Human fatalities and injury from wildlife attacks often result in a negative attitude toward conservation. This research was undertaken to investigate the patterns and conflict-causing factors of human killing and injury by large mammals, especially by Asian elephant (Elephas maximus), common leopard (Panthera pardus), and Bengal tiger (Panthera tigris tigris) in the Bardia-Banke Complex of western Nepal. We collected human death and injury records caused by wildlife in the Bardia-Banke Complex between 2019 and 2023, based on relief applications submitted by the victim's family. Additionally, camera trap monitoring was conducted following incidents of human-tiger and human-leopard conflicts. A total of 76 incidents involving human casualties and injuries were considered for analysis. Incidents of livestock depredation, crop raiding, and property damage were excluded from the analysis. Most of the attacks on humans were caused by tigers (75%), followed by elephants (16%) and leopards (9%). Almost all incidents occurred in daytime (97%). The highest number of conflicts were recorded in 2021, with 20 incidents. Most of the cases (84%) occurred within 1 km of forest edge. Khata corridor and the western side of the Bardia National Park, i.e., Karnali River corridor, were identified as high-conflict areas. The primary causes of the conflict manifested in cattle grazing (28%), grass cutting (28%), firewood collection (11%), fishing (8%), vegetable collection (5%), sand collection (4%), during rescuing friends (3%), grazing captive elephants (3%), highway rides (3%), sleeping in Chaupadi Goth (3%), walking nearby forest areas (3%), playing nearby forest areas (1%), while feeding pig (1%), and working in agricultural lands (1%). To promote human-wildlife coexistence, community-based patrols (33%), habitat restoration (26%), electric fencing (26%), and insurance (7%) were identified as the preferred strategies. Therefore, we recommend that stakeholders and concerned bodies increase awareness among local community about the use of forest resources, wildlife behavior, and human-wildlife conflict mitigation strategies.