The U.S. Hispanic population has grown substantially over the past 50 years, and simultaneously, the number of U.S. medical schools and medical school faculty has increased. This study examines the trends for Hispanic faculty in clinical and basic science departments at U.S. medical schools. Method This retrospective, cross-sectional observational study analyzed data from the Association of American Medical Colleges Faculty Roster for faculty at all LCME-accredited medical schools from 1973 to 2022. Proportions of faculty were compared according to department, sex, rank, and Hispanic status using a 2-independent-sample t-test and simple linear regressions. Results The percentage of Hispanic medical school faculty increased from 1.7% in 1973 to 5.4% in 2022, with similar rates of increase in basic science and clinical departments. In 2022, departments with the highest representation of Hispanic faculty included family medicine and obstetrics and gynecology. The departments with the lowest representation of Hispanic faculty were orthopedic surgery and otolaryngology. In 2022, only 1.5% and 3.1% of all full professors were Hispanic females and males, respectively. Conclusion There was a minimal increase in the percentage of Hispanic faculty in medical schools over the last 50 years, and the rank was disproportionally higher at the Assistant Professor level, particularly for females. The combination of a rapid increase in the U.S. Hispanic population and a slow increase in Hispanic faculty has resulted in Hispanic medical school faculty being more underrepresented in academic medicine in 2022 than in 1973.