Family Fabaceae Research Articles

In Silico Analysis of Bioactive Compounds from Leaves of Abrus precatorius L. (Rosary Pea) Against Breast Cancer.

Introduction Breast cancer is one of the most common causes of cancer among women. Since the administration of chemotherapy drugs can cause several adverse effects, thus it leads to research on effective treatment from natural sources. Leaves of Abrus precatorius L., a member of the Fabaceae family,contain several medicinal properties. It has drawn interest as an anti-cancer agent since its leaves contain different phytochemicals that can cause apoptosis in a variety of cancer types. Methods A total of 97 compounds were identified from the ethyl acetate extract of A. precatorius leaves by gas chromatography/mass spectrometry (GC/MS) analysis. Of those, eight compounds were selected based on the percentage area above 2. Cheminformatics software such as Molinspiration (Molinspiration Cheminformatics free web services, Slovensky Grob, Slovakia) was used to predict the molecular properties and bioactivity. PASS software (NCSS LLC, Kaysville, Utah, United States) was used to predict the scores of anticancer properties such as antioxidant, anti-inflammatory, immunosuppressant, antineoplastic, and cytoprotective. Osiris Property Explorer software was used to determine pharmacokinetic profile and toxicity prediction, and molecular docking was performed to determine the binding affinity towards the receptors. Results Out of eight compounds, one was selected based on the scores of the above software, then docking studies were done by using AutoDock Vina 4.2.6 (Center for Computational Structural Biology, La Jolla, California, United States). The results were compared with the reference compound, 5-fluorouracil, and 1,4-dimethyl-4 pentenyl acetate was identified as the most promising active compound found in this study. It shows better binding affinity towards the progesterone receptor (-6.0)when compared to the reference compound. Conclusion Based on the results, it has been proved that 1,4-dimethyl-4 pentenyl acetate maybe used as an alternative for the management of breast cancer.

Characteristic Exploration of Family Fabaceae Leaf Trichomes

This research aims to explore the characteristics of trichomes from the Fabaceae family. This type of research is exploratory descriptive research. Direct exploration and laboratory observation methods were used in the research. This research was carried out from March 2022 to January 2023. This research was carried out at the UMM Malabar City Forest and Biology Laboratory. Observation of trichomes using SEM. The research data obtained were analyzed using descriptive statistics. The results of the research show that there are variations in the characteristics of Fabacea family trichomes in the Malabar forest of Malang City. There are two varieties of trichomes, there are three forms of trichomes, namely subulate, osteolate and globular, the highest average number is Bauhinia purpurea leaf trichomes and the fewest are Albizia chinensis and Senna siamea, the trichomes with the longest size are Delonix regia and the shortest are Erythrina trichomes variegata, the narrowest trichomes are A. chinensis and the widest are E. Variegata. The results of the research can be used by stakeholders to become a consideration for selecting plant species in city parks by using plants that can help improve air quality in addition to considering aesthetics.

Ethnobotanical survey of multipurpose, medicinal and wild edible plants in the upper Gibe watershed landscapes, West Shewa zone, Ethiopia

Plants provide multiple ecosystem services that can, directly and indirectly, benefit the local community and beyond. This study assessed the diversity of the multiple uses of medicinal plants (MPs) and wild edible plants (WEPs) as well as associated local knowledge in Bako Tibe District, Ethiopia. Based on the recommendation of local authorities, eight key informants were selected out of the general informants (48) at eight sites. Ethnobotanical data were quantitatively analyzed through the use of use values, pairwise comparisons, and reference and direct matrix rankings. In total, 74 species from 61 genera and 36 families were found to have multiple uses. The Fabaceae family had the highest number of species (11), followed by the Moraceae family (eight). Trees were represented by 31 species. Twenty species were used to treat 22 human and livestock ailments. The highest preference ranking was recorded for Ocimum lamiifolium for treating febrile illness. Leaves (55.2%) and fruits (13 species) were the most commonly used parts for MP and WEP species, respectively. The highest use value was recorded for Cordia africana, among the top 10 multipurpose species. The findings showed that the most preferred MP and WEP species need further phytochemical and food nutritional analysis, respectively.

Physiological Responses of Crotalaria spp. to the Presence of High Aluminum Availability in the Soil.

Brazilian soils are predominantly rich in aluminum, which becomes mobile at pH < 5, affecting sensitive plants; however, some species have developed aluminum tolerance mechanisms. The purpose of this study was to compare the physiological responses of Crotalaria genus species, family Fabaceae, which have the ability to associate with nitrogen-fixing bacteria under the influence of Al3+ in the soil. The soil used was Oxisol; the experimental design was in randomized blocks in a factorial scheme (2 × 3): soil factor (available toxic aluminum content; correction of dolomitic limestone-MgCO3) and species factor (C. juncea; C. spectabilis; C. ochroleuca); cultivated within 43, 53, and 53 days, respectively, with five replications; 30 experimental samples. Mass and length, pigments, gas exchange, and changes in nitrogen metabolism were evaluated. C. juncea showed a higher concentration of amino acids in the leaves, internal carbon, and stomatal conductance in soil with Al3+, as well as higher production of ureides, allantoinic acid, allantoic acid, proteins, and amino acids in the nodules, with 78% of the Al3+ accumulation occurring in the roots. C. ochroleuca demonstrated greater shoot length and nodule number production in limed soil; in soil with Al3+, it showed a 91% increase in chlorophyll a content and 93% in carotenoids. C. spectabilis showed a 93% increase in ureide production in the leaves in soil with Al3+.

Entomophilous Pollen and Bees as Biomonitors and Bioindicators of the Anthropogenic Triad

The progressive increase in the concentration of harmful pollutants in the environment threatens human health. To record them, researchers use biomonitors; plant pollen and honeybees (Apis mellifera) have proven themselves well in this capacity. The peculiarity of bees is that while searching for food, they collect pollutants from the environment: air, soil and water within a radius of no more than 3 km. Not only plant pollen, but also honeybees can act as bioindicators of the anthropogenic triad: climate change and traditional land use systems and environmental pollution. In various search engines, Internet users are interested in these issues, because their point of refraction is beekeeping products, including honey. It includes almost the entire spectrum of allergenic pollen, starting from wormwood pollen — the regional allergen of the republic and ending with pollen of trees and shrubs. The problem of allergies to ornamental plants is still poorly understood. Reports of occupational flower allergies are few and usually involve gardeners, greenhouse workers, landscapers, and florists. Handling, smelling and caring for flowers can cause rhino conjunctivitis, asthma, urticaria, and contact dermatitis. When studying the pollen spectrum in the air of cities, entomophilous pollen grains of the families Malvaceae, Fabaceae, Asteraceae, and Rosaceae were identified. In the modern world, the concept of “One Health” has gained popularity, which recognizes the interconnectedness of the health of people, animals and ecosystems, therefore it is accepted both at the national and global levels. Within its framework, we are forming a new concept: “Entomophilous pollen and bees as biomonitors and bioindicators of the anthropogenic triad: climate change and land use systems; environmental pollution”. The purpose of the presented article is to systematize the results obtained and literature sources within the framework of the formulated concept.

EFFECT OF HERBIVORY ON THE REFORESTATION OF FOUR SPECIES OF THE FABACEAE FAMILY IN DETERIORATED AREAS

Reforestation plays an important role in attempting to reverse ecosystem degradation in arid regions, where natural rainfall variability makes ecological restoration difficult. The aim of this work was to evaluate the survival and growth of four native species of the Fabaceae family (Prosopis velutina Wooton, Havardia mexicana (Rose) Britton &amp; Rose, Parkinsonia microphylla Torr., and Olneya tesota A. Gray), with and without protection against herbivory in a degraded arid land in Sonora, Mexico. A three-level factorial design was established according to the protection treatments (PET and mesh, 20 and 30 cm in height, respectively) and a control (without protection). The variables of survival and height were evaluated 6, 12, and 24 months after transplant. Computer packages were used for the statistical analyses, establishing 5 % significance. Survival was highest in the plants with protection, with 100 % for the first year, both for PET and mesh, in comparison with a survival rate of 16.6 % for the unprotected control plants. The second year, the highest survival rate was found in plants protected by a mesh (41.6 %), whereas plants protected with PET and the control had the lowest survival rates, both with 8.3 % (χ2 = 5.674, p ≤ 0.058). The height of plants was affected by herbivory. Plants protected by mesh reached a greater height (29.4 cm) in comparison with the PET treatment, with 17.4 cm, and the control, with 3.1 cm (p &lt; 0.0001). The growth of native forage fabaceae (leguminous) tree species in Sonora is extremely slow and relies on both herbivore protection and above-average rainfall years. This work presents the use of low-cost herbivory protection strategies to increase the survival and height of plants 2 years after being established.

Impacts of cyanobacteria-dominated biological soil crusts on seedling emergence and fate: an in situ experiment

Abstract Background and aims Biological soil crusts (BSCs) co-occur with vascular plants in many ecosystems. Nevertheless, little is known regarding the interaction among BSCs and vascular plants, and no standard methods exist aiming to evaluate BSC–plant interactions. While some reports emphasized the inhibition effect of BSCs on germination, others reported the opposite or did not find any differences between germination under crusted and non-crusted conditions. Contradictory findings were also reported regarding the BSC effects on vascular plants after germination, with some reports claiming that BSCs promote growth, while others claim the opposite. Methods By a seed addition experiment (using light and heavy seeds of three species belonging to the Fabaceae family) in an early-successional system in Germany, we tested in situ the emergence of seedlings under variable surface conditions (cyanobacterial crust, disturbed and removed crust), and quantified crust-plant interactions (survival, flowering) during and following seedling emergence. Results In comparison to an average of 8% of the light Lotus corniculatus and 20% of the light Ornithopus sativus that emerged through the cyanobacterial BSC, 76% of the heavy Glycine max emerged. Once emerged, the crust did not significantly affect the survival, growth, biomass, or flowering of G. max, whereas many of the seedlings from lightweight seeds dried up soon after emergence. The thickness of the cyanobacterial biocrust had no influence on Fabaceae emergence. Conclusion The cyanobacterial BSC acted as a filter that hindered the emergence of the lightweight seeds.

Nature’s Precision: The Intricate Pollen Dispersal Mechanisms of Papilionaceous Crops

Pollination, a fascinating natural process, is crucial for the reproduction of flowering plants. It involves the transfer of pollen from the male reproductive part to the female reproductive part, enabling successful fertilization. Papilionaceous crops, belonging to the Fabaceae family, exhibit highly specialized pollen dispersal mechanisms that ensure effective pollination and genetic diversity. These mechanisms involve intricate interactions between floral structures and pollinators, primarily bees. By integrating findings from various studies, this review provides a comprehensive understanding of the intricate pollen dispersal mechanisms in papilionaceous crops such as piston/pump mechanism, explosive type mechanism, brush type mechanism and valvular mechanism. It also provides information about the role of different floral parts in pollen dispersal among the flowers. This review will be helpful in gaining insights about pollen dispersal mechanism and it contribution in improving crop yields and breeding programs.

The complete chloroplast genome of Flemingia stricta Roxb. ex Ait. 1812 (Phaseoleae, Fabaceae)

Flemingia stricta Roxb. ex Ait. 1812 belongs to the Phaseoleae tribe within the Fabaceae family and has significant pharmaceutical value. In this study, we reported the complete chloroplast genome of F. stricta using the Illumina DNA sequencing data. The chloroplast genome was 152,940 bp and encoded 111 unique genes, including 77 protein-coding genes (PCGs), 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. The phylogenetic analysis confirmed that F. stricta was closely related to Flemingia prostrata and Flemingia macrophylla. The chloroplast genome of F. stricta could provide critical information for the molecular breeding of F. stricta and be used as a reference genome for other species of Phaseoleae.

Ethnobotanical study of the traditional use of medicinal plants used for treating human diseases in selected districts of West Gojjam zone, Amhara Region, Ethiopia

Introduction: Historically, Ethiopians have relied on medicinal plants to heal a wide range of human diseases. However, indigenous knowledge associated with medicinal plants has been lost due to the secrecy of the knowledge transfer system and several anthropogenic factors. Thus, this study was aimed to document ethnobotanical study of medicinal plants in three districts (Sekela, Dembecha, and South Achefer) of west Gojjam zone of the Amhara Regional State.Methods: Ethnobotanical data were collected between November 28, 2021 to December 22, 2022 using a semi-structured questionnaire, a focus group discussion, and field observations. Data were gathered from 30 key informants and 399 general informants. Key informants and general informants were selected by purposively and random sampling method, respectively. Descriptive statistics and ranking analyzing methods was used to analyze the data. During the process of data analysis, we pooled data from three districts into a single dataset. Combining data from three districts into one analysis allows for a broader understanding of the traditional knowledge and practices within the districts.Results: A total of 97 medicinal plants were identified, with the Fabaceae family being the most prominent, followed by the Asteraceae. Herbs were the dominant plant habit, and leaves were the most frequently used plant part for medicines. The most common method for preparing remedies was crushing, and oral delivery was the primary administration method. Olea europaea was identified as the most multipurpose plant species, particularly effective under high pressure, with an informant consensus factor of 0.92 for dermatological diseases. Calpurnia aurea was the top ranked for treating diarrhea, followed by Rumex nepalensis. The decline of medicinal plants in the study areas was primarily due to charcoal production and agricultural activities, leading herbalists to collect remedies from forests and remote locations.Conclusion: This study underscores the vital role of indigenous knowledge and biodiversity by documenting medicinal plant use in Sekela, Dembecha, and South Achefer districts. Urgent conservation measures are imperative to safeguard these plants and sustain traditional medicinal practices.

A systematic review exploring the diversity and food security potential of wild edible plants in Ethiopia

Wild edible plants (WEPs) are important food sources globally due to their accessibility and affordability. In Ethiopia, where diverse cultural groups consume WEPs, this systematic review explores their diversity, edible parts, and role in supporting food security. The review examined 38 original studies on the ethnobotany of WEPs in Ethiopia from 2000 to 2022. It identified a total of 651 WEP species from 343 genera and 94 families, with the Fabaceae family having the most species (51). Herbs and shrubs were the predominant growth habits, and fruits were the most consumed plant parts. The review prioritized nine WEP species for cultivation and promotion. However, threats such as overgrazing, agricultural expansion, and the use of woody species for construction, firewood, and charcoal have depleted WEP resources and eroded traditional knowledge about their use. The review suggests that WEPs have the potential to contribute to food and nutritional security in Ethiopia if these threats are effectively managed. However, the limited coverage of ethnobotanical studies on WEPs requires further investigation. The study recommends integrating the prioritized WEPs into the national food system for promotion, cultivation, and nutrient analysis to evaluate their nutritional bioavailability.

Cultural Use and the Knowledge of Ethnomedicinal Plants in the Pülümür (Dersim-Tunceli) Region.

Pülümür has been a refuge place for internal and external exiles several times throughout history, and today it is a district of the province of Tunceli (known as Dersim in the region), which differs significantly from many settlements around it in terms of biodiversity and social aspects. The aim of this study is to identify, catalog, and report the traditional medicinal uses of plants in this province, where every living thing and many natural objects around it are regarded as sacred, with a scientific ethnobotanical approach. The field study was completed between May 2016 and July 2023. The collection of plant excursion and plant usage information was implemented in two stages; in the first stage, a survey about the collection and the uses of medicinal plants was conducted. At this stage, plant samples were collected by visiting 49 villages of the district and performing face-to-face interviews with 112 participants (51 female and 61 male). In the second stage, the usage statistics related to the data obtained from the field studies were determined. For this reason, every informant was interviewed at least twice, people who were previously interviewed were included too. In the course of this study, interviews from 211 participants (95 female and 116 male) were analyzed. As a result of analysis, the traditional medicinal uses of 120 plants belonging to 48 families were identified. The most taxa are identified from the Asteraceae (15), Rosaceae (13), Fabaceae (8), Lamiaceae (8), and Apiaceae (6) families. In Pülümür, these medicinal plants are mostly used for the treatment of wounds, abdominal pain, constipation, and diabetes.

New records for the Middle Siwalik flora of eastern Nepal and their climatic significance

The Siwalik plant fossils are important in understanding the climate change caused by the uplift of the Himalaya. The plant fossil assemblages are abundantly known from Nepal's central and western Siwalik sediments. However, they are poorly known from the Siwalik of the eastern region from where the modern Indian Summer Monsoon (ISM) enters Nepal. That is why fossils of this region are important in understanding the evolutionary history of ISM in Nepal. In the present communication, we report four new fossil leaf impressions/ compressions, namely Thelypteridaceophyllum tertiarum Joshi and Mehrotra of the family Thelypteridaceae, Calophyllum suraikholaensis Awasthi and Prasad of the Calophyllaceae, Albizia siwalica Prasad of the family Fabaceae, and Daphnogene makumensis Mehrotra, Dilcher et Lott of the family Lauraceae from the well-dated Middle Siwalik (∼9.5 Ma) sediments of eastern Nepal. The aforementioned and previous fossil records suggest that seasonal or monsoonal rainfall was present during the deposition of the sediments.

Characterizing Dry Pea (Pisum sativum L.) for Improved Nutritional Traits and the Potential for Biofortification

ABSTRACTDry pea (Pisum sativum L.) is a highly nutritious cool season food legume or pulse crop within the Fabaceae family that features high levels of protein (20%–25%), prebiotic carbohydrates, and a range of minerals and vitamins. Dry pea is cultivated globally in temperate climates and consumed as a whole food, snack, or protein powder. Dry pea is featured in plant‐based meat items such as the “beyond” branded plant‐based meats. Dry pea is an excellent candidate for plant‐based protein alternatives due to the high protein and low‐fat concentrations present in the mature seed, but improvements are still needed for more widespread use. Breeding efforts are ongoing to further improve dry pea proteins' quality, quantity, and digestibility through biofortification. Global dry pea germplasm contains a wide array of accessions that are vital for dry pea breeding efforts focused on developing cultivars enriched with the most bioavailable forms of plant‐based proteins. The objective of this review is to summarize prior research exploring the factors that contribute to the nutritional value of the dry pea—especially protein quality and quantity.

Phytochemical and Phrmacological Review on Butea Monosperma (PALASH)

There are many natural crude drugs that have ability to treat many diseases and disorder, one of them is Butea Monosperma. It belongs to the plant family Fabaceae. Popularly known as ‘dhak’ or Palash, ‘Flame of forest’ due to bright orange and Scarlet colour of its flowers. The trade name ‘Butea’ of which has taken from its scientific name Butea Monosperma native to tropical subtropical part of India, ranging across India, Sri Lanka, Bangladesh, Nepal, Myanmar, Thailand etc. It has been used for the treatment of different ailments such as cancer, diabetes, diarrhea, dysentery, fever, jaundice. many investigation and studies found it's antioxidant, antidiabetic, antiviral, anticancer properties. It contains butrin, isobutrin, triterene, coreserpine, iscoresprpine, isomonospermaoside, flavonoids, steroids. The present review discusses the morphology, etanobotany, phytochemical constituents, traditional uses, pharmacological activities of plants in details

Study on ethnoveterinary medicinal plants used by local people inhabiting char area, Kamrup District, Assam, North-East India

The inhabitants of char areas of Kamrup district, Assam (India) have relied on medicinal plants to treat various livestock diseases. Due to the lack of proper documentation, these plants have received little attention. Therefore, studying these plant species' diversity, utilisation, and phytochemistry is crucial. By doing so, we can preserve their traditional knowledge and comprehend the possibility of using these plants to develop new drugs. The present investigation documented 30 medicinal plant species belonging to 29 genera and 22 families. These plants were used against the treatment of 12 disease categories. The families Fabaceae and Zingiberaceae, with three species for each, were dominant, while the leaf (22%) and seed (16%) were the most frequently used plant parts. The informant consensus factor (Fic) was found to be highest in neurological disorders and neglected tropical diseases (NTD) with a value of 1.0 each. In contrast, the relative frequency of citation (RFC) was found to be highest in Curcuma longa L. (0.41) and Zingiber officinale Roscoe (0.37). This study provides reliable data on medicinal plants and indigenous ethnoveterinary knowledge that could act as a reference for future researchers dealing with new drug discovery.

The influence of different methods of under-vine management on the structure of vegetation and the qualitative parameters of the grapes in the Moravian wine region

ABSTRACT The present article explores the importance of vineyard soil management. Studies on under-vine management have yielded a wide range of results, with some indicating potential benefits for vine growth and productivity. However, the methods of under-vine management and their specific effects on vineyard parameters require further research. The aim of this study is to evaluate the relationships existing between the different types of management of under-vine areas and the vegetation grown in this zone and to determine the effects of under-vine management on the yield and quality of Traminer grapevines. By examining various approaches, the research aims to provide insights into the optimising of vineyard management methods to achieve enhanced biodiversity and grape quality in this specific geographical context. The findings indicated that management of the under-vine area can impact the composition of plants, grape yield and quality. Under-row management had an effect on the number of plant species and their composition. The highest weight of bunches was found in MIX (a species from the Fabaceae family), while the lowest was found in the MECH treatment (bare soil). MULCH, MONO and MIX consistently had higher yeast assimilable nitrogen (YAN) in grapes than other treatments. In many of the evaluated parameters, the influence of the seasons was evident.

Assessment of the current condition and ontogenetic structure of the populations of Leontice incerta Pall. (Berberidaceae) in the Kyzyl-Kum Desert, Uzbekistan

Abstract. Bobokandov N, Nomozova Z, Tashpulatov Y, Isomov E, Akhmedov A. 2024. Assessment of the current condition and ontogenetic structure of the populations of Leontice incerta Pall. (Berberidaceae) in the Kyzyl-Kum Desert, Uzbekistan. Biodiversitas 25: 2757-2764. The desert ecosystems of central Asia, including Uzbekistan, have a rich biodiversity and unique plant communities. High human pressure and long drought periods due to climate change have caused habitat destruction in these areas and a parallel crisis in vegetation cover. This study aimed to estimate the current population of Leontice incerta Pall., primarily distributed in the Kyzyl-Kum Desert, Uzbekistan. This focal species grew under climatic changes and human pressure in the Kyzyl-Kum Desert. The study identified five populations of L. incerta in the Kyzyl-Kum Desert. All 5 populations were estimated and measured by delta-omega and the population spectrum was determined. In the plant community, there are mostly dominated by Asteraceae (6 species), followed by Fabaceae (5 species), Amaranthaceae (4 species), Lamiaceae (4 species), Apiaceae (3 species), and other families. The plant communities consist of 49 species, including 1 species of tree, 6 species of shrubs, 6 species of semi-shrubs, 26 species of perennial herbs, and 10 species of annual herbs. The ontogenetic structure was incomplete, that is, it did not include all age groups due to biological features and harsh conditions. At all sites, the population density was low with most populations classified as young ontogenetic structures. The results will make it possible to understand estimating the current state of L. incerta. It indicated this species might, soon, become rare in the wild, therefore conservation and protection areas for this focal species are necessary.

Effects of Water and Nitrogen on Growth, Rhizosphere Environment, and Microbial Community of Sophora alopecuroides: Their Interrelationship.

The effective management of water and nitrogen is crucial in the artificial cultivation of medicinal plants. Sophora alopecuroides, a perennial herbaceous plant in the Fabaceae family, is extensively used in medicine, with alkaloids as its primary bioactive constituents. Nevertheless, there remains a significant knowledge gap regarding how rhizospheric microbial communities respond to varying water and nitrogen conditions and their intricate relationships with soil environments and the growth of S. alopecuroides. In this study, two-year-old S. alopecuroides were used in a two-factor, three-level water-nitrogen interaction experiment. The irrigation levels included W1 (30-35% of maximum water holding capacity), W2 (50-55%), and W3 (70-75%), while nitrogen levels comprised N1 (32 mg/kg), N2 (64 mg/kg), and N3 (128 mg/kg). The study assessed plant growth indicators, total alkaloid content, and rhizospheric soil physicochemical parameters of S. alopecuroides. High-throughput sequencing (16S rRNA and ITS) was employed to analyze variations in rhizospheric microbial community composition and structure. The results showed that Proteobacteria and Ascomycota are the predominant bacterial and fungal phyla in the rhizosphere microbial community of S. alopecuroides. The highest biomass and alkaloid accumulation of S. alopecuroides were observed under the N1W3 treatment (50% nitrogen application and 70-75% of maximum water holding capacity). Specifically, six bacterial genus-level biomarkers (TRA3_20, MND1, env_OPS_17, SBR1031, Haliangium, S0134_terrestrial_group) and six fungal genus-level biomarkers (Pseudeurotium, Rhizophagus, Patinella, Pseudeurotium, Patinella, Rhizophagus) were identified under the N1W3 treatment condition. In the partial least squares path modeling (PLS-PM), water and nitrogen treatments demonstrated markedly positive direct effects on soil physicochemical parameters (p < 0.01), while showing significant negative direct impacts on alkaloid accumulation and plant growth indicators (p < 0.05). Soil physicochemical parameters, in turn, significantly negatively affected the rhizosphere fungal community (p < 0.05). Additionally, the rhizosphere fungal community exhibited highly significant negative direct effects on both the plant growth indicators and total alkaloid content of S. alopecuroides (p < 0.01). This study provides new insights into the interactions among rhizosphere soil environment, rhizosphere microbiota, plant growth, and alkaloid accumulation under water and nitrogen regulation, offering a scientific basis for the water and nitrogen management in the cultivation of S. alopecuroides.

Diversity and use of Plants in Carlos Concha, Esmeraldas, Ecuador

In Carlos Concha, Esmeraldas province, a survey was conducted in order to determine the level of use of local plants by the local population. It was determined that 49 species, belonging to 28 botanical families are used. Most of the latter belong to the Asteraceae, Cucurbitaceae, Fabaceae, Piperaceae, and Solanaceae families, which include 17.85% of all reported species. All collected plants were collected in the field, pressed, dried, and mounted as herbarium samples. Most of the inhabitants of Carlos Concha practice subsistence agriculture with cacao (Teobrama cacao), maize (Zea mays), cassava (Manihot esculenta), plantains (Musa paradisiaca), and bananas (Musa acuminata) the principal crops. Of the plants reported as useful, 50.1% are used as medicine, insect repellent, and wound healing. As much as 24.4% of the reported species are used as food, three species are used as construction materials, whereas uses related to handicraft manufacturing and ritual activities are less frequent, with only one species used for these purposes. Additionally, 9 species were reported to be used for other purposes, including fuel (coal), forage and ornamental uses.