Work-family conflict, an important issue in nursing management, has been widely studied around the world. Over the past 20 years, many hospitals and care institutions have been faced with an increasing demand for healthcare due to an ageing population. The purpose of this study is to to determine the prevalence and factors Associate with the occurrence of WFC among nurses in the town of Mbujimayi. A cross-sectional study was conducted in 9 referral hospitals in the town of Mbuji-Mayi. Data were collected using a questionnaire, including items related to nurses’ demographic characteristics, the WFC questionnaire. Our study indicates that the prevalence of work-family conflict among nurses was 49.8%. Nurses’ age [ORa: 12.13(1.54-2.93)], marital status [ORa: 1.54(1.15-2.07)], hospital categories[ORa:1.59(1.15-2.21)], work experience in years [ORa: 3.20(2.16-4.75)], occupancy status[ORa: 1.38(1.03-1.85)] and having an additional activity[ORa: 1.38[1.03-1.85]) predicted work–family conflict. Given the extent of work-life conflict among nurses and its negative repercussions on both their health and the quality of their care, preventive measures aimed at improving the work-life balance should be introduced in the care environment.