Introduction: Fecal microbiota transplantation (FMT) has become a staple in the treatment of recurrent Clostridium Difficile infections (CDI) refractory to treatment with antibiotics. Failure rate in the treatment of recurrent infections with antibiotics approaches 60%. The success of FMT in treatment of recurrent infections is related to the restoration of the gastrointestinal microbiome. Though efficacy of FMT has been reliably demonstrated in literature, little is known about the determinants of recurrence following initial FMT. Our center sought to study the efficacy and outcomes associated with FMT via colonoscope. Methods: From 2014-2016, 44 patients with recurrent CDI after failure of antibiotic regimens were recruited to undergo FMT at a single center. Demographic data, procedural data (i.e. donor stool mass, volume of donor stool transplanted) and outcomes were recorded for each patient and follow up was pursued at 1, 3, 6 and 12 months. Results: Of the 44 patients, 7 experienced recurrence between 21 and 300 days from the time of initial FMT (cure rate of 84.1%). Of those 7 patients, 5 underwent repeat FMT (from either the original or a different donor) with resolution through current follow up (cumulative cure rate of 95.5%). Of those patients who experienced recurrence, 5 were male and 2 were female. There was no significant difference in age between those who had durable resolution and those who had recurrence. The mean mass/volume of donor stool for patients with recurrence after initial FMT was 90g/349mL, whereas those who had successful initial treatment received a mean of 120g/355mL. Discussion: FMT has been demonstrated as a superior mode of treatment for recurrent CDI. We present the experience from a single center, where 44 patients underwent FMT, with an 84% cure rate after initial FMT and 95.5% cure rate following repeat FMT. Interestingly, the mean mass of donor stool in patients who experienced recurrence was notably less than in patients who experienced durable resolution. The quantity of stool required for FMT has been poorly described in literature and outcome based data is sparse. Our cohort of patients experienced cure rates comparable to what is available in current literature. However, we postulate that recurrence following initial FMT is likely multifactorial and donor stool mass/volume may be a significant variable in durable resolution.