast food, and snacks among older persons who lived with working-age person(s) and child(ren)/adolescents were much higher than for those living alone. Older persons who lived alone had the highest probability of consuming instant food, compared with other types of household composition. Participants who lived with child(ren)/adolescents only or with working-age person(s) only (p≤0.01) and child(ren)/adolescents only (p≤0.001) were more likely to consume sugar-sweetened beverages (SSB). ConclusionsThe findings point to life course interventions to discourage HFSS consumption across generations. Social interventions and public policy aiming at increasing intergenerational interactions could be beneficial for healthier diets for not only older persons but also across generations.

Infant diet quality index predicts nutrients of concern and ultra-processed food intake in low-income U.S. children

BackgroundDiet quality during infancy can influence nutrient intake and ultra-processed foods (UPF) consumption throughout later childhood. ObjectiveThis study investigated the predictive validity of Infant Diet Quality Index (IDQI) scores from 0-1 years of age and consumption of select nutrients and UPFs at different time points in low-income children ages 2-5 years. MethodsDietary surveys and 24-hour dietary recalls collected between ages 0-12 months from 2,613 Special Supplemental Women, Infants, and Children (WIC) Infant Toddler Feeding Practices Study-2 (ITFPS-2) participants were used to assess infant diet quality by final IDQI score ranging from 0 (non-adherence to dietary guidelines) to 1 (complete adherence to guidelines). Single 24-hour recalls collected across multiple time points per child between ages 2 and 5 years were used to determine nutrient intakes: vitamin B12 (μg), vitamin D (μg), calcium (mg), iron (mg), zinc (mg), potassium (mg), saturated fat (g), and dietary fiber (g), added sugars (g). Likewise, Nova was used to classify foods (to estimate the percentage of energy from foods) by level of industrial processing at each point in time across ages 2-5. Survey-weighted regression analyses estimated associations between total IDQI score and nutrient intake and percentage of energy consumption from each Nova food group at each age between 2-5 years. ResultsIDQI scores based on diet quality from 0-1 years of age were positively associated with children’s dietary fiber and potassium intake at ages 2-5. Additionally, IDQI was negatively associated with added sugar intake. No associations were observed between IDQI and saturated fat consumption. IDQI scores at age 1 were positively associated with the percentage of energy attributed to unprocessed/minimally processed foods (20% to 23%) and negatively associated with UPF consumption at ages 2-5 (-24% to -29%). ConclusionIDQI predicts intake of select nutrients and UPF consumption among low-income U.S. children ages 2-5. Clinical Trial RegistrationFeeding My Baby – A National WIC Study, NCT02031978, https://clinicaltrials.gov/study/NCT02031978. De-identified individual participant data from the WIC ITFPS-2 study is publicly available through USDA Ag Data Commons.

Anti-Inflammatory Oxysterol, Oxy210, Inhibits Atherosclerosis in Hyperlipidemic Mice and Inflammatory Responses of Vascular Cells.

We previously reported that Oxy210, an oxysterol-based drug candidate, exhibits antifibrotic and anti-inflammatory properties. We also showed that, in mice, it ameliorates hepatic hallmarks of non-alcoholic steatohepatitis (NASH), including inflammation and fibrosis, and reduces adipose tissue inflammation. Here, we aim to investigate the effects of Oxy210 on atherosclerosis, an inflammatory disease of the large arteries that is linked to NASH in epidemiologic studies, shares many of the same risk factors, and is the major cause of mortality in people with NASH. Oxy210 was studied in vivo in APOE*3-Leiden.CETP mice, a humanized mouse model for both NASH and atherosclerosis, in which symptoms are induced by consumption of a high fat, high cholesterol "Western" diet (WD). Oxy210 was also studied in vitro using two cell types that are important in atherogenesis: human aortic endothelial cells (HAECs) and macrophages treated with atherogenic and inflammatory agents. Oxy210 reduced atherosclerotic lesion formation by more than 50% in hyperlipidemic mice fed the WD for 16 weeks. This was accompanied by reduced plasma cholesterol levels and reduced macrophages in lesions. In HAECs and macrophages, Oxy210 reduced the expression of key inflammatory markers associated with atherosclerosis, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), chemokine (C-C motif) ligand 2 (CCL2), vascular cell adhesion molecule-1 (VCAM-1), and E-Selectin. In addition, cholesterol efflux was significantly enhanced in macrophages treated with Oxy210. These findings suggest that Oxy210 could be a drug candidate for targeting both NASH and atherosclerosis, as well as chronic inflammation associated with the manifestations of metabolic syndrome.

Differences in Diet Assessment and Body Composition among Young Spanish Elite Footballers: Morning Training vs. Evening Training

The training schedule is a factor that influences sports performance optimization. In a sport like soccer, there is often significant disparity in training schedules among different teams within the same club, without considering whether this may affect players’ performance. The aim of this study was to describe differences in nutrient intake and body composition in elite youth soccer players from the Spanish league with different training schedules (morning and evening). A cross-sectional study was conducted to determine differences in anthropometric variables and dietary assessment in a sample of Spanish young soccer players. A total of 41 players participated in this study. After comparing the groups according to their training schedule, no differences were observed in body composition between both groups; the evening-night training group showed higher consumption of lipids and saturated fats. In summary, more experimental studies are needed to determine the effects on various health and performance parameters of different training schedules in young population.

Fish oil-based bigels with outstanding sensory and antioxidant properties: Application in low-fat mayonnaise

Low-fat mayonnaise has gained widespread popularity as a condiment, driven in part by the global rise in obesity rates, which is partially linked to excessive fat consumption. In this study, the bigel systems were designed by preparing fish oil oleogels and gelatin hydrogels to produce low-fat mayonnaise. As the oleogel content increased in bigels, a phase inversion shifted from oil-in-water (O/W) systems to water-in-oil (W/O) systems. Rheology and mechanical property analysis revealed that the hardness and gel strength of bigels improved as the oleogel content increased. The freeze-thaw stability of bigels was impacted by the ratio of oil and water phases, with O/W systems enduring one additional freeze-thaw cycle compared to W/O systems. The bigel containing 40% oleogel (BG 40) and lemon essential oil significantly enhanced antioxidant properties, as measured by the levels of primary and secondary oxidation products. The antioxidative capacity of the gel, as indicated by the reduced development of primary and secondary oxidation products, was 301.4% and 196.7% greater, respectively, compared to that of the pure oleogel. Based on magnetic resonance imaging and sensory rating analysis, sample BG 40 demonstrated the most homogeneous structure and the best sensory properties. The sample BG 40, used as the fat substitute in preparing low-fat mayonnaise, reduced total oil content compared to full-fat mayonnaise. Our findings contributed to the development of low-fat mayonnaise using fish oil-based bigels, which exhibit exceptional sensory and antioxidant properties.

Pengaruh Substitusi Tepung Ikan dengan Tepung Larva BSF dalam Ransum terhadap Konsumsi Protein Kasar, Lemak Kasar dan Serat Kasar pada Burung Puyuh

The research was carried out experimentally at the Non-Ruminant Livestock Laboratory, Faculty of Animal Husbandry, Hasanuddin University from April to June 2019. This research aims to determine the effect of substituting fish meal with BSF larvae meal in the ration on the consumption of crude protein, crude fat and crude fiber in quail. 60 female quail were used in this study using cages measuring 90 x 60 x 30 cm. This research method used a completely randomized design (CRD) consisting of four treatments and five replications, divided randomly based on treatment as follows: P0 Ration without adding BSF larval meal, P1 = BSF larval meal 3.183% (6.67% crude fish meal protein) , P2=BSF larval meal 6.370% (3.34% fish meal crude protein), P3=BSF larval meal 9.565% (0% fish meal crude protein). The variables observed were the consumption of crude protein, crude fat and crude fiber in quail. The data obtained were then analyzed using IBM SPSS Statistics 25 software. The results of the analysis of the effect of substituting fish meal with BSF larvae meal in the ration had no significant effect (P&gt;0.05) on the consumption of crude protein, crude fat and crude fiber in quail.