Diverse Family Research Articles

Investigating the impact of screen-sharing visual aids during genomic results disclosure via telehealth in diverse families in the TeleKidSeq pilot study.

Telehealth genetic counseling is comparable to in-person visits in terms of satisfaction, knowledge, and psychological outcomes, but using visual aids can be challenging on telehealth platforms. This pilot study assessed if the "screen-sharing" feature via Zoom to display visual aids during results disclosure session positively impacted parental experience and comprehension of their child's genomic results especially in underrepresented groups and those with limited English proficiency. In the TeleKidSeq pilot study, 409 children with suspected genetic conditions underwent genome sequencing. Families were randomized to receive genomic results via televisits with (ScrS) or without (NScrS) screen-sharing of visual aids. Spanish- or English-speaking parents/legal guardians completed surveys at three timepoints to assess perceived and objective understanding, perceived confidence, and telehealth experience. Regression models evaluated the effect of screen-sharing over time. Overall, understanding and telehealth experience ratings were high, with no significant differences between the ScrS (N = 192) and NScrS (N = 200) arms with regard to perceived (p = 0.32) or objective (0.94) understanding, confidence (p = 0.14) over time, or telehealth experience (p = 0.10). When stratifying by sociodemographic characteristics and type of device used during results disclosure, we observed subtle differences in the effect of screen-sharing within some sub-groups. While screen-sharing had no significant impact on overall outcomes, we identified modest effects of screen-sharing within population groups that highlight the need for tailored communication strategies to ensure diverse, multilingual communities derive equitable benefit from telehealth-based genomic results disclosure. Future research is needed to determine whether certain types of visual aids best enhance genomic results disclosure in larger, more robust studies designed to detect smaller effects and subgroup differences.

A Novel Screening System to Characterize and Engineer Quorum Quenching Lactonases.

N-acyl l-homoserine lactones are signaling molecules used by numerous bacteria in quorum sensing. Some bacteria encode lactonases, which can inactivate these signals. Lactonases were reported to inhibit quorum sensing-dependent phenotypes, including virulence and biofilm. As bacterial signaling is dependent on the type of molecule used, lactonases with high substrate specificity are desirable for selectively targeting species in communities. Lactonases characterized from nature show limited diversity in substrate preference, making their engineering appealing but complicated by the lack of convenient assays for evaluating lactonase activity. We present a medium-throughput lactonase screening system compatible with lysates that couples the ring opening of N-acyl l-homocysteine thiolactones with 5,5-dithio-bis-(2-nitrobenzoic acid) to generate a chromogenic signal. We show that this system is applicable to lactonases from diverse protein families and demonstrate its utility by screening mutant libraries of GcL lactonase from Parageobacillus caldoxylosilyticus. Kinetic characterization corroborated the screening results with thiolactonase and homoserine lactonase activity levels. This system identified GcL variants with altered specificity: up to 1900-fold lower activity for long-chain N-acyl l-homoserine lactone substrates and ~38-fold increase in preference for short-chain substrates. Overall, this new system substantially improves the evaluation of lactonase activity and will facilitate the identification and engineering of quorum quenching enzymes.

The infectivity of virus particles from Wolbachia-infected Drosophila

Viruses transmitted by arthropods pose a huge risk to human health. Wolbachia is an endosymbiotic bacterium that infects various arthropods and can block the viral replication cycle of several medically important viruses. As such, it has been successfully implemented in vector control strategies against mosquito-borne diseases, including Dengue virus. Whilst the mechanisms behind Wolbachia-mediated viral blocking are not fully characterised, it was recently shown that viruses grown in the presence of Wolbachia in some Dipteran cell cultures are less infectious than those grown in the absence of Wolbachia. Here, we investigate the breadth of this mechanism by determining if Wolbachia reduces infectivity in a different system at a different scale. To do this, we looked at Wolbachia’s impact on insect viruses from two diverse virus families within the whole organism Drosophila melanogaster. Drosophila C virus (DCV; Family Dicistroviridae) and Flock House virus (FHV; Famliy Nodaviridae) were grown in adult D. melanogaster flies with and without Wolbachia strain wMelPop. Measures of the physical characteristics, infectivity, pathogenicity, and replicative properties of progeny virus particles did not identify any impact of Wolbachia on either DCV or FHV. Therefore, there was no evidence that changes in infectivity contribute to Wolbachia-mediated viral blocking in this system. Overall, this is consistent with growing evidence that the mechanisms behind Wolbachia viral blocking are dependent on the unique tripartite interactions occurring between the host, the Wolbachia strain, and the infecting virus.

Predaceous and Phytophagous Pentatomidae Insects Exhibit Contrasting Susceptibilities to Imidacloprid.

Imidacloprid, a widely used neonicotinoid insecticide, targets insect pests but also affects natural enemies. However, the effects of neonicotinoid insecticides on closely related insects remain unclear. We evaluated the harmful effects of imidacloprid on the phytophagous Halyomorpha halys and predaceous Arma chinensis. Bioassays revealed that imidacloprid was more toxic to H. halys than to A. chinensis and more harmful to the males than to the females of the two insects. A. chinensis adults recovered from imidacloprid-induced knockdown, as evidenced by restored respiratory rates, metabolic rates, and locomotion. Surviving A. chinensis showed reduced fecundity, suggesting a trade-off between detoxification and reproduction. Bioinformatics analysis of nicotinic acetylcholine receptors (nAChRs) and molecular docking simulations indicated a lower diversity of the nAChR gene family in A. chinensis than in H. halys, with weaker binding to imidacloprid, consistent with the relatively low toxicity of the insecticide in this species. This might account for the susceptibility differences to imidacloprid between the species. These findings underscore the efficacy of imidacloprid against H. halys and provide insights into the toxicities of neonicotinoids to target and non-target insects.

The Impact of Family of Origin on Romantic Relationships: A Psychological Perspective

This review examines the influence of diverse family of origin factors on adult romantic relationships, concentrating on critical elements such as attachment styles, communication patterns, conflict resolution, family cohesion, family structure, and self-differentiation and self-regulation formed by early family experiences. Existing research reveals that early family dynamics significantly affect individuals' behaviours, beliefs, and satisfaction in romantic relationships during adulthood. The paper consolidates empirical findings from multiple studies, accentuating the role of secure and insecure attachment styles in determining romantic relationship satisfaction. It also investigates how family functions, specifically communication patterns, conflict management, and family cohesion, influence relationship outcomes. Additionally, the review highlights the degrees of self-differentiation and self-regulation influence the quality of intimate relationships. Psychological frameworks including attachment theory, family systems theory, and social learning theory offer the primary perspectives for comprehending these influences. The review also recognises critical deficiencies in the literature, including the requirement for more longitudinal studies and cross-cultural social class and gender comparisons. Overall, Individuals who grow up in nurturing, positive family environments with good parent-child interactions tend to have higher quality and more satisfying romantic relationships in adulthood. Studying on the impact of family of origin on romantic relationships can assist society in resolving family emotional conflicts and fostering harmony, it can likewise enrich the theoretical framework of family and emotional-related research in the academic field.

Factors associated with children’s attachment hierarchy in lesbian, gay, and heterosexual parent families through assisted reproduction

Abstract With few exceptions, previous studies of children’s identification and hierarchy of attachment figures have involved only children born to heterosexual parents through unassisted conception, recognizing mothers as the primary attachment figures. Further research into diverse families may help to disentangle parents’ attachment role from their gender, sexual orientation, and biological relatedness to children. The present study aimed at exploring whether differences in children’s choice of primary attachment figure could be explained by parents’ gender, biological status, attachment state of mind, reflective functioning, parenting quality, or by the child’s gender, across diverse family types. In total, 152 parents (i.e., 60 lesbian mothers through donor insemination, 50 gay fathers through surrogacy, 42 heterosexual parents through gamete donation) and their 76 school-aged children participated in the study. Children were interviewed about their choice of attachment figure in different situations. Parents completed questionnaires about parenting stress, parental rejection, parent–child relational efficacy, and parent positivity; and were administered the Adult Attachment Interview as a measure of their attachment state of mind and reflective functioning. Overall, no significant differences emerged between mothers and fathers or between biological and non-biological parents for children’s choice of primary attachment figure. However, fathers with higher RF were more likely to be identified as a primary attachment figure, and gay fathers who demonstrated less parental rejection were more likely to be chosen as a primary attachment figure. The findings provide important insights for social policies aimed at promoting preventive parenting interventions targeting the child–parent attachment bond in diverse families.

Penanaman Adab di Pesantren Tahfidzul Qur’an Sains Ar-Risalah

Adab of santri refers to the behavior exhibited by students in their daily lives, both within and outside the pesantren. Variations in personality are natural, as students come from diverse family and cultural backgrounds. One of the defining characteristics of a pesantren is its role as a place where students learn and develop into individuals with noble character and proper etiquette. The formation of a santri’s etiquette naturally involves gradual stages and habitual practices established through structured rules. Therefore, Pondok Pesantren Tahfidzul Qur’an Sains Ar Risalah Solo serves as an intriguing case for studying the process of cultivating etiquette among its female students. This research employs a field study approach, utilizing observation and interviews with the teachers as the primary methods.

Defining transnational families across countries and time: an analysis of academic discourse on the phenomenon between 2003 and 2023

ABSTRACT In an increasingly globalized, digital world, the way family is conceptualized and practiced is becoming highly diversified and complex. This article investigates the evolving concept of transnational families (TNFs) and aims to elucidate the similarities and differences in how ‘transnational’ and ‘family’ are understood in the academic literature across various contexts. The study is based on an analysis of academic publications on TNF from 2003 to 2023, conducted by a multidisciplinary and multilingual team across several small and medium-sized European countries (both EU and non-EU) with diverse migration histories, family policies and family norms. The analysis reveals varying levels of interest in TNFs and diverse thematic focuses across different contexts over time. It identifies two distinct approaches to conceptualizing TNFs and notes that research continues to primarily focus on dyadic relationships, rarely delving into complex familial networks or addressing non-heteronormative family forms. This highlights the need to further reflect on the ways that globalization and superdiversity are (re)shaping traditional concepts of family and home across different contexts.

Characterization of chemosensory genes in the subterranean pest Gryllotalpa Orientalis based on genome assembly and transcriptome comparison

BackgroundChemosensory perception plays a vital role in insect survival and adaptability, driving essential behaviours such as navigation, mate identification, and food location. This sensory process is governed by diverse gene families, including odorant-binding proteins (OBPs), olfactory receptors (ORs), ionotropic receptors (IRs), chemosensory proteins (CSPs), gustatory receptors (GRs), and sensory neuron membrane proteins (SNMPs). The oriental mole cricket (Gryllotalpa orientalis Burmeister), an invasive pest with an underground, phyllophagous lifestyle, causes substantial crop damage. This study characterizes the chemosensory gene repertoire of G. orientalis based on de novo genome assembly and transcriptomic analysis.ResultsWe present a draft genome of G. orientalis at the scaffold level, spanning 2.94 Gb and comprising 10,497 scaffolds. This assembly encodes 19,155 protein-coding genes, including 158 chemosensory genes: 30 odorant receptors (ORs), 64 ionotropic receptors (IRs), ten gustatory receptors (GRs), 28 odorant-binding proteins (OBPs), 25 chemosensory proteins (CSPs), and a single sensory neuron membrane protein (SNMP). Expression analysis indicated that 71 chemosensory genes were actively expressed in the head, thorax, and legs, with ORs and OBPs showing higher expression in the head and legs. In contrast, GRs and IRs were predominantly expressed in the head.ConclusionsThis study provides the first comprehensive identification of chemosensory gene families in the G. orientalis genome, characterized as a scaffold-level draft genome. These findings provide a basis for future functional studies and highlight the role of chemoreception in the subterranean environment.

The members of zinc finger-homeodomain (ZF-HD) transcription factors are associated with abiotic stresses in soybean: insights from genomics and expression analysis

BackgroundZinc finger homeodomain (ZF-HD) belongs to the plant-specific transcription factor (TF) family and is widely involved in plant growth, development and stress responses. Despite their importance, a comprehensive identification and analysis of ZF-HD genes in the soybean (Glycine max) genome and their possible roles under abiotic stress remain unexplored.ResultsIn this study, 51 ZF-HD genes were identified in the soybean genome that were unevenly distributed on 17 chromosomes. All GmZF-HD genes contained a conserved ZF-HD_dimer domain and had diverse physicochemical features. Furthermore, the GmZF-HD gene structures exhibited 3 to 10 conserved motifs, and most of them showed intronless gene structures. Phylogenetic analysis categorized them into eight major groups with the highest closeness to dicots including Brassica rapa and Malus domestica. The cis-element analysis recognized plant growth and development (10%), phytohormones (31%) and stress-responsive (59%) elements. Synteny analysis identified 73 segmental and 1 tandem duplicated genes that underwent purifying selection. The collinearity analysis revealed that GmZF-HD genes showed higher homology with dicot species, indicating common ancestors with close evolutionary relationships. A total of 94 gma-miRNAs from 41 diverse miRNA families were identified, targeting 40 GmZF-HD genes, with GmZF-HD6 being most targeted by 7 miRNAs, and gma-miR4993 emerging as the dominant miRNA family. Different TFs including ERF, LBD, BBR-BPC and MYB, etc., were predicted in all 51 GmZF-HD genes upstream regions and visualized in the network. Expression profiling through RNA-Seq showed diverse expressions of GmZF-HD genes in different tissues including seeds, roots, shoots and leaves under diverse conditions. Further, the qRT-PCR analysis demonstrated that all tested GmZF-HD genes were significantly induced in soybean leaves, mainly the GmZF-HD5/6/13/39 and GmZF-HD45 genes were significantly upregulated (2.5 to 8.8 folds) under the tested stress treatments compared to control, highlighting their potential roles in response to stresses in soybean.ConclusionOverall, this study reveals comprehensive insights into the ZF-HD genes in soybeans and provides a valuable contribution towards functional studies for soybean improvement under stress conditions.

Inferring Tripartite Associations of Vector-Borne Plant Pathogens Using a Next-Generation Sequencing Approach.

Phytoplasmas are a group of plant-pathogenic, cell-wall-less bacteria vectored primarily by leafhoppers (Hemiptera Cicadellidae), one of the most diverse families of insects. Despite the importance of documenting associations between phytoplasmas, their insect vectors, and plant hosts to prevent disease outbreaks, such knowledge is currently highly incomplete and largely neglects the diversity of the system in natural areas. Here, we used anchored hybrid enrichment (AHE) to recover the DNA of five plant genes (rbcL, matK, ITS1, ITS2, and trnH-psbA) in 58 phloem-feeding leafhoppers from around the world that had previously tested positive for phytoplasma infection. Using BLASTn and a strict filtering approach, we assigned taxonomic classifications to the plant sequences and tested for cophylogenetic signals between potential Deltocephalinae leafhopper vectors and their associated plants. We observed incongruence between plant and insect phylogenies. Many leafhopper species, including presumed grass specialists, fed on distantly related plant lineages; 66% of sampled leafhoppers fed on plants from at least two different orders. By disentangling phytoplasma-leafhopper-plant interactions, we identify locations at risk of phytoplasma disease outbreaks. Furthermore, the observed wide diet breadth raises questions about how phytoplasma infection may manipulate the feeding preference of their insect host and helps fill the gaps in understanding the ecology and diversification of the tripartite association.

Speech-Language Pathologists' Experiences Working With Culturally and Linguistically Diverse Families: A Scoping Review.

Evidence-based practices (EBPs) use evidence (external and internal), clinician expertise, and client/caregiver perspectives to deliver effective, individualized care. Each component of EBP is highly relevant and most effective when implemented together. Families with cultural or linguistic backgrounds different from the mainstream experience inequitable treatment across all disciplines. As the United States' population becomes increasingly diverse, it is paramount for speech-language pathologists (SLPs) to obtain education and support to provide evidence-based, culturally responsive care. This study conducted a scoping review to determine what is known regarding SLPs' perspectives and experiences working with families or children with different cultural or linguistic backgrounds. Articles were included if they (a) contained empirical novel data, were available in English, were peer-reviewed, and were published; (b) included experiences of SLPs who self-reported that they work with children and/or families with cultural and/or linguistic backgrounds different from themselves or the regional mainstream; and (c) reported effects of cultural or linguistic difference on SLP service provision. Results indicated that SLPs encounter many barriers but often exhibit culturally responsive clinical decision making through the consideration of evidence (external and internal) and their clinical expertise and opinion. Multiple strategies for increasing culturally responsive care and areas that would benefit from further research and systemic change were identified. Barriers and solutions to culturally responsive care represented two areas of needed change: personal and within system. Though not all change may occur immediately, the present study offers suggested solutions for SLPs to implement in their clinical practice for increased culturally responsive care. https://doi.org/10.23641/asha.28119836.

Identification of novel PfEMP1 variants containing domain cassettes 11, 15 and 8 that mediate the Plasmodium falciparum virulence-associated rosetting phenotype.

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a diverse family of variant surface antigens, encoded by var genes, that mediates binding of infected erythrocytes to human cells and plays a key role in parasite immune evasion and malaria pathology. The increased availability of parasite genome sequence data has revolutionised the study of PfEMP1 diversity across multiple P. falciparum isolates. However, making functional sense of genomic data relies on the ability to infer binding phenotype from var gene sequence. For P. falciparum rosetting, the binding of infected erythrocytes to uninfected erythrocytes, the analysis of var gene/PfEMP1 sequences encoding the phenotype is limited, with only eight rosette-mediating PfEMP1 variants described to date. These known rosetting PfEMP1 variants fall into two types, characterised by N-terminal domains known as "domain cassette" 11 (DC11) and DC16. Here we test the hypothesis that DC11 and DC16 are the only PfEMP1 types in the P. falciparum genome that mediate rosetting, by examining a set of thirteen recent culture-adapted Kenyan parasite lines. We first analysed the var gene/PfEMP1 repertoires of the Kenyan lines and identified an average of three DC11 or DC16 PfEMP1 variants per genotype. In vitro rosette selection of the parasite lines yielded four with a high rosette frequency, and analysis of their var gene transcription, infected erythrocyte PfEMP1 surface expression, rosette disruption and erythrocyte binding function identified four novel rosette-mediating PfEMP1 variants. Two of these were of the predicted DC11 type (one showing the dual rosetting/IgM-Fc-binding phenotype), whereas two contained DC15 (DBLα1.2-CIDRα1.5b) a PfEMP1 type not previously associated with rosetting. We also showed that a Thai parasite line expressing a DC8-like PfEMP1 binds to erythrocytes to form rosettes. Hence, these data expand current knowledge of rosetting mechanisms and emphasize that the PfEMP1 types mediating rosetting are more diverse than previously recognised.

Pangeneric genome analyses reveal the evolution and diversity of the orchid genus Dendrobium.

Orchids constitute one of the most diverse families of angiosperms, yet their genome evolution and diversity remain unclear. Here we construct and analyse chromosome-scale de novo assembled genomes of 17 representative accessions spanning 12 sections in Dendrobium, one of the largest orchid genera. These accessions represent a broad spectrum of phenotypes, lineages and geographical distributions. We first construct haplotype-resolved genomes for a Dendrobium hybrid and uncover haplotypic variations and allelic imbalance in the heterozygous genome, demonstrating the significance of diverse ancestry. At Dendrobium genus-wide scale, we further elucidate phylogenetic relationships, evolutionary dynamics, entire gene repertoire, and the mechanisms of preserving ancient genetic variants and rapid recent genome evolution for habitat adaption. We also showcase distinctive evolutionary trajectories in MADS-box and PEBP families over 28 Ma. These results considerably contribute to unearthing the mystery of orchid origin, evolution and diversification, laying the foundation for efficient use of genetic diversity in breeding.

Distinct properties and activation of hexameric and heptameric Pannexin 1 channel concatemers.

Pannexin 1 (PANX1) is a member of a topologically related and stoichiometrically diverse family of large pore membrane ion channels that support the flux of signaling metabolites (e.g., ATP) and fluorescent dyes. High-resolution structural analyses have identified PANX1 as a heptamer despite early evidence suggesting that it might be a hexamer. To determine if PANX1 channel activity is supported in both hexameric and heptameric conformations, we examined properties of concatenated PANX1 constructs comprising either six or seven subunits with intact or truncated C-termini (the latter to mimic caspase-cleavage activation). In whole-cell recordings from PANX1-deleted cells, the C-tail-truncated hexameric and heptameric concatemers generated outwardly rectifying PANX1-like currents only after severing the intersubunit linkers. Surprisingly, α1D adrenoceptor stimulation activated constructs with intact or truncated C-tails, even without linker cleavage. In inside-out patches from PANX1-deleted cells, linker cleavage activated C-tail truncated channels derived from either hexameric or heptameric concatemers. The heptamers presented peak unitary conductance and mean open time that was similar to channels assembled from the expression of unlinked single PANX1 subunits and greater than from the hexamers. In addition, the linker-cleaved heptameric concatemers supported greater PANX1-dependent ATP release and TO-PRO-3 uptake than the corresponding hexamers. These data indicate that functional PANX1 channels can be obtained in either hexameric or heptameric conformations and suggest that the distinct unitary properties of heptameric channels are more conducive to large molecule permeation by PANX1; they also suggest that there are distinct structural requirements for C-tail cleavage and receptor-mediated PANX1 activation mechanisms.

Identifying genetic susceptibility loci associated with human coronary artery disease.

Coronary artery disease (CAD) is a multigenic condition influenced by both nature and nurture (60% to 40%). Prognosis of CAD is based on familial patterns. This study examined and analyzed the susceptibility of CAD to genetic variants in various Pakistani families. A total of 50 families, 308 participants (79 affected and 229 unaffected were genotyped for NOS3 (rs1799983, rs2070744), PON1 (rs662), LPA-PLA2 (rs105193, rs1805017), APOE (rs429358, rs7412), PCSK9 (rs505151), MEF2A (rs325400), TNF (rs1800629) and LDLR (rs1122608, rs2228671) genes. The family-based association in CAD associated genes SNPs were NOS3 (rs1799983), PON1 (rs662), LPA-PLA2 (rs1805017), MEF2A (rs325400), and LDLR (rs1122608, rs222867) showed transmission within families p≤ 0.05 whereas NOS3 (rs2070744), APOE (rs429358, rs7412) and TNF (rs1800629) showed no association TDT asymptotic p-value >0.05. In DFAM and QFAM test NOS3 (rs1799983), PON1 (rs662), MEF2A (rs325400), and LDLR (rs1122608, rs222867) showed positive association p≤ 0.05 in both whereas NOS3 (rs2070744), APOE (rs429358, rs7412), LPA-PLA2 (rs1805017) and TNF (rs1800629) showed low risk of transmission asymptotic p-value >0.05 in DFAM but NOS3(rs2070744), APOE(rs7412), LPA-PLAG2(rs1805017) also showed association p≤ 0.05 whereas APOE (rs429358) and TNF (rs1800629) showed no association EMP1 p-value >0.05 in QFAM. In linkage analysis Chromosome 6 (Position 70.810): LOD = 3.16, Chromosome 7 (Position 107.190): LOD = 3.16, and chromosome 19 (Position 31.470): LOD = 3.90 also showed significant association with disease as p < 0.05. This discovery enhances the understanding about genetic variants of CAD and also facilitates early detection, targeted interventions, pattern of inheritance in population. This ultimately improving patient outcomes and guiding future research to highlight its significance as a potential diagnostic marker.

Altered sphingolipid profile in response to skeletal muscle injury in a mouse model of type 1 diabetes mellitus.

A complication of type 1 diabetes mellitus (T1DM) is diabetic myopathy that includes reduced regenerative capacity of skeletal muscle. Sphingolipids are a diverse family of lipids with roles in skeletal muscle regeneration. Some studies have found changes in sphingolipid species levels in T1DM, however, the effect of T1DM on a sphingolipid panel in regenerating skeletal muscle has not been examined. Wild-type (WT) and diabetic Ins2Akita+/- (Akita) mice received cardiotoxin-induced muscle injury in their left quadriceps, gastrocnemius-plantaris-soleus, and tibialis anterior muscles with the contralateral muscles serving as uninjured controls. Muscles were collected at 1, 3, 5, or 7 days postinjury. In regenerating muscle from Akita mice, lipid staining with BODIPY 493/503 revealed increased intramyocellular and total lipids and perilipin-1-positive cell numbers as compared with WT. Liquid chromatography-mass spectrometry of quadriceps was used to identify sphingolipid levels in skeletal muscle. The C22:0 and C24:0 ceramides were significantly elevated in uninjured Akita, whereas ceramide C24:1 was decreased in injured Akita compared with WT. Ceramide-1-phosphate was increased in Akita compared with WT regardless of injury, whereas sphingosine-1-phosphate (S1P) was elevated with injury in WT but this response was muted in Akita mice. Western blotting of key enzymes involved in sphingolipid metabolism revealed S1P lyase, the enzyme that degrades S1P irreversibly, was significantly elevated in the injured muscle in Akita mice during regeneration, in accordance with lower S1P levels. This mouse model of T1DM demonstrates sphingolipidomic changes that may contribute to delayed muscle regeneration.NEW & NOTEWORTHY Muscle lipids become elevated, and the sphingolipid profile is altered by T1DM in skeletal muscle regeneration. A loss of S1P is accompanied by greater expression of sphingosine-1-phosphate lyase (SPL) in response to injury in Akita mice, suggesting a role for sphingolipids in the attenuated repair of skeletal muscle in T1DM rodent models. Although ceramide-1-phosphate (C1P) is increased with T1DM, there was no increase in ceramide kinase (CerK) suggesting an alternative route of ceramide phosphorylation in skeletal muscle.

Caregiver-child Neural Synchrony: Magic, Mirage, or Developmental Mechanism?

Young children transition in and out of more and less synchronous states with their caregivers across physiology, behavior, and brain activity, but what do these synchronous periods mean? One body of caregiver-child two-brain studies using fNIRS finds that individual, familial, and moment-to-moment behavioral and contextual factors are correlated with caregiver-child neural synchrony, while a separate body of literature finds that neural synchrony is associated with positive child outcomes. Taken together, it is tempting to conclude that caregiver-child neural synchrony may act as a key developmental mechanism linking children’s experiences to their healthy development, but many questions remain. In this brief review, we synthesize key findings and open questions from two-brain caregiver-child studies using functional near-infrared spectroscopy (fNIRS), which is uniquely well-suited for use with caregivers and children, but also laden with unique constraints. Throughout, we highlight key questions and best practice recommendations to optimize two-brain fNIRS to examine these critical developmental mechanisms from assumptions guiding study design to final analysis. We particularly emphasize the need to consider immediate and global stressors as context for interpretation of neural synchrony findings, and the need for full inclusion of socioeconomically and racially diverse families in future studies.

Comparative toxicological analysis of different plant leaf extracts from diverse families targeting rice moth, Corcyra cephalonica (Lepidoptera: Pyralidae)

Comparative toxicological analysis of different plant leaf extracts from diverse families targeting rice moth, Corcyra cephalonica (Lepidoptera: Pyralidae)

LENGTH-WEIGHT RELATIONSHIP OF 55 SPECIES OF FISH CAUGHT BY ARROWHEAD FIXED TRAPS “CURRAIS” IN THE COAST OF PERNAMBUCO (BRAZIL)

The length-weight relationship (LWR) of 55 species was estimated in captures conducted in arrowhead fixed traps off the northern coast of Pernambuco (Northeast, Brazil) using frequentist and Bayesian statistical approaches. The fish were obtained between the months of May 2014 and November 2020, with one or two days of sampling each month. The most diverse families were Carangidae and Haemulidae with 13 and 10 species, respectively. A new maximum length was recorded for Oligoplites saurus. Except for Odontoscion dentex, all other species exhibited condition factor values greater than 1.00. The species Chloroscombrus chrysurus, Diapterus auratus, Diapterus rhombeus, Harengula clupeola, O. saurus, and Opisthonema oglinum showed continuations of their estimates from previous Bayesian analyses, contributing to the improvement of data for these species. Keywords: Somatic growth, traditional fishing, tropical waters, condition factor, bayesiananalysis.