In the upper agricultural belt of the tropical Andes, above 3000 m of altitude, long fallow agriculture is commonly used to produce potatoes and cereals. This agricultural system alternates short intervals of production (2–4 years) with long fallow periods (generally 5–10 years or more). In order to analyse soil changes related to the rapid fertility loss during the production interval and soil fertility restoration during the fallow period, the soils of two adjacent but contrasting plots were compared. One plot had passed through 15 years of fallow (restored) and the other had been cultivated with potatoes for three consecutive years after a 12 years fallow period (depleted). The soils were analysed for fresh C- and N-microbial biomass, inorganic N and physical–chemical properties. Then, the soils were incubated under controlled laboratory conditions for 81 days, with and without the addition of 14 C labelled wheat straw. During the incubation, periodic measurement of C– and 14 C –CO 2 release, C- and 14 C -microbial biomass, N-microbial biomass and inorganic N were performed. The most pronounced difference between the fresh soils was in the microbial biomass, which was almost double the amount in the restored soil with respect to the depleted soil. During the incubation, the total amounts of soil native C and N mineralised were significantly higher in the restored soil. Also the mineralisation of the added 14 C -labelled straw was faster in the restored than in depleted soil. These results suggest that the fallow period leads to an increase in the labile C and N pools and microbial biomass, which represent more carbon and nitrogen availability for microorganisms and plants and could be related to fertility restoration. A very high stability of the organic matter in these mountain soils was also revealed and its implications for agricultural sustainability are discussed.
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