Fabric made from fancy yarn, especially neppy yarn has a great environmental impact because the fabric does not need any additional dyeing process. This study involved knitting neppy yarn of two distinct spinning processes, vortex and ring spinning, each having three different percentages of neps. The comfort properties of six different fabrics were evaluated by analyzing various parameters, including moisture management properties, water vapor transmission rate, air permeability, hand feel properties, and bursting strength. Microscopic views were also examined for analyzing the physical appearance of the fabric’s surface. The experimental results revealed that fabrics made from vortex-spun neppy yarn (VSNY) exhibit better moisture management, breathability, and hand feel properties than ring-spun neppy yarn (RSNY). Alternatively, bursting strength and water vapor evaporation rate showed a lower trend in fabrics made from vortex-spun neppy yarn. The research findings contribute valuable insights into the potential of vortex spinning techniques for sustainable and comfortable textile production.