ABSTRACT OpenCourseWare (OCW) and Massive Open Online Courses (MOOCs) have gained increasing popularity and importance because they greatly expand students’ access to university lectures. Although the main component of OCW and MOOC platforms is lectures, their differences may potentially affect the difficulty of academic listening comprehension. This study thus explores the extent to which the differences between OCW and MOOC lectures may affect L2 academic listening comprehension in terms of three affecting factors: academic vocabulary coverage, lexical density, and speech rate. The result shows that both OCW and MOOC lectures have a high academic vocabulary coverage, with many more higher-level academic words appearing in the current MOOC lectures. Moreover, the lexical density and speech rate of MOOC lectures are significantly higher than those of OCW lectures, suggesting that students may need to process denser information delivered at a faster speech rate when viewing MOOC lectures. It is thus concluded that comprehending MOOC lectures may demand higher-level academic listening skills and a larger academic vocabulary size. The findings can provide implications for EAP instruction in terms of using OCW and MOOC lectures as academic listening materials for students with different academic vocabulary knowledge and listening proficiency levels.