Objectives: The aim of the study was to investigate the reliability and validity of the Turkish version of the Mental Fatigue Scale (MFS) in healthy Turkish population.
 Methods: This study was held in Hacettepe University, Faculty of Physical Therapy and Rehabilitation between April and August 2019. A total of one hundred thirty-two healthy participants aged between 18-50 years were recruited. Reliability was investigated using test-retest reliability. The internal consistency of MFS was calculated using Cronbach's alpha coefficient. The validity of the MFS was assessed by comparing the MFS score with the 36-item Short Form Health Survey (SF-36) and Checklist Individual Strength Questionnaire (CIS-T) scores. Pearson correlation coefficient was used to evaluate validity.
 Results: The test–retest reliability of the MFS were excellent in healthy Turkish population (ICC: 0.91, with a 95% confidence interval of 0.88–0.94). The scale had high internal consistency (Cronbach’s α 0.86). For the validity, the correlations between the MFS and the total/subscales of CIS-T scores and SF-36 scores were good (p
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