Phytophagous mites are dangerous pests, causing economic losses to the world's crops. Nanotechnology is a promising field for pests and disease management, and enhancement of agricultural productivity. The aim of the study was to evaluation of the effectiveness of copper nanoparticles (CuNP) against all stages of eriophyid mite, the citrus rust mite, Phyllocoptruta oleivora (Ashmead) (Acari: Eriophyidae) and tetranychid mite, the citrus brown mite Eutetranychus orientalis (Klein) (Acari: Tetranychidae), and Tenuipalpid mite, the false spider mite, Brevipalpus obovatus Donnadieu (Acari: Tenuipalpidae). This includes its impacts on predacious mites, Amblyseius swirskii Athias-Henriot and Euseius scutalis (Athias-Henriot) (Acari: Phytoseiidae), on orange trees under field conditions. Five different concentrations of copper nanoparticles (40, 80, 160, 240, and 320 ppm), as well as the control (well water) were examined. The obtained results indicated that the mortality rate of both phytophagous and predacious mites was associated with an increase in the concentrations of copper nanoparticles. Copper nanoparticles were significantly effective in killing P. oleivora, E. orientalis and B. obovatus with minimal effects on A. swirskii and E. scutalis. The mortality percentage was 15.24, 20.32, 46.32, 78.97 and 86.37% for P. oleivora, 6.87, 9.86, 28.91, 56.30 and 77.52% for E. orientalis and 8.38, 23.50, 48.83, 68.80 and 84.08% for B. obovatus while the mortality percentage was 0.00, 0.56, 5.83, 9.91 and 15.19% for A. swirskii and 0.44, 3.96, 6.93, 8.63 and 21.39% for E. scutalis one week after exposure to 40, 80, 160, 240 and 320 ppm of copper nanoparticles, respectively. Moreover, the results showed that copper nanoparticles caused a reduction in the percentage of eggs hatching. The percentages of larvae hatching from eggs were 96.29, 80.00, 64.13, 45.66 and 32.17% for P. oleivora, 97.38, 83.28, 69.41, 48.01 and 35.29 for E. orientalis and 96.60, 76.92, 56.38, 40.55 and 33.28% for B. obovatus one week after exposure to copper nanoparticles at 40, 80, 160, 240 and 320 ppm respectively, compared with the control (well water). According to the results, the use of copper nanoparticles significant effect on reducing the population of phytophagous mites associated with orange trees, with low detrimental effects on predatory mites.