Carabidae Research Articles

Niche differentiation of arthropods and plants along small-scale gradients in temporary wetlands (kettle holes)

Small temporary wetlands, like kettle holes, provide many valuable ecosystem functions and serve as refuge habitats in otherwise monotonous agricultural landscapes. However, the mechanisms that maintain biodiversity in these habitats are still poorly understood. In this study, we investigate how three taxa (vascular plants, ground beetles and spiders) respond to small-scale flooding and disturbance gradients in kettle holes as well as kettle hole area. For this purpose, we determined total, hygrophilic and red list species richness for all taxa and activity density for arthropods along transects extending from the edge towards the center of kettle holes. Furthermore, we calculated the community-weighted mean body size for arthropods and seed mass for plants as surrogates for the ability to respond to disturbance. Our analyses revealed that in particular plants and ground beetles showed strong responses along the small-scale spatial gradient. Total plant species richness decreased towards the center, while hygrophilic plant species increased. In contrast, both total and hygrophilic species richness of ground beetles increased towards the center. Spiders showed similar responses as ground beetles, but less pronounced. We found no evidence that disturbance at the edge of kettle holes leads to smaller body sizes or seed masses. However, arthropods in adjacent arable fields (one meter from the kettle hole edge) were particularly small. Kettle hole area had only weak effects on plants, but not on arthropods. Our study indicates that differences in the depth at the drier edge and the moist, regularly flooded center have a large and taxon-dependent influence on the species composition. Therefore, small-scale heterogeneity seems to be an important predictor for the maintenance of species diversity.

Regional sets of diverse, specialized ground beetle and plant assemblages recolonise China’s restored temperate forest landscapes

Following the historic destruction of China’s forests, large protected forest landscapes have been reestablishment across the country in recent decades. Currently, the species assemblages inhabiting these landscapes remain widely unknown. Here, we aim to establish whether the new temperate forest landscapes in Eastern China, composed of plantation, secondary and remnant mature forest patches, are dominated by widely distributed habitat generalists, widely distributed habitat specialists, or by sets of highly localized forest habitat specialist species, using vascular plants and ground beetles as case studies. We sampled plants and ground beetles on 159 plots representing different forest types distributed across ten study regions. The Chord-normalized expected species shared (CNESS) dissimilarity for beetles and Jaccard dissimilarity for plants were calculated to establish species’ turnover patterns, while we used structural equation and multiple regression models for dissimilarity matrices to establish the relative importance of location, elevation and biotic interactions in explaining large-scale distribution patterns of species assemblages. Both taxa showed highly regionalized recruitment, with plant communities responding strongly to latitude, and to geographic location in general, while the composition of ground beetle communities appeared strongly governed by both the composition of the vegetation and study plot location. Both assemblages showed a distinct lack of widespread habitat generalists. Our results demonstrate for the first time that local assemblages of forest specialist species appear to have survived last century’s deforestation in unknown refugia before successfully re-colonizing China’s restored forest ecosystems. We argue that restored forest landscapes can play a significant role in the conservation of temperate China’s biodiversity at both local and regional scales.

New records of ground beetle species (Coleoptera, Carabidae) from Khabarovskii Krai, Russia

New records of ground beetle species (Coleoptera, Carabidae) from Khabarovskii Krai, Russia

CLIMATIC FACTORS CAN DIFFERENTLY AFFECT BODY SIZE IN CLOSELY RELATED SPECIES (THE CASE STUDY IN GROUND BEETLES)

The study of body size variation in the altitudinal gradient showed the presence of shifts in the morphometric characteristics of ground beetle populations under the influence of bioclimatic factors. Ground beetles were collected on the Barguzinsky Ridge (Russia, Buryatia) in eight characteristic biotopes in four high-altitude sections: on the coast of Lake Baikal, low, middle and high mountains (458-1667 m above sea level). At each site in 2004–2017, we carried out a quantitative account of ground beetles and recorded the main climatic parameters using thermochrons, sedimentary cylinders, soil thermometers, and a snow gauge. We took two common species – Carabus odoratus and Pterostichus montanus (2200 specimens) for morphometric analysis. The six features of the body organs – the length and width of the elytra, the length and width of the pronotum, and the length of the head and distance between the eyes were measured. Using linear modeling, we investigated how climatic variables affected body size in the studied species. Comparing the reaction of the two species to hydroclimatic parameters, we noted that the reaction was directly opposite to temperature indicators, and the similar to factors that depend on humidity. The higher was the soil temperature at a 5 cm depth, the greater was the length of the elytra, pronotum, and head in C. odoratus, but the same characters were smaller in P. montanus. High population density led to body size decrease in C. odoratus, but P. montanus responded in lesser degree. By genus, C. odoratus turned out to be more sensitive to changes in bioclimatic factors.

THE STRUCTURE ASSOCIATIONS OF CARABIDAE (COLEOPTERA) IN WATERSHED LINDEN FORESTS OF UDMURTIA AND THEIR CHANGES UNDER ANTHROPOGENIC INFLUENCE

The composition and structure of peculiar complexes of ground beetles (Carabidae) of watershed linden forests of Udmurtia were studied and analyzed in comparison with complexes of disturbed forests with a predominance of linden in the green zone of Izhevsk. It has been shown that about 40 species of the family inhabit the primary watershed linden forests, among which forest mesophiles and mesohygrophiles predominate, including a number of rare and specially protected species of Siberian and Ural origin ( Carabus schoenherri Fischer von Waldheim, 1820, C. aeruginosus hermanni Mannerheim, 1827, Carabus henningi Fischer von Waldheim, 1817, Pterostichus urengaicus Jureček, 1924, P. uralensis krasnobaevi O.Berlov & Tilly, 1998) and representatives of European fauna ( Carabus glabratus Paykull, 1790, C. hortensis Linnaeus, 1758, Pterostichus aethiops (Panzer , 1796). Complexes of recreational and, especially, park linden forests are characterized by low species richness and trivial species composition, which is based on common eurybiont and meadow-field species, as well as an invasive European species - Carabus nemoralis Müller, 1764.

Effects of agricultural landscape structure and canola coverage on biochemical and physiological traits of the ground beetle Poecilus cupreus.

The intensifications in the agricultural landscape and the application of pesticides can cause adverse effects on the fitness of organisms in that landscape. Here, we investigated whether habitats with different agricultural pressures influenced acetylcholinesterase (AChE) activity - a biomarker for exposure to pesticides, respiration rate, and resistance to starvation in the ground beetle Poecilus cupreus. Two differently structured landscapes were selected for the study, one dominated by small (S) and another by large (L) fields. Within each landscape three habitat types were selected: in the S landscape, these were habitats with medium (M), small (S) and no canola (meadow, 0) coverage (i.e., SM, SS, S0), and in the L landscape habitats with large (L), medium (M) and no canola (meadow, 0) coverage (i.e., LL, LM, L0), representing different levels of agricultural pressure. The activity of AChE was the highest in beetles from canola-free habitats (S0 and L0), being significantly higher than in beetles from the SM and SS habitats. The mean respiration rate corrected for body mass was also the highest in S0 and L0 beetles, with significant differences between populations from L0 vs. SS and from S0 vs. SS. Only beetles from S0, SS, L0, and LM were numerous enough to assess the resistance to starvation. Individuals from the LM habitat showed better survival compared to the canola-free habitat in the same landscape (L0), whereas in S landscape the SS beetles survived worse than those from S0, suggesting that characteristics of L landscape may lead to developing mechanisms of starvation resistance of P. cupreus in response to agricultural pressure.

Temporal changes in the beetle and spider communities in a Hessian (German) strict forest reserve

In the context of the Hessian strict forest program, the reserve Hasenblick was set aside from forest management in 1988. The program aims to document the faunas of forest reserves in unprecedented detail, using a set of many different sampling methods. Here data on ground dwelling spiders and beetles that were sampled with pitfall traps in 2000 and 2001 and again in 2012 and 2013 are analyzed to assess temporal changes. In light of putative insect declines, we hypothesized a significant decrease in abundance, biomass, diversity, and trait composition of the arthropod communities. No substantial changes in community trait composition were observed in any species group. Abundance, biomass, and functional diversity of beetles and spiders were higher in the second survey, with exception of the beetle biomass, when Anoplotrupes stercorosus was excluded, and the functional diversity of spiders showed only a tendency to be higher in the second survey. Additionally, the extrapolated number of ground dwelling beetle species was higher during the second survey. However, in all tested measures, the observed differences between the surveys were not significantly higher than differences observed between consecutive years. Therefore, we are not able to detect directed long-term trends of (functional) diversity or biomass in our dataset. The results rather indicate high stability of the arthropod communities in this naturally developing forest, although short-term fluctuations in populations are high. And while climatic factors affected abundance and biomass of beetles and spiders overall, the timespan of about 10 years may be too short to clearly detect effects of climate change or changes in forest structure on the trait composition of the beetle or spider communities.

Species composition and ecological structure of ground beetle communities (Coleoptera, Carabidae) in reclaimed rock dumps in the south of Western Siberia

Coal mining has a strong negative impact on the environment. It transforms natural landscapes into technogenic ones with high rock embankments and deep depressions, i.e., dumps and quarries. Such areas are good model sites for studying primary successions. Using the example of the Krasnobrodsky coal mine, this paper investigates the ecological and faunal structure of the ground beetle communities which inhabit the rock dumps of various ages in the forest-steppe zone in the south of Western Siberia. A total of 125 ground beetle species from 38 genera were collected. It was also established that fresh dumps were quickly populated by ground beetles. The basis of the taxocene is formed by species with high ecological plasticity, confined to anthropogenic biotopes. According to the Principal Component Analysis (PCA), ground beetle communities in dumps differ from native reference ecosystems. The Jaccard similarity index between 2- and 7-year-old dumps was 54%, while for the control zone and the site at the foot of the 25-year-old dump, this value was 68%. During 35 years of succession, the species composition of carabidocenoses varied, while the species abundance and dynamic density decreased (r = -0.78, p < 0.001). A wide range of life forms has been established for ground beetles. This indicates the active development of ecological niches, formed in technogenically transformed territories. This study demonstrates that the time interval of 35 years used for consideration is insufficient for recovering ground beetle communities to a climax state, typical to the ecosystems of a corresponding forest-steppe zone.

Exploring Prescribed Fire Severity Effects on Ground Beetle (Coleoptera: Carabidae) Taxonomic and Functional Community Composition

Prescribed fire is a management tool that is frequently used to foster biodiversity. Simultaneously, insects that provide essential ecosystem services are globally declining. Within the pyroentomology literature, there are mixed reports of positive and negative effects that prescribed fires have on insect communities. This is likely due to not accounting for fire heterogeneity created by fire severity. To better understand prescribed fire severity effects on insect communities, we used multispectral reflectance data collected by Sentinel-2 to methodically quantify prescribed fire severity and compared ground beetle (Coleoptera: Carabidae) taxonomic and functional community composition responses between an unburned site and two burned sites with contrasting fire impacts. We found 23 ground beetle species and used 30 morphological, physiological, phenological, and ecological functional traits for each species. We found that our moderate fire severity site had different taxonomic and functional community compositions from both our unburned and high-severity sites. Surprisingly, we did not find a strong difference in taxonomic or functional ground beetle composition between our unburned and high-severity sites. Our results encourage future pyroentomology studies to account for fire severity, which will help guide conservation managers to make more accurate decisions and predictions about prescribed fire effects on insect biodiversity.

The genome sequence of a ground beetle, Leistus spinibarbis (Fabricius, 1775).

We present a genome assembly from an individual male Leistus spinibarbis (a ground beetle; Arthropoda; Insecta; Coleoptera; Carabidae). The genome sequence is 235.1 megabases in span. Most of the assembly is scaffolded into 23 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 15.82 kilobases in length. Gene annotation of this assembly on Ensembl identified 23,576 protein coding genes.

The effects of heterogeneity created by treefall, landslide, and stream on ground beetle assemblages in a primary beech forest

Old-growth forests play essential roles in maintaining biodiversity and providing ecosystem services. While heterogeneity produced by various natural disturbances and micro-topography is an integral part of old-growth forest ecosystems, the effects of such natural disturbances and heterogeneity on arthropod diversity in old-growth forests are not fully understood. In this study, we evaluated how the heterogeneity produced by treefalls, landslides, and streams influences ground beetle diversity in an old-growth beech forest in the Tadami Biosphere Reserve, Japan. Fourteen plots composed of five forest interior, three treefall gaps, three landslides, and three along a stream were established, with 15 pitfall traps installed in each plot. Traps were opened for two days in each season. The results showed that canopy openness was the highest in landslide gaps, whereas litter depth was the lowest along the stream. Species richness was lowest along the stream but was similarly high in the other three habitat types. The activity density of beetles was also lowest along the stream, but highest in the landslide gaps. Landslide gaps and streams had distinct species compositions and several indicator species, respectively, indicating that soil disturbances involved in landslides and repeated overflow by snowmelt and heavy rain along the stream provide specific environmental conditions that differ from the forest interior and treefall gaps. This study demonstrates that heterogeneity derived from natural disturbances and streams enhances biodiversity in old-growth forests. These findings can be used to develop management measures that mimic natural disturbances in order to conserve biodiversity in managed forests.

Epigeic Carabids (Coleoptera, Carabidae) as Bioindicators in Different Variants of Scots Pine Regeneration: Implication for Forest Landscape Management

Maintaining biodiversity is one of the factors determining the proper functioning of ecosystems, especially those with high human impact. Ground beetles, as bioindicators, are particularly valuable in understanding ecosystem responses and sustainability in forest and landscape management. Focusing on the regeneration of pine forests, this study aimed to describe ground beetle assemblages on Scots pine natural and artificial regeneration in northeastern Poland. This study was conducted between 2016 and 2018. Pitfall traps were set up for catching epigeic carabids on previously prepared research plots designated for natural and artificial pine regeneration. The research areas included three variants: N—natural pine regeneration, plots without soil preparation; NP—natural pine regeneration with traditional soil preparation by ploughing; and A—artificial pine regeneration with ploughing. Four plots as replicants were selected in each experimental variant, with six pitfall traps in the transects running through the centre of each study plot. In each year of the study, 11 samples were collected from each plot; overall, 33 samples per plot were collected during the three years. As a result of this study, 26,654 ground beetle individuals belonging to 89 species were caught during the three-year observation. Natural regeneration without soil preparation (N) was the most favourable in terms of the occurrence of stabilised assemblages of ground beetles. However, the remaining methods of pine regeneration, on a multi-annual scale (2016–2018), contributed to the increase in the number of ground beetles but also provided high variability in assemblage composition, diversity indices, and life-history traits. Thus, in the early stage of pine regeneration, each of the examined variants of pine regeneration can be used without fear of causing damage to carabid populations. However, further studies are required to investigate the effect of different pine regeneration types on carabid beetle assemblages over a longer period.

FAUNA AND SPATIAL DISTRIBUTION OF GROUND BEETLES (COLEOPTERA: CARABIDAE) ON THE CHEKHOV MOUNTAIN, SAKHALIN ISLAND

Totally 61 species of ground beetles are known from the Chekhov Mountain (Susunai Range, Sakhalin Island), of which 21 species are recorded from here for the first time. The results of collecting ground beetles in 9 model plots in 2021-2022 are analyzed. Three complexes of ground beetle communities are identified: I – floodplain low-mountain; II – low-mountain forest and meadow; III – alpine.

Environmental stress responses and adaptive evolution in the alpine ground beetle Nebria vandykei

Abstract The oscillating glacial–interglacial climate has had well-characterized effects on alpine species, driving rapid distributional and demographic shifts that have led to lineage diversification. It is unclear whether adaptive evolution has occurred during these rapid demographic changes, because strong genetic drift can overcome the force of selection. Here, using the alpine ground beetle Nebria vandykei, we test for evidence of adaptive evolution. Initially, we explore the genetic pathways induced during environmental stress responses through RNA sequencing, showing that cold, heat and desiccation stress activate a largely non-overlapping set of molecular pathways. Using additional transcriptome sequencing, we estimate the evolutionary relationship of N. vandykei to related species in the subgenus Catonebria and several outgroups. Phylogenetic analyses suggest that a history of admixture or very rapid diversification underlies the evolution of N. vandykei. Finally, using tests for selection polarized by high- and low-elevation relatives, we demonstrate selection acting on stress response pathways and on pathways known to function in tolerance to cold and hypoxic environments. These results support the role of environmental adaptation in alpine species despite rapid demographic change, while demonstrating that admixture might play a key role in facilitating adaptive diversification of alpine species.

A CHECKLIST OF SPECIES OF THE SOME COLEOPTERAN FAMILIES (COLEOPTERA) OF ROCK DUMPS IN KUZBASS

The results of processing the material collected by pitfall traps on the rock dumps of two coal mines in the Kemerovo oblast (Kuznetsk Basin) in the period from 2013 to 2022 are presented. Fifty beetles species of 14 families were found (Histeridae, Leiodidae, Silphidae, Geotrupidae, Trogidae, Lucanidae, Scarabaeidae, Byrrhidae, Elateridae, Dermestidae, Zopheridae, Tenebrionidae, Anthribidae, Curculionidae), while representatives of the families Carabidae and Staphylinidae were not taken into account. For some of the discovered species, new findings were identified in the Kuznetsk Basin.

Historical Climate Change Dynamics Facilitated Speciation and Hybridization Between Highland and Lowland Species of Baripus Ground Beetles From Patagonia

One of the largest beetles of Patagonia, Baripus (Cardiophthalmus), includes 20 currently described species. Its distribution ranges from the tip of Patagonia, on Tierra del Fuego Island, to isolated patches along the Andes and extra-Andes mountains in northern Patagonia on the Payunia at >3000 m elevation. Here, using RADseq data, evidence is found of mixed ancestry in different lineages. Phylogenetic network reconstruction shows two hybridizing edges between lowland and highland species. Using environmental niche modeling, we show changes in geographic distribution of potential niches of species during the last glacial maximum compared to their present distribution. Increasing potential niche overlap among different species pairs possibly explains how lineages came into secondary contact, supporting the hypothesis of hybridization. In addition, morphological evolution is studied using geometric morphometrics on the network, and evidence of transgressive evolution has been found involving the pronotum shape, as well as highland/lowland habitat preferences. Finally, based on genomics and morphological data and using an integrative coalescent-based species delimitation approach, the separate evolution of two lineages in early stages of speciation is found. Taken together, dynamics of diversification of Baripus beetles in both space and time are discussed.

Knowledge of ground‐dwelling beetle communities in the tropical Andes: Gaps and trends

AbstractThe tropical Andes is one of the most biodiverse ecoregions on Earth, exhibiting high levels of species endemism. The tropical Andes is also one of the most vulnerable ecoregions worldwide due to a long history of anthropogenic transformations. One of the major obstacles for generating conservation plans is gaps in knowledge about the status and threats to many groups of Andean biodiversity, especially invertebrates. Focusing on three important beetle groups (i.e., Coleoptera: ground beetles, rove beetles and dung beetles) in the tropical Andes, we performed a systematic review of scientific literature retrieving 50 studies that allowed us to assess the degree of knowledge across the region, disturbance types, and biodiversity metrics used. We found a high representation of studies in Colombia, compared with other Andean countries. We also found a marked bias among taxonomic groups, topics or metrics evaluated, with dung beetles the most represented group, particularly in studies addressing forest conversion using metrics such as species richness or abundance. We reveal large gaps in information on the diversity of ground and rove beetles, and in the use of other diversity metrics. The lack of information across countries makes it difficult to generalize results and points to the need for more research, especially in under‐represented countries such as Bolivia and Venezuela. Including complementary response metrics, such as biomass, functional diversity or ecological functions, will improve the assessment of human impacts on tropical Andean beetle communities and wider ecosystems.

Quantitative assessment and seasonal incidence of soil invertebrates in okra (Abelmoschus esculentus L.) crop

The present study on population dynamics and diversity of soil invertebrates was carried out at Vegetable Research Farm, Punjab Agricultural University, Ludhiana during 2019. The study revealed that okra crop harboured 14 different soil invertebrate species belonging to nine orders and 13 families dominated by order coleoptera (five species) dominated by ground beetles. Interestingly, higher relative abundance (%) was recorded in order hymenoptera (80.72%) followed by polyzoniida (10.41%) among which higher dominance (%) was recorded in little black ants (80.78%). Species richness, species evenness, Shannon-Wiener index (H’) and Simpson’s index were calculated to be 14.0, 0.094, 0.098 and 0.051, respectively, indicating high degree of diversity among soil invertebrates in okra crop. Interestingly, an increase of 88.84 and 69.64% in population was recorded in root-knot and free-living/predatory nematodes at harvest time compared to the okra crop sowing time. A positive correlation (r=+0.06 to + 0.83) was recorded between soil temperature and soil moisture with soil invertebrate population. Okra crop fields survive soil fauna, which is a key factor for increasing soil fertility and productivity and its physical characteristics, which should be increased using soil amendments.

Nutritional Composition of House Cricket (Acheta domesticus) and Dung Beetle Larva (Oryctes boas) in Osun State: Implication to Dietary Improvement in Nigeria

Background: Edible insects and the act of eating insects (Entomophagy) are common across the globe. Feeding variations and species differences, among other factors, influence these individuals’ chemical and mineral components. The current study elucidated the nutritional composition of house cricket (Acheta do-mesticus) and dung beetle larva (Oryctes boas) collected in Osun State, Southwestern Nigeria. Methods: The nutritional composition of the two species of insects sampled randomly was determined following standard methods of the Association of Official Analytical Chemists. The ground crickets and beetle larvae were digested with HNO3 and HCl in a microwave digestion system; inductively coupled plasma-optical emission was used to determine the amounts of Calcium, Sodium, Potassium, Phosphorus, Magnesium, Iron, Copper, Manganese, and Zinc in the samples. Following a standard procedure, the amino acid profile was performed using a Biochrom30+ amino acid analyzer (Pharmacia-Biotech, Buckinghamshire, UK). Data obtained were expressed as means ± standard deviations of triplicate analyses and compared among the two insect species using Student’s t-test at p < 0.05. Results: Generally, the nutritional components of A. domesticus were higher than that of O. boas. The O. boas was not significantly higher (P> 0.05) in Iron (6. 83 ± 1. 80 mg/100 g dry matter) but significantly high-er (P< 0.05) in Magnesium (730. 94 ± 2. 64 mg/100 g dry matter) than A. domesticus. All Essential Amino Acids (EAAs) and Non-Essential Amino Acids (NEAAs) were present in the two insect species with varying concentrations. House cricket, however, has a significantly higher (P< 0.05) Arginine, Valine, Phenylalanine, Glutamine, and Glutamic Acid than O. boas. Conclusion: The two insects under this study are edible and of great nutritional values. The results of the nutritional composition of Acheta domesticus and Oryctes boas may serve as the basis for improvement in human food and feed formulation in Nigeria

Sensitivity of non-target groups of invertebrates to cypermethrin

Agrogenic pollution with pyrethroid insecticides has been impacting the structure of populations of terrestrial invertebrates, causing decline in their taxonomic diversity and tolerance to critical values of environmental factors. In a laboratory experiment, we evaluated the sensitivity of 46 non-target invertebrate species to cypermethrin. In most examined species, we observed correlation between the body parameters (length and weight of body) and tolerance to this insecticide. We determined that the greater body size of the invertebrates, the better their tolerance to cypermethrin. Differences in LD50 were the highest for groups of invertebrates with the body weight of 1.0–3.9 mg (1.9 ± 0.5 g/ha) and 16.0–63.9 mg (16.4 ± 3.2 g/ha). We observed a relashionship between the trophic specialization and sensitivity to the insecticide in phytophages and zoophages. Average LD50 values for phytophages were 2.1 ± 0.5 g/ha, much lower than for zoophages – 15.6 ± 3.3 g/ha. Among zoophages, the greatest tolerance to cypermethrin was demonstrated by ground beetles Carabus coriaceus L., Pterostichus niger (Schall.), P. melanarius (Ill.), Pseudoophonus rufipes (De Geer), and earwigs Forficula auricularia L. Analysis of various taxonomic groups of insects revealed the parameter to be 24.00 ± 4.66 for Carabidae, 8.60 ± 2.72 for Formicidae, and 0.23 ± 0.08 for Staphylinidae. Among the taxonomic groups we studied, the most sensitive to cypermethrin (LD50 = 0.002–0.99 g/ha) were Philonthus decorus (0.0029), Ph. rectangulus (0.0035), Ophonus rufibarbis (0.121), Oxytelus sculptus (0.124), Myrmica ruginodis (0.39), Aleochara lanuginosa (0.49), Carabus granulatus (0.51), Oxythyrea funesta (0.52), Tachinus signatus (0.55), Cixiidae sp. (0.56), Lygus pratensis (0.56), Carabus convexus (0.71), and C. hortensis (0.83). Lower sensitivity to cypermethrin (LD50 = 1.00–9.99 g/ha) was seen in Lasius fuliginosus (1.05), Pyrrhocoris apterus (1.28), Chortippus sp. 2 (1.96), Rhyparochromus phoeniceus (2.24), Phosphuga atrata (2.25), Chironomus plumosus (2.58), Labia minor (2.86), Graphosoma italicum (2.86), Hister fenestus (3.39), Cylindroiulus truncorum (3.61), Opilio saxatilis (3.71), Chortippus sp. 1 (3.94), Epaphius secalis (4.54), Lasius niger (4.77), Silpha carinata (4.84), Aphodius foetens (4.94), Porcellio laevis (5.68), Coreus marginatus (6.50), Leistus ferrugineus (7.39), and Lasius alienus (9.73). The most tolerant to cypermethrin (LD50 = 10.00–108.00 g/ha) were Calathus fuscipes (12.14), Limodromus assimilis (12.22), Trochosa terricola (12.55), Lithobius forficatus (13.98), Calathus ambiguus (20.85), Nebria brevicollis (23.20), Ponera coarctata (27.04), Megaphyllum sp. (29.01), Pseudoophonus rufipes (41.75), Pterostichus melanarius (45.78), P. niger (58.29), Forficula auricularia (80.57), and Carabus coriaceus (107.71). The differences we found in tolerance to cypermethrin ranged 100,000 times. This evidences the necessity of further research of taxonomic differences in tolerance of invertebrates to cypermethrin.