Abstract According to their respective positions, participants at the local, regional, and global actors within the poultry farming sector play strategic roles according to their respective positions. While these roles may integrate and overlap, they can also generate conflicts of interest at both the actor and institutional levels. This study analyses the actors and power relations in laying hen farming in Blitar Regency, East Java, Indonesia. The data were collected using structured interview techniques (interview guides and Matrix of Alliances and Conflicts: Tactics, Objectives and Recommendations questionnaires), observation, documentation, and focus group discussions. The research informants were chosen deliberately based on various sources including farmers, farmer groups, cooperative, central and local government, companies, and traders. This was in addition to informants obtained using the snowball sampling technique such as middlemen, poultry shops (PSs) and other additional breeders. Data validity was determined using source triangulation and method triangulation. The results revealed that the individuals and entities engaged in layer hen farming encompass a diverse range of actors. These include farmers of various scales (small, medium, and large), institutions such as farmer groups and cooperatives, governmental bodies at both central and regional levels, industry representatives such as companies, PSs, distributors involved in producing and marketing day-old chick (DOC), feed, medicines, and vaccines, as well as market participants including local traders and traders operating across different regions. Small-scale and medium-scale farmers have the strongest relationships due to their high dependence on inputs such as DOC, feed, vaccines, and access to markets. The presence of various actors and power relations in laying hen business institutions creates both collaboration and competition in the supply of DOCs, animal feed, medicine, and even in the marketing of eggs from a food security and sustainability perspective.