Fast Readout Research Articles

QCMOS Detectors and the Case of Hypothetical Primordial Black Holes in the Solar System, near Earth Objects, Transients, and Other High-cadence Observations

Abstract Recent progress with CMOS detector development has opened new parameter space for high cadence time resolved imaging of transients and fast proper motion solar system objects. Using computer simulations for a ground-based 1.23 m telescope, this research note illustrates the gain of a new generation of fast readout low noise qCMOS sensors over CCDs and makes the case for high precision monitoring of asteroid orbits that can potentially shed light on the hypothetical existence of low mass primordial black holes, as well as for other applications requiring high speed imaging.

Ultrafast superconducting qubit readout with the quarton coupler.

Fast, high-fidelity, and quantum nondemolition (QND) qubit readout is an essential element of quantum information processing. For superconducting qubits, state-of-the-art readout is based on a dispersive cross-Kerr coupling between a qubit and its readout resonator. The resulting readout can be high fidelity and QND, but readout times are currently limited to the order of 50 nanoseconds due to the dispersive cross-Kerr of magnitude 10 megahertz. Here, we present a readout scheme that uses the quarton coupler to facilitate a large (greater than 200 megahertz) cross-Kerr between a transmon qubit and its readout resonator. Full master equation simulations of the coupled system show a 5-nanosecond readout time with greater than 99% readout fidelity and greater than 99.9% QND fidelity. The quartonic readout circuit is experimentally feasible and preserves the coherence properties of the qubit. Our work reveals a path for order of magnitude improvements of superconducting qubit readout by engineering nonlinear light-matter couplings in parameter regimes unreachable by existing designs.

Magnetic microbead-based upconversion immunoassay with laser-induced breakdown spectroscopy readout for the detection of prostate-specific antigen.

Laser-induced breakdown spectroscopy (LIBS) is a promising technique for the readout of immunochemical assays utilizing indirect detection of labels (Tag-LIBS), typically based on nanoparticles. We have previously demonstrated that Tag-LIBS immunoassay employing yttrium-based photon-upconversion nanoparticles (UCNPs) can reach sensitivity similar to commonly used enzyme and fluorescence immunoassays. In this study, we report on further increasing the sensitivity of UCNP-based Tag-LIBS immunoassay by employing magnetic microbeads (MBs) as the solid phase in the determinationof cancer biomarker prostate-specific antigen. Due to the possibility of analyte preconcentration, MBs enabled achieving a limit of detection (LOD) of 4.0pg·mL-1, representing two orders of magnitude improvement compared withequivalent microtiter plate-based assay (LOD of 460pg·mL-1). In addition, utilizing MBs opens up the possibility of an internal standardization of the LIBS readout by employing iron spectral lines, which improves the assay robustness by compensating for LIBS signal fluctuations and bead-bound immunocomplexes lost throughout the washing steps. Finally, the practical applicability of the technique was confirmed by the successful analysis of clinical samples, showing a strong correlation with the standard electrochemiluminescence immunoassay. Overall, MB-based Tag-LIBS was confirmed as a promising immunoassay approach, combining fast readout, multiplexing possibilities, and high sensitivity approaching upconversion luminescence scanning while avoiding the requirement of luminescence properties of labels.

Large-Angle Rocking Beam Electron Diffraction of Large Unit Cell Crystals Using Direct Electron Detector.

We report a large-angle rocking beam electron diffraction (LARBED) technique for electron diffraction analysis. Diffraction patterns are recorded in a scanning transmission electron microscope (STEM) using a direct electron detector with large dynamical range and fast readout. We use a nanobeam for diffraction and perform the beam double rocking by synchronizing the detector with the STEM scan coils for the recording. Using this approach, large-angle convergent beam electron diffraction (LACBED) patterns of different reflections are obtained simultaneously. By using a nanobeam, instead of a focused beam, the LARBED technique can be applied to beam-sensitive crystals as well as crystals with large unit cells. This paper describes the implementation of LARBED and evaluates the performance using silicon and gadolinium gallium garnet crystals as test samples. We demonstrate that our method provides an effective and robust way for recording LARBED patterns and paves the way for quantitative electron diffraction of large unit cell and beam-sensitive crystals.

Enhance optically stimulated luminescence sensitivity and improve fading of newly developed LiMgPO4:Er phosphor

To avoid fading, the readout time of the old LiMgPO4:Er in the previous study had to be taken to be at least 20 s. The readout time is up to 20 s, which not only contradicts the fast OSL readout, but also eliminates too many OSL signals per readout and is not favourable to the requirement that the OSL can be read multiple times. The primary objective of this study is to reduce readout time under conditions where the OSL signal of LiMgPO4:Er phosphor stabilizes after irradiation. The OSL properties were investigated. To achieve OSL fading of about 5% within 30 d after irradiation, the shortest integration time for new LiMgPO4:Er in this study was 1 s, whereas old LiMgPO4:Er required 20 s. When the integration time was 1 s, the OSL sensitivity of the new LiMgPO4:Er samples in this study was about 2.4 times that of the old LiMgPO4:Er samples. A new LiMgPO4:Er phosphor with an OSL signal loss of about 5% for the shortest integration time of 1 s within 30 days after irradiation, a sensitivity comparable to that of TLD-500k, and an MMD as low as 38 μGy was successfully prepared, which will meet the fast read out and the multiple readings requirements.

High-transmission spectrometer for rapid resonant inelastic soft X-ray scattering (rRIXS) maps.

The design and first results of a high-transmission soft X-ray spectrometer operated at the X-SPEC double-undulator beamline of the KIT Light Source are presented. As a unique feature, particular emphasis was placed on optimizing the spectrometer transmission by maximizing the solid angle and the efficiencies of spectrometer gratings and detector. A CMOS detector, optimized for soft X-rays, allows for quantum efficiencies of 90% or above over the full energy range of the spectrometer, while simultaneously offering short readout times. Combining an optimized control system at the X-SPEC beamline with continuous energy scans (as opposed to step scans), the high transmission of the spectrometer, and the fast readout of the CMOS camera, enable the collection of entire rapid resonant inelastic soft X-ray scattering maps in less than 1 min. Series of spectra at a fixed energy can be taken with a frequency of up to 5 Hz. Furthermore, the use of higher-order reflections allows a very wide energy range (45 to 2000 eV) to be covered with only two blazed gratings, while keeping the efficiency high and the resolving power E/ΔE above 1500 and 3000 with low- and high-energy gratings, respectively.

Fast readout of identity-related information in multiple images after incidental learning

Fast readout of identity-related information in multiple images after incidental learning

CRISPR-enabled point-of-care genotyping for APOL1 genetic risk assessment

Detecting genetic variants enables risk factor identification, disease screening, and initiation of preventative therapeutics. However, current methods, relying on hybridization or sequencing, are unsuitable for point-of-care settings. In contrast, CRISPR-based-diagnostics offer high sensitivity and specificity for point-of-care applications. While these methods have predominantly been used for pathogen sensing, their utilization for genotyping is limited. Here, we report a multiplexed CRISPR-based genotyping assay using LwaCas13a, PsmCas13b, and LbaCas12a, enabling the simultaneous detection of six genotypes. We applied this assay to identify genetic variants in the APOL1 gene prevalent among African Americans, which are associated with an 8–30-fold increase in the risk of developing kidney disease. Machine learning facilitated robust analysis across a multicenter clinical cohort of more than 100 patients, accurately identifying their genotypes. In addition, we optimized the readout using a multi-analyte lateral-flow assay demonstrating the ability for simplified genotype determination of clinical samples. Our CRISPR-based genotyping assay enables cost-effective point-of-care genetic variant detection due to its simplicity, versatility, and fast readout.

Spiral 3DREAM sequence for fast whole-brain B1 mapping.

This work demonstrates a new variant of the 3DREAM sequence for whole-brain mapping employing a three-dimensional (3D) stack-of-spirals readout. The spiral readout reduces the echo train length after the STEAM preparation in order to overcome the significant blurring in STE* images due to the decreasing STE* signal with each excitation pulse. The 3DREAM sequence rapidly acquires two contrasts to calculate whole-brain flip angle maps. In the proposed spiral 3DREAM sequence, the Cartesian readout scheme is replaced by an accelerated 3D stack-of-spirals readout with a CAIPIRINHA sampling scheme. Phantom experiments were conducted to compare flip angle maps of the spiral 3DREAM sequence to a Cartesian 3DREAM sequence, an actual flip-angle-imaging (AFI) sequence, the dual-angle method, and the Bloch-Siegert shift method. Afterwards, the results were validated in vivo acquiring flip angle maps from five subjects. Flip angle maps of the spiral 3DREAM sequences showed high agreement with the reference methods both in phantom and in vivo experiments. Blurring in STE* images and flip angle maps was reduced compared to the Cartesian 3DREAM sequence. The spiral 3DREAM sequence utilizes a fast readout minimizing the echo train length of the imaging train. This reduces blurring in STE* images as well as the total acquisition time and increases the effective resolution of maps.

Characterisation studies of two front-end electronics chips designed for SiPM readout

A new front-end ASIC named ”PIST” (pico-second timing) has been successfully developed using 55 nm CMOS technology for the silicon photomulplier (SiPM) readout with a single channel with a major aim of fast timing. We performed extensive tests to evaluate the timing performance of a dedicated test stand equipped with a PIST chip. The results show that the system timing resolution can reach sub 10 ps for large SiPM signals, while the PIST intrinsic timing resolution is better than 5 ps. The PIST dynamic range has been further extended using the time-over-threshold (ToT) technique. Meanwhile, we fully characterised a new commercially available SiPM-readout 32-channel ASIC for developments of future high-granularity crystal calorimetry, including the single photon calibration and the dynamic range of different gain regions. Other promising potentials include fast timing resolution, fast readout speed and low power dissipation. Comprehensive measurements were made with a laser beam and high-energy particle beams with crystals and SiPMs. First testing results show that this chip has an excellent signal-to-noise and a large dynamic range. This contribution will introduce the ASICs as well as dedicated test stands and also present highlighted results including the timing resolution, single photon calibration and dynamic range.

Flip-Chip-Based Fast Inductive Parity Readout of a Planar Superconducting Island

The properties of superconducting devices depend sensitively on the parity (even or odd) of the quasiparticles that they contain. Encoding quantum information in the parity degree of freedom is central in several emerging solid-state qubit architectures, including in hybrid superconductor-semiconductor devices. In the latter case, accurate, nondestructive, and time-resolved parity measurements are a challenging issue. Here, we report on control and real-time parity measurement in a superconducting island embedded in a superconducting loop and realized in a hybrid two-dimensional heterostructure using a microwave resonator. To avoid microwave losses impeding time-resolved measurements, the device and readout resonator are located on separate chips, connected via flip-chip bonding, and couple inductively through vacuum. The superconducting resonator detects the parity-dependent circuit inductance, allowing for fast parity readout. We have resolved even- and odd-parity states with a signal-to-noise ratio of SNR≈3 for an integration time of 20μs and a detection fidelity exceeding 98%. The real-time parity measurement shows a state lifetime extending into the millisecond range. Our approach will lead to a better understanding of coherence-limiting mechanisms in superconducting quantum hardware and help to advance inductive-readout schemes for hybrid qubits. Published by the American Physical Society 2024

Readout circuit for a ZnO bulk-acoustic-wave X-ray dose rate detector

Bulk Acoustic Wave (BAW) resonators based on piezoelectric semiconductors are radio frequency devices that can convert X-ray dose rate into resonant frequency offset, which exhibit strong anti-interference capabilities and low power consumption and are suitable for wireless sensing systems. In this study, we designed a readout circuit for a ZnO BAW X-ray dose rate detector. (0001)-oriented ZnO single crystals were used to fabricate the high-quality BAW X-ray detector. Under dark field and X-ray irradiation conditions, the resonant frequency offset of the detector was recorded via the readout circuit. The readout circuit consists of an oscillator circuit based on a Pierce oscillator circuit structure, a frequency mixing circuit, and an STM32 microcontroller. This design offers higher frequency stability and lower phase noise. In practical applications, it can replace the expensive and bulky vector network analyzer for detecting and storing the resonant frequency of BAW resonators with the advantages of fast readout speed and portability.

Quantum paraelectric varactors for radiofrequency measurements at millikelvin temperatures

Radiofrequency reflectometry can provide fast and sensitive electrical read-out of charge and spin qubits in quantum dot devices coupled to resonant circuits. In situ frequency tuning and impedance matching of the resonator circuit using voltage-tunable capacitors (varactors) is needed to optimize read-out sensitivity, but the performance of conventional semiconductor- and ferroelectric-based varactors degrades substantially in the millikelvin temperature range relevant for solid-state quantum devices. Here we show that strontium titanate and potassium tantalate, materials which can exhibit quantum paraelectric behaviour with large field-tunable permittivity at low temperatures, can be used to make varactors with perfect impedance matching and resonator frequency tuning at 6 mK. We characterize the varactors at 6 mK in terms of their capacitance tunability, dissipative losses and magnetic field insensitivity. We use the quantum paraelectric varactors to optimize the radiofrequency read-out of carbon nanotube quantum dot devices, achieving a charge sensitivity of 4.8 μe Hz−1/2 and a capacitance sensitivity of 0.04 aF Hz−1/2.

Characterization of Tracking Modules Based on DSSD Sensors at the SC-1000 Accelerator for the BM@N Project

The results of studying characteristics of modules based on double-sided microstrip silicon sensors, which are designed to create the wide-aperture Silicon Tracking System for the BM@N facility, are presented. The main module features are the use of fast readout electronics based on the STS-XYTER chip and the application of ultralight (0.23% X0) aluminum microcables for transmitting analog signals from the sensor strips to the input channels of the readout electronics. The results of testing different configurations of tracking modules on the extracted beam of protons at the SC-1000 accelerator in the PNPI are presented. The stable operation of readout electronics is demonstrated at loads close to the maximum values of 360 kHz s–1 cm–2. The signal-to-noise ratio was no less than 23. The measured coordinate resolution of the modules within the beam telescope was 17 ± 0.4 μm, and the detector efficiency of recording for protons with energy of 1 GeV was no less than 99%.

Point-of-Care Testing of Whole Blood for Liver Injury Auxiliary Diagnosis with Biothiols Activable Chemiluminescent Probe.

Liver injury significantly affects a patient's health and quality of life. However, timely and convenient diagnosis of this disease via whole blood detection remains challenging due to the lack of user-friendly and fast readout blood test methods. Herein, we developed such a method for the swift auxiliary diagnosis of liver injury via whole blood detection using a customed point-of-care testing (POCT) system consisting of a biothiols-activatable chemiluminescent probe and a hand-held POCT device. Biothiols served as the target to build the activable chemiluminescence probe due to their abnormal level in liver injury. Compared with fluorescent and electrical POCTs, this method is more convenient and has strong universality. By incorporating cyclodextrin via host-guest chemistry, we intensified chemiluminescence while mitigating chemical hemolysis caused by the dissolution of organic molecules, making this system suitable for whole blood analysis. Preliminary assessments in aqueous solutions, living cells, and mouse models confirmed its sensitivity, reliability, and feasibility. Simply mixing blood with the probe for 30 min yielded a clear signal readout within 15 s on the POCT device. Utilizing this portable detector, the reduced biothiol level was tested in 18 liver injury patient blood samples, and the results were similar to those measured by a commercial kit and in vivo imaging system. Thus, this work provides a universal platform for the fast and convenient detection of other biomarkers in whole blood samples and opens up possibilities for the rapid clinical diagnosis of diseases, enabling patients to conduct home self-examinations with ease.

Tuning Exciton Emission via Ferroelectric Polarization at a Heterogeneous Interface between a Monolayer Transition Metal Dichalcogenide and a Perovskite Oxide Membrane.

We demonstrate the integration of a thin BaTiO3 (BTO) membrane with monolayer MoSe2 in a dual-gate device that enables in situ manipulation of the BTO ferroelectric polarization with a voltage pulse. While two-dimensional (2D) transition metal dichalcogenides (TMDs) offer remarkable adaptability, their hybrid integration with other families of functional materials beyond the realm of 2D materials has been challenging. Released functional oxide membranes offer a solution for 2D/3D integration via stacking. 2D TMD excitons can serve as a local probe of the ferroelectric polarization in BTO at a heterogeneous interface. Using photoluminescence (PL) of MoSe2 excitons to optically read out the doping level, we find that the relative population of charge carriers in MoSe2 depends sensitively on the ferroelectric polarization. This finding points to a promising avenue for future-generation versatile sensing devices with high sensitivity, fast readout, and diverse applicability for advanced signal processing.

Ultrasensitive graphene field-effect transistor biosensor for rapidly detecting miRNA-208a

MicroRNA-208a (miR208a) is a cardiac-specific miRNA and can provide an earlier prediction for AMI onset than currently used cardiac biomarkers. Graphene field-effect transistor (GFET) biosensor, as the promising candidate for next-generation biosensor, has been demonstrated for real-time detection of a variety of bioactive molecules with ultrasensitivity from nM to fM level. Currently, research on GFET sensors mostly focuses on the properties of the devices themselves, rather than aiming for ultra-sensitive detection based on biochemical substances. In this study, we developed a liquid-gated graphene field-effect transistor-based biosensor for detection of miR208a and designed a portable device to adapt with it for a fast readout of the electronic response, achieving a detection range of 0.01–1 pM, detection limit of 5.3 fM and detection time of 40 minutes. Our work provides a reliable strategy to rapidly and ultrasensitively quantifying miRNA-208a, which also holds great potential to be extended in a wide range of applications of other disease markers and lead to powerful tools for healthcare.

Intrinsic noise of the single-electron box

The radio-frequency (rf) single-electron box is becoming an attractive charge sensor for semiconductor-based quantum computing devices due to its high sensitivity and small footprint, which facilitate the design of highly connected qubit architectures. However, an understanding of its ultimate sensitivity is missing due to the lack of a noise model. Here, we quantify the intrinsic noise of the single-electron box arising from stochastic cyclic electron tunneling between a quantum dot and a reservoir driven by a periodic gate voltage. We use both a master-equation formalism and Markov Monte Carlo simulations to calculate the gate-noise current and find that the noise mechanism can be represented as a cyclostationary process. We consider the implications of this cyclostationary noise on the ultimate sensitivity of single-electron box sensors for fast high-fidelity readout of spin qubits, in particular evaluating results for rf reflectometry implementations and the back action of the sensor on a qubit. Furthermore, we determine the conditions under which the intrinsic noise limit could be measured experimentally and techniques by which the noise can be suppressed to enhance qubit-readout fidelity. Published by the American Physical Society 2024

Real-time structural characterization of protein response to a caged compound by fast detector readout and high-brilliance synchrotron radiation

Protein dynamics are essential to biological function, and methods to determine such structural rearrangements constitute a frontier in structural biology. Synchrotron radiation can track real-time protein dynamics, but accessibility to dedicated high-flux single X-ray pulse time-resolved beamlines is scarce and protein targets amendable to such characterization are limited. These limitations can be alleviated by triggering the reaction by laser-induced activation of a caged compound and probing the structural dynamics by fast-readout detectors. In this work, we established time-resolved X-ray solution scattering (TR-XSS) at the CoSAXS beamline at the MAX IV Laboratory synchrotron. Laser-induced activation of caged ATP initiated phosphoryl transfer in the adenylate kinase (AdK) enzyme, and the reaction was monitored up to 50ms with a 2-ms temporal resolution achieved by the detector readout. The time-resolved structural signal of the protein showed minimal radiation damage effects and excellent agreement to data collected by a single X-ray pulse approach.

In planta imaging of pyridine nucleotides using second-generation fluorescent protein biosensors.

Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent biosensors are considered to be the best option to bridge the existing technology gap, as they provide a fast, accurate, and real-time readout. However, the existing pyridine nucleotides genetically encoded fluorescent biosensors are either sensitive to pH change or slow in dissociation rate. Herein, we employed the biosensors which generate readouts that are pH stable for in planta measurement of NADH/NAD+ ratio and NADPH level. We generated transgenic Arabidopsis lines that express these biosensors in plastid stroma and cytosol of whole plants and pollen tubes under the control of CaMV 35S and LAT52 promoters, respectively. These transgenic biosensor lines allow us to monitor real-time dynamic changes in NADH/NAD+ ratio and NADPH level in the plastids and cytosol of various plant tissues, including pollen tubes, root hairs, and mesophyll cells, using a variety of fluorescent instruments. We anticipate that these valuable transgenic lines may allow improvements in plant redox biology studies.