Fasting Blood Glucose Test Research Articles

Effect of long term treatment with antiepileptic drugs on carotid intima-media thickness as an indicator of atherosclerosis

Purpose: The aim of current study was to investigate the possible relation between antiepileptic drugs (AEDs) and atheosclerotic risk factors and carotid intima media thickness (IMT) as a marker of atherosclerosis. Materials and Methods: Ninety-two patients, who had used carbamazepine (CBZ) and valproic acid (VPA) for three or more years and who had used a second AED (polytherapy) for two years in addition to CBZ or VPA monotherapy, were included in the study. The control group was composed of 31subjects with similiar demographics. Fasting blood glucose, liver and renal function tests, lipid profile, homocysteine, serum high sensitivity C-Reactive Protein (Hs-CRP), uric acid (UA), lipoprotein (a) (Lp (a)), folate and vitamine B12 levels were examined in both groups. Results: Homocysteine levels were higher in the VPA, CBZ and politherapy groups compared to the control group. UA levels were higher in the VPA monotherapy group when compared to the CBZ monotherapy and polytherapy group. In the CBZ monotherapy group, Hs-CRP levels were higher than in the VPA monotherapy, polytherapy and control groups. Total cholesterol levels were higher in the CBZ monotherapy group when compared to the VPA monotherapy and control groups. Conclusion: The findings showed no correlation between IMT values and duration of disease, duration of drug usage and dose, which suggests that long-term AED usage does not increase the development of atherosclerosis.

Prevalence and risk factors for type 2 diabetes mellitus in women with gestational diabetes mellitus: a systematic review and meta-analysis

IntroductionThis study aims to explore the risk factors in the progression of gestational diabetes mellitus (GDM) to type 2 diabetes mellitus (T2DM).Material and methodsRelevant studies were comprehensively searched from PubMed, Web of Science, Cochrane Library, and Embase up to March 12. Data extraction was performed. Differences in risk factors were presented as odds ratios (OR) and corresponding 95% confidence intervals (CI). The quality of the included studies was assessed through the Newcastle-Ottawa Scale and the Agency for Healthcare Research and Quality scale.ResultsThis meta-analysis encompassed 46 studies involving a total of 196,494 patients. The factors most strongly associated with the risk of developing T2DM following GDM were the use of progestin-only contraceptives (odds ratio [OR]: 2.12, 95% confidence interval [CI] = 1.00–4.45, P = 0.049), recurrence of GDM (OR: 2.63, 95% CI = 1.88–3.69, P < 0.001), insulin use during pregnancy (OR: 4.35, 95% CI = 3.17–5.96, P < 0.001), pre-pregnancy body mass index (BMI) (OR: 2.97, 95% CI = 2.16–4.07, P < 0.001), BMI after delivery (OR: 4.17, 95% CI = 2.58–6.74, P < 0.001), macrosomia (OR: 3.30, 95% CI = 1.45–7.49, P = 0.04), hypertension (OR: 5.19, 95% CI = 1.31–20.51, P = 0.019), and HbA1c levels (OR: 3.32, 95% CI = 1.81–6.11, P < 0.001). Additionally, age (OR: 1.71, 95% CI = 1.23–2.38, P = 0.001), family history of diabetes (OR: 1.47, 95% CI = 1.27–1.70, P < 0.001), BMI during pregnancy (OR: 1.06, 95% CI = 1.00–1.12, P = 0.056), fasting blood glucose (FBG) (OR: 1.58, 95% CI = 1.36–1.84, P < 0.001), 1-hour oral glucose tolerance test (OGTT) (OR: 1.38, 95% CI = 1.02–1.87, P = 0.037), and 2-hour OGTT (OR: 1.54, 95% CI = 1.28–1.58, P < 0.001) were identified as moderate-risk factors for the development of T2DM.ConclusionThe systematic review and meta-analysis identified several moderate- to high-risk factors associated with the progression of T2DM in individuals with a history of GDM. These risk factors include the use of progestin-only contraceptives, pre-pregnancy BMI, BMI after delivery, macrosomia, hypertension, persistently elevated levels of HbA1c, fasting blood glucose (FBG), 1-hour and 2-hour oral glucose tolerance tests (OGTT), age, and family history of diabetes. Our findings serve as evidence for the early prevention and clinical intervention of the progression from GDM to T2DM and offer valuable insights to guide healthcare professionals in formulating customized management and treatment strategies for female patients with diverse forms of GDM.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024545200.

Elevated glucagon and postprandial hyperglycemia in fatty liver indicate early glucose intolerance in metabolic dysfunction associated steatotic liver disease

In Japan, fatty liver cases with elevated body mass index are increasing. Because of being non-malignant, these are often neglected unless accompanied by diabetes. This study investigated the risk of glucose intolerance in individuals with non-alcoholic fatty liver. We included 165 men (mean age 56.3 years; range 29–75 years) who underwent an overnight 2-day physical examination at our Health Evaluation Center. All patients underwent abdominal ultrasonography to examine fatty liver. Fasting blood glucose and 75-g glucose tolerance test (OGTT) were conducted. alanine aminotransferase (ALT) (p < 0.01) and triglyceride (p < 0.001) levels were significantly higher in the fatty liver group (FL) than in the non-fatty liver group. HbA1c, fasting blood glucose, and blood glucose level at OGTT (0 and 30 min) did not show significant differences. In the FL, OGTT was significantly elevated at 60 min(p < 0.01)and 120 min (p < 0.001), insulin level was significantly elevated at 0 and 30 min (p < 0.001), and glucagon level was significantly elevated at 0 min (p < 0.05) and 30 min (p < 0.01), with no significant differences between the groups at 60 and 120 min. This is the first study to demonstrate elevated glucagon levels after OGTT. Metabolic dysfunction-associated steatotic liver disease (MASLD) requires treatment for insulin resistance with glucagon dysregulation likely associated with its pathogenesis.

Hubungan Diabetes Melitus Tipe 2 dengan Derajat Obstruksi Penyakit Paru Obstruktif Kronik (PPOK) di RS. Royal Prima Periode 2022-2023

Type 2 Diabetes Mellitus (T2DM) is one of the most common comorbidities in patients with Chronic Obstructive Pulmonary Disease (COPD). COPD itself can be a risk factor in the development of T2DM through systemic inflammatory mechanisms caused by continuous and progressive exposure to cigarette smoke over time. To investigate the relationship between diabetes mellitus and the severity of COPD obstruction based on demographic data, spirometry test results, and fasting blood glucose (FBG) test results. This study used a quantitative method with an observational descriptive design. Sampling was done using total sampling technique with a cross-sectional survey design and a retrospective approach using secondary data. Out of 31 study subjects diagnosed with COPD and T2DM, 83.9% (26 subjects) were male and 16.1% (5 subjects) were female. In terms of age distribution, the 40–59-year age group had the highest frequency, totaling 18 individuals (58.1%). In terms of occupation, self-employed individuals represented the largest group with 24 subjects (77.4%). For COPD severity, the highest frequency was found in the severe COPD category, with 15 subjects (48.4%). Based on the results of the study investigating the relationship between Type 2 Diabetes Mellitus and the severity of COPD obstruction, a significant relationship was found between T2DM and COPD obstruction severity when analyzed by gender, age, occupation, and the results of spirometry and fasting blood glucose tests.

Effect of moderate-intensity aerobic exercise on bladder TGF-β1 and type I collagen expressions in diabetic rat model.

Transforming growth factor-beta 1 (TGF-β1) and type I collagen play crucial roles in the pathogenesis of diabetic bladder disease (DBD). Moderate-intensity aerobic exercise increases antioxidant activity to help manage DBD. The aim of this study was to evaluate the effect of moderate-intensity aerobic exercise on the expression of TGF-β1 and type I collagen in the detrusor and lamina propria of the bladder in a type 2 diabetes mellitus (T2DM) rat model. A true experimental design with a post-test-only control group design was conducted with white rats (Rattus norvegicus), divided into three groups: a T2DM model group sacrificed after T2DM induction and diagnosed with T2D from a fasting blood glucose (FBC) test (Group C), a T2DM model group that did not receive exercise (Group NE), and a T2DM model group that received moderate-intensity aerobic exercise (Group E). Moderate-intensity aerobic exercise was conducted over six weeks, with a frequency of five days per week for 60 minutes per session. The findings revealed a significant reduction in TGF-β1 expression in the lamina propria in Group E compared to Group C (p = 0.004) Additionally, both Group E (p = 0.002) and Group NE (p = 0.028) showed a significant reduction in type I collagen expression in the lamina propria compared to Group C. These findings provide a basis for further investigation regarding the mechanism of non-pharmacologic DBD management by employing moderate- intensity exercise.

Defects of WFS1-mediated peptide hormones secretion contribute to the manifestations of Wolfram syndrome

AimsThe study aims to investigate whether WFS1 is involved in the regulation of the exportation and secretion of other peptide hormones, as well as to elucidate the precise molecular mechanisms underlying WS caused by pathogenic mutations in the WFS1 gene. Materials and methodsThe plasma proteome from the WS patients (n = 2, male) and WFS1-deficient mice (n = 5, male) were analyzed using liquid-chromatography tandem mass spectrometry (LC-MS/MS), while age- and gender-matched healthy individuals and wildtype (WT) mice serve as controls. WFS1-deficient mice were intraperitoneally injected with IGF1 starting from 4 weeks of age. Body weight was monitored every 2 days, fasting blood glucose and glucose tolerance test were performed on the day 30 and day 40 after injection of IGF1, respectively. BiFC (bimolecular fluorescence complementation) and Co-immunoprecipitation (IP) were used to analyze the interaction between WFS1 and peptide hormones. Confocal microscopy was employed to analyze the colocalization of IGF1 with ER and Golgi. Key findingsPeptide hormones are deficient in both the plasma of WS patients and WFS1-deficient mice. WFS1 binds to and mediates the secretion of these peptide hormones, suggesting that WFS1 serves as a general COPII vesicular receptor for sorting peptide hormones. Interestingly, the WFS1 pathogenic mutations significantly disrupt its interaction with these peptide hormones. Furthermore, intraperitoneal administration of IGF1 partially attenuates high blood glucose levels in WFS1-deficient male mice. SignificanceThis study suggests that WS is characterized by defective peptide hormone secretion and proposes administration of these deficient peptide hormones as a promising treatment regimen for WS.

Cutoff Values for Glycated Albumin, 1,5-Anhydroglucitol, and Fructosamine as Alternative Markers for Hyperglycemia.

Glycated albumin (GA), 1,5-anhydroglucitol (1,5-AG), and fructosamine have attracted considerable interest as markers of hyperglycemia. This study aimed to evaluate the optimal cutoff values for GA, 1,5-AG, and fructosamine and to determine their respective diagnostic efficacies in relation to hyperglycemia. We enrolled 6012 individuals who had undergone fasting blood glucose (FBG) and Hemoglobin A1c (HbA1c) tests along with at least one alternative glycemic marker. Receiver operating characteristic (ROC) curves and the upper or lower limit of the reference range (97.5 or 2.5 percentiles) were used to ascertain the optimal cutoff values. Follow-up data from healthy individuals were used to identify patients who developed diabetes mellitus (DM). The ROC cutoff values for GA, 1,5-AG, and fructosamine were 13.9%, 13.3 μg/mL, and 278 μmol/L, respectively, with corresponding area under the curve (AUC) values of 0.860, 0.879, and 0.834. The upper limits of the reference intervals for GA and fructosamine were 15.1% and 279 μmol/L, respectively, and the lower limit for 1,5-AG was 5.3 μg/mL. Among the GA cutoff values, the ROC cutoff had the highest sensitivity. Analyzing the follow-up data showed that lowering the GA cutoff from 16.0% to 13.9% identified an additional 40 people with DM progression. Lowering the GA cutoff values significantly increased the sensitivity of DM diagnosis and enhanced its potential as a screening marker by identifying more individuals with diabetes progression. Conversely, modifications to the cutoff values for 1,5-AG and fructosamine did not confer any discernible diagnostic or predictive advantages.

Attitude, Knowledge, and Practices Regarding Prediabetes in Saudi Arabia: A Cross-Sectional Study on Family Medicine Residents.

Background Individuals with prediabetes are at an increased risk of developing type 2 diabetes mellitus (T2DM) and cardiovascular diseasesand mortality from any cause. The treatment and early detection of prediabetes and T2DM can aid in the prevention of future health complications. The aim of this research was to assess the attitude, knowledge, and practices of family medicine (FM) residents in Saudi Arabia regarding prediabetes. Methodology This is an online cross-sectional survey study that was conducted between March and May 2023. The study population consisted of family medicine residents who are currently practicing their profession in Saudi Arabia. Results A total of 101 family medicine residents participated in this study. The study participants showed a positive attitude towardprediabetes management with a mean score of 25.4 (standard deviation {SD}: 4.8) out of 30. The study participants showed a moderate level of knowledge regarding prediabetes management with a mean score of 7.0 (SD: 2.2) out of 12. The proportion of participants who were able to identify risk factors that might prompt them to screen for diabetes mellitus ranged from 47.5% (n = 48) to 96.0% (n = 97). The participants' agreement levels for questions that examined their attitude towardthe importance of prediabetes screening ranged from 73.3% (n = 74) to 90.1% (n = 91). The most commonly agreed upon barrier was the patient's lack of motivation (n = 80, 79.2%). The most commonly agreed upon interventions that facilitate management and treatment improvements of prediabetes were more resources for patient education and increased access to the diabetes prevention program (DPP), an evidence-based lifestyle change program (n = 80, 79.2%). A body mass index (BMI) of ≥35 kg/m2 was the most commonly reported factor that increases the likelihood of prescribing metformin for a patient with prediabetes. The most commonly agreed upon barrier to prescribing metformin for prediabetes patients was that patients do not like taking medications (n = 67, 66.3%). Conclusions The study participants demonstrated an average level of understanding and a positive attitude regarding the management of prediabetes. A significant percentage of the participants demonstrated comprehension of risk factors associated with diabetes, as well as appropriate screening methods, with fasting blood glucose tests being of particular interest. It is recommended to enhance prediabetes management through heightened awareness and education concerning screening methodologies and lifestyle modifications.

Assessment of burden of prediabetes and diabetes with oral glucose tolerance test in community-based settings of Bengaluru rural district.

Diabetes is a major public health concern in India. The majority of studies done in rural population lack systematic methodology in estimating the prevalence of diabetes mellitus. Objectives of this study were to estimate the burden of prediabetes, type 2 diabetes mellitus and its associated risk factors among adults (18-69) years in rural Bengaluru, Karnataka. A cross-sectional study was conducted among adults aged 18-69 years in three villages of Devanahalli taluk of rural Bengaluru between May and October 2019. Behavioural and metabolic risk factors related to diabetes were assessed using WHO-STEPS instrument and IDSP-NCD risk factor tool. Fasting blood glucose and OGTT test were performed, and WHO diagnostic criteria were used to diagnose diabetes mellitus. Prevalence of prediabetes and type 2 diabetes (T2DM) in rural Bengaluru was found to be 6.3% and 10.9%, respectively. Behavioural risk factors related to diabetes measured in the study population were current tobacco use (32.7%), current alcohol use (9.9%), physical inactivity (46.8%) and unhealthy diet (82.3%). Metabolic risk factors measured were obesity (7.5%), central obesity (46.4%), prediabetes (6.3%) and hypertension (24.3%). More than one-thirds of diabetes and three-fourths of hypertension individuals were newly diagnosed by the population-based screening. The burden of diabetes and its risk factors are high due to the health transition occurring in rural population of Bengaluru. Undiagnosed diabetes mellitus and hypertension in rural population as demonstrated by this study highlight the need to strengthen the population-based screening and diagnosis of T2DM and hypertension (HTN) under the national health programme.

Use of the Screening for Occult Renal Disease (SCORED) Questionnaire to Screen Diabetic Nephropathy in Asymptomatic Patients: A Cross-Sectional Study.

The 2007 Screening for Occult Renal Disease (SCORED)questionnaire accesses risk factors for chronic kidney disease (CKD) and makes it possible to screen high-risk patients, being adapted and validated for the Brazilian culture in 2012. The present study evaluated the questionnaire's ability to predict the occurrence of CKD in asymptomatic patients, as well as identify a high risk for developing the disease. This was an analytical observational study with a cross-sectional design carried out in two stages: answering the SCORED and performing fasting blood glucose and creatinine tests. The participants were patients at the Hospital das Clínicas of the Federal University of Minas Gerais (HC-UFMG) with scheduled creatinine and fasting blood glucose tests, respecting the inclusion and exclusion criteria defined for the study. SCORED was applied with questions covering gender, age, proteinuria, and diabetes, being classified as high or low risk for CKD. The data collected were height, weight, age, sex, diagnosis of diabetes mellitus, and fasting blood glucose. The sample space was 212 individuals, the majority of whom were female (N=130, 61.3%), with a median of 58 years of age. The prevalence of CKD was 15.6% (N=33) with a sensitivity of 90.9%, a specificity of 30.2%, a positive predictive value of 19.4%, a negative predictive value of 94.7%, and a diagnostic accuracy of 39.7%. We concluded that the SCORED questionnaire can be a useful tool for screening CKD in asymptomatic patients and also that there is a relationship was detected between glycemic changes and an increased risk for CKD.

Neuroprotective Effects of Bexarotene and Icariin in a Diabetic Rat Model.

Objective Type 2 diabetes mellitus (T2DM), a chronic metabolic disorder affecting over 400 million people globally, is increasingly recognized for its detrimental impact on the central nervous system. T2DM is linked to neurodegenerative diseases like Alzheimer's and vascular dementia. This study investigates the neuroprotective effects of bexarotene and icariin in a T2DM rat model, focusing on brain-derived neurotrophic factor (BDNF), glial fibrillary acidic protein (GFAP), and neurofilament-light chain (NfL) levels. Methods Before the study, rats underwent fasting blood glucose tests, lipid profile assessments, and general health evaluations, followed by a high-fat diet for two weeks and a single streptozotocin dose (35 mg/kg). Rats with fasting blood glucose levels ≥250 mg/dl were classified as diabetes mellitus (DM) and continued on the high-fat diet throughout the experiment. Forty-seven male Wistar Albino rats were divided into six groups: a healthy control group, a DM control group, a DM group treated with bexarotene, a DM group treated with icariin, and two DM groups treated with combinations of low and high doses of bexarotene and icariin. After the 45-day treatment, blood samples were collected under thiopental sodium anesthesia, with HbA1c (glycosylated hemoglobin) and hematological parameters analyzed within eight hours, and serum stored at -80°C for further analysis. The animals were then euthanized, and brain tissues were harvested, frozen, and stored at -80°C until further examination. Brain tissues were analyzed for BDNF, GFAP, and NfL levels using ELISA (enzyme-linked immunosorbent assay). For comparing multiple groups, the Kruskal-Wallis test was applied to nonparametric data, and one-way ANOVA was used for parametric data, followed by Bonferroni's post hoc test for pairwise comparisons. Statistical significance was determined with two-tailed tests at p < 0.05. Results Significant changes in GFAP levels were observed across groups (p < 0.001). The DM control group showed the highest GFAP levels, while treatment groups exhibited reductions. The DM control group also showed the highest BDNF levels, while treatment groups exhibited reductions. The DM control group showed the lowest NfL levels, while treatment groups exhibited increments. Conclusion This study highlights the neuroprotective potential of bexarotene and icariin in a diabetic rat model, evidenced by significant changes in GFAP levels. The lack of significant changes in BDNF and NfL suggests that longer study durations may be necessary to observe these effects. Future research should include extended study periods, larger sample sizes, varied dosages, and comprehensive behavioral assessments to better understand the therapeutic potential of these agents.

Anti-hyperglycemic effect of Commelina diffusa extracts on diabetic mice model

This study aimed to investigate the blood glucose stabilising effect of extracts from Commelina diffusa Burm.f. on glucose tolerance test and diabetes test in white mice by streptozotocin. The diabetic mice were induced to hyperglycemia by intraperitoneal injection of streptozotocin in a single dose (170 mg/kg). Water extract, 96% ethanol extract, and 50% ethanol extract of C. diffusa were given orally for 7 days and the reference drug glibenclamide was given orally once on the 7th experimental day. The hypoglycemic effect was evaluated by quantifying fasting plasma glucose concentration and glucose tolerance test. The results of 96% ethanol extract of C. diffusa at two oral doses of 0.21 and 0.42 g/kg showed a more typical hypoglycemic effect than the water extract (0.36 and 0.72 g/kg) and 50% ethanol extract (0.49 and 0.98 g/kg) in both fasting blood glucose and glucose tolerance tests. The hypoglycemic effect of 96% ethanol extract of C. diffusa was equivalent to glibenclamide at a dose of 5 mg/kg measured in fasting state and was weaker than glibenclamide in the glucose tolerance test.

A novel predictive model for optimizing diabetes screening in older adults.

The fasting blood glucose test is widely used for diabetes screening. However, it may fail to detect early-stage diabetes characterized by elevated postprandial glucose levels. Hence, we developed and internally validated a nomogram to predict the diabetes risk in older adults with normal fasting glucose levels. This study enrolled 2,235 older adults, dividing them into a Training Set (n = 1,564) and a Validation Set (n = 671) based on a 7:3 ratio. We employed the least absolute shrinkage and selection operator regression to identify predictors for constructing the nomogram. Calibration and discrimination were employed to assess the nomogram's performance, while its clinical utility was evaluated through decision curve analysis. Nine key variables were identified as significant factors: age, gender, body mass index, fasting blood glucose, triglycerides, alanine aminotransferase, the ratio of alanine aminotransferase to aspartate aminotransferase, blood urea nitrogen, and hemoglobin. The nomogram demonstrated good discrimination, with an area under the receiver operating characteristic curve of 0.824 in the Training Set and 0.809 in the Validation Set. Calibration curves for both sets confirmed the model's accuracy in estimating the actual diabetes risk. Decision curve analysis highlighted the model's clinical utility. We provided a dynamic nomogram for identifying older adults at risk of diabetes, potentially enhancing the efficiency of diabetes screening in primary healthcare units.

Fasting blood glucose as a screening measure for late-onset gestational diabetes in the third trimester.

To investigate the positive rate of late-onset gestational diabetes mellitus (GDM) by additional fasting blood glucose (FBG) screening at 32-34 gestational weeks (GW) and analyse the perinatal outcomes of late-onset GDM after standard treatment. An Prospective cohort study. Single centre in China. 1130 singleton pregnancies with negative GDM screening in their first and second trimester. Additional FBG testing was performed at 32-34 GW. Pregnancies with FBG ≥5.1 mmol/L were diagnosed as GDM and received standardized treatment. Perinatal outcomes were collected and compared. Diagnosis of late-onset GDM, obstetric and neonatal outcomes. 6.3% (71/1130) of participants had FBG values ≥5.1 mmol/L and were diagnosed with late-onset GDM. Sixty-five (91.5%) were treated by dietary therapy and 6 (8.5%) by insulin therapy. The perinatal outcomes of full-term delivery were compared. The incidence of macrosomia (22.7% vs. 5.1%, adjusted odds ratio (aOR) 5.51, 95% confidence interval (CI) 1.83-16.61, p = 0.002) and NICU transferring (18.3% vs. 10.1%, aOR 1.94, 95% CI 1.01-3.74, p = 0.046) was significantly higher in late-onset GDM group than that in FBG <5.1 mmol/L group. Elevated FBG was associated with overweight or obesity during pregnancy (54.9% vs. 34.9%, OR 2.27, 95% CI 1.40-3.68, p = 0.001). 6.3% of singleton pregnancies with normal GDM screening results in the first and second trimester were found to have late-onset GDM by additional FBG screening at 32-34 GW, and their risk of macrosomia during a full-term pregnancy remains significantly higher after standard treatment.

Risk assessment of diabetes among undergraduate students of Northern Maharashtra using Indian Diabetes Risk Score and its correlation with fasting blood glucose

Abstract Background: Diabetes, once primarily associated with old age, is increasingly prevalent among young adults. The Indian Diabetes Risk Score (IDRS) employs four parameters—age, waist circumference, family history of diabetes, and physical activity—to screen for diabetes. This study aims to evaluate the risk and prevalence of diabetes and prediabetes in undergraduate medical students and to correlate these findings with their fasting glucose levels. Materials and Methods: A cross-sectional descriptive study was carried out among 336 undergraduate medical students in the Jalgaon district of Maharashtra. Data on socio-demographic and anthropometric factors were gathered using a structured, pre-tested questionnaire. Each participant’s IDRS score was calculated. Students were then invited to have their fasting blood glucose levels measured after an overnight fast. Results: The study surveyed 336 medical students, comprising 185 males (55.06%) and 151 females (44.94%). According to the IDRS, 5 students (1.49%) were categorized as high risk, 202 students (60.1%) as moderate risk, and 129 students (38.4%) as low risk for diabetes. Fasting blood glucose tests indicated that 3 students (0.90%) had impaired glucose tolerance, and 52 (15.48%) students were pre-diabetic. There was a statistically significant correlation between fasting blood glucose levels and IDRS (R = 0.466, P &lt; 0.001). Conclusion: The risk of diabetes among the participating undergraduate students is alarmingly high, according to the IDRS score. The strong correlation between the IDRS and the blood glucose levels of the study participants demonstrates that the IDRS is a highly effective, cost-efficient tool for predicting diabetes risk.

Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes

BackgroundThe number of patients with type 1 diabetes rises rapidly around the world in recent years. Maternal diabetes has a detrimental effect on reproductive outcomes due to decreased oocyte quality. However, the strategies to improve the oocyte quality and artificial reproductive technology (ART) efficiency of infertile females suffering from diabetes have not been fully studied. In this study, we aimed to examine the effects of nicotinamide mononucleotide (NMN) on oocyte maturation of mouse with type 1 diabetes mouse and explore the underlying mechanisms of NMN’s effect.MethodsStreptozotocin (STZ) was used to establish the mouse models with type 1 diabetes. The successful establishment of the models was confirmed by the results of body weight test, fasting blood glucose test and haematoxylin and eosin (H&E) staining. The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined. Immunofluorescence staining (IF) was performed to examine the reactive oxygen species (ROS) level, spindle/chromosome structure, mitochondrial function, actin dynamics, DNA damage and histone modification of oocytes, which are potential factors affecting the oocyte quality. The quantitative reverse transcription PCR (RT-qPCR) was used to detect the mRNA levels of Sod1, Opa1, Mfn2, Drp1, Sirt1 and Sirt3 in oocytes.ResultsThe NMN supplementation increased the oocyte maturation rate of the mice with diabetes. Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.ConclusionNMN could improve the maturation rate and quality of oocytes in STZ-induced diabetic mice, which provides a significant clue for the treatment of infertility of the patients with diabetes.

Correlations between Glycated Hemoglobin (HbA1c) and Oral Glucose Tolerance test, OGTT (and Some Cardiovascular Risk Factors) in a Cohort Population of Patients at the University of Port Harcourt Teaching Hospital (UPTH), Niger Delta Region of Nigeria

Background: Diabetes mellitus (DM) is increasing in prevalence globally and in Nigeria today. Fasting blood glucose and oral glucose tolerance test (OGTT) have been the traditional gold standard tests for diagnosis of DM until recently when glycated hemoglobin (HbA1c) is being used. Is there a correlation between the two? Objectives: The aim of this study was to determine the level of correlation between HbA1c and OGTT and some cardiovascular risk factors in a certain population in UPTH, Nigeria. Materials and Methods: This was a cross-sectional study involving 400 consecutively attending patients at the medical and general outpatient clinics of UPTH. Results: The HbA1C had a strong positive correlation with the 2 h post-glucose load of the OGTT (r = 0.652, P &lt; 0.001). The HbA1c also had a positive correlation with the body mass index, waist circumference, age, total cholesterol (TC), triglyceride levels, and low-density lipoprotein cholesterol levels, but it was most positively correlated with the TC (r = 0.582, P &lt; 0.001). It was, however, negatively correlated with the high-density lipoprotein cholesterol (r = −0.166, P = 0.001).Conclusion: Glycated HbA1c had a positive correlation with OGTT, and most of the cardiovascular risk factors of DM studied.

Application of Clinical Blood Metabogram to Type 2 Diabetes Mellitus.

The clinical blood metabogram (CBM) was developed to match a tailored analysis of the blood metabolome to the time, cost, and reproducibility constraints of clinical laboratory testing. By analyzing the main blood metabolite groups, CBM offers clinically relevant information about the intake of low-molecular substances into the organism, humoral regulation, liver function, amino acid level, and the lipid and carbohydrate metabolism. The purpose of this work was to investigate the relevance of using the CBM in patients with diabetes mellitus. For this, a CBM was obtained for 18 healthy individuals, 12 individuals with prediabetes, and 64 individuals with type 2 diabetes mellitus, separated into groups according to fasting blood glucose and oral glucose tolerance tests. The results showed that the CBM reveals diabetes-associated metabolic alterations in the blood, including changes in the levels of carbohydrates, ketone bodies, eicosanoids, phospholipids, and amino acids, which are consistent with the scientific data available to date. The CBM enabled the separation of diabetic patients according to their metabolic metabotypes, providing both a general overview of their metabolic alterations and detailing their individual metabolic characteristics. It was concluded that the CBM is a precise and clinically applicable test for assessing an individual's metabolic status in diabetes mellitus for diagnostic and treatment purposes.

Role of Pathology and Point of Care Testing in the Estimation of Fasting Blood Glucose Levels in Type 2 Diabetes Mellitus Patients

Background: Diabetes mellitus (DM) is a global health concern with significant morbidity and mortality. Effective management of type 2 DM necessitates accurate monitoring of fasting blood glucose levels. Traditional laboratory methods, while accurate, are not always convenient for patients, leading to a growing interest in reliable point-of-care testing. Objective: The aim of this study was to compare the accuracy of fasting blood glucose measurements between point-of-care testing using a glucometer and traditional laboratory methods (hexokinase and end-point) to determine the feasibility of home-based patient monitoring. Methods: This comparative cross-sectional study included 150 type 2 DM patients from Farooq Hospital Westwood, Lahore. Participants underwent fasting blood glucose testing using three methods: the hexokinase method, the end-point method, and a glucometer (Accu-check instant S Meter). The Pearson correlation was employed to assess the linear relationship between the methods, while multivariate ANOVA was used to compare the means. Results: The Pearson correlation coefficients indicated a strong positive correlation between the glucometer readings and both laboratory methods, with coefficients of 0.994 for hexokinase and 0.997 for the end-point method. The average fasting blood glucose levels measured were 128.43 mg/dL (±61.257) for hexokinase, 128.59 mg/dL (±61.110) for the end-point method, and slightly lower at 123.59 mg/dL (±58.920) for the glucometer. Conclusion: The study concluded that glucometer readings are highly correlated with traditional laboratory methods, indicating that point-of-care testing can be a reliable alternative for patients to monitor fasting blood glucose levels at home.

A comparative study of the glucose challenge test, fasting blood glucose test, and post-prandial blood glucose screening to identify gestational diabetes mellitus

A comparative study of the glucose challenge test, fasting blood glucose test, and post-prandial blood glucose screening to identify gestational diabetes mellitus