Comparative Effect of Various Irrigants with Herbal Formulations in Reducing Enterococcus Faecalis Count

Abstract

Background: Enterococcus faecalis is associated with different forms of periradicular diseases, both primary and persistence endodontic infections. Traditional herbals have high antimicrobial, anti-inflammatory, antioxidant and biocompatible properties that make their use in dentistry more extensive. Judicious use of these herbal formulations has been found to mitigate the E.faecalis count. Aim: To compare the effect of various irrigants with herbal formulations in reducing Enterococcus faecalis count Materials and methods: Different herbal formulated irrigants were procured commercially. The test organism E.faecalis was isolated and maintained in the Nutrient Agar Slope at 4 degree C. Different concentrations of herbal irrigants were mixed with BHI agar and 50μL of the test organisms were added and poured into sterile petri dishes. Lawn culture of the test bacteria was made on the Plates and incubated at 37 degree C for 24 hrs and the colony forming units(CFU) were counted and recorded. Result: In this present study, comparing all 3 samples there is a higher reduction of count in Sample 3 (Cardamom and Tulsi mediated) than Sample 1 and Sample 2. Conclusion: Based on the results presented, it is possible to conclude that the herbal formulations represent a good reduction of Enterococcus faecalis.