In recent years, with the continuous growth of China’s power peak load and the rapid development of renewable energy, a large number of renewable energy sources are connected to the power grid, increasing the uncertainty of power grid operation and posing new major challenges to the power system regulation capacity. Flexible load has the characteristics of wide distribution, fast response, and high economy, which is an important control resource for the future power system. Based on the flexible load of commercial buildings and residential users, this paper studies the resource characteristics and response characteristics, clarifies the resource characteristics and demand response characteristic indexes of commercial and residential users, and establishes the response characteristics model of commercial buildings and residential users. Considering the influence of weather, holidays, incentive mechanism, and other factors on the response of flexible load, the quantitative analysis method of flexible load resource regulation potential for regional power grid dispatching was studied, and the feasibility of flexible load resources directly participating in the load control system was analyzed. Based on the uncertainty and mathematical characterization method of the active response of flexible loads, the optimal combination control strategy of demand response resources was proposed to eliminate the problems of heavy load and overload of regional power grid equipment by using the active response ability of flexible loads. Finally, the IEEE 14-node system is selected for simulation verification, which provides a theoretical basis for alleviating the power grid operation pressure in the peak load period of the power grid in the urban core area, improving the safety and economic operation level of regional power grid dispatching and the utilization rate of power grid equipment assets.