Freshwater Fish Family Research Articles

Structure of supporting elements in the dorsal fin of percid fishes

The dorsal fin is one of the most varied swimming structures in Acanthomorpha, the spiny-finned fishes. This fin can be present as a single contiguous structure supported by bony spines and soft lepidotrichia, or it may be divided into an anterior, spiny dorsal fin and a posterior, soft dorsal fin. The freshwater fish family Percidae exhibits especially great variation in dorsal fin spacing, including fishes with separated fins of varying gap length and fishes with contiguous fins. We hypothesized that fishes with separated dorsal fins, especially those with large gaps between fins, would have stiffened fin elements at the leading edge of the soft dorsal fin to resist hydrodynamic loading during locomotion. For 10 percid species, we measured the spacing between dorsal fins and calculated the second moment of area of selected spines and lepidotrichia from museum specimens. There was no significant relationship between the spacing between dorsal fins and the second moment of area of the leading edge of the soft dorsal fin.

Partner swapping and cheating: why do different minnow species spawn together?

Partner swapping and cheating: why do different minnow species spawn together?

†Tugenchromis pickfordi, gen. et sp. nov., from the upper Miocene—a stem-group cichlid of the ‘East African Radiation’

ABSTRACT The highly diverse tropical freshwater fish family Cichlidae is sparsely represented in the fossil record. Here we describe the new cichlid †Tugenchromis pickfordi, gen. et sp. nov., from the Upper Miocene (9–10 Ma) of central Kenya. The new taxon possesses a unique combination of characters, including six lateral line foramina on the lacrimal, three lateral line segments, cycloid scales, and a low number of vertebrae (29), dorsal fin spines (13), and dorsal soft rays (9). Its lacrimal morphology and tripartite lateral line suggest an affinity with the present-day Lake Tanganyika tribes Ectodini and Limnochromini, and thus with members of the ‘East African Radiation’ among the African cichlids. To further elucidate the relationships of †T. pickfordi, we used a comprehensive comparative data set comprising meristic data from all present-day tribes of the ‘East African Radiation.’ Principal coordinates analyses support links between the fossil and Ectodini + Limnochromini, and additionally with modern Haplochromini. We conclude that †T. pickfordi could be an extinct lineage within the ‘most ancient Tanganyika tribes,’ or a stem lineage of the ‘ancient Tanganyika mouthbrooders.’ A direct relationship to the Haplochromini is unlikely because its members do not exhibit the derived characteristics of the lacrimal as seen in †T. pickfordi. Because Lake Tanganyika is located in the western branch of the East African Rift System, †T. pickfordi from the eastern branch supports the ‘melting-pot Tanganyika hypothesis,’ which posits that the cichlids of modern Lake Tanganyika are derived from riverine lineages that had already diversified prior to the lake formation. http://zoobank.org/urn:lsid:zoobank.org:pub:37520070-99B3-463C-B8E6-9452D1F62722 SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP. Citation for this article: Altner, M., U. K. Schliewen, S. B. R. Penk, and B. Reichenbacher. 2017 . †Tugenchromis pickfordi, gen. et sp. nov., from the upper Miocene—a stem-group cichlid of the ‘East African Radiation’. Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1297819.

Who's your mama? Riverine hybridisation of threatened freshwater Trout Cod and Murray Cod.

Rates of hybridization and introgression are increasing dramatically worldwide because of translocations, restocking of organisms and habitat modifications; thus, determining whether hybridization is occuring after reintroducing extirpated congeneric species is commensurately important for conservation. Restocking programs are sometimes criticized because of the genetic consequences of hatchery-bred fish breeding with wild populations. These concerns are important to conservation restocking programs, including those from the Australian freshwater fish family, Percichthyidae. Two of the better known Australian Percichthyidae are the Murray Cod, Maccullochella peelii and Trout Cod, Maccullochella macquariensis which were formerly widespread over the Murray Darling Basin. In much of the Murrumbidgee River, Trout Cod and Murray Cod were sympatric until the late 1970s when Trout Cod were extirpated. Here we use genetic single nucleotide polymorphism (SNP) data together with mitochondrial sequences to examine hybridization and introgression between Murray Cod and Trout Cod in the upper Murrumbidgee River and consider implications for restocking programs. We have confirmed restocked riverine Trout Cod reproducing, but only as inter-specific matings, in the wild. We detected hybrid Trout Cod–Murray Cod in the Upper Murrumbidgee, recording the first hybrid larvae in the wild. Although hybrid larvae, juveniles and adults have been recorded in hatcheries and impoundments, and hybrid adults have been recorded in rivers previously, this is the first time fertile F1 have been recorded in a wild riverine population. The F1 backcrosses with Murray cod have also been found to be fertile. All backcrosses noted were with pure Murray Cod. Such introgression has not been recorded previously in these two species, and the imbalance in hybridization direction may have important implications for restocking programs.

A Ploidy Difference Represents an Impassable Barrier for Hybridisation in Animals. Is There an Exception among Botiid Loaches (Teleostei: Botiidae)?

One of the most efficient mechanisms to keep animal lineages separate is a difference in ploidy level (number of whole genome copies), since hybrid offspring from parents with different ploidy level are functionally sterile. In the freshwater fish family Botiidae, ploidy difference has been held responsible for the separation of its two subfamilies, the evolutionary tetraploid Botiinae and the diploid Leptobotiinae. Diploid and tetraploid species coexist in the upper Yangtze, the Pearl River and the Red River basins in China. Interestingly, the species ‘Botia’ zebra from the Pearl River basin combines a number of morphological characters that otherwise are found in the diploid genus Leptobotia with morphological characters of the tetraploid genus Sinibotia, therefore the aim of the present study is to test weather ‘B.’ zebra is the result of a hybridisation event between species from different subfamilies with different ploidy level. A closer morphological examination indeed demonstrates a high similarity of ‘B.’ zebra to two co-occurring species, the diploid Leptobotia guilinensis and the tetraploid Sinibotia pulchra. These two species thus could have been the potential parental species in case of a hybrid origin of ‘B.’ zebra. The morphologic analysis further reveals that ‘B.’ zebra bears even the diagnostic characters of the genera Leptobotia (Leptobotiinae) and Sinibotia (Botiinae). In contrast, a comparison of six allozyme loci between ‘B.’ zebra, L. guilinensis and S. pulchra showed only similarities between ‘B.’ zebra and S. pulchra, not between ‘B.’ zebra and L. guilinensis. Six specimens of ‘B.’ zebra that were cytogenetically analysed were tetraploid with 4n = 100. The composition of the karyotype (18% metacentric, 18% submetacentric, 36% subtelocentric and 28% acrocentric chromosomes) differs from those of L. guilinensis (12%, 24%, 20% and 44%) and S. pulchra (20%, 26%, 28% and 26%), and cannot be obtained by any combination of genomes from L. guilinensis and S. pulchra. Phylogenetic reconstructions based on sequence data of the mitochondrial cytochrome b gene and the nuclear RAG-1 gene invariably places ‘Botia’ zebra as sister species to S. pulchra, while L. guilinensis is only distantly related. The presented combination of genetic data demonstrates that ‘B.’ zebra is not the result of a hybridisation, but a species of tetraploid genus Sinibotia with a striking morphological evolution towards an enormous similarity with a co-occurring, but not directly related species. The complete lack of knowledge of the ecology of these species, their main predators or their ecological interactions hampers any conclusion regarding the evolutionary advantage of such adaptation.

Characterizing the transcriptome and molecular markers information for roach, Rutilus rutilus.

Rutilus rutilus (roach) is native to most of Europe and western Asia, and the Irtysh River basin in Sinkiang, northwest China is the marginal area of their natural distribution. The wide distribution and unique characteristic of this species makes it an ideal model for analysing ecological and comparative genomics. However, the limited genome sequences available for this species have hindered these investigations. Transcriptomes from the brains and livers of five individuals collected from the Irtysh River basin were sequenced using Illumina paired-end sequencing technology. A collection of 132,289 unigenes for this species were obtained using a de novo assembly method based on nearly 120 million clean reads encompassing more than 14 Gb data. Approximately 37.5% (49,656), 27.1% (35,867) and 21.2% (27,987) of the transcriptome had homologues deposited in Nt, Nr and Swiss-Prot, respectively; 12.3% (16,328) were assigned to eukaryotic orthologous groups of proteins classifications, and 21.5% (28,429) harboured Interpro domains. On the basis of the assembled transcriptome, we detected 177,493 single-nuc leotide variation resident in 39.3% (52,029) of the sequences and 20.8% (27,497) of the sequences harbouring 36,639 simple sequence repeats. The identified molecular markers are a basis for further ecological analysis, and the transcriptome reported here allows for more extensive evolutionary analyses of the Cyprinidae, the most species-rich family of freshwater fishes.

Molecular Evidence for Multiple Origins of the European Spined Loaches (Teleostei, Cobitidae)

We present a phylogenetic investigation of the Northern Clade, the major monophyletic clade within the freshwater fish family Cobitidae, one of the most prominent families of freshwater fishes found in Asian and European waters. Phylogenetic reconstructions based on the cytochrome b and RAG-1 genes show the genera Microcobitis, Sabanejewia, Koreocobitis and Kichulchoia as monophyletic groups. These reconstructions also show a Cobitis sensu lato and a Misgurnus sensu lato group. The Cobitis sensu lato group includes all species of Cobitis, Iksookimia, Niwaella and Kichulchoia, while the Misgurnus sensu lato group includes Misgurnus, Paramisgurnus and Koreocobitis. Although the monophyly of both the Cobitis sensu lato and Misgurnus sensu lato groups is supported, relationships within the groups are incongruent with current generic definitions. The absence of monophyly of most genera included in the Cobitis sensu lato group (Cobitis, Iksookimia and Niwaella) or their low genetic differentiation (Kichuchoia) supports their consideration as synonyms of Cobitis. Molecular phylogenies indicate that the Asian species of Misgurnus experienced a mitochondrial introgression from a lineage of Cobitis. We also find two nuclear haplotypes in the same Cobitis species from the Adriatic area that, in the absence of morphological differentiation, may indicate molecular introgression. Most lineages within the Northern Clade consist of species found in East Asia. However, some lineages also contain species from Europe and Asia Minor. The phylogenetic relationships presented here are consistent with previous studies suggesting an East Asian origin of the Northern Clade. According to the current distributions and phylogenetic relationships of the Misgurnus sensu lato and Cobitis clade lineages, particularly of M. fossilis and C. melanoleuca, the range expansion of East Asian species into Europe was most likely via Siberia into Northern and Central Europe. Phylogenetic analyses also show that the Cobitis sensu lato group consists of two clear subgroups (I and II), each presenting geographical differences. Subgroup I is distributed exclusively in East Asian drainages with an Eastern European offshoot (C. melanoleuca), whereas Subgroup II includes species widespread throughout Europe (including the Mediterranean), Asia Minor, the Black Sea and the Caucasus, with some lineages related to species restricted to East Asia.

Host specificity and the structure of helminth parasite communities of fishes in a Neotropical river in Mexico.

In a tropical locality of Río La Antigua, Veracruz, Mexico, 11 fish species, represented by 244 individual fish from six freshwater fish families living sympatrically and synchronically, were examined for helminth parasites. A total of 36 taxa of helminths were recorded, 24 autogenic and 12 allogenic forms, including 6 monogeneans, 14 trematodes, 1 cestode, and 15 nematodes. Most helminth taxa were recovered for 10/11 of the component communities we analyzed. The results contribute empirical evidence that host specificity is an important force in the development of helminth communities of freshwater fishes. Each fish family has their own set of parasites, host species belonging to the same taxon share parasite species. High component community similarity among related host species was recorded, demonstrated by high prevalence and abundance, as well as dominance, of autogenic specialist species in each component community. Most autogenic helminth species are numerically and reproductively successful in relatively few host species. Autogenic helminths common in one host species are not common in others. Our findings give empirical support to the idea that low levels of sharing of parasites favor animal coexistence and high species richness, because large phylogenetic differences allow potentially competing animals to consume the same resources without being sensitive of another’s parasites.

Innate immune responses to gut microbiota differ between oceanic and freshwater threespine stickleback populations.

ABSTRACTAnimal hosts must co-exist with beneficial microbes while simultaneously being able to mount rapid, non-specific, innate immune responses to pathogenic microbes. How this balance is achieved is not fully understood, and disruption of this relationship can lead to disease. Excessive inflammatory responses to resident microbes are characteristic of certain gastrointestinal pathologies such as inflammatory bowel disease (IBD). The immune dysregulation of IBD has complex genetic underpinnings that cannot be fully recapitulated with single-gene-knockout models. A deeper understanding of the genetic regulation of innate immune responses to resident microbes requires the ability to measure immune responses in the presence and absence of the microbiota using vertebrate models with complex genetic variation. Here, we describe a new gnotobiotic vertebrate model to explore the natural genetic variation that contributes to differences in innate immune responses to microbiota. Threespine stickleback, Gasterosteus aculeatus, has been used to study the developmental genetics of complex traits during the repeated evolution from ancestral oceanic to derived freshwater forms. We established methods to rear germ-free stickleback larvae and gnotobiotic animals monoassociated with single bacterial isolates. We characterized the innate immune response of these fish to resident gut microbes by quantifying the neutrophil cells in conventionally reared monoassociated or germ-free stickleback from both oceanic and freshwater populations grown in a common intermediate salinity environment. We found that oceanic and freshwater fish in the wild and in the laboratory share many intestinal microbial community members. However, oceanic fish mount a strong immune response to residential microbiota, whereas freshwater fish frequently do not. A strong innate immune response was uniformly observed across oceanic families, but this response varied among families of freshwater fish. The gnotobiotic stickleback model that we have developed therefore provides a platform for future studies mapping the natural genetic basis of the variation in immune response to microbes.

Interspecific dietary diversity has little influence on pathways of glucose metabolism in liver and heart of piranhas and pacus (family Serrasalmidae)

The Serrasalmidae is a speciose family of Neotropical freshwater fishes with diverse interspecies dietary preferences, from the herbivorous or frugivorous pacus to the omnivorous or carnivorous piranhas. The Serrasalmidae is an ideal comparative model to explore the biochemical correlates of dietary preference. For example, debate exists about whether variation in dietary preference among fishes influences the capacities for de novo glucose synthesis (gluconeogenesis) or glucose use as metabolic fuel (glycolysis). We predicted that carnivory in piranhas would be associated with an enhanced gluconeogenic capacity compared with the herbivorous pacus, because carnivores consume less carbohydrate. As a corollary, we expected similar glycolytic capacities at a major site of glucose use (heart) in fishes with different diets. We measured activities of key enzymes of gluconeogenesis and the opposing reactions of glycolysis in liver (the primary gluconeogenic site) and heart of five serrasalmid species showing an interspecies dietary gradient from herbivory to omnivory to carnivory. The interspecific variation in enzyme activities was, in general, unrelated to dietary preference. Glycolytic capacity in heart was lower in herbivores but this may be related to non-diet factors. Among serrasalmid fishes, the enzymatic capacities of glucose metabolism are not overtly influenced by interspecific divergence of dietary preference.

Checklist of the helminth parasites of the genus Profundulus Hubbs, 1924 (Cyprinodontiformes, Profundulidae), an endemic family of freshwater fishes in Middle-America.

From December 2012 to November 2014, 267 fish belonging to the family Profundulidae (representing nine of the 11 species of the genus Profundulus) were collected in 26 localities of Middle-America, across southern Mexico, Guatemala, and Honduras, comprising the distribution range of the genus, and analyzed for helminth parasites. Additionally, a database with all ten available published accounts of the helminth parasite fauna of this genus (the only genus within the family) was assembled. Based on both sources of information, a checklist containing all the records was compiled as a tool to address future questions in the areas of evolutionary biology, biogeography, ecology and phylogeography of this host-parasite association. The helminth parasite fauna of this fish group consists of 20 nominal species, classified in 17 genera and 14 families. It includes six species of adult digeneans, five metacercariae, two monogeneans, one adult cestode, three adult nematodes and three larval nematodes. The profundulid fishes are parasitized by a specialized group of helminth species (e.g. Paracreptotrema blancoi sensu Salgado-Maldonado et al. (2011b), Paracreptotrema profundulusi Salgado-Maldonado, Caspeta-Mandujano & Martínez Ramírez, 2011, Phyllodistomum spinopapillatum Pérez-Ponce de León, Pinacho-Pinacho, Mendoza-Garfias & García-Varela, 2015, Spinitectus humbertoi Mandujano-Caspeta & Moravec, 2000, Spinitectus mariaisabelae Caspeta-Mandujano Cabañas-Carranza & Salgado-Maldonado, 2007 and Rhabdochona salgadoi Mandujano-Caspeta & Moravec, 2000), representing the core helminth fauna that are not shared with other Middle-American fish species.

Phylogenetic relationships and evolutionary history of the Mesoamerican endemic freshwater fish family Profundulidae (Cyprinodontiformes: Actinopterygii).

Freshwater fishes of Profundulidae, which until now was composed of two subgenera, represent one of the few extant fish families endemic to Mesoamerica. In this study we investigated the phylogenetic relationships and evolutionary history of the eight recognized extant species (from 37 populations) of Profundulidae using three mitochondrial and one nuclear gene markers (∼2.9 Kbp). We applied a Bayesian species delimitation method as a first approach to resolving speciation patterns within Profundulidae considering two different scenarios, eight-species and twelve-species models, obtained in a previous phylogenetic analysis. Based on our results, each of the two subgenera was resolved as monophyletic, with a remarkable molecular divergence of 24.5% for mtDNA and 7.8% for nDNA uncorrected p distances, and thus we propose that they correspond to separate genera. Moreover, we propose a conservative taxonomic hypothesis with five species within Profundulus and three within Tlaloc, although both eight-species and twelve-species models were highly supported by the bayesian species delimitation analysis, providing additional evidence of higher taxonomic diversity than currently recognized in this family. According to our divergence time estimates, the family originated during the Upper Oligocene 26 Mya, and Profundulus and Tlaloc diverged in the Upper Oligocene or Lower Miocene about 20 Mya.

Functional properties of hemoglobin during the adaptation of fish to low environmental pH

The dependence of the resistance of fish to acidification on the functional properties of hemoglobin has been studied in 21 species (nine families) of freshwater, marine, and anadromous fish. A direct correlation between the fish tolerance to low pH and the value of the Bohr effect has been revealed for the first time. On the basis of these data, the Bohr effect is proposed as a characteristic of the buffer properties of hemoglobin and acid resistance in fish. The curves of the oxygen equilibrium of hemoglobin are invariant in the fish having the lowest resistance to acidification (sturgeons). The maximum distortion of the invariance in the acidic buffer solution was observed in the most acid-resistant fish: pike and perch. The efficiency of the hemoglobin buffer system declined with decreasing resistance of fish to acidification.

Inferring phylogenetic structure and ancient hybridization within Salmonidae using next-generation sequencing

Event Abstract Back to Event Inferring phylogenetic structure and ancient hybridization within Salmonidae using next-generation sequencing Laurène A. Lecaudey1*, Steven J. Weiss1 and Ulrich K. Schliewen2 1 University of Graz, Institute of Zoology, Austria 2 Bavarian State Collection of Zoology, Department of Ichthyology, Germany Phylogenetic studies focusing on Salmonidae have thus far revealed significant drawbacks to elucidate some of the interspecific relationships within the family, due to limited number of markers, conflicting phylogenetic signals and hybridization events. In an attempt to resolve these issues, we applied restriction-site associated DNA sequencing (RAD-seq) to 44 samples, including 23 different species across the family Salmonidae, with an emphasis on the genus Salvelinus. The aim of our study is to infer reliable interspecific phylogenetic relationships, evaluate several putative scenarios of ancient hybridization and detect possible reticulate patterns of evolution among salmonid fishes. RAD-seq can be particularly useful for systematic studies of closely related taxa, as it provides genome-wide informative characters and allows the detection of introgression. To our knowledge, this is the first next generation sequencing effort applied to salmonid phylogeny and related questions of their evolutionary history. Using the software pipeline pyRAD, we identified 28,402 orthologous loci, which were subsequently used in our downstream analyses. Phylogenetic inferences drawn from a maximum likelihood analysis reveal some interesting results. Salvelinus and Oncorhynchus appear as sister lineages, which contradicts some of the previous phylogenetic analyses. Additionally, despite constituting a separate genus, Salvethymus clusters within the genus Salvelinus, which is consistent with the findings of previous studies and therefore questions its taxonomic status. A monophylum of Salvelinus leucomaenis and Salvelinus levanidovi is the sister group to the remainder of the genus Salvelinus. The genus Parahucho appears as the sister clade to Salmo, which also contradicts some previously published inferences. Finally, we present a practical application of a relatively novel approach to test for putative ancient hybridization events. Since this is the first Salmonidae phylogeny based on RAD-seq data, our findings represent a valuable contribution to the systematics of this important family of freshwater fishes. Our results provide a better understanding of the mechanisms of speciation and ecological adaptation of Salmonidae, as well as the effect of hybridization on speciation, within an evolutionary framework. Acknowledgements Financial support was provided by the Austrian Center for Limnology (ACL) at the University of Graz. Keywords: RAD-seq, NGS, Salmo, Salvelinus, Parahucho, pyRAD, Introgression Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Oral Presentation Topic: Phylogeny, Systematics and Genetics Citation: Lecaudey LA, Weiss SJ and Schliewen UK (2015). Inferring phylogenetic structure and ancient hybridization within Salmonidae using next-generation sequencing. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.fmars.2015.03.00008 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Oct 2015; Published Online: 04 Nov 2015. * Correspondence: Ms. Laurène A Lecaudey, University of Graz, Institute of Zoology, Graz, 8010, Austria, laurenelecaudey@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Laurène A Lecaudey Steven J Weiss Ulrich K Schliewen Google Laurène A Lecaudey Steven J Weiss Ulrich K Schliewen Google Scholar Laurène A Lecaudey Steven J Weiss Ulrich K Schliewen PubMed Laurène A Lecaudey Steven J Weiss Ulrich K Schliewen Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Ploidy differences represent an impassable barrier for hybridization. Is there an exception among botiid loaches (Cypriniformes: Botiidae)?

Event Abstract Back to Event Ploidy differences represent an impassable barrier for hybridization. Is there an exception among botiid loaches (Cypriniformes: Botiidae)? Joerg Bohlen1*, Vendula Šlechtová1, Vlastimil Šlechta1, Vera Šlechtová1, Alexandr Sember1 and Petr Rab1 1 Institute of Animal Physiology and Genetics AS CR, v.v.i., Czechia One of the most efficient mechanisms to keep animal lineages separate is a difference in ploidy level, since hybrid offspring from parents with different ploidy level are generally sterile. In the freshwater fish family Botiidae, ploidy difference has been held responsible for the separation of its two subfamilies, the tetraploid Botiinae and the diploid Leptobotiinae. However, the species ‘Botia’ zebra combines a number of morphological characters of a genus of Leptobotiinae with morphological characters of a genus of Botiinae, giving rise to the idea that it is the result of a hybridisation event between two species from different subfamilies. We here present a closer morphological examination, an allozyme comparison between ‘Botia’ zebra and the two potential parental species, a cytogenetic analyses as well as phylogenetic reconstructions basing on the complete mitochondrial cytochrome b gene and on the nuclear RAG-1 gene. The results are discussed in a phylogenetic, geographic and ecologic context. Acknowledgements The study was supported by grant 13-37277 S of the Czech Science Foundation (GAČR). Keywords: freshwater fishes, parallel evolution, Chromosome number, mimicry, ecological niche Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Oral Presentation Topic: Phylogeny, Systematics and Genetics Citation: Bohlen J, Šlechtová V, Šlechta V, Šlechtová V, Sember A and Rab P (2015). Ploidy differences represent an impassable barrier for hybridization. Is there an exception among botiid loaches (Cypriniformes: Botiidae)?. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00217 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 09 Dec 2015; Published Online: 09 Dec 2015. * Correspondence: Dr. Joerg Bohlen, Institute of Animal Physiology and Genetics AS CR, v.v.i., Liběchov, 277 21, Czechia, joerg_bohlen@yahoo.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Joerg Bohlen Vendula Šlechtová Vlastimil Šlechta Vera Šlechtová Alexandr Sember Petr Rab Google Joerg Bohlen Vendula Šlechtová Vlastimil Šlechta Vera Šlechtová Alexandr Sember Petr Rab Google Scholar Joerg Bohlen Vendula Šlechtová Vlastimil Šlechta Vera Šlechtová Alexandr Sember Petr Rab PubMed Joerg Bohlen Vendula Šlechtová Vlastimil Šlechta Vera Šlechtová Alexandr Sember Petr Rab Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Mitochondrial genome of the Neotropical catfish Ageneiosus pardalis, Lütken 1874 (Siluriformes, Auchenipteridae)

Ageneiosus pardalis is a trans-Andean member of the Neotropical freshwater fish family Auchenipteridae (Siluriformes). In this work, the complete mitochondrial genome of A. pardalis was pyrosequenced by FLX 454 technology. The mitochondrial genome is 16,484 bp in length and encodes 13 proteins, 22 tRNAs and 2 ribosomal RNAs. Additionally, the synteny is conserved with others species of the Auchenipteridae family as well as other Siluriformes.

Evaluacion de la tasa de consumo de oxigeno de Colossoma macropomum en relacion al peso corporal y temperatura del agua

Colossoma macropomum is a species in the Amazon region pertaining to the greatest diversity of freshwater fish family Characidae. At the laboratory level, the rate of oxygen consumption of (CO) was determined in a routine metabolism at different body weights: 40, 60, 90, 140 and 250 g (expressed per unit of body mass) and water temperature of 21, 26 and 31°C. The experiment was design as a completely randomized factorial arrangement (5x3x3), using a respirometer of 20 L and a water recirculation pump. CO measurements were recorded every 5 min by using a digital oximeter. It was shown that the CO maintains interaction with body weight (P) and water temperature (T) (P < 0.05). The results indicate that CO remains directly related to water temperature and inversely with body weight of the organisms. The regression between independent variables and CO were adjusted to potential equations: CO = 2844.9 P -0.734 , CO = 2555.9 P -0.624 and CO = 1945.4 P -0.507 for 21, 26 y 31°C respectively; where P expressed in kg, yielding high coefficients of correlation and determination. The resulting equation of the multivariate regression analysis was: CO = 4.839 - (0.706* P) + (9.106* T). The results provide information to estimate the capacity in an aquaculture environment based upon the oxygen demand of C. macropomum, contributing to the development of aquaculture with greater technical support.

STUDI KEKERABATAN IKAN FAMILIA CYPRINIDAE YANG TERTANGKAP DI SUNGAI SERAYU KABUPATEN BANYUMAS

Cyprinidae is a family of freshwater fish that has quite a lot of valuable members and spread almost all over the world. The purpose of this study was to determine the number of fish of the family Cyprinidae in Serayu River and their relationships. This study used survey method with purposive random sampling. Research materials were members of Cyprinidae taken from the River Serayu Banyumas, which was the collection of Mrs. Dian Bhagawati. The sequence of this study included sample collection, sample preservation, sample observation in the laboratory, i.e., identification, determination of morphological characters and species name of fish and their relationships. Observed variables were morphological characters that included body shape, colors, fins specification, the total and standard length of the body, the shape of the lateral line, the shape of the caudal fin, the position of the mouth, body length, the height and width of the caudal peduncle. The data were then computed to get the coefficient of association among species (S), clustered using UPGMA Cluster Analysis as implemented in Numerical Taxonomy System (NTSYS) version 2.02i. The observed data were also descriptively analyzed based on phenogram obtained. The fish diversity recorded were eight species i.e., Puntius orphoides, Barbodes gonionotus, Osteochilus sp., Osteochilus vittatus, Osteochilus hasselti., Labiobarbus leptocheilus, Cyprinus carpio, and Rasbora sp.. The results showed that the closest relationship based on morphological characteristics was between Puntius orphoides and Rasbora sp. which had the greatest association coefficient of 0.8292. The smallest value of the association was followed by P. orphoides and Osteochilus sp. with the value of 0.3000. This study was expected to provide information of Cyprinid fish in Serayu River and their phenetic relationship.

Fish and ancient lakes in the Dead Sea Rift: The use of fish remains to reconstruct the ichthyofauna of paleo-Lake Hula

In this study we use fish remains recovered at the Acheulian site of Gesher Benot Ya'aqov, dated to 0.78Ma, to reconstruct for the first time the fish community of paleo-Lake Hula. From Area A at the site, we identified 13 species belonging to three of the five recent native families of freshwater fish: Cyprinidae (carps), Cichlidae (Tilapinii, St. Peter fish), and Clariidae (catfish). The identified taxa included species endemic to Lake Hula, Tristramella simonis intermedia (Cichlidae) and Mirogrex hulensis (Cyprinidae), demonstrating continuity in the fish community for the last 0.8million years. In addition, some of the species-specific bones exhibited different morphotypes that raise the possibility of the past existence of other endemic species of cyprinids and catfish in the lake, including the large molluskivores Luciobarbus sp. (formerly Barbus). The paleoenvironmental implications of the identified ichthyofauna for the complexity of the aquatic habitat of paleo-Lake Hula are discussed.

Intercontinental comparison of fish ecomorphology: null model tests of community assembly at the patch scale in rivers

Community assembly is affected by environmental filtering that restricts viable phenotypes and by species interactions that impose limits on interspecific trait similarity. Relative influences of these processes should vary according to habitat features and dispersal. Species dispersion within assemblage trait space also should vary in relation to species richness, strength of competition, and the spatiotemporal scale of analysis. We examined ecomorphological diversity of two freshwater fish families (Neotropical Cichlidae, Nearctic Centrarchidae) to test theories of local assembly from regional species pools and theories of species packing within mesohabitat patches. Cichlid and centrarchid assemblages were surveyed in four floodplain rivers (two in South America and two in North America) during low‐water periods when fish densities are highest. Surveys were conducted in four mesohabitat types (submerged wood, leaf litter, rocks, sand bank) within river channels and floodplain lakes. We measured 23 morphological traits associated with locomotion and feeding. Principal components analysis was performed on the species × traits matrix, and species axis scores were used to calculate species pairwise Euclidean distances and indices of dispersion within assemblage morphospace: mean nearest‐neighbor distance (indicating similarity), mean distance to centroid (assemblage morphospace size), and standard deviation of nearest‐neighbor distance (evenness of dispersion within assemblage morphospace). A null model was used to assess whether patterns were significantly nonrandom. When data for all mesohabitat types were combined for each river, species were significantly overdispersed and the assemblage morphospace was larger than predicted at random in every case. Analysis of assemblages within mesohabitat patches of different types revealed, in every case, significant overdispersion of species in morphospace indicative of limiting similarity. The total assemblage morphospace was greater than expected for tropical cichlids, but not for temperate centrarchids. Trends of species dispersion with assemblage morphospace in relation to species richness within mesohabitat patches were not consistent among or within river systems, possibly indicating that patches were already saturated with these perciform fishes. Interregional comparisons suggest an influence from both adaptive diversification and environmental filtering at broad spatial scales. At the scale of mesohabitat patches in lowland rivers, cichlid and centrarchid assemblages revealed patterns of trait complementarity that imply limiting similarity and strong influence of biotic interactions.