Failure Rate Ratio Research Articles

Presynaptic Enhancement of Transmission from Nociceptors Expressing Nav1.8 onto Lamina-I Spinothalamic Tract Neurons by Spared Nerve Injury in Mice.

Alteration of synaptic function in the dorsal horn (DH) has been implicated as a cellular substrate for the development of neuropathic pain, but certain details remain unclear. In particular, the lack of information on the types of synapses that undergo functional changes hinders the understanding of disease pathogenesis from a synaptic plasticity perspective. Here, we addressed this issue by using optogenetic and retrograde tracing ex vivo to selectively stimulate first-order nociceptors expressing Nav1.8 (NRsNav1.8) and record the responses of spinothalamic tract neurons in spinal lamina I (L1-STTNs). We found that spared nerve injury (SNI) increased excitatory postsynaptic currents (EPSCs) in L1-STTNs evoked by photostimulation of NRsNav1.8 (referred to as Nav1.8-STTN EPSCs). This effect was accompanied by a significant change in the failure rate and paired-pulse ratio of synaptic transmission from NRsNav1.8 to L1-STTN and in the frequency (not amplitude) of spontaneous EPSCs recorded in L1-STTNs. However, no change was observed in the ratio of AMPA to NMDA receptor-mediated components of Nav1.8-STTN EPSCs or in the amplitude of unitary EPSCs constituting Nav1.8-STTN EPSCs recorded with extracellular Ca2+ replaced by Sr2+ In addition, there was a small increase (approximately 10%) in the number of L1-STTNs showing immunoreactivity for phosphorylated extracellular signal-regulated kinases in mice after SNI compared with sham. Similarly, only a small percentage of L1-STTNs showed a lower action potential threshold after SNI. In conclusion, our results show that SNI induces presynaptic modulation at NRNav1.8 (consisting of both peptidergic and nonpeptidergic nociceptors) synapses on L1-STTNs forming the lateral spinothalamic tract.

Synaptic effects of xenon on NMDA receptor-mediated response in rat spinal neuron

To investigate the precise mechanism of xenon (Xe), pharmacologically isolated AMPA/KA and NMDA receptor-mediated spontaneous (s) and evoked (e) excitatory postsynaptic currents (s/eEPSCAMPA/KA and s/eEPSCNMDA) were recorded from mechanically isolated single spinal sacral dorsal commissural nucleus (SDCN) neurons attached with glutamatergic nerve endings (boutons) using conventional whole-cell patch-clamp technique. We analysed kinetic properties of both s/eEPSCAMPA/KA and s/eEPSCNMDA by focal single- and/or paired-pulse electrical stimulation to compare them. The s/eEPSCNMDA showed smaller amplitude, slower rise time, and slower 1/e decay time constant (τDecay) than those of s/eEPSCAMPA/KA. We previously examined how Xe modulates s/eEPSCAMPA/KA, therefore, examined the effects on s/eEPSCNMDA in the present study. Xe decreased the frequency and amplitude of sEPSCNMDA, and decreased the amplitude but increased the failure rate and paired-pulse ratio of eEPSCNMDA without affecting their τDecay. It was concluded that Xe might suppress NMDA receptor-mediated synaptic transmission via both presynaptic and postsynaptic mechanisms.

Outcomes of Kidney Transplants From Toxoplasma-Positive Donors: An Organ Procurement and Transplant Network Database Analysis.

There is a need to reconsider the acceptance of organs from donors considered suboptimal, in the absence of data. Toxoplasma antibody-positive donors (TPD) constitute one such group. The objective of our study was to compare graft survival in deceased donor renal transplant (Tx) recipients, stratified by Toxoplasma IgG status, using the Organ Procurement and Transplantation Network (OPTN) database. A log-linear event history regression model for graft failure categorized by Toxoplasma IgG status, adjusting for confounders was applied to first kidney-only Tx recipients from 2018 to 2022. Of the 51,422 Tx, 4,317 (8.4%) were from TPD. Acute rejection and graft failure (5% each) were similar between groups. Crude graft failure was 7.3 failures per 100 person-years for TPD recipients compared to 6.5 failures per 100 person-years for the Toxoplasma-negative group (p 0.008). The crude failure rate ratio was 1.14 with an adjusted hazard rate ratio of 1.04 (95% CI: 0.94, 1.15, p 0.39). In renal Tx recipients, TPD graft recipients have comparable survival to Tx from Toxoplasma-negative recipients. While caution and close monitoring of recipients post-Tx for surveillance of disseminated toxoplasmosis are still warranted, our study suggests that patients can be successfully managed using TPD organs.

Life Assessment for Motorized Spindle with Zero Traumatic Failure Data Based on Subdistribution Competing Risk Model

Considering the influence of performance degradation on a product’s traumatic failure, under the condition that only degradation data are observed and no traumatic failure data are observed, this paper proposes a subdistribution competing risk model to achieve a motorized spindle life assessment. This paper assumes that the failure rate ratio of the tested products does not change with time under different stress levels. Basic reliability with zero traumatic failure data is modeled by a unilateral confidence limit method under a two-parameter Weibull distribution. Performance degradation data are taken as covariates. The regression coefficients of the covariates are calculated by SPSS software. Then, a subdistribution competing risk model is constructed, which reflects the dependency relationship between reliability and performance degradation, and the product’s reliability life can be evaluated accordingly. The correctness and advantages of the model built in this paper are verified by a case analysis combined with the performance degradation information of a motorized spindle.

Optimized Resource Allocation in IoT Using Fuzzy Logic and Bio-Inspired Algorithms

IoT smart devices are a confluence of microprocessors, sensors, power source and transceiver modules to effectively sense, communicate and transfer data. Energy efficiency is a key governing value of the network performance of smart devices in distributed IoT networks. Low and discrete power and limited amount of memory and finite number of resources form some major bottlenecks in the workflow. Dynamic load balancing, reliability and flexibility are heavily relied upon by cloud computing for its accessibility. Resources are dynamically provided to the end client in an as-come on-demand fashion with the global network that is the Internet. Proportionally the need for services is increasing at a rate that is astonishing compared to any other forms of development. Load balancing seems a major challenge faced due to the architecture and the modular nature of our cloud environment. Loads need to be distributed dynamically to all the nodes. In this paper, we have introduced a technique that combines fuzzy logic with various nature inspired algorithms—grey wolf algorithm and firefly algorithm to effectively balance the load in a network of IoT devices. The performances of various nature inspired algorithms are compared with a brute force approach based on energy efficiency, network lifetime maximization, node failure rate and packet delivery ratio.

Magnetic nanoparticle technique versus radioisotope technique in detection of sentinel lymph node in early breast cancer: a systematic review and meta-analysis

Sentinel lymph node biopsy is the standard technique to stage the axilla in early breast cancer. The gold standard is the dual technique of radioisotope and blue dye injection. The drawbacks of dual technique include handling and disposal of radioactive material. Equally reliable, user and patient friendly, magnetic tracer super paramagnetic iron oxide, was compared with the radioisotope tracer in detection of Sentinel lymph node in a clinically node negative axilla in early breast cancer in this meta-analysis, with emphasis on the utility and safety of both techniques. PubMed, Medline were searched from April 2015 to October 2019. 1395 patients’ data was included from seven homogenous studies in this meta-analysis. A statistical analysis was performed using STATA 16.1 version for sentinel lymph node detection rate using standard technique, magnetic tracer technique and both techniques. Ratio of successes and failures of the outcomes was measured and analysed. A paired two sample ‘Z’ test was performed to compare between the standard technique and magnetic tracer techniques. The standardised mean difference of ratio of success rate between two techniques was; 1.013334 with a p value of 0.3136.The standardised mean difference of ratio of failure rate between two techniques was 1.016667, with a p value of 0.3132. Success and failure rate showed statistically no significant difference between the two techniques. This two-way analysis with paired two sample ‘Z’ test confirms that neither standard technique nor magnetic tracer techniques are superior or inferior to each other.

Unequal Failure Protection Coding Technique for Distributed Cloud Storage Systems

In recent years, erasure codes have become the de facto standard for data protection in large scale distributed cloud storage systems at the cost of an affordable storage overhead. However, traditional erasure coding schemes, such as Reed-Solomon codes, suffer from high reconstruction cost and I/Os. The recent past has seen a plethora of efforts to optimize the tradeoff between the reconstruction cost, I/Os and storage overhead. Quiet different from all prior studies, in this paper, our erasure coding technique makes the first attempt to take advantage of the unequal failure rates across the disks/nodes to optimize the system reliability and reconstruction performance. Specifically, our proposed technique, the Unequal Failure Protection based Local Reconstruction Code (UFP-LRC) divides the data blocks into several unequal-sized groups with local parities, assigning the data blocks stored on more failure-prone disks/nodes into the smaller-sized group, so as to provide unequal failure protection for each group. In this way, by exploiting the nonuniform local parity degrees, the proposed UFP-LRC enables the data blocks that are stored on more failure-prone disks/nodes to tolerate a greater number of failures while suffering from less repair cost than others, leading to a substantial improvement of the overall reliability and repair performance for cloud storage systems. We perform numerical analysis and build a prototype storage system to verify our approach. The analytical results show that the UFP-LRC technique gradually outperforms LRC along the increase of failure rate ratio. Also, extensive experiments show that, when compared to LRC, UFP-LRC is able to achieve a 10 to 15 percent improvement in throughput, and an 8 to 12 percent reduction in decoding latency, while retaining a comparable overall reliability.

Effects of nitrous oxide on glycinergic transmission in rat spinal neurons

We investigated the effects of nitrous oxide (N2O) on glycinergic inhibitory whole-cell and synaptic responses using a “synapse bouton preparation,” dissociated mechanically from rat spinal sacral dorsal commissural nucleus (SDCN) neurons. This technique can evaluate pure single- or multi-synaptic responses from native functional nerve endings and enable us to accurately quantify how N2O influences pre- and postsynaptic transmission. We found that 70 % N2O enhanced exogenous glycine-induced whole-cell currents (IGly) at glycine concentrations lower than 3 × 10–5 M, but did not affect IGly at glycine concentrations higher than 10–4 M. N2O did not affect the amplitude and 1/e decay-time of both spontaneous and miniature glycinergic inhibitory postsynaptic currents recorded in the absence and presence of tetrodotoxin (sIPSCs and mIPSCs, respectively). The decrease in frequency induced by N2O was observed in sIPSCs but not in mIPSCs, which was recorded in the presence of both tetrodotoxin and Cd2+, which block voltage-gated Na+ and Ca2+ channels, respectively. N2O also decreased the amplitude and increased the failure rate and paired-pulse ratio of action potential-evoked glycinergic inhibitory postsynaptic currents. N2O slightly decreased the Ba2+ currents mediated by voltage-gated Ca2+ channels in SDCN neurons. We found that N2O suppresses glycinergic responses at synaptic levels with presynaptic effect having much more predominant role. The difference between glycinergic whole-cell and synaptic responses suggests that extrasynaptic responses seriously modulate whole-cell currents. Our results strongly suggest that these responses may thus in part explain analgesic effects of N2O via marked glutamatergic inhibition by glycinergic responses in the spinal cord.

Xenon modulates the GABA and glutamate responses at genuine synaptic levels in rat spinal neurons

Effects of xenon (Xe) on whole-cell currents induced by glutamate (Glu), its three ionotropic subtypes, and GABA, as well as on the fast synaptic glutamatergic and GABAergic transmissions, were studied in the mechanically dissociated “synapse bouton preparation” of rat spinal sacral dorsal commissural nucleus (SDCN) neurons. This technique evaluates pure single or multi-synapse responses from native functional nerve endings and enables us to quantify how Xe influences pre- and postsynaptic transmissions accurately. Effects of Xe on glutamate (Glu)-, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-, kainate (KA)- and N-methyl-d-aspartate (NMDA)- and GABAA receptor-mediated whole-cell currents were investigated by the conventional whole-cell patch configuration. Excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) were measured as spontaneous (s) and evoked (e) EPSCs and IPSCs. Evoked synaptic currents were elicited by paired-pulse focal electric stimulation. Xe decreased Glu, AMPA, KA, and NMDA receptor-mediated whole-cell currents but did not change GABAA receptor-mediated whole-cell currents. Xe decreased the frequency and amplitude but did not affect the 1/e decay time of the glutamatergic sEPSCs. Xe decreased the frequency without affecting the amplitude and 1/e decay time of GABAergic sIPSCs. Xe decreased the amplitude and increased the failure rate (Rf) and paired-pulse ratio (PPR) without altering the 1/e decay time of both eEPSC and eIPSC, suggesting that Xe acts on the presynaptic side of the synapse. The presynaptic inhibition was greater in eEPSCs than in eIPSCs. We conclude that Xe decreases glutamatergic and GABAergic spontaneous and evoked transmissions at the presynaptic level. The glutamatergic presynaptic responses are the main target of anesthesia-induced neuronal responses. In contrast, GABAergic responses minimally contribute to Xe anesthesia.

Correction of fractional cycle bias of pseudolite system for user integer ambiguity resolution

Synchronized pseudolite systems allow single-point positioning (SPP) using carrier-phase measurements with centimeter-level accuracy. Locata, developed by the Locata Corporation, emerged from the development of synchronized pseudolite technology. Numerous studies have claimed to achieve Locata SPP with centimeter-level accuracy; however, the ambiguities of Locata observations have been typically estimated as floating-point values. Similar to precise point positioning of global navigation satellite systems, pseudolite SPP that is based on ambiguity fixing can improve the positioning accuracy and shorten the convergence time, compared to the float solution. However, integer ambiguity fixing may be prevented due to the clock offset among the pseudolite transmitters. To recover the integer nature of single-difference ambiguity, a fractional cycle bias (FCB) correction method is proposed; this method is based on two-way time synchronization. The FCB correction method employed to estimate and correct the bias by a pseudolite network itself has two steps: two-way FCB measurement and half-cycle bias estimation. A ground-based testbed is constructed to test and verify the proposed method. It is shown that the FCB between the pseudolite transmitters have a stability better than 15 ps even considering the influence of variable multipath delay; the bias can be estimated with high precision and reliability. Statistical results support the theoretical findings that half-cycle bias may be caused by two-way clock offset measuring via carrier phase measurement. A two-dimensional positioning experiment is performed to evaluate the performance of the proposed FCB correction method. The single-difference ambiguities are fixed to integer values using the LAMBDA method with the fixed failure-rate ratio test. The results indicated that the positioning accuracy is at the sub-centimeters to centimeter-level compared with the real-valued solutions for all surveyed points.

Effects of triphenyltin on glycinergic transmission on rat spinal neurons

Glycine is a fast inhibitory transmitter like γ-aminobutyric acid in the mammalian spinal cord and brainstem, and it is involved in motor reflex, nociception, and neuronal development. Triphenyltin (TPT) is an organometallic compound causing environmental hazard to many wild creatures. Our previous findings show that TPT ultimately induces a drain and/or exhaustion of glutamate in excitatory presynaptic nerve terminals, resulted in blockage of neurotransmission as well as methylmercury. Therefore, we have investigated the neurotoxic mechanism how TPT modulates inhibitory glycinergic transmission in the synaptic bouton preparation of rat isolated spinal neurons using a patch clamp technique. TPT at environmentally relevant concentrations (3–300 nM) significantly increased the number of frequency of glycinergic spontaneous and miniature inhibitory postsynaptic currents (sIPSC and mIPSC) without affecting the current amplitude and decay time. The TPT effects were also observed in external Ca2+-free solution containing tetrodotoxin (TTX) but removed in Ca2+-free solution with both TTX and BAPTA-AM (Ca2+ chelator). On the other hand, the amplitude of glycinergic evoked inhibitory postsynaptic currents (eIPSCs) increased with decreasing failure rate (Rf) and paired pulse ratio (PPR) in the presence of 300 nM TPT. At a high concentration (1 µM), TPT completely blocked eIPSCs after a transient facilitation. Overall, these results suggest that TPT directly acts transmitter-releasing machinery in glycinergic nerve terminals. Effects of TPT on the nerve terminals releasing fast transmitters were greater in the order of glycinergic > glutamatergic > GABAergic ones. Thus, TPT is supposed to cause a strong synaptic modulations on glycinergic neurotransmission in wild creatures.

Histamine H3 Heteroreceptors Suppress Glutamatergic and GABAergic Synaptic Transmission in the Rat Insular Cortex.

Histamine H3 receptors are autoreceptors that regulate histamine release from histaminergic neuronal terminals. The cerebral cortex, including the insular cortex (IC), expresses abundant H3 receptors; however, the functions and mechanisms of H3 receptors remain unknown. The aim of this study was to elucidate the functional roles of H3 in synaptic transmission in layer V of the rat IC. Unitary excitatory and inhibitory postsynaptic currents (uEPSCs and uIPSCs) were obtained through paired whole-cell patch-clamp recording in cerebrocortical slice preparations. The H3 receptor agonist, R-α-methylhistamine (RAMH), reduced the uEPSC amplitude obtained from pyramidal cell to pyramidal cell or GABAergic interneuron connections. Similarly, RAMH reduced the uIPSC amplitude in GABAergic interneuron to pyramidal cell connections. RAMH-induced decreases in both the uEPSC and uIPSC amplitudes were accompanied by increases in the failure rate and paired-pulse ratio. JNJ 5207852 dihydrochloride or thioperamide, H3 receptor antagonists, inhibited RAMH-induced suppression of uEPSCs and uIPSCs. Unexpectedly, thioperamide alone increased the uIPSC amplitude, suggesting that thioperamide was likely to act as an inverse agonist. Miniature EPSC or IPSC recordings support the hypothesis that the activation of H3 receptors suppresses the release of glutamate and GABA from presynaptic terminals. The colocalization of H3 receptors and glutamate decarboxylase or vesicular glutamate transport protein 1 in presynaptic axon terminals was confirmed through double pre-embedding microscopy, using a combination of pre-embedding immunogold and immunoperoxidase techniques. The suppressive regulation of H3 heteroreceptors on synaptic transmission might mediate the regulation of sensory information processes, such as gustation and visceral sensation, in the IC.

SOME SUFFICIENT CONDITIONS FOR RELATIVE AGING OF LIFE DISTRIBUTIONS

In reliability theory, Cox's proportional hazard model is quite popular and widely used. In many situations, it is observed that failure rates under consideration are not proportional, rather they cross each other. In such situations, an alternative to Cox's proportional hazard model may be monotone hazard ratio model (provided the ratio exists). A notion of relative aging based on increasing hazard ratio was introduced by Kalashnikov and Rachev [19]. Sengupta and Deshpande [40] further explored this model and posited two other notions of relative aging based on increasing reversed failure rate ratio and increasing mean residual life ratio. In this study, for two life distributions, we derive sufficient conditions under which a life distribution ages faster than the other with respect to notions of relative aging described above. These sufficient conditions are easy to verify and can be used in practical applications where one is interested in studying relative aging of two life distributions. Applications of these results to relative aging of weighted distributions have also been illustrated. We also introduce a new relative aging ordering in terms of mean inactivity time order and study its fundamental properties.

A data driven partial ambiguity resolution: Two step success rate criterion, and its simulation demonstration

Ambiguity Resolution (AR) is a key technique in GNSS precise positioning. In case of weak models (i.e., low precision of data), however, the success rate of AR may be low, which may consequently introduce large errors to the baseline solution in cases of wrong fixing. Partial Ambiguity Resolution (PAR) is therefore proposed such that the baseline precision can be improved by fixing only a subset of ambiguities with high success rate.This contribution proposes a new PAR strategy, allowing to select the subset such that the expected precision gain is maximized among a set of pre-selected subsets, while at the same time the failure rate is controlled. These pre-selected subsets are supposed to obtain the highest success rate among those with the same subset size. The strategy is called Two-step Success Rate Criterion (TSRC) as it will first try to fix a relatively large subset with the fixed failure rate ratio test (FFRT) to decide on acceptance or rejection. In case of rejection, a smaller subset will be fixed and validated by the ratio test so as to fulfill the overall failure rate criterion. It is shown how the method can be practically used, without introducing a large additional computation effort. And more importantly, how it can improve (or at least not deteriorate) the availability in terms of baseline precision comparing to classical Success Rate Criterion (SRC) PAR strategy, based on a simulation validation. In the simulation validation, significant improvements are obtained for single-GNSS on short baselines with dual-frequency observations. For dual-constellation GNSS, the improvement for single-frequency observations on short baselines is very significant, on average 68%. For the medium- to long baselines, with dual-constellation GNSS the average improvement is around 20–30%.

An Efficient Implementation of Fixed Failure-Rate Ratio Test for GNSS Ambiguity Resolution

Ambiguity Resolution (AR) plays a vital role in precise GNSS positioning. Correctly-fixed integer ambiguities can significantly improve the positioning solution, while incorrectly-fixed integer ambiguities can bring large positioning errors and, therefore, should be avoided. The ratio test is an extensively used test to validate the fixed integer ambiguities. To choose proper critical values of the ratio test, the Fixed Failure-rate Ratio Test (FFRT) has been proposed, which generates critical values according to user-defined tolerable failure rates. This contribution provides easy-to-implement fitting functions to calculate the critical values. With a massive Monte Carlo simulation, the functions for many different tolerable failure rates are provided, which enriches the choices of critical values for users. Moreover, the fitting functions for the fix rate are also provided, which for the first time allows users to evaluate the conditional success rate, i.e., the success rate once the integer candidates are accepted by FFRT. The superiority of FFRT over the traditional ratio test regarding controlling the failure rate and preventing unnecessary false alarms is shown by a simulation and a real data experiment. In the real data experiment with a baseline of 182.7 km, FFRT achieved much higher fix rates (up to 30% higher) and the same level of positioning accuracy from fixed solutions as compared to the traditional critical value.

Glass half full: Survival analysis of new rural doctor retention in Western Australia.

To measure the time trends in retention of new rural doctors in Western Australia (WA) and identify factors associated with improved retention. Retrospective inception cohort study of the 1154 doctors first commencing rural practice in WA in 2004-2013, who provided 1222 tours of service consisting of up to eight attachments at different rural practice settings. Failure of doctor retention as evidenced by an absence from the rural medical workforce of greater than 1 year and analysed using actuarial survival methods and Cox proportional hazards regression. Comparing 2009-2013 with 2004-2008, there was an improvement of 10 percentage points in retention of new rural doctors at 2 years (58% versus 48% ) and 7 percentage points at 5 years (38% versus 31%). The retention failure rate ratio was 0.68 (95% CI, 0.58-0.83). The improvement at 5 years was largely attributable to gains in retention of those who began as GP registrars (37% versus 14%). Failure of doctor retention was lower in those who possessed procedural skills (RR 0.61, 95% CI, 0.47-0.78) and lower in international medical graduates than in those trained in Australia (RR 0.75, 95% CI, 0.59-0.95). New rural GP retention in WA has improved substantially, an observation at least consistent with government initiatives delivering a positive return. However, it remains the case that the majority of new doctors have left rural practice within 5 years of commencing their tour of service.

Modulation of inhibitory and excitatory fast neurotransmission in the rat CNS by heavy water (D2O).

The effects of heavy water (deuterium oxide, D2O) on GABAergic and glutamatergic spontaneous and evoked synaptic transmission were investigated in acute brain slice and isolated "synaptic bouton" preparations of rat hippocampal CA3 neurons. The substitution of D2O for H2O reduced the frequency and amplitude of GABAergic spontaneous inhibitory postsynaptic currents (sIPSCs) in a concentration-dependent manner but had no effect on glutamatergic spontaneous excitatory postsynaptic currents (sEPSCs). In contrast, for evoked synaptic responses in isolated neurons, the amplitude of both inhibitory and excitatory postsynaptic currents (eIPSCs and eEPSCs) was decreased in a concentration-dependent manner. This was associated with increases of synaptic failure rate (Rf) and paired-pulse ratio (PPR). The effect was larger for eIPSCs compared with eEPSCs. These results clearly indicate that D2O acts differently on inhibitory and excitatory neurotransmitter release machinery. Furthermore, D2O significantly suppressed GABAA receptor-mediated whole cell current (IGABA) but did not affect glutamate receptor-mediated whole cell current (IGlu). The combined effects of D2O at both the pre- and postsynaptic sites may explain the greater inhibition of eIPSCs compared with eEPSCs. Finally, D2O did not enhance or otherwise affect the actions of the general anesthetics nitrous oxide and propofol on spontaneous or evoked GABAergic and glutamatergic neurotransmissions, or on IGABA and IGlu. Our results suggest that previously reported effects of D2O to mimic and/or modulate anesthesia potency result from mechanisms other than modulation of GABAergic and glutamatergic neurotransmission.

Modulation of excitatory synaptic transmission in rat hippocampal CA3 neurons by triphenyltin, an environmental pollutant

Triphenyltin (TPT) is an organometallic compound that poses a known environmental hazard to some fish and mollusks, as well as mammals. However, its neurotoxic mechanisms in the mammalian brain are still unclear. Thus, we have investigated mechanisms through which TPT modulates glutamatergic synaptic transmission, including spontaneous, miniature, and evoked excitatory postsynaptic currents (sEPSCs, mEPSCs, and eEPSCs respectively), in a rat hippocampal CA3 ‘synaptic-bouton’ preparation. TPT, at environmentally relevant concentrations (30nM to 1μM), significantly increased the frequency of sEPSCs and mEPSCs in a concentration-dependent manner, without affecting the currents’ amplitudes. The facilitatory effects of TPT on mEPSC frequency were seen even in a Ca2+-free external solution containing tetrodotoxin. These effects were further prolonged by adding caffeine, which releases Ca2+ from intracellular Ca2+ storage sites. In glutamatergic eEPSCs evoked by paired-pulse stimuli, TPT at concentrations greater than or equal to 100nM markedly increased the current amplitude by the first pulse and decreased failure rate and pair-pulse ratio. On the other hand, both voltage-dependent Na+ and Ca2+ channels were unaffected by submicromolar concentrations of TPT. Overall, these results suggest that TPT, at environmentally relevant concentrations, affects presynaptic transmitter release machinery by directly modulating Ca2+ storage. Further, findings of this study imply that excitotoxic mechanisms may underlie TPT-induced neuronal damage.

On proportional reversed failure rate class

Motivated by the recent use of the proportional reversed failure rate in economics (rates of increase and elasticity, see Veres-Ferrer and Pavia (Stat Pap 55:275–284, 2014) and in reliability (stochastic comparisons among systems, see Khaledi et al. (J Stat Plan Inference 141:276–286, 2011), in this work, we investigate characterizations and closure properties of the decreasing proportional reversed failure rate (DPRFR) classes for continuous, nonnegative random variables. Among others, we prove that DPRFR distributions are closed under convolutions. In addition, we relate this class of distributions with the class of monotone failure rate, proportional failure rate and likelihood ratio distributions.

Combined GPS and BeiDou Instantaneous RTK Positioning

The Chinese BeiDou-2/COMPASS Navigation Satellite System has attained regional operational status and is expected to reach the same level of popularity as GPS once it has reached its full constellation. This contribution considers combined BeiDou+GPS relative code positioning and single- and multiple-frequency real-time kinematic (RTK) positioning. A combined system increases the redundancy for solving the unknown GNSS parameters and thus allows for more precise position estimates, improved reliability and robustness against failure of any of the systems. The performance is evaluated by ambiguity success rates and by comparing the estimated positions to very precise benchmark coordinates. We make use of the LAMBDA method for integer ambiguity resolution, in combination with the Fixed Failure-rate Ratio Test to validate the resolved ambiguities. The combined model will be shown to allow for improved ambiguity resolution performance and positioning robustness and accuracy over the BeiDou- and GPS-only solutions. Copyright © 2014 Institute of Navigation.