Objective To investigate the action mechanism of fat atypical cadherin 1 (FAT1) regulating invasion by targeting hypoxia inducible factor-1α (HIF-1α) in high grade glioma under hypoxia. Methods The expression levels of FAT1 and HIF-1α in 48 cases of grade Ⅲ-Ⅳ glioma athological tissue samples in our hospital were detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). The relationship between the expression of FAT1 and HIF-1α was analyzed. The expression of FAT1 and HIF-1α in human glioma cells U87MG and GOS3 under the condition of normal oxygen (20% O2) and hypoxia (1% O2) was detected by FQ-PCR. FAT1 small interfering RNA (siRNA), HIF-1α siRNA and control siRNA were transfected into U87MG and GOS3 cell lines respectively by Lipofectamine 2000. The expression of FAT1 and HIF-1 under the normal oxygen and hypoxia conditions was detected. The invasive abilities of U87MG and GOS3 glioma cells were examined by cell chamber technique. Results The relative expression levels of FAT1 and HIF-1α in 48 cases of pathological tissues of patients with grade Ⅲ-Ⅳ glioma were 4.07 (0.72, 13.42) and 0.94 (0.29, 3.49) respectively. Spearman analysis showed that there was significant correlation between the relative expression levels of FAT1 and HIF-1α (r=0.835, P=0.000). The normal oxygen concentration in GOS3 cell lines FAT1 (0.29±0.03) and HIF-1α (0.34±0.09) relative expression levels were significantly lower than that of U87MG cells (1.08±0.15, 1.00±0.12; t=8.945, 7.621; P=0.010, 0.002); hypoxia condition, U87MG and GOS3 cell lines FAT1 and HIF-1α relative expression levels were significantly higher than the normal oxygen concentration (t=17.994, 8.920, 14.047, 4.037; P=0.000, 0.001, 0.004, 0.016); U87MG cells under hypoxic conditions FAT1 (4.38±0.28) and HIF-1α (2.76±0.32) relative expression levels were significantly higher than that of GOS3 cell line (1.69±0.17, 0.83±0.19; t=14.224, 8.982; P=0.000, 0.001). After transfection of FAT1 siRNA, FAT1 (0.17±0.06, 0.16±0.03, 0.16±0.03, 0.18±0.03) and HIF-1α (0.18±0.03, 0.17±0.04, 0.15±0.04, 0.21±0.02) relative expression levels of U87MG and GOS3 cells in the normal oxygen and hypoxia were significantly decreased (t=7.577, 7.793, 7.793, 7.783, 9.285, 9.260, 9.484, 9.042; P=0.002, 0.013, 0.013, 0.014, 0.009, 0.007, 0.007, 0.011). After transfection of HIF-1α siRNA, FAT1 (0.16±0.04, 0.28±0.03) relative expression levels in the normal oxygen and HIF-1α (0.15±0.03, 0.16±0.02, 0.15±0.03, 0.17±0.02) relative expression levels of U87MG and GOS3 cells in normal oxygen and hypoxia conditions were significantly decreased (t=7.890, 6.834, 9.624, 9.614, 9.624, 9.500; P=0.012, 0.018, 0.008, 0.009, 0.008, 0.010); but the level under hypoxic conditions relative expression of FAT1 (4.27±0.41, 2.72±0.25) were significantly higher than that in normal oxygen conditions (t=12.649, 9.671; P=0.000, 0.001). Under hypoxic conditions, the invasion cell quantity of U87MG cells transfected with FAT1 siRNA and HIF-1α siRNA [(23.2±4.2) and (21.5±3.1) cells] were lower than those of normal oxygen conditions [(57.6±8.1) and (58.7±7.6) cells; t=6.530, 7.850; P=0.003, 0.001]. Conclusion The expression of FAT1 and HIF-1 in high grade gliomas is strongly correlated, and the expression is high under the hypoxia condition. FAT1 can promote the invasion of glioma cells by targeting HIF-1α. Key words: Glioma; Fat atypical cadherin; Hypoxia inducible factor-1α; Invasion
Read full abstract