Family Caryophyllaceae Research Articles

Morphological, cytogenetic, and phytochemical estimation of Lychnis flos-cuculi L. cell cultures: can cell biomass be a source of bioactive compounds?

The ragged robin (Lychnis flos-cuculi L.) from the Caryophyllaceae family contains flavonoids, phenolic acids, flavonoids, triterpenoid saponins, and ecdysteroids, making it potential for biological and medicinal application. Plant cell culture technologies could be beneficial for obtaining low-cost biomass sources with valuable phytochemicals sustainably. The current study reports the induction and proliferation of ragged robin cell culture and evaluation of various plant growth regulators (PGRs) impact on the secondary metabolites profile using UPLC-HRMS/MS. Callus was initiated from different explants on the Murashige and Skoog medium with various combinations and concentrations of PGRs. It was found that prolonged proliferation on MS medium enriched with a combination of 1.0 mg l− 1 2,4-Dichlorophenoxyacetic acid and 0.1 mg l− 1 N6-furfuryl adenine (Kinetin) yields the maximum biomass with 504.05% growth index. The results of flow cytometric analysis suggested no genetic alteration between differentiated and dedifferentiated cultures. Furthermore, the UPLC-HRMS/MS result distinguished 101 significant signals that were manually identified as ecdysteroids (16 metabolites), flavonoids (11 metabolites), phenolic acids (10 metabolites), triterpene saponins and sapogenins (53 metabolites), and some primary metabolites. Our study demonstrates that the cell culture of L. flos-cuculi displays significant differences in its phytochemical makeup to plants cultivated in vitro. Moreover, the type of growth regulators used for callus cultures was identified to cause substantial shifts in the identified metabolites.Graphical

In Vitro COX Inhibitory Activity, LC-MS Analysis and Molecular Docking Study of Silene vulgaris and Stellaria media.

Silene vulgaris (Moench) Garcke and Stellaria media (L.) Vill is a perennial wild weed species belonging to the Caryophyllaceae family and is widely available and abundant in the environment. The present study has aimed to evaluate the anti-inflammatory potential of two underutilized wild edible plants, Silene vulgaris (Moench) Garcke and Stellaria media (L.) Vill. fractions employing in-vitro COX inhibitory assay. Invitro COX-2 inhibitory potential of MESV and MESM fractions was carried out using BioVisionR "COX Activity Assay Kit (Fluorometric)". LC-MS analysis of selected fractions was conducted to identify bioactive compounds that were further validated for their affinity determination toward target enzymes employing molecular docking studies using the LibDock program. In-vitro COX inhibitory assay revealed that hexane fraction of S. vulgaris (HFSV) and hexane fraction of S. media (HFSM) caused impressive inhibition of COX-2 enzyme with IC50 values 1.38 µg/mL and 1.51 µg/mL respectively. Further, LC-MS analysis revealed the presence of 46 compounds in HFSV and 44 compounds in HFSM respectively. Amongst identified bioactive compounds in HFSV and HFSM, sinapinic acid and syringic acid showed good docking scores with COX-2 i.e., 89.256, and 82.168 respectively. Also, the availability of chrysin in HFSM and rhamnetin in HFSV exhibited good docking scores i.e., 115.092, and 112.341 with a selective affinity towards COX-2. The findings of in-vitro COX Inhibitory Activity and molecular docking studies highlighted the impressive anti-inflammatory properties of S. vulgaris and S. media, and require further investigations to establish them as therapeutic candidates in the management of inflammation and related issues.

Oligosaccharides from Stellaria dichotoma L. var. lanceolate bind to galectin-3 and ameliorate effects of colitis

Even though Stellaria dichotoma L. var. lanceolate (S. dichotoma) is a well-known medicinal plant in the family Caryophyllaceae, its oligosaccharides remain unexplored in terms of their potential as bioactive agents. Here, we isolated a mixture of oligosaccharides from S. dichotoma (Yield: 12 % w/w), that are primarily non-classical raffinose family oligosaccharides (RFOs). Nine major oligosaccharides were purified and identified from the mixture, including sucrose, raffinose, 1-planteose, lychnose, stellariose, along with four new non-classical RFOs. Two of the four new oligosaccharides are linear hexose pentamers with α-galactosyl extensions on their lychnose moieties, and the other two are branched hexose hexamers with α-galactosyl extensions on their stellariose groups. Their interactions with galectin-3 (Gal-3) revealed significant binding, with the terminal galactose providing enhanced affinity for the lectin. Notably, Gal-3 residues Arg144, His158, Asn160, Arg162, Asn174, Trp181, Glu184 and Arg186 coordinate with the lychnose. In vivo studies using the dextran sulfate sodium (DSS) mouse model for colitis demonstrated the ability of these carbohydrates in mitigating ulcerative colitis (UC). Overall, our study has provided structural information and potential applications of S. dichotoma oligosaccharides, also offers new approaches for the development of medicinal oligosaccharides.

Unlocking saponin biosynthesis in soapwort.

Soapwort (Saponaria officinalis) is a flowering plant from the Caryophyllaceae family with a long history of human use as a traditional source of soap. Its detergent properties are because of the production of polar compounds (saponins), of which the oleanane-based triterpenoid saponins, saponariosides A and B, are the major components. Soapwort saponins have anticancer properties and are also of interest as endosomal escape enhancers for targeted tumor therapies. Intriguingly, these saponins share common structural features with the vaccine adjuvant QS-21 and, thus, represent a potential alternative supply of saponin adjuvant precursors. Here, we sequence the S. officinalis genome and, through genome mining and combinatorial expression, identify 14 enzymes that complete the biosynthetic pathway to saponarioside B. These enzymes include a noncanonical cytosolic GH1 (glycoside hydrolase family 1) transglycosidase required for the addition of D-quinovose. Our results open avenues for accessing and engineering natural and new-to-nature pharmaceuticals, drug delivery agents and potential immunostimulants.

Phytochemistry and Biological Activities of Agrostemma Genus-A Review.

The family Caryophyllaceae comprises more than 2600 species spread widely across all the continents. Their economic importance is mainly as ornamentals (carnation) and as weeds in agriculture. Some species have been used traditionally (and some are still) in herbal medicine or as emulsifiers in food processing. These applications are based on the high content of triterpenoid saponins. Typical for this family are also ribosome-inactivating proteins (RIPs), which are potentially highly toxic. Agrostemma githago L. (common corncockle) was historically considered a serious toxicological hazard owing to cereal grain contamination by its seeds. Notwithstanding, it was also recommended as a drug by various herbalists. In this review, the literature was searched in the PubMed, Google Scholar, and Scopus databases for papers focused on the chemical composition and bioactivity of the two accepted species of the Agrostemma genus. This systematic review adhered to the Preferred Reporting Items for Systematic Reviews and MetaAnalysis (PRISMA) guidelines. Current research reports the cytotoxicity against neoplastic cells; the protection against oxidative stress; the suppression of Leishmania major culture growth; the inhibition of protein synthesis; and the antiviral, anti-angiogenic, and antihypercholesterolemic activities of common corncockle. The future prospects of using A. githago saponins as adjuvants in drug formulations and enhancing the cytotoxicity of RIPs are also discussed.

A Note on the Family Caryophyllaceae, with Special Reference to the Indian Species

This note provides with the general features of the family with discussions on the geographical distribution, phylogeny and systematic position, garden plants, and the medicinal and economic uses of a few species of the family. A detailed enumeration and description of taxa will be published later on in the "Flora of India".

A Catalogue and Lectotypification of the Type Materials of some Taxa of the Family Caryophyllaceae Available at the Calcutta Herbarium (cal.)

A list of type materials of Caryophyllaceae available at the Central National Herbarium, Calcutta is provided and two new combinations are proposed.

Assessment of Genetic Diversity and Relationships among Gypsophila and Silene Species from Turkey based on SRAP Markers

Gypsophila (Gypsophila L.) is a member of the Caryophyllaceae family and its genus consists of approximately 150 species. Some of several species are grown commercially for a variety of uses herbal medicine, and food. Its most common use is as cut flower worldwide. Gypsophila species are native and widely distributed in Turkey that is the main genetic resource center. In this study, firstly Gypsophila L. genotypes were collected from native areas in Turkey. Secondly, genetic diversity using molecular markers were provided valuable information for breeding programs and strategies of germplasm conservation. Sequence-related amplified polymorphism (SRAP) as a molecular markers were used to determine diversity and relationships among 41 Gypsophila (Caryophyllaceae) genotypes including 13 species (G. viscosa, G. simonii, G. venusta, G. bicolor, G. simulatrix, G. bitlisensis, G. germanicopolitana, G. perfoliata, G. arrostii, G. eleganas, G. paniculata and G. aucheri) and 2 Silene types (S. vulgaris and Silene spp.) as outgroups. As results, twenty primer combinations were produced 153 scorable fragments, and also all markers were showed 100% polymorphism for 43 genotypes. The significance of the resulting dendrograms was verified calculating the cophenetic correlation r values (r ≥ 0.80) for SRAP markers. The Gypsophila and Silene species were clearly grouped according to subspecies and by region. Results indicated that SRAP markers were useful for investigating diversity and relationships among Gypsophila germplasm. Additionally, this data could be used for development of new Gypsophila varieties in the breeding programme.

Effect of two different base cuts and indole-3-butyric acid (IBA) doses on rooting of stem cuttings of wild Silene compacta Fischer

Silene compacta Fisch. ex Hornem., which grows naturally, is a plant species with high ornamental plant potential in the Silene L. genus of the Caryophyllaceae family. Vegetative propagation is one of the important ways to preserve naturally growing and cultivated plant species. In this study, the effect of two different cutting areas of the cutting base (above the node-below the node) and five different indole-3-butyric acid (IBA) doses (control, 500, 1000, 2000 and 4000 ppm) on the rooting of stem cuttings taken from individuals of the natural S. compacta species were investigated. IBA was applied to the cuttings’ bases in a commercial rooting powder. Perlite+peat mixture at a ratio of 1:1 (v/v) was used in the rooting medium. Survival rate, rooting percentage, blooming rate, root number, root length, seedling height, seedling stem diameter, side shoot number, shoot length and shoot stem diameter were evaluated. The evaluation was made one month after the start of the experiment. As a result, the highest survival rate in cuttings was obtained from above-node control group (100%) and under-node 2000 ppm IBA (95%) application, and the highest rooting percentage was obtained from under-node 2000 ppm IBA (68%) and above-node control group (65%), respectively. Based on the results, it can be concluded that different cuttings of the cutting base and IBA concentrations have different effects on the rooting success of the S. compacta species with stem cuttings.

Family Caryophyllaceae in the flora of Central Kazakhstan: species composition, spreading, practical use

This article analyzes the species composition of the family Caryophyllaceae of the flora of Central Kazakhstan within the Karaganda and Ulytau regions, the distribution of identified taxa, ecobiomorphological features and phytoprotective status. It was revealed that the family Caryophyllaceae includes 80 species from 18 genera, which is 44.4% of the species flora of this family of Kazakhstan and 7.2% of the total number of species of the flora of Central Kazakhstan. The leading genera by number of species are Silene L. (28 species) and Dianthus L. (11 species). Endemic species are 2 taxa. Phytoprotective status has 1 species, which is included in rare and endangered plants of Karaganda region. In relation to moisture conditions, mesophytes (12 species) prevail among the described taxa, xeromesophytes (19 species) are in the second place, and mesoxerophytes (16 species) are in the third position. Analysis of life forms showed predominance of herbaceous perennial (60 species) and annual (16 species) forms. Among the species of the family Caryophyllaceae, the following economically valuable groups were noted: weedy, medicinal, poisonous, and suitable for perfumery and cosmetic purposes, ornamental, technical, fodder and honey-bearing groups.

Response of Soil Microorganisms and Phenolic to Pseudostelariae heterophylla Cultivation in Different Soil Types

Pseudostellaria heterophylla of the Caryophyllaceae family is an important medicinal herb in traditional Chinese medicine, but it encounters continuous cropping obstacle during cultivation. This study aimed to study the differences in the response of Pseudostellaria heterophylla cultivation to soil microorganisms and phenolic substances in different types of soil (Loamy soil and Clayey soil). The analysis of soil metabolites using HPLC-ESI-Q/TOF-MS and HPLC-DAD techniques revealed that the content of various phenolic components in the rhizosphere soil increased significantly after Pseudostellaria heterophylla cultivation. Pseudostellaria heterophylla cultivation in yellow soil could increase the abundance of Proteobacteria, Ascomycota, and Thermoplasmatota, while in brown soil, the abundance of Ascomycota significantly decreased. In addition, Pseudostellaria heterophylla cultivation could increase the α-diversity of bacteria and fungi in yellow soil, while reducing the α-diversity of archaea, and the impact on diversity in brown soil was relatively small. Correlation analysis showed that phenolic compounds were more likely to regulate the genera Bradyrhizobium, Calcarisporiella, Boothiomyces and Methanocella. Pseudostellaria heterophylla may regulate rhizosphere microbial diversity and community structure by secreting phenolic. However, in different types of soil environments, the response mechanism of soil microorganisms to Pseudostellaria heterophylla planting might vary. Our study provides new insights that the variability in the response of different soil types to Pseudostelariae heterophylla cultivation should be taken into account when developing strategies for reducing continuous cropping obstacles in medicinal plants.

PROPAGATION OF TRANSYLVANIAN ENDEMIC AND ENDANGERED PLANT SPECIES

Climate change has already caused irreversible stock changes in species distributions in many parts of the world. Species are lost and disrupted because they are highly dependent on changes in temperature. The aim of the experiment is to develop cultivation techniques for wild perennial plants of ornamental value that are rare, protected or highly endangered, thus helping to maintain these plant populations while increasing the number of ornamental plants grown. As plant material we have collected the seeds of Achillea ptarmica, Achillea impatiens, Achillea x girgioensis, Allium obliquum, Silene zawadskii, Dianthus petraeus ssp. orbelicus, and Centaurea triumfettii in two different years. The collected seeds were sown in spring and autumn, alongside these the germination was determined also in Petri dishes. From our results could be determined that at the Caryophyllaceae family both genus seeds germinated reasonably good, in the case of the plants belonging to the Asteraceae family the seeds obtained different results, furthermore at the A. obliquum the germination was low. The present experiment offers the possibilities to multiply these endangered plant species, and to improve the impoverished genetic stock.

Phenolic characterization, antimutagenic, antioxidant and antibacterial capacities of seven endemic Dianthus species from Turkey

Dianthus genus, belonging to the family Caryophyllaceae, encompasses over 300 species. Various Dianthus spp. have long been utilized in traditional medicine for the treatment of a wide range of conditions including chronic pain, urinary infections, carbuncles, menostasis, gonorrhea, cough, liver diseases, and certain types of cancer. The objective of this study was to determine the biological activities (antimutagenicity, antibacterial and antioxidant), total phenol-flavonoid content and individual phenolic constituents of methanolic extracts of two different parts [FC (flower+capitulum) and LS (leaf+stem)] of seven different endemic Dianthus spp. (D. ancyrensis, D. aticii, D. balansae, D. carmelitarum, D. kastembeluensis, D. lydus and D. leucophaeus) found in Bolu, Turkey. In the antimutagenicity tests, D. carmelitarum, D. ancyrensis. D. kastembeluensis and D. lydus demonstrated higher inhibition effect against the mutagenic activity of sodium azide (NaN3) and 4-nitro-o-phenylene diamine (NPD) on Salmonella typhimurium TA 100 and TA 98 strains, respectively. D. carmelitarum-LS displayed the highest antimutagenic effect against the mutagen (base-substitution) on the TA 100 strain, while D. carmelitarum-FC exhibited the highest inhibition against mutagenic activity (frame-shift mutagenesis) by 4-nitro-o-phenylene diamine (NPD) on S. typhimurium TA98 strain. Among the tested 17 bacteria, only 4 bacteria (Staphylococcus epidermidis, S. aureus, Streptococcus pyogenes and Klebsiella pneumonia) demonstrated sensitivity to five different Dianthus spp. D. leucophaeus exhibited moderate antibacterial activity against S. epidermidis. Furthermore, S. pyogenes was quite susceptible to D. kastembeluensis, D. aticii and D. lydus. The most powerful radical scavenging activity was observed with D. balansae-LS. Consistent with this result, D. balansae-LS showed the highest total phenolic content. D. aticii-LS had the highest content of total flavonoid. U-HPLC analysis revealed that rutin, phloridzin, ferulic acid and chlorogenic acid were the most dominant compounds in endemic Dianthus spp. These findings highlight the potential of these plants as valuable natural sources for pharmaceutical and nutraceutical applications.

Complete chloroplast genomes of Cerastium alpinum, C. arcticum and C. nigrescens: genome structures, comparative and phylogenetic analysis

The genus Cerastium includes about 200 species that are mostly found in the temperate climates of the Northern Hemisphere. Here we report the complete chloroplast genomes of Cerastium alpinum, C. arcticum and C. nigrescens. The length of cp genomes ranged from 147,940 to 148,722 bp. Their quadripartite circular structure had the same gene organization and content, containing 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Repeat sequences varied from 16 to 23 per species, with palindromic repeats being the most frequent. The number of identified SSRs ranged from 20 to 23 per species and they were mainly composed of mononucleotide repeats containing A/T units. Based on Ka/Ks ratio values, most genes were subjected to purifying selection. The newly sequenced chloroplast genomes were characterized by a high frequency of RNA editing, including both C to U and U to C conversion. The phylogenetic relationships within the genus Cerastium and family Caryophyllaceae were reconstructed based on the sequences of 71 protein-coding genes. The topology of the phylogenetic tree was consistent with the systematic position of the studied species. All representatives of the genus Cerastium were gathered in a single clade with C. glomeratum sharing the least similarity with the others.

A Morphological Study of Some Silene L. Species of Caryophyllaceae family in Iraq

The current works included morphological features study (vegetative and floral parts) for five species of the genus Silene L., S. ampullata, S. chlorifolia, S. colorata, S. conoidea and S. longipetala belong to the family Caryophyllaceae, samples were collected from various areas of northern Iraq. Confirmation in the description was on the most critical parts of the plant, including the shape of the leaves, length, and shape of the calyx tube and venation, the modification shapes of coronal scales petals and appendages, anther ornamentation and structures of pistils. Images enhanced the morphological characteristics under study. Information on the locations and dates of the collection was also confirmed.

In silico evaluation of isoflavones for its galactagogue activity in promoting lactation

Breastfeeding is crucial for the health of both mothers and infants, providing both short-term and long-term benefits. Breast milk contains all the necessary nutrients for both the mother and child. Unfortunately, many women are experiencing a low milk supply, resulting in infants not receiving sufficient immune support. To increase milk production, there are various herbal and pharmaceutical galactagogues available. Plants in the Caryophyllaceae family contain phytoestrogen, a compound that helps stimulate milk production. Isoflavone is one type of phytoestrogens. Isoflavones have many properties like bactericidal, fungicidal and anti-ringworm and are used to treat breast and prostate cancer and bone-related diseases. Spergula arvensis L., a member of the Caryophyllaceae family, is rich in isoflavones. Nine specific compounds were chosen due to their connection to mammary gland function. A preliminary in silico study was conducted to examine the interaction between isoflavones and prolactin receptors through molecular docking. The compound quercetin, an isoflavone, showed a better binding affinity to the receptor.

An Accurate Phenotypic Study of The Foliar Epidermis for Some Species of Silene L. From Caryophyllaceae Family in Iraq

In the present research work, detailed micromorphological features study (investigated for foliar epidermal anatomy) for five species of the genus Silene L., belonging to the family Caryophyllaceae. One of the essential anatomical characteristics of the leaf in this genus observed two basic stomata types on both adaxial and abaxial epidermis, diacytic and anisocytic, from five species, three species have distinguished a diacytic type, exception two species S. chlorifolia and S. longipetala appeared to have both a diacytic and anisocytic, also has astrocytic crystals within epidermal cells. Also in the present work, 3 species out of 5 have trichomes on their both surfaces, observed having them multicellular uniseriate. All the taxa were the distinguished shape of the guard cells as reniform-like from a full short reniform in all species. So the general shape of ordinary epidermal cells, anticlinal wall of the epidermal cells, type of stomata cells, collar shape marginal of guard cells, and trichomes. Images enhanced the micromorphological characteristics under study. Information on the locations and dates of the collection was also confirmed. This study revealed that the micromorphological features of the family are taxonomically signi?cant in the accurate identi?cation of species.

Varietal Evaluation of Carnation (Dianthus caryophyllus) in Naturally Ventilated Polyhouse under Prayagraj Agro-climatic Condition

Carnation, scientifically known as Dianthus caryophyllus, is a popular and attractive flowering plant that belongs to the Caryophyllaceae family. Native to the Mediterranean region, carnations are now cultivated all over the world for their beauty and fragrance. Therefore, present investigation was carried out at the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh during the Winter-2022-23 with a view to determine the performance of different varieties of carnation for its growth and flowering under ventilated polyhouse. Under this experiment, overall, 6 varieties were used comprising of variety Cerventes, Master, Magno, Baltico, Realism and Kiro. It was concluded that variety Baltico (white) reported significantly better performance compared to other varieties, in terms of Growth parameters like plant height was seen in cervantes (25.19 cm), number of leaves were maximum in master (20.94), number of internodes was highest in realism (18.49), number of branches were seen to be maximum in baltico (4.47), Kiro was found to be less suited variety for Prayagraj agro climatic condition due to metabolic activity. Flowering parameters like days to first flower bud break (65.33 days), days taken for first flower bud opening (66.00 days) was seen in Master, Flower Stalk length was seen highest in Realism (40.80 cm), number of flowers per plant was seen in cervantes (5.17), number of days taken for flower senescence on plant was seen highest in kiro (15.40 days), the vase life in carnation was seen to be the highest in master (0.40 days).

Taxonomic significance of anatomical and micro-morphological characteristics of Silene takesimensis Uyeki & Sakata.

This study used the foliar epidermal anatomical characteristics through microscopic techniques to compare the foliar anatomy of Silene takesimensis Uyeki & Sakata (Caryophyllaceae). The species is endemic to South Korea. This study examined the foliar epidermal anatomical traits. The leaf morphological traits of the species are essential to distinguish the species from other taxa. The comparative systemic significance of the character species was examined. The epidermal cell shape, the epidermal cell wall, and the number of lobes per cell were among the distinctive foliar anatomical characteristics. The variations in quantitative characteristics were significant. The systematics of the genus Silene were supported by various microscopic methods. The foliar epidermal anatomical characteristics of the endemic species S. takesimensis have significant taxonomic characteristics to differentiate the species. RESEARCH HIGHLIGHTS: Silene takesimensis, a member of the Caryophyllaceae family, has been studied in-depth. Valuable insights and knowledge were obtained about the unique characteristics and behaviors of Silene takesimensis using SEM.

ANTIMICROBIAL ACTIVITY OF BUTANOL COMPLEXES AND ISOLATED SECONDARY METABOLITES OF SOME SPECIES OF GENUS SILENE OF THE FAMILY CARYOPHYLLACEAE

Extracts of 9 species of the Caryophyllaceae family were screened for antimicrobial activity on the example of 17 types of strains of microorganisms. The inhibitory activity of 7 butanol extracts has been shown against a number of pathogenic strains belonging to the species Mycobacterium smegmatis, Phroteus mirabilis, Serratia marcescens, Staphylococcus aureus, Streptococcus faecalis. The extracts studied were most effective on the bacteria Bacillus cereus and Bacillus subtilis. It was found that all the studied plant species of the genera Lychnis and Silene contain ecdysteroids and flavonoids. The isolated individual compounds contained in all the studied species showed antimicrobial action on strains of a number of bacteria: the ecdysteroid 20-hydroxyecdysone suppressed the growth of the strain of R. mirabilis, while the flavonoid shaftoside inhibited strains of bacteria S. aureus, B. cereus, B. subtilis, R. mirabilis, M. smegmatis. Gram-negative bacteria Salmonella typhimurium, Klebsiella pneumoniae, Shigella sonnei, Pseudomonas aeruginosa are not susceptible to the antimicrobial action of extracts under experimental conditions.
 Determination of antimicrobial activity was carried out by methods – diffusion and joint incubation of plant extracts and cells of test strains of microorganisms in a liquid nutrient medium LB at the ratio sample : medium 1 : 2 and 1 : 1. Increase in the concentration of secondary metabolites of ecdysteroids and flavonoids in experiments with butanol complexes of substances with a sample ratio the 1 : 1 environment led to an increase in antimicrobial activity up to the complete inhibition of Gram-positive bacteria B. subtilis and B. cereus by extracts of plant species Silene graefferi, S. colpophylla, S. sendtneri, S. linicola, S. jenisseensis S. viridiflora (seeds) and individual shaftoside.