Faecalis Counts Research Articles

An invitro comparison of antimicrobial efficacy of sodium hypochlorite gel with twin kleen irrigating solution against enterococcus feacalis

The Enterococcus faecalis (E. faecalis) is a normal inhabitant of the oral cavity and is the most commonly identified species in post treatment asymptomatic persistent endodontic diseases (both intra-radicular and extra- radicular). Its prevalence ranges from 24% to 77%. To completely eliminate microorganisms from the root canal system, combination of instrumentation and irrigation with antibacterial solutions is necessary. Hence, the purpose of this study was to evaluate and compare the antibacterial efficacy of sodium hypochlorite gel with twin kleen irrigating solution against E. faecalis.40 non-carious, single-rooted teeth with straight canals were selected and instrumented till F3 (30.06) ProTaper Universal (Dentsply Maillefer, Ballaigues, Switzerland). E faecalis was prepared and incubated in teeth and were randomly divided into four groups of 10 each, Group A – 5.25% NaOCl gel, Group B – Twin Kleen Irrigating solution, Group C – 3% NaOCl solution and Group D – Saline. Sterile paper points were used to collect the samples after irrigation and then they were transferred to blood agar culture plates to check the presence of E. faecalis. Significant statistical difference was seen between the four groups. The effect of 5.25% NaOCl gel was superior as compared to Twin Kleen irrigating solution.Under the limitations of this study, 5.25% NaOCl gel was effective in reducing E. faecalis count followed by Twin kleen irrigating solutions.

In vivo studies could not confirm in vitro prophylactic synergism between Moringa essential oil and Lactobacillus reuteri (MT180537).

Aerobic vaginitis (AV) is a recently defined vaginal recurring infection, which is treated with antibiotics. However, excessive and prolonged use of antibiotics disrupts healthy vaginal microflora and leads to the emergence of antibiotic resistance among pathogens. This situation has directed researchers to explore alternative antimicrobials. The current study describes in vitro and in vivo antimicrobial efficacy and pharmaceutical interactions between plant essential oils (EOs) and five lactic acid bacteria (LABs), isolated from the healthy vagina, against E. faecalis, one of the major etiological agents of AV. In vitro experiments confirm good antimicrobial activity of both plant EOs and cell free supernatant (CFS) from LABs. Based on high antimicrobial efficacy, Moringa essential oil (MO) was selected to determine its nature of interaction with CFS of five LAB strains. Synergism was recorded between MO and CFS of L. reuteri (MT180537). To validate in vitro findings, prophylactic responses of individual and synergistic application of MO and L. reuteri (MT180537) were evaluated in an E. faecalis (MW051601) induced AV murine model. The prophylactic efficacy was evidenced by a reduction in intensity of clinical symptoms, E. faecalis (MW051601) count per vaginal tissue along with a reduction in AV associated changes in histological markers of infection in animals receiving Moringa essential oil and L. reuteri (MT180537) alone or in combination. However, significant synergism between Moringa essential oil and L. reuteri (MT180537) could not be observed. Our data confirms the importance of in vivo experiments in deducing pharmacological interactions.

Photodynamic Therapy With Resveratrol and an Nd:YAG Laser for Enterococcus faecalis Elimination.

Enterococcus faecalis is the leading cause of endodontic treatment failures. Despite various conventional disinfection approaches, microorganisms often persist in root canals. Photodynamic therapy (PDT) is an adjunct antimicrobial strategy employing a nontoxic photosensitizer (PS) and light source. This study evaluated the antimicrobial effect of PDT using an Nd:YAG laser and resveratrol (RSV) with or without pigment, and confirmed that RSV is nontoxic as a PS. We employed laser irradiation at a 3W output power, using RSV and red pigment as the PS, on an E. faecalis bacterial solution. Subsequently, colony-forming units were quantified. The impact of RSV on osteoblasts was measured using an MTT assay. E. faecalis counts declined after laser irradiation. The combined application of laser irradiation with RSV, red pigment, or both showed a reduction compared to no irradiation and control groups without RSV and red pigment. The 50% cytotoxic concentration against osteoblast cells from mice incubated with RSV for 48 h was 162 μM. The value with RSV and laser was 201 μM and that with RSV and red pigment was 199 μM. The value with RSV, laser and red pigment was 357 μM. The combination of Nd:YAG laser irradiation and RSV as the PS with pigment was efficacious for E. faecalis elimination without inducing any toxic effects on osteoblasts. This combination holds potential as a root canal irrigation strategy.

Efficacy of Triple Antibiotic Paste and Bromelain Paste As Intracanal Medicament Against Enterococcus faecalis: An In-Vivo Study.

Introduction Microorganisms are pivotal contributors to pulp and periapical pathology, often culminating in endodontic treatment failures. The successful outcome of such treatment hinges on the effective eradication of microbial flora through various interventions, including intracanal medicaments (ICMs). However, Enterococcus faecalis (E. faecalis), a highly adaptive Gram-positive bacterium, exhibits remarkable resilience and can persist even after rigorous disinfection efforts. This study explores the efficacy of two ICMs, calcium hydroxide-based triple antibiotic paste (TAP) and a novel alternative containing bromelain, derived from pineapples, in combating E. faecalis infections in vivo. Methodology This in-vivo study was conducted at Sharad Pawar Dental College and Hospital, Wardha, India, and ethical approval was obtained from the institutional ethical committee. The sample size was calculated using the OpenEpi program (version 3.04.04, Open Source Epidemiologic Statistics for Public Health, www.OpenEpi.com), resulting in 15 participants per group. Inclusion criteria encompassed mandibular premolars with carious involvement but no prior restorations, diagnosed with necrotic or infected pulp, and no significant medical history. Exclusion criteria included systemic conditions, pregnancy, retreatment cases, recent antibiotic therapy, calcified canals, and other contraindications. After proper patient consent, access opening and chemomechanical preparation were performed, and root canals (RCs) were randomly divided into two groups: TAP and bromelain paste (BP). TAP was prepared using ciprofloxacin, metronidazole, and minocycline, while BP comprised bromelain powder mixed with saline. Both groups received intracanal medicaments, followed by temporary sealing. Microbiological samples were collected before and after treatment for analysis. Results E. faecalis count (CFU/mL) before instrumentation for the TAP group was (1.94 x 105[Formula: see text] 7.45 x 103) and for BP group was(1.97 x 105[Formula: see text] 7.70 x 103) with p-value 0.26[Formula: see text] p=0.05, so no significant difference was found between them.E. faecalis count (CFU/mL) after instrumentation for the TAP group was (7.70 x 103 [Formula: see text] 9.11 x 102) and for the BP group was(7.26 x 103 [Formula: see text] 8.43 x 102) with p-value 0.18 [Formula: see text] p=0.05, so no significant difference was found between them. However, the E. faecalis count obtained after seven days of intracanal medicament for the TAP group was (3.63 x 101 [Formula: see text] 5.60) and for the BP group was(3.13 x 101 [Formula: see text] 4.55) with p-value 0.012 < p=0.05, so a significant difference was found between them. This means that when compared with the TAP group, there was a greater amount of reduction in E. faecalis count (CFU/mL) for the BP group seven days after the placement of intracanal medicament, and this reduction was statistically significant. Conclusion This in-vivo study highlights the potential of BP as a more effective intracanal medicament against E. faecalis when compared to the conventional TAP. Bromelain's selectivity for Gram-positive bacteria and its diverse therapeutic properties make it a promising natural alternative for endodontic treatment. Further research is warranted to optimize bromelain's clinical application and assess its potential to enhance endodontic outcomes, potentially revolutionizing the field of endodontics.

Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers against a Multispecies Engineered Biofilm: An In Vitro Study.

The present study's objective was to compare the impact of CerasealR, total fill BC SealerR, Bio-C SealerR, AH Plus BioceramicR, and K-BiocerR on the elimination of a multispecies' endodontic biofilm at 3, 7 and 14 days. A total of 20 freshly extracted, caries-free premolars were prepared for the study to create dentinal disks. For the multispecies biofilm formation, Enterococcus faecalis, Proteus mirabilis, Pseudomonas aeruginosa, and Candida albicans were cultured and used to inoculate hydroxyapatite discs. After incubation, the biofilms were placed on blotting papers in petri dishes with an orthodontic bend. Different root canal sealers, including CeraSeal, total Fill BC Sealer, Bio-C Sealer, AH Plus Bioceramic, K-Biocer, and Sealite, were injected into the bend, facilitating contact with the biofilms. The samples were divided into seven groups, including a negative control. At specific intervals, 3, 7, and 14 days, 3 biofilm samples from each group were collected, diluted, and plated on Agar media for colony counting and analysis. In all tested groups, the total bacterial count significantly decreased between day 3 and 14 (p < 0.05) with no statistically significant differences among the different sealers' groups at all-time points for the total bacterial count, E. faecalis count, and P. mirabilis count. However, Sealite demonstrated the most consistent effectiveness in reducing bacterial counts across multiple categories. The sealite group was capable of decreasing the C. albicans count significantly between day 3 and day 14 (p < 0.05) in comparison with the bioceramic groups. All sealers had antibacterial activity against the multispecies biofilm between day 3 and day 14. The ascending order of sealers in terms of their effectiveness in killing bacteria, based on the provided results, is as follows: Sealite, Bio-C Sealer, AH Plus, CeraSeal, TotalFill, and K-Biocer. However, there were no statistically significant differences in the bacterial counts among the different sealer groups at any time point. The role of sealers in combating biofilm-associated infections highlights their potential clinical utility in preserving root canal health. Understanding the antimicrobial properties of these sealers is vital for informed decision-making in selecting the most effective materials for improved treatment outcomes and long-term success in endodontic procedures.

Contamination of Agricultural Soils in Some Baghdad Areas with Antibiotics Resistant Pathogenic Fecal Bacteria

Background: Early studies have shown that agricultural soil contains various types of microorganisms, especially bacteria, including coliform bacteria (Salmonella, Shigella, Klebsiella, Escherichia coli, and Enterobacter) with fecal Gram-positive bacteria like Enterococcus faecalis. Therefore, the current study aimed to investigate the contamination of Iraqi agricultural soils with pathogenic fecal bacteria (Escherichia coli and Enterococcus faecalis) and study the antibiotic sensitivity patterns of soil-isolated bacteria because it is a dangerous indicator when transmitted to humans. Methods: Soil samples were collected from six locations (farms) in the capital, Baghdad, which were AL-Jadria, AL-Latifia, Diyala River, AL-Jazera, and AL-Zafraniya (block 1 and block 2) during the study period from the end of November 2021 to August 2022; then were compared with the control samples (house garden). These bacteria were isolated by selective culture media and identified using the VITEK® 2 Compact system, and antibiotic sensitivity tests were carried out against 18 different antibiotics by the Kirby Power method. The t-test was used for the statistical analysis. Results: The bacteriological study of agricultural soil showed the presence of fecal bacteria, and this is evidence of contamination of agricultural soil samples with these bacteria. The highest E. coli count was in the AL-Latifia farm (1. 48× 103), while the highest E. faecalis count was in the Diyala River farm (2.63 × 103). The antibiotic sensitivity profile illustrated that E. coli was resistant to ampicillin, ceftriaxone, cefoxitin, piperacillin, ceftazidime, and Teicoplanin but was sensitive to the rest of the antibiotics used, while E. faecalis was only resistant to levofloxacin and linezolid and highly sensitive to the other tested antibiotics. Conclusion: The current study documented the presence of fecal coliform bacteria in studied soil samples, with markedly high resistance rates toward used antibiotics. These facts might be the result of irrigation with sewage water and the use of organic fertilizers Received:March 2023 Accepted: May 2023 Published: Oct. 2023

In vitro reduction in Enterococcus faecalis count following root canal preparation with Neolix and XP shaper rotary files

Introduction: The main goal of root canal treatment is to decrease the count of intracanal microorganisms and their byproducts. This study aimed to assess the efficacy of Neolix and XP Shaper rotary files for the reduction of Enterococcus faecalis (E. faecalis) count in the root canal system. Materials and Methods: This in vitro, experimental study evaluated 58 single-rooted single-canal teeth. The teeth were randomly divided into two experimental groups (n = 25) for root canal instrumentation with Neolix and XP Shaper files and positive and negative control groups (n = 4). All teeth were primarily instrumented with #20 K-file and #2 and #3 Gates-Glidden drills. The smear layer was removed using 17% ethylenediaminetetraacetic acid and 5.25% sodium hypochlorite. The teeth were then autoclave sterilized and the root canals in all groups, except for the negative control, were inoculated with E. faecalis. All teeth were then CO2 incubated for 24 h. Primary samples were collected. Next, the root canals in Group 1 were instrumented with Neolix, while the root canals in Group 2 were instrumented with XP Shaper. Root canals in the positive control group were only rinsed with saline. Secondary samples were collected after instrumentation. The number of colonies formed on the blood-agar was counted before and after instrumentation. Results: The negative control group showed no bacterial growth. The positive control group showed a 14.11% reduction in bacterial count. The mean percentage of reduction in bacterial colony count was 92.92% and 87.79% in the Neolix and XP Shaper groups, respectively. The difference in this regard was significant between the two experimental groups with different apical sizes (Mann–Whitney test, P = 0.02). Conclusion: Both files caused a significant reduction in E. faecalis count. However, Neolix had a superior efficacy for canal cleaning compared with XP Shaper.

Comparative Evaluation of Antimicrobial Efficacy of Calcium Hydroxide, Chlorhexidine, and Triple Antibiotic Paste in Different Combination Forms as Intracanal Medicaments against Enterococcus faecalis in Primary Teeth: An In Vivo Randomized Clinical Trial.

To compare and evaluate the antimicrobial efficacy against Enterococcus faecalis (E. faecalis) between a mix of calcium hydroxide [Ca(OH)2] powder and normal saline, a mix of Ca(OH)2 powder and 2% chlorhexidine (CHX) gluconate solution, a mix of triple antibiotic powder (TAP) and normal saline, and mix of TAP and 2% CHX gluconate solution. A total of 60 teeth were included in the study. The first sample (S1) was collected after access opening from the widest canal of the tooth by inserting sterile absorbable paper point no 20 up to the full length of the canal for 1 minute. The second sample (S2) was collected after the chemomechanical preparation and irrigation. After that, subjects were randomly divided into four groups-group I-a mix of Ca(OH)2 and normal saline; group II-a mix of Ca(OH)2 and 2% CHX; group III-a mix of TAP and normal saline; and group IV-a mix of TAP and 2% CHX. Assigned intracanal medicaments were placed in the canals, and the teeth were temporarily sealed with a temporary restorative material. On the 7th day, canals were reopened and irrigated, and a third bacteriological sample (S3) was taken out. Later, canals were filled with suitable obturating material, followed by the placement of the permanent restoration. There was a very highly significant (p < 0.005) difference in E. faecalis count in all the groups on day 7 after placement of intracanal medicament, being highest in group IV followed by group II, group III, and group I. Triple antibiotic powder (TAP) mixed with 2% CHX gluconate solution has superior antimicrobial efficacy against E. faecalis in primary teeth. Qamar S, Jayanna R, Ahuja VR. Comparative Evaluation of Antimicrobial Efficacy of Calcium Hydroxide, Chlorhexidine, and Triple Antibiotic Paste in Different Combination Forms as Intracanal Medicaments against Enterococcus faecalis in Primary Teeth: An In Vivo Randomized Clinical Trial. Int J Clin Pediatr Dent 2023;16(3):448-452.

Effect of glycolic acid as single irrigant versus sodium hypochlorite and EDTA on enterococcus faecalis count and smear layer removal in single-rooted teeth (an in vitro study)

Aim: The study aimed to compare 10% Glycolic acid as a single irrigant versus 2.5% sodium hypochlorite followed by 17% Ethylenediaminetetraacetic acid (EDTA) in terms of E. faecalis count reduction and smear layer removal in single-rooted teeth.Methodology: Thirty human extracted mandibular premolars were prepared and inoculated with E. faecalis for one week to evaluate E. faecalis count reduction. Teeth were randomly assigned to two groups (n = 15) according to the irrigation protocol used. Group I: intervention (10% Glycolic acid) and Group II: Control group (Irrigation using 2.5% NaOCl followed by 17% EDTA). After 24 hours of incubation, bacterial count reduction using colony forming units CFUs/ml was determined. For evaluating the ability of irrigants to remove the smear layer, another twenty-two mandibular premolars were chosen and randomized into two groups (n = 11). During “chemo-mechanical” preparations, the root canals were irrigated with different irrigation protocols according to each group. Then, the roots were split longitudinally in the bucco-lingual direction into two halves. A smear layer assessment was conducted on the “coronal, middle, and apical thirds” of the root canal using an Environmental Scanning-Electron Microscope (ESEM) at 1000x magnification.Results: Regarding E. faecalis count reduction, there was “no significant” difference between the two groups (p = 0.14). Regarding the ability to remove the “smear layer”, there was no significant difference between the two groups at coronal, middle and apical. While comparing smear layer scores within the same group, the “apical third” showed a significantly higher smear layer score in the two groups.Conclusion: Glycolic acid can be used as a single irrigant owing to its antibacterial efficacy and the ability to remove the smear layer.

Effect of probiotics and rice on ileal microbial count and intestinal morphometry in broiler chicken

An experiment was conducted in the Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy to find out the effect of probiotics and rice on ileal microbial count and intestinal morphometry in broiler chicken for a period of 42 days. A total of 144, day-old Ven Cobb 430 Y broiler chicks were allotted randomly to six dietary treatment groups with three replicates of eight birds each. The treatment groups were T1-standard broiler ration (SBR1), T2- SBR1 with 0.05 per cent probiotic mixture, T3- SBR1 with 0.1 per cent probiotic mixture, T4- 50 per cent of maize from SBR1 replaced by rice (SBR2), T5- SBR2 with 0.05 per cent probiotic mixture and T6- SBR2 with 0.1 per cent probiotic mixture. The dietary inclusion of rice did not affect the E. coli count and ileal pH. However the coliform count was significantly (p&lt;0.01) lower in group fed rice based diet supplemented with 0.1 per cent probiotics and the E. faecalis count was significantly (p&lt;0.01) higher in rice fed group without probiotic supplementation than corn fed group without probiotic supplementation. The probiotic supplementation at 0.1 per cent level significantly (p&lt;0.01) reduced the E. coli and coliform counts in both rice and corn based diets. The E. faecalis count and ileal pH was significantly (p&lt;0.01) reduced by probiotics at 0.05 and 0.1 per cent levels in corn based as well as rice based diets. The dietary inclusion of rice in broiler diet significantly increased (p&lt;0.01) the ileal villi height in the non-supplemented group. The ileal VH:CD ratio was significantly (p&lt;0.01) increased in rice fed groups compared to corn fed groups. The supplementation of probiotics in both rice and corn based diets significantly (p&lt;0.01) increased the villi height of duodenum, jejunum and ileum and VH: CD ratio in jejunum and ileum. It could be concluded that the dietary supplementation of probiotics at 0.1 per cent level was effective in reducing the intestinal E. coli, coliform count, E. faecalis count, ileal pH and improving the intestinal histo-morphometry. Dietary replacement of 50 per cent corn by rice had no significant effect on these parameters.

Comparison of disinfection effectiveness through manual instrumentation, rotary system and photodynamic therapy in primary molars: An in vitro study

Aim: Due to the particular anatomy of primary teeth, improvements in the materials and protocols used for disinfecting and modelling the root canal system are needed to develop a more objective and efficient approach. The aim of this in vitro experimental study was to compare the disinfection of the root canal system of primary teeth with manual, rotary and photodynamic therapy systems. Methods: Forty-eight canals of primary molar teeth were infected with a suspension of Enterococcus faecalis and divided into four groups (n = 12): group G1, composed of canals prepared with manual H-type files from the Angelus® system via the crown-down technique; group G2, treated with the Sequence of Baby Files™ rotary system by the pecking motion technique; group G3, using the G2 treatment + photodynamic therapy, with methylene blue as photosensitizer at a concentration of 0.005%, irradiated with a 660 nm diode laser for 100 seconds, 100 mW power, 10 J energy and 357 J/cm² fluence; and group G4 (negative control), which received no treatment. Samples of the canal contents were collected with sterile paper cones before and after instrumentation, diluted and seeded on BHI agar plates. The bacterial colonies formed were counted, and the results were log transformed and subjected to analysis of variance (ANOVA) and Dunnett’s test. Results: Showed a bacterial reduction in all studied groups compared to the control group. Conclusions: We concluded that the manual, rotary and photodynamic therapy systems all significantly reduced the E. faecalis count. Keywords: Deciduous Teeth; Enterococcus faecalis; Root Canal Therapy

The Antimicrobial and Antibiofilm Activity of Oregano Essential Oil against Enterococcus faecalis and Its Application in Chicken Breast.

Oregano essential oil (OEO) possesses anti-inflammatory, antioxidant, and cancer-suppressive properties. Enterococcus faecalis is a foodborne opportunistic pathogen that can be found in nature and the food processing industry. The goal of this investigation was to explore the antimicrobial action and mechanism of OEO against E. faecalis, inactivation action of OEO on E. faecalis in mature biofilms, and its application in chicken breast. The minimum inhibitory concentration (MIC) of OEO against E. faecalis strains (ATCC 29212 and nine isolates) ranged from 0.25 to 0.50 μL/mL. OEO therapy reduced intracellular adenosine triphosphate (ATP) levels, caused cell membrane hyperpolarization, increased the intracellular reactive oxygen species (ROS), and elevated extracellular malondialdehyde (MDA) concentrations. Furthermore, OEO treatment diminished cell membrane integrity and caused morphological alterations in the cells. In biofilms on stainless-steel, OEO showed effective inactivation activity against E. faecalis. OEO reduced the number of viable cells, cell viability and exopolysaccharides in the biofilm, as well as destroying its structure. Application of OEO on chicken breast results in a considerable reduction in E. faecalis counts and pH values, in comparison to control samples. These findings suggest that OEO could be utilized as a natural antibacterial preservative and could effectively control E. faecalis in food manufacturing.

Effect of Eighteeth Ultra X Ultrasonic activator device versus Syringe irrigation technique on reduction of Enterococcus Faecalis count inside the root canal space (An in-vitro study)

Effect of Eighteeth Ultra X Ultrasonic activator device versus Syringe irrigation technique on reduction of Enterococcus Faecalis count inside the root canal space (An in-vitro study)

Impact of Different Access Cavity Designs and Ni–Ti Files on the Elimination of Enterococcus faecalis from the Root Canal System: An In Vitro Study

The present research aimed to reveal the in vitro effects of different endodontic file systems utilizing different endodontic access cavity designs on the elimination of Enterococcus faecalis from root canal systems. One hundred and fifty mandibular molar teeth were separated into three groups with respect to the access cavity design: traditional endodontic cavity (TEC), conservative endodontic cavity (CEC), and truss access cavity (TAC) (n = 50). After access cavity preparation, inolulation of teeth with E. faecalis was undertaken for 21 days. Following the first sampling (S1), further division of the groups into five subgroups (n = 10) was performed: ProTaper Gold, 2Shape, TruNatomy, Reciproc Blue, and WaveOne Gold. After instrumentation, bacterial sampling (S2) was performed. Bacterial reduction was evaluated in colony-forming units/mL, and statistical analysis was carried out. The E. faecalis count decreased significantly in all groups (p &lt; 0.05). The range of mean values for a decrease in E. faecalis in log numbers was lowest in the TAC and Reciproc Blue groups, while the highest values of bacterial reduction were detected in the TEC and ProTaper Gold groups. The highest level of antibacterial action was obtained with the use of ProTaper Gold compared to the other file systems tested.

Comparative Evaluation of Enterococcus faecalis Counts on Different Tapers of Rotary System with Different Irrigating Solutions: An In Vitro Study

Introduction: The conservation of tooth structure and prevention of extrusion of obturating materials have been cited as primary advantages of minimal apical enlargements. The aim of this study is to compare the effects of different rotary file tapers and two irrigating solutions and a combination of two irrigating solutions on E. faecalis counts. In this study, 84 freshly extracted single rooted single canal human premolars were selected and enlarged to ISO #20 K-file. Materials and Methods: The samples were sterilized and inoculated with E. faecalis for 72 hours, divided into six experimental groups, and prepared with #30 nickel-titanium rotary files with 0.04 and 0.06 tapers. Group I was irrigated with 2 mL of 3% sodium hypochlorite, Group 2 was irrigated with 2 mL of 2% chlorhexidine (CHX), and Group 3 was irrigated with 2 mL of a combination of 3% sodium hypochlorite, saline, and 2% CHX irrigation after each file. Cleaning efficacy was evaluated in terms of the reduction of colony forming units. Comparison of the E. faecalis among the groups at 0.04 taper was performed using the Kruskal-Wallis test after establishing normality of variance using the Shapiro-Wilk test. A comparison of the E. faecalis between 0.04 and 0.06 taper within the group was performed using the Mann-Whitney test. Results: Group 1 (3% sodium hypochlorite) and Group 3 (3% sodium hypochlorite + saline + 2% chlorhexidine gluconate) showed no statistically significant difference between the subgroups (0.04 and 0.06 taper), whereas statistically significant difference with E. faecalis was seen with respect to Group 2 between 0.04 and 0.06 taper (2% chlorhexidine gluconate). Conclusion: The study proved the use of sodium hypochlorite, saline, and CHX in combination at both 0.04 taper and 0.06 taper gave better results with no significant change.

Comparative Effect of Various Irrigants with Herbal Formulations in Reducing Enterococcus Faecalis Count

Background: Enterococcus faecalis is associated with different forms of periradicular diseases, both primary and persistence endodontic infections. Traditional herbals have high antimicrobial, anti-inflammatory, antioxidant and biocompatible properties that make their use in dentistry more extensive. Judicious use of these herbal formulations has been found to mitigate the E.faecalis count. Aim: To compare the effect of various irrigants with herbal formulations in reducing Enterococcus faecalis count Materials and methods: Different herbal formulated irrigants were procured commercially. The test organism E.faecalis was isolated and maintained in the Nutrient Agar Slope at 4 degree C. Different concentrations of herbal irrigants were mixed with BHI agar and 50μL of the test organisms were added and poured into sterile petri dishes. Lawn culture of the test bacteria was made on the Plates and incubated at 37 degree C for 24 hrs and the colony forming units(CFU) were counted and recorded. Result: In this present study, comparing all 3 samples there is a higher reduction of count in Sample 3 (Cardamom and Tulsi mediated) than Sample 1 and Sample 2. Conclusion: Based on the results presented, it is possible to conclude that the herbal formulations represent a good reduction of Enterococcus faecalis.

Comparative antimicrobial efficacy of 2% chlorhexidine and Cupral paste along with electrophoresis in the apical region of root canals obstructed with a fractured instrument

Background and Objectives: Fracture of rotary files in the root canal system is a relatively common incident that obstructs access to the apical region and negatively affects treatment outcome. This study aimed to compare the antimicrobial efficacy of 2% chlorhexidine (CHX) and Cupral paste along with electrophoresis in the apical region of root canals obstructed with a fractured instrument. Materials and Methods: In this ex-vivo experimental study, the root canals of 72 extracted single-rooted single-canal human teeth were instrumented, sterilized, and inoculated with Enterococcus faecalis (E. faecalis). A rotary file was intentionally fractured at 3 mm from the apex. The teeth were randomly divided into 6 groups of positive control, electrophoresis with 2% CHX, electrophoresis with Cupral paste, electrophoresis alone, irrigation with 2% CHX alone, and application of Cupral paste alone. The apical 3 mm of the root canals was then cut, and the number of colony-forming units (CFUs) in this region was counted. Data were analyzed by one- way ANOVA and Tukey’s test. Results: All interventions decreased the bacterial count in the apical region. The difference in the bacterial count was significant among the 6 groups (P=0.00). Electrophoresis with Cupral paste showed superior antimicrobial efficacy than other groups, but its efficacy was not significantly higher than that of electrophoresis with 2% CHX (P&gt;0.05). Conclusion: Electrophoresis with 2% CHX and Cupral paste yielded comparably efficacy for reduction of E. faecalis count in the apical region of root canals obstructed by a fractured instrument.

Establishing in vitro and in vivo Co-culture Models of Staphylococcus epidermidis and Enterococcus faecalis to Evaluate the Effect of Topical Fluoroquinolone on Ocular Microbes.

Purpose: To establish in vitro and in vivo ocular co-culture models of Staphylococcus epidermidis and Enterococcus faecalis and to study how various concentrations of moxifloxacin affect the survival of these two endophthalmitis-causing bacteria.Methods: Standard strains of S. epidermidis and E. faecalis were used. Color detection agar plates were employed to distinguish their colonies. To establish the in vitro and in vivo co-culture models, S. epidermidis and E. faecalis were co-cultivated at different ratios for various periods. For the in vivo model, various volumes and concentrations of either a mono-culture or co-culture were inoculated into the lower conjunctival sac of rabbits. Finally, the newly developed in vitro and in vivo co-culture models were subjected to the moxifloxacin treatment to access its effect on S. epidermidis and E. faecalis.Results: When S. epidermidis and E. faecalis were cultured separately in tryptic soy broth, their growth peaked and plateaued at approximately 16 and 6 h, respectively. When they were co-cultured, the growth peak of S. epidermidis got delayed, whereas the growth peak of E. faecalis did not change. The number of E. faecalis was significantly higher in the co-culture than that in the mono-culture. Treatment with moxifloxacin in the in vitro co-culture model rapidly decreased the number of S. epidermidis cells at doses ≥ 0.125 μg/ml. In contrast, the number of E. faecalis did not change significantly up to 16 μg/ml moxifloxacin. In in vivo co-culture (at 1:1), the S. epidermidis count decreased in a pattern similar to that seen in in vivo mono-culture and was barely detectable at 24 h after inoculation. In contrast, the of E. faecalis count increased up to 16 h and then decreased. When moxifloxacin was applied (zero, one, or two times) to this model, the S. epidermidis count decreased in proportion to the number of treatments. In contrast, the E. faecalis count increased with moxifloxacin treatment.Conclusions: The in vitro and in vivo co-culture models of S. epidermidis and E. faecalis were established to determine the influence of moxifloxacin eye drops on these bacteria. The results clearly show that the moxifloxacin eye drops can make E. faecalis dominant on the ocular surface.

The antibacterial effect and the incidence of post-operative pain after the application of nano-based intracanal medications during endodontic retreatment: a randomized controlled clinical trial.

This clinical trial aimed to evaluate the effect of nano-silver and nano-calcium hydroxide intracanal medicaments (ICM) during retreatment regarding their antibacterial effect and their effect on post-operative pain and flare-ups. Sixty-nine patients scheduled for endodontic retreatment were included in this randomized clinical trial and randomly allocated to 3 equal groups (n = 23) according to the type of ICM used. The first microbial sampling (S1) representing the original microbiota was obtained after the removal of the old canal filling. After chemo-mechanical debridement, another sample (S2) was obtained representing the microbial state before ICM application. Patients were randomly allocated to receive either nano-silver (nano-Ag), nano-calcium hydroxide (nano-CH), or calcium hydroxide (CH) as ICM. Patients rated their pain pre-operatively and then after 6, 12, 24, 48, and 72h. During the second visit (7days later), the last microbial sample (S3) was obtained after removal of the ICM. Reduction of total bacterial and total E. faecalis counts and the biofilm-forming capability of the existing microbiota were determined. Results showed reduction in total bacterial count, total E. faecalis count and the biofilm-forming,capability of the existing microbiota after chemo-mechanical debridement (S1-S2) and after the application of ICM (S3-S2). However, the reduction after cleaning and shaping was significantly more pronounced (p < 0.001) compared to the effect of ICM application, with no difference between the 3 ICM (p > 0.05). Post-operative pain was significantly reduced at the 48- and 72-h intervals after the application of nano-Ag and nano-CH only (p < 0.001), with no significant difference between these two ICM (p > 0.05). The incidence of flare-ups in all groups was similar (p > 0.05). The antibacterial effect of the nano-Ag and nano-CH was equivalent to that of CH, but they contributed to better pain control. Nanoparticles may have a positive impact on post-endodontic pain.

Impact of cold atmospheric plasma on microbial safety, total phenolic and flavonoid contents, antioxidant activity, volatile compounds, surface morphology, and sensory quality of green tea powder

The effects of cold atmospheric plasma (CAP) at different voltages of 20, 22, and 25 kV for 2, 4, 6, and 8 min were investigated on microbial safety, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), volatile compounds profile, microstructure and sensory properties of green tea powder. CAP treatments effectively reduced the total microbial, mold and yeast, total coliform, Escherichia coli, and Entrococcus faecalis counts of green tea. E. coli and E. faecalis were completely deactivated in the sample exposed to plasma treatment at 25 kV for 8 min. E. coli showed higher minimum inhibitory and minimum bactericidal concentrations compared to Staphylococcus aureus. TPC, TFC and AA of green tea decreased with increases in CAP voltage and exposure time. GC–mass spectrometry identified 48 different volatile components in green tea. CAP treatment profoundly influenced the amounts of volatile compounds but the trends of changes did not follow the same pattern as the time and/or voltage of the process increased. SEM analysis showed that CAP at 20 kV for 2 or 4 min did not change the surface morphology of green tea powder while higher exposure times and voltages caused substantial ruptures in microstructure of the samples. Sensory acceptability of green tea decreased noticeably after plasma treatment at 25 kV for longer than 4 min. In conclusion, CAP treatment at 22 kV for 4 or 6 min considerably diminished microbial load of green tea powder while relatively satisfactorily preserved its TPC, TFC, AA, volatile components and sensory desirability.