Objective. The goal of this study was to perform real-time estimation of isometric finger extension force using the discharge information of motor units (MUs). Approach. A real-time electromyogram (EMG) decomposition method based on the fast independent component analysis (FastICA) algorithm was developed to extract MU discharge events from high-density (HD) EMG recordings. The decomposition was first performed offline during an initialization period, and the obtained separation matrix was then applied to new data samples in real-time. Since MU pool discharge probability reflects the neural drive to spinal motoneurons, individual finger forces were estimated based on a firing rate-force model established during the initialization, termed the neural-drive method. The conventional EMG amplitude-based method was used to estimate the forces as a comparison, termed the EMG-amplitude method. Simulated HD-EMG signals were first used to evaluate the accuracy of the real-time decomposition. Experimental EMG recordings of 5 min of isometric finger extension with pseudorandom force levels were used to assess the performance of force estimation over time. Main results. The simulation results showed that the accuracy of real-time decomposition was 86%, compared with an offline accuracy of 94%. However, the real-time decomposition accuracy was stable over time. The experimental results showed that the neural-drive method had a significantly smaller root mean square error (RMSE) of the force estimation compared with the EMG-amplitude method, which was consistent across fingers. Additionally, the RMSE of the neural-drive method was stable until 230 s, while the RMSE of the EMG-amplitude method increased progressively over time. Significance. The neural-drive method on real-time finger force estimation was more accurate over time compared with the conventional EMG-amplitude method during prolonged muscle contractions. The outcomes can potentially offer a more accurate and robust neural interface technique for reliable neural-machine interactions based on MU pool discharge information.