Fecal Coliform Contamination Research Articles

Integrating One-Health Approaches: Total & Fecal Coliform Contamination from Wastewater to River Systems and Its Implications for Clean Water and Agriculture (Case Study: Garut Regency, Indonesia)

Integrating One-Health Approaches: Total & Fecal Coliform Contamination from Wastewater to River Systems and Its Implications for Clean Water and Agriculture (Case Study: Garut Regency, Indonesia)

Hubungan Jarak Jamban Terhadap Kandungan Bakteri Fecal Coliform Pada Air Sumur Gali Desa Kuranji Di Nagari Lubuk Jantan

Domestic waste that is disposed of carelessly is one of the causes of soil pollution. This research was conducted in the village of Kuranji Nagari Lubuk Jantan, the measurement result are filled in based on meansuring the distance from the latrine to the dug well using a meter. Fecal Coliform variable, the MPN (Most Probable Number) test was used on dug well water with reference to SNI 06-4158-1996 at the Andalas University Water Laboratory. Of the 31 dug well facilities, there are 31 dug well facilities (100%) that have well water with Fecal Coliform that does not meet the requirements in accordance with the 2017. Research result that: 1. Based on the proportion of fecal coliform in Kuranji village, laboratory test result on 31 dug well samples do not meet the requirements for direct consumption in RI Minister of Health Regulation No. 416/Menkes/per/IX/1990 with a percentage of 100%. 2. The potential spread of fecal coliform contamination is due to inadequate contruction. 3. Of the 31 samples of dug wells and latrines that were observed, 23 dug wells had an unsatisfactory contamination distance of 11 meters with a contamination rate of 29 with a percentage of (93.55), 2 dug wells had a satisfactory distance to the latrine of 11 meters with a percentage of 6.50%.

Antibiotic residues and microbial contamination in pasteurized whole milk intended for human consumption.

Milk contamination for human consumption is one of the biggest concerns worldwide. To prevent milk contamination, it is important to implement sustainable production practices that ensure animal health and guarantee veterinary drugs have been used properly. This study aimed to detect antibiotic residues and microbial contamination in commercially available pasteurized whole milk intended for human consumption. We conducted a cross-sectional study on all brands of pasteurized milk (n = 17) for human consumption in Medellín, Colombia, from February 30 to April 30, 2022. Six milk samples of each brand were collected every 15 days, resulting in 102 samples. IDEXX SNAPduo™ ST Plus test (IDEXX Laboratories Inc, Maine, USA) was used to detect cephalosporins residues to detect beta-lactam and tetracyclines. We detected mesophilic aerobic bacteria and coliforms using Chromocult Coliform Agar® (Merck KGaA, Darmstadt, Germany) and Plate-Count Agar® (Merck KGaA), respectively. Beta-lactam residues were found in 24.4% of the brands. No tetracyclines or cephalosporins were detected. Mesophilic aerobic bacteria and coliform contamination were detected in 42.6% and 12.8% of the brands, respectively. No fecal coliform contamination was detected. This study demonstrated the presence of antibiotic residues and microbial contamination in commercially available pasteurized whole milk intended for human consumption in the study area, highlighting its potential public health implications.

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Assessment of faecal contamination of water is a key factor in assessing the quality and safety of food. It also reveals the level of hygiene adopted by food handlers in the course of preparation and serving of such foods. This study assessed the risk of microbial contamination of water used by food vendors in Owerri munipality, Imo State Nigeria between January-December 2019 with an emphasis on total coliforms and the faecal coliforms/ faecal indicator bacteria (FIB) – Escherichia coli (E. coli) and thermotolerant coliforms (TTC). One hundred and thirty water samples collected across three vending types (street vendors, high level and low level restaurants) were evaluated for microbial contamination using standard methods. Data obtained was statistically analyzed with p-value set at 0.05 significance level. The result identified Enterobacter spp. and Klebsiella spp. The result showed that 48.46% (63) of the 130 water samples examined were contaminated with coliform bacteria, of which 20 (22.22%) samples had faecal coliform bacteria, representing 10.77% of the entire water samples examined. Of the 64 samples from water used for food preparation, 12.50% (8) had faecal coliform bacteria as against 9.09% (6) of 66 samples collected from water offered to customers for drinking. The result showed that 116 (89.23%) of the 130 water samples recorded no faecal coliform contamination, hence they were considered excellent based on the guidelines for determination of faecal contamination of water. Two samples had faecal coliform counts of 1-3 per 100 ml of water accounting for 1.54% of water samples, which is considered Satisfactory. The result revealed suspicious (4-9 per 100 ml) coliform count in 9 (6.92%) water samples, while three water samples (2.31%) had ≥10 count and are classified as unsatisfactory. The result revealed that 89.23% (116) of the examined water samples were of ‘no risk’ to the public, which was significantly higher than 8.46% (11) and 2.31% (3) recognized as being of low and intermediate risk respectively (p <0.05). High and very high risk water samples were not encountered in the study. This trend in qualification of risk of water samples reflected in the two types of water usages, and the observed differences was however not statistically significant (p>0.05).

Risk of Faecal Contamination of Water Used by Food Vendors in Owerri Munipality, Imo State Nigeria

Assessment of faecal contamination of water is a key factor in assessing the quality and safety of food. It also reveals the level of hygiene adopted by food handlers in the course of preparation and serving of such foods. This study assessed the risk of microbial contamination of water used by food vendors in Owerri munipality, Imo State Nigeria between January-December 2019 with an emphasis on total coliforms and the faecal coliforms/ faecal indicator bacteria (FIB) – Escherichia coli (E. coli) and thermotolerant coliforms (TTC). One hundred and thirty water samples collected across three vending types (street vendors, high level and low level restaurants) were evaluated for microbial contamination using standard methods. Data obtained was statistically analyzed with p-value set at 0.05 significance level. The result identified Enterobacter spp. and Klebsiella spp. The result showed that 48.46% (63) of the 130 water samples examined were contaminated with coliform bacteria, of which 20 (22.22%) samples had faecal coliform bacteria, representing 10.77% of the entire water samples examined. Of the 64 samples from water used for food preparation, 12.50% (8) had faecal coliform bacteria as against 9.09% (6) of 66 samples collected from water offered to customers for drinking. The result showed that 116 (89.23%) of the 130 water samples recorded no faecal coliform contamination, hence they were considered excellent based on the guidelines for determination of faecal contamination of water. Two samples had faecal coliform counts of 1-3 per 100 ml of water accounting for 1.54% of water samples, which is considered Satisfactory. The result revealed suspicious (4-9 per 100 ml) coliform count in 9 (6.92%) water samples, while three water samples (2.31%) had ≥10 count and are classified as unsatisfactory. The result revealed that 89.23% (116) of the examined water samples were of ‘no risk’ to the public, which was significantly higher than 8.46% (11) and 2.31% (3) recognized as being of low and intermediate risk respectively (p <0.05). High and very high risk water samples were not encountered in the study. This trend in qualification of risk of water samples reflected in the two types of water usages, and the observed differences was however not statistically significant (p>0.05).

Biochar and flow interruption control spatio-temporal dynamics of fecal coliform retention under subsurface drip irrigation

Applying wastewater in subsurface drip irrigation helps address water shortage in arid and semi-arid environments. Microbial contamination may result, but soil amendments such as biochar could help protect soil and water resources. To improve understanding, this study investigated spatio-temporal dynamics of fecal coliform retention in a biochar-treated soil under subsurface drip irrigation. Two dripper discharges rates of 2 and 4 l hr−1 containing fecal coliforms (3.99 ± 1.4 × 105 CFU ml−1), three maximum allowable depletion levels (MAD; 30, 50, and 70%), and three biochar levels (0, 0.5 and 1%, w/w) were used. The lysimeters were filled with biochar-treated soil and had drippers located at 20 cm depth. Three irrigations were performed for either Q, and soil samples were taken after each irrigation at different times at the lysimeter centre at 5, 15, 25, and 50 cm depths and at the edge at 20 cm depth (labeled 20 L). Soils containing biochar had much greater recovered coliforms from soil than the unamended controls. The greatest retention was at 15 and 25 cm depth (within 5 cm of the dripper) for 1% biochar, with the recovered cells about 70% for 2 l hr−1 discharge and 60% for 4 l hr−1 discharge. The greatest concentration occurred immediately after irrigation, but over 10 days the number of coliforms gradually decreased and inactivated. Therefore, the coliform residence time, soil depth, and biochar rate all influenced coliform retention if the discharge rates and MADs were considered. We recommend slower subsurface dripper rates with high MAD and biochar amendment to minimize fecal coliform contamination from subsurface field wastewater irrigation.

Sustenance of Himalayan springs in an emerging water crisis

Springs are a significant source of high quality and perennial freshwater supply for remote communities and sustain rich biodiversity and ecosystems in the Himalayas. About 60-70% of the Himalayan population directly depends on springs to meet their domestic and livelihood needs. Despite that, decline in approximately 60% of low discharge springs have been reported in the last couple of decades. In addition, nitrates and faecal coliform contamination linked to septic tanks, open defecation, and fertiliser application have been reported. A high degree of urbanization with 500 growing townships and 8-10 large cities has further threatened the sustenance of these vital resources, causing a severe water crisis in the Himalayas. Spring rejuvenation can enhance water access and livelihoods and help achieve several sustainable development goals (SDGs). However, multiple challenges hinder the success of such initiatives. A fundamental limitation is the poor understanding of complex groundwater (spring) systems and their interactions with human societies. This review identified crucial knowledge gaps by synthesizing available knowledge on springs and revival efforts from peer-reviewed journals and reports by practitioners and governing bodies. The review also highlights the limitations of spring revival approaches and recommends future management options. There is a critical lack of comprehensive data as a large research on the Himalayan spring systems results from small-scale spring centric studies focussing primarily on hydrology. In contrast, the impacts of hydrogeology, ecology, socio-economics and developmental activities on springs are less explored. Lack of scientific inputs on the hydrogeological regime and limited support by the state is a barrier to scaling spring rejuvenation programs. Long term monitoring, location-specific mapping of local hydrogeological and socio-economic settings at aquifer scale and collaborations among different stakeholders are essential to facilitate holistic knowledge development on spring systems and successful spring revival. The authors recommend ensuring sustenance by recognizing the value of springs in the mainstream programs and policies and develop appropriate management framework for the management of spring systems.

Assessment of Groundwater and Surface-Water Resources of Gushkara in Purba Bardhaman (West Bengal, India) using the Water Quality Index

Survival of life on earth indispensably depends on water. Population explosion and its consequences resulted in the usable water sources being unusable in many regions. Regular monitoring of water quality becomes necessary to protect public health. The present study aimed to evaluate the water quality status of various water resources of Gushkara (West Bengal, India). The result showed that the status of the water quality index (WQI) of the studied surface-water sources is ‘poor’ or ‘very poor’ (ranges 50.582 - 87.969) whereas the groundwater and municipal water supply are rather safer (ranges 15.804 - 36.210). There is also an indication of faecal coliform contamination in surface-water sites. Overall water quality is better in winter than other two seasons. Some parameters in surface-water crossed the recommended level while some reached near that level. So immediate attention is necessary to rectify the situation.

Water sanitation, hygiene and the prevalence of diarrhea in the rural areas of the delta region of Myanmar.

Myanmar is an agriculture-based country with 70% of the total population residing in rural areas. Around half of the total population in Myanmar has to consume water from unimproved sources. The prevalence of diarrhea due to contaminated drinking water is high even in urban areas. The urban community may expect the provision of municipal water supply in the near future if the current revolution against military dictatorship succeeds. However, the rural areas have less or no chance to get quality water because of a lot of other prioritized tasks. Household water treatment is encouraged to be implemented as one of the national water safety plans for rural water supply in Myanmar. This study explored the diarrhea prevention awareness of the rural community using a questionnaire survey. The microbial quality parameters of drinking water sources were also examined. Fecal coliform contamination was detected in all examined drinking water sources. A significant association was found between drinking untreated water and the occurrence of diarrhea. The percentage of people who applied the diarrhea preventive measures was low. Even if they knew how to prevent the disease, very few people applied the measures in reality. Therefore, measures to cause behavioral change should be initiated, together with awareness raising, to promote diarrhea prevention in the community.

Bacteriological quality of household drinking water in North Gondar Zone, Ethiopia; a community-based cross-sectional study

Ethiopia is one of the developing countries where only 52% of its population have access to safe drinking water. To determine the level of faecal contamination in drinking water, the E. coli has been found to be the most specific indicator. The purpose of this study was to determine household bacteriological drinking water quality and its associated factors in rural North Gondar Zone Ethiopia. A community-based cross-sectional study was conducted in rural parts of North Gondar Zone from April to June, 2016. Water samples from water storage at household level were taken using standardized water sampling techniques to determine the level of faecal contamination. Face-to-face interview using structured questionnaires and checklist were used to identify the associated factors. A total of 736 households participated in this study. The prevalence of positive faecal coliform in the households is found to be 72.6% (95%C.I; 69.4–75.8). In multivariate analysis; occupational status [AOR; 0.35, 95%; CI (0.16,0.77)], income [(AOR);0.45, 95%; CI (0.3, 0.68)], type of solid waste disposal habit [AOR; 0.16, 95%; CI (0.36, 0.66)], source of water [AOR; 0.42, 95%; CI (0.28, 0.65)], shortage of water [AOR; 2.8, 95%; CI (1.88, 4)] were variables identified as predictors for faecal coliform contamination of water in the household. There is high level of faecal contamination of household drinking water in North Gondar Zone. Occupational status, income, solid waste disposal habit, sources of drinking water, and shortage of water were predictor for presence of faecal coliform contamination in household drinking water.

Impact of microbial multi-metal and broad spectrum antibiotic tolerance in urban SW (Adi Ganga, Kolkata) on adjacent groundwater: A future threat

One hundred and sixty six bacterial strains were isolated from a highly polluted location of Adi Ganga surface water (SW, n = 144), groundwater (GW, n = 22) system for their multi metal and antibiotic resistance study. A BOD(5) of 45 mg/L and ORP of −368 mV with faecal coliform contamination (>1800/100 ml) was noted for SW along with elevated levels of Zn (228 μg/L) and Mn (396 μg/L). However, no faecal coliform could be detected in GW sample. Multi-metal tolerance was observed to be a common property among the strains isolated. Resistance to seven or more heavy metals was prevalent among 70% of the SW bacterial population. Remaining 30% comprised isolates from both SW and GW resistant to 2–6 metals. Bacterial resistance to metals was highest for Pb followed by Cr, Mo, Ni, Hg, Cd, As, Cu, Co, Zn. Muti antibiotic resistance profiling indicated very high resistance towards ampicillin, chloramphenicol, aerosporin and streptomycin whereas the population was considerably sensitive to ciprofloxacin and gentamycin. High MAR index of 0.8 (SW) and 0.3 (GW) is a threat to the health of mankind and a possible indication of origin of multidrug resistant bacterial infections. Correlation in heavy metal and antibiotic resistance suggested their natural co-selection. Acquisition of such genetic elements by disease causing pathogenic microbes might confer resistance to a broad spectrum of antibiotics. Contamination of GW with such multi-metal and antibiotic resistant strains may spread the resistance property to human microflora.

Prevalence of coliform bacterial contamination in cat drinking water in households in Thailand.

Background and Aim:Bacterial contamination of drinking water is a leading cause of gastrointestinal infections. Cats may be at risk of water contamination from feces due to poor sanitation and hygiene. The objectives of the present study were to (1) evaluate the prevalence of coliform bacteria in cat drinking water and (2) identify possible risk factors leading to contamination.Materials and Methods:Fifty-five drinking water samples were collected from water containers used by cats (median age [range]: 5 years [8 months-15 years]) at their home. Using a sterile syringe, 50 mL water was collected directly from water containers. The water samples were stored in coliform enhancement media for 24 h and then submitted for bacterial culture.Results:The prevalence of fecal coliform contamination of cat drinking water was 67.27% (37/55; 95% confidence interval: 53.29-79.32%). There was no significant difference in the prevalence of coliform bacterial contamination of drinking water by age or gender of the cat or by water container type. However, bacterial contamination differed significantly between shorthaired cats and longhaired cats when comparing Escherichia coli (9/44 [20.45%] vs. 8/11 [72.72%], p<0.001) and Enterobacter spp. (16/44 [36.36%] vs. 9/11 [81.82%], p=0.007). For water that had been in a container longer than 12 h, there were significantly more contaminated tap water samples (16/19 [84.21%]) than contaminated processed water samples (9/17 [52.94%], p=0.047).Conclusion:Coliform contamination in cat drinking water is common and occurs more often in households with longhaired cats. Drinking water for cats should be changed every 12 h, especially for households using tap water.

Effect of Chlorination on Microbiological Quality of Effluent of a Full-Scale Wastewater Treatment Plant.

The evaluation of effluent wastewater quality mainly relies on the assessment of conventional bacterial indicators, such as fecal coliforms and enterococci; however, little is known about opportunistic pathogens, which can resist chlorination and may be transmitted in aquatic environments. In contrast to conventional microbiological methods, high-throughput molecular techniques can provide an accurate evaluation of effluent quality, although a limited number of studies have been performed in this direction. In this work, high-throughput amplicon sequencing was employed to assess the effectiveness of chlorination as a disinfection method for secondary effluents. Common inhabitants of the intestinal tract, such as Bacteroides, Arcobacter and Clostridium, and activated sludge denitrifiers capable of forming biofilms, such as Acidovorax, Pseudomonas and Thauera, were identified in the chlorinated effluent. Chloroflexi with dechlorination capability and the bacteria involved in enhanced biological phosphorus removal, i.e., Candidatus Accumulibacter and Candidatus Competibacter, were also found to resist chlorination. No detection of Escherichia indicates the lack of fecal coliform contamination. Mycobacterium spp. were absent in the chlorinated effluent, whereas toxin-producing cyanobacteria of the genera Anabaena and Microcystis were identified in low abundances. Chlorination significantly affected the filamentous bacteria Nocardioides and Gordonia, whereas Zoogloea proliferated in the disinfected effluent. Moreover, perchlorate/chlorate- and organochlorine-reducing bacteria resisted chlorination.

Heavy Metal and Microbial Contaminants of Some Vegetables Irrigated With Goo Reservoir Water, Navrongo, Ghana

Globally, the safety of vegetables for consumption is becoming an increasing concern to consumers because of the risk associated with eating of vegetables contaminated with heavy metals and microbial organisms. An assessment of the extent of microbial contamination and also levels of heavy metals and the risk associated with the consumption of the vegetables irrigated with polluted Goo reservoir water in the Navrongo municipality was carried out. Site A used the channel flooding irrigation method whilst site B used watering cans for watering during the latter part of the dry season when the pressure of the water is low. A total of 128 vegetables samples were taken for microbial and heavy metals determination each. Samples of leafy vegetable and fruit vegetables were randomly taken from the two sites for microbial and heavy metal analysis. The reservoir was divided to North, South, East and West and water samples taken from each location. The concentrations of heavy metals in the reservoir exceeded the Food and Agricultural Organization (FAO) recommended levels of metals in water for irrigation. Site B recorded the highest microbial counts likewise heavy metal contaminants in the sampled vegetables. Levels of cadmium in the vegetables exceeded the World Health Organization/FAO permissible levels. Copper (Cu) had the highest concentration in both sites. Faecal Coliform (FC) levels in the vegetables were above the International Commission on Microbiological Specifications for Food (ICMSF) allowable limits. The high quantities of Total Coliform, Faecal Coliform, E. coli, helminthes eggs and salmonella contamination of the vegetables indicate high risk of getting diseases through the consumption of these vegetables. The hazard quotient of all the metals exceeded one in both sites except Zinc (Zn). The hazard index (HI) of heavy metals studied was above one in both sites, indicating they could have adverse health effect to human life. The analysis showed there was significant difference in microbial counts and levels of heavy metals in the vegetables in the two different sites. The consumers of these vegetables were at risk of contracting water-borne diseases like typhoid fever, cholera among others and also a high risk of heavy metal poisoning especially from cadmium.

Arsenic Mobilization Process in Shallow Aquifer of Bengal Delta Plain: A Field Scale Study to Identify the Role of Coliform Bacteria

In Bengal Delta Plain (BDP), shallow aquifer (&lt;50 m) is often contaminated with Arsenic (As). The phenomenon is wide spread in nature thought the BDP notable in Nadia district of west Bengal. The present study highlights a primary screening of As, Fe, MPN and FC in monitored shallow wells. The study designed for two different sites (site-A, High As and site-B Low As area). The water quality monitoring results suggest that high As concentration (Range- 103-171 μgL-1) has been noticed in site A when compared with site B (range-53-99 μgL-1). In sites A, the Fe concentration is high and low in site B. The correlation study (r2) between arsenic and iron are also determined. The value of r2is 0.94 for site A and 0.73 for site B. The water quality results suggest that the nature of the monitor aquifer is anoxic in nature with low Eh, DO absent and low NO3- and SO4+. Major anion is HCO3- (376 mgL-1) followed by Cl- (28 mgL-1). However chloride concentration is largely varying in the monitored tube well. Microbial study (MPN &amp; FC count) also indicates some relationship among MPN (r2-0.32) and Fe (r2 -0.24). However the relationship is scatter when As concentration is low. The linear trended has also obtained when both As, MPN and FC are high. The physical observation of plate count (Color reaction in Chromo colt Agar) has also been observed. This is a clear indicator of fecal coli form contamination. The study indicates that the microbial mobilization of As is the key factor for enrichment of As in ground water. The possible sources of the microbes are local land-use pattern (notable pit-latrine). Finally, the study highlights the role of coli forms bacteria (Both facultative and non-facultative) are wide spread in shallow rural aquifer of Bengal. Thus microbial process possibly enriches arsenic in shallow ground water.

Evaluating Contamination Level of Raw and Roasted Nuts Distributed in Commercial Markets in Mazandaran Province, Iran

BACKGROUND:Raw and roasted nuts are one of the most popular snacks consumed in Iran but mishandling and poor storage conditions and practices may lead to promote the growth of microorganisms and foodborne intoxications and infections. OBJECTIVES:The purpose of this study was to investigate the microbial quality of raw and roasted (salted and unsalt- ed) nuts, distributed in commercial markets in Mazandaran province, Iran. METHODS:The moisture content, total colony counts, total coliform, fecal coliform, Staphylococcusaureus, and fungal contamination were evaluated according to procedures of Iran national standards. RESULTS: The moisture content of raw and roasted hazelnut, pistachio, almond, and cashew nut samples were above the standard limits. The moisture content of the raw peanuts was within the standard approved limit (maximum 9%). But the moisture content of the roasted peanut was higher than the permissible limit of 5%. For yeasts and moulds, except for all almond samples and 56% of cashew nut samples, all products tended to be loaded with unsatisfactory limits. The total viable count was within the standard limits in hazelnut, almond, and cashew nut samples (less than 4log cfu/gr). Satisfactory limits of coliforms (lower than 1log cfu/gr) were observed in all almond samples, 91.6% of hazelnut, and 16.7% of pistachio samples. No fecal coliforms were detected in samples. S.aureus contamination was observed in 6.5% of hazelnut, 8% of almond, 25% of cashew nut, and 33% of peanut samples. CONCLUSIONS: According to the findings, almond and hazelnut showed the least, and pistachio and peanut samples showed the highest microbial contamination.

Anthropogenic markers for source tracking of fecal contamination in Bayou Lafourche: a major drinking water source in Southeast Louisiana, USA

Abstract Bayou Lafourche, which is the sole drinking water source for 300,000 people in Louisiana, has failed to consistently meet its designated use criteria set by the Louisiana Department of Environmental Quality (LDEQ). This resulted in a total maximum daily load to be imposed on the Bayou by the Environmental Protection Agency (EPA). These designated use water quality criteria include fecal coliform (FC) levels for drinking water source, primary contact recreation, and secondary contact recreation. The goal of this study was to identify and enumerate anthropogenic nonpoint source FC contamination from malfunctioning home sewage systems in the Bayou's watershed. Thirty-four sites along the Bayou were selected for the study. Samples were analyzed for optical brightener ratios, FC CFU/100 mL (mFC), Escherichia coli, and three human markers, including human polyomavirus BK, the Archaeon Methanobrevibacter smithii, and the human-associated Brevibacterioides HF 183 eubacteria. Frequencies of sites with all three anthropogenic molecular markers are considered positive for human fecal contamination. This study provided data to address the problem of malfunctioning on-site sewage systems in the Bayou Lafourche watershed.

The pristine nature of river Ganges: its qualitative deterioration and suggestive restoration strategies.

The river Ganges, the National Heritage, and the lifeline of millions of Indians, unfortunately, ranked the second most polluted rivers of the world in 2017. This review reveals the current trends of the water quality of the Ganges assessed around 36 stretches during 2012-2016, to indicate an improvement around 6 (16.7%), deterioration around 14 (38.9%), and non-significant changes around 16 (44.4%) stretches. An increase in dissolved oxygen and a decrease in biochemical oxygen demand were observed at six stretches (Devprayag [S5], Rishikesh upstream [S7], Varanasi upstream [S19], Mokama upstream [S25], Mokama downstream [S26], and Munger [S27]). The total and fecal coliform contamination decreased at seven stretches (Rudraprayag [S2 and S3], Devprayag [S5 and S6], Rishikesh [S7], Varanasi upstream [S19], and Munger [S27]) due to improved hygienic conditions, but it increased subsequently at eight stretches (Haridwar [S8], Kanpur [S15], Raibareili [S16], Prayagraj [S17 and S18], Patna [S24], Berhampore [S30], and Serampore [S31]) due to improper defecation and mass bathing during 2007-2016. Dissolved oxygen level declined significantly, and biochemical oxygen demand increased (> 3 ppm), alarmingly at places receiving heavy untreated sewage water. The water quality of the Ganges was good up to Rishikesh, because of an undisrupted flow of the uncontaminated water from the higher altitudes (≥ 372 m) with higher forest cover, lower temperatures (< 21 °C), and higher dissolved oxygen (≥ 8.5 ppm) and due to the dissolution of antipathogenic chemical constituents of the medicinal herbs, pollutant degrading alkaline phosphatase, and bacteriophages. The present review is a systematic collection of data on river pollution, its scientific analyses, and its relationship with 6Ps (namely population, poverty, pollution, precipitation, plantation, and periodicity). Not only that, but the river water restoration measures have also suggested through the novel interlinked water working groups for implementing integrated water management strategies.

Molecular characterization and antimicrobial resistance profile of fecal contaminants and spoilage bacteria that emerge in rainbow trout (Oncorhynchus mykiss) farms.

Fecal contaminants are a major public concern that directly affect human health in the fish production industry. In this study, we aimed to determine the fecal coliform, spoilage bacteria, and antimicrobial-resistant bacterial contamination in rainbow trout (Oncorhynchus mykiss) farms. Fish were sampled from rainbow trout farms that have a high production capacity and are established on spring water, stream water, and dammed lakes in six different regions of Turkey. A total of seven Enterobacter subspecies, two strains of Pseudomonas spp., and one isolate each of Morganella and Stenotrophomonas were characterized based on biochemical and molecular methods, including the 16S rRNA and gyrB housekeeping gene regions. The sequencing results obtained from the 16S rRNA and gyrB gene regions were deposited in the GenBank database and compared with isolates from different countries, which were registered in the database. Resistance to 10 different antimicrobial compounds was determined using the broth microdilution method, and molecular resistance genes against florfenicol, tetracycline, and sulfamethoxazole were identified by PCR. All detected resistance genes were confirmed by sequencing analyses. E. cloacae, E. asburiae, Pseudomonas spp., S. maltophilia, and M. psychrotolerans were identified using the gyrB housekeeping gene, while isolates showed different biochemical characteristics. All isolates were found to be phenotypically resistant to sulfamethoxazole, and some isolates were resistant to tetracycline, florfenicol, amoxicillin, and doxycycline; the resistance genes of these isolates included floR, tetC, tetD, and tetE. We showed that fecal coliforms, spoilage bacteria, and antimicrobial resistant bacteria were present in farmed rainbow trout, and they pose a threat for human health and must be controlled in the farming stage of fish production.

Bacteriological Quality of the Mangrove Oyster (Crassostrea rhizophorae) in the Swamp of Mestizo - Cordoba (Colombia)

Objectives: To determine the bacteriological quality of the mangrove oyster and its aquatic environment, such as a risk factor in public health, through the consumption of these products from the natural environment. Materials and Methods: Data were collected about 60 samples of oysters and the surrounding water, between June and November 2004. Using the indirect method of counting multiple fermentation tubes, we estimated the concentration of bacteria indicators of total and fecal coliform contamination. The indicators of the bacteriological quality of the oysters presented a minimum of ≤ 3 NMP.100 mL-1 and a maximum of 46 × 104 MPN.100 mL-1 of total coliforms, fecal coliforms showed a seasonal variation in concentrations between ≤3 NMP.100 mL-1 and 2 × 103 MPN.100 mL-1 . Findings: The analysis of water samples recorded a range of concentration of coliforms from ≤ 3 NMP.100 mL-1 to 3.9 × 105 NMP.100 mL-1 and for fecal coliforms it ranged from ≤ 3 NMP.100 mL-1 to 7 × 103 MPN.100 mL-1 . This study suggests that there exists a directly proportional relationship between the bacteriological quality of the mangrove oyster Crassostrea rhizophorae and the water quality of the environment, by its status filtering, which contributes a factor of risk to public health, the consumption of this food, especially if the consumption is done in a direct way. Applications/recommendations: the potentiality of the Bay of Mestizo, Colombia, for the development of a sustainable aquaculture of the mangrove oyster (Crassostrea rhizophorae), requires baseline studies on various environmental and social aspects which will lay the basis for the socio-economic development of this region. Keywords: Molluscs, total coliforms, fecal coliforms, sustainable aquaculture, public health.