Facultad De Medicina Research Articles

Rapid proliferation and differentiation of a subset of circulating IgM memory B cells to a CpG/cytokine stimulus in vitro

Event Abstract Back to Event Rapid proliferation and differentiation of a subset of circulating IgM memory B cells to a CpG/cytokine stimulus in vitro Andrés C. Vásquez1, Manuel A. Franco1* and Juana Angel1* 1 Pontificia Universidad Javeriana, Instituto de Genética Humana, Facultad de medicina, Colombia Human circulating IgM+ memory B cells (CD27+IgM+ (mBc IgM)) are not fully characterized. Therefore, many aspects of their biology and composition remain to be elucidated. Currently, it is unclear if there are functional and/or phenotypic differences between IgM+ mBc, naïve Bc, or switched mBc. Also, it is unclear if there are differences among IgM+ mBc subsets. When compared to naïve Bc, lower frequencies of IgM+ mBc expressed CD40, BAFF-R, CD21, CD73 and IL21-R, but higher frequencies of them expressed CD95, CD11c, CD43, TLR9, PD-1, CD122 and CD45RO. When compared to switched mBc, IgM+ mBc showed higher frequencies of cells expressing BAFF-R, CD40, PD-1, IL21-R, TLR9 and CD122, but lower frequencies expressing CD95 and CD73. Purified IgM+ mBc stimulated in vitro with CpG, IL-2, IL-6, and IL-10 for 4 days displayed higher frequencies of cells that proliferated and acquired the phenotype of antibody secreting cells, compared to naive Bc and switched mBc. Besides, with this same stimulus, a low frequency of IgM+ mBc switched isotype in vitro. The analysis of the distribution of RV-specific mBc among circulating IgM+ mBc population allowed to differentiate two IgM+ mBc subsets: IgMhi IgDlo (IgMhi) and IgMlo IgDhi (IgMlo); RV-specific mBc are enriched in the IgMhi subset. In conclusion, in response to a CpG and cytokine based polyclonal stimulus a higher frequency of the circulating IgM+ mBc population differentiates and proliferates, compared to naïve Bc and switched mBc. The RV-specific mBc distribution suggests that IgMhi and IgMlo may be subsets with differences in function and phenotype. Acknowledgements We would like to thank the subjects that participated in this study for their generosity, the Radiotherapy Unit, Centro Javeriano de Oncología, and the Hemocentro Distrital for providing samples. Keywords: memory B cell, circulating memory B cell, IgM, IgG, CpG Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Adaptive Immunity Citation: Vásquez AC, Franco MA and Angel J (2015). Rapid proliferation and differentiation of a subset of circulating IgM memory B cells to a CpG/cytokine stimulus in vitro. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00338 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Apr 2015; Published Online: 15 Sep 2015. * Correspondence: Dr. Manuel A Franco, Pontificia Universidad Javeriana, Instituto de Genética Humana, Facultad de medicina, Bogotá, Bogotá, Colombia, mafranco@javeriana.edu.co Dr. Juana Angel, Pontificia Universidad Javeriana, Instituto de Genética Humana, Facultad de medicina, Bogotá, Bogotá, Colombia, Jangel@javeriana.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Andrés C Vásquez Manuel A Franco Juana Angel Google Andrés C Vásquez Manuel A Franco Juana Angel Google Scholar Andrés C Vásquez Manuel A Franco Juana Angel PubMed Andrés C Vásquez Manuel A Franco Juana Angel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Determination of anti-Mycobacterium tuberculosis IgM and IgG antibodies in individuals in contact with patients with active tuberculosis.

Determination of anti-Mycobacterium tuberculosis IgM and IgG antibodies in individuals in contact with patients with active tuberculosis.

Naturally-ocurring biflavonoids modulate macrophage response in vitro and are atheroprotective in vivo

Naturally-ocurring biflavonoids modulate macrophage response in vitro and are atheroprotective in vivo

AN ORAL-SALMONELLA CANCER IMMUNOTHERAPY INDUCES A TUMOR-SPECIFIC IMMUNE RESPONSE IN LIVER AND LIVER DRAINING LYMPH NODES

AN ORAL-SALMONELLA CANCER IMMUNOTHERAPY INDUCES A TUMOR-SPECIFIC IMMUNE RESPONSE IN LIVER AND LIVER DRAINING LYMPH NODES

Microparticles forming immune complexes in Rheumatoid Arthritis patients could be associated with the altered phenotype of classical and intermediate monocyte subsets

Microparticles forming immune complexes in Rheumatoid Arthritis patients could be associated with the altered phenotype of classical and intermediate monocyte subsets

Searching for prognostic factors in Common Variable Immunodeficiency based on epidemiological and immunological parameters

Searching for prognostic factors in Common Variable Immunodeficiency based on epidemiological and immunological parameters

Whole platelets induces macrophage programming toward a repair profile characterized by high IL-10 production

Whole platelets induces macrophage programming toward a repair profile characterized by high IL-10 production

Global transcriptome analysis of alveolar macrophage from tuberculosis patients and non-infected controls

Global transcriptome analysis of alveolar macrophage from tuberculosis patients and non-infected controls

Allogeneic inducible regulatory T cells generated with retinoic acid allow the generation of mixed chimerism via nonmyeloablative conditioning in mice models

Allogeneic inducible regulatory T cells generated with retinoic acid allow the generation of mixed chimerism via nonmyeloablative conditioning in mice models

IgG response to different stage-specific antigens of Mycobacterium tuberculosis

IgG response to different stage-specific antigens of Mycobacterium tuberculosis

Serum and mucosal ST2 content as monitoring markers of Ulcerative Colitis outcome

Serum and mucosal ST2 content as monitoring markers of Ulcerative Colitis outcome

Overexpression of Tn Antigen induced by a hypoxia-mimetic cobalt cloride in JEG-3 choriocarcinoma cellular line

Event Abstract Back to Event Overexpression of Tn Antigen induced by a hypoxia-mimetic cobalt cloride in JEG-3 choriocarcinoma cellular line Ramiro Rios1, Juan G. Maldonado-Estrada2, Juan C. Quintana3 and Julio C. Bueno1* 1 Assistant professor, Physiology and Biochemistry, Colombia 2 Faculty of Agricultural Sciences, School of Veterinary Medicine, Colombia 3 Universidad Cooperativa de Colombia- Sede Medellin, Facultad de Medicina, Colombia Background: Cell invasion and placenta development involve variations of oxygen supply that supports phenotypic changes of tumoral and trophoblast cells, including the glycocalix and membrane glycoprotein expression. Methods: JEG-3 and the extravillous trophoblastic cell line HTR8/Svneo were incubated for 12, 24 and 48 h with 150 μM CoCl2 (cobalt cloride) . A panel of multiple lectins labeled with FITC (Datura stramonium agglutinin- DSA, Helix pomattia agglutinin-HPA, Maackia amurensis agglutinin-MAA, Sambucus nigra agglutinin-SNA, Peanut agglutinin-PSA) was used to determine glycotopes of N- and O-glycoproteins using confocal microscopy and flow cytometry. Protein extracts from these cells were assessed for lectin blot analysis and enzymatic digestion with PNGase. nvasion assays of HTR8/Svneo cells were determined using Matrigel-coated 5 μm pore size polycarbonate membrane Transwell inserts and blocking N-glycosylation with tunicamycin and castanospermine. Results: After 48 h of treatment with CoCl2, an increase the expression of Tn antigen and N-acetyl-galactosamine in JEG-3 cells was visible by lectin-binding assays with HPA and DBA respectively (P < 0,001). Furthermore, increased expression of the terminal sialic acid linked to galactose (α2-6 and α2-3) was observed in glycoproteins 60-70 kD long. The percentage of invasion observed in HTR8/Svneo cells was reduced by blocking N-glycosylation with tunicamycin and castanospermine (P < 0,05). Acknowledgements 1Reproduction Group, School of Medicine, Programa de sostenibilidad 2015-2016, Universidad de Antioquia. Medellín, Colombia. 2Centauro Group, School of Veterinary Medicine, Universidad de Antioquia. Medellín-Colombia. 3Center for the Study of Biological Systems, School of Medicine, Universidad Cooperativa de Colombia. Medellín, Colombia. Departamento Administrativo de Ciencia y Tecnología – COLCIENCIAS, Proyecto RC728-2013, Comité Nacional para el Desarrollo de la Investigación – CONADI, Universidad Cooperativa de Colombia. References Kim, Y. J. and A. Varki (1997). "Perspectives on the significance of altered glycosylation of glycoproteins in cancer." Glycoconj J 14(5): 569-576. Kim, Y. S., H. S. Yoo and J. H. Ko (2009). "Implication of aberrant glycosylation in cancer and use of lectin for cancer biomarker discovery." Protein Pept Lett 16(5): 499-507. Kobata, A. and J. Amano (2005). "Altered glycosylation of proteins produced by malignant cells, and application for the diagnosis and immunotherapy of tumours." Immunol Cell Biol 83(4): 429-439. Keywords: Tn antigen, Hypoxia-Inducible Factor 1, alpha Subunit, N-glycosylation, O-glycosylation, Sialic acid (Neu5Ac) Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Immunology of reproduction Citation: Rios R, Maldonado-Estrada JG, Quintana JC and Bueno JC (2015). Overexpression of Tn Antigen induced by a hypoxia-mimetic cobalt cloride in JEG-3 choriocarcinoma cellular line. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00334 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 May 2015; Published Online: 15 Sep 2015. * Correspondence: Prof. Julio C Bueno, Assistant professor, Physiology and Biochemistry, Medellin, Antioquia, 050010, Colombia, jcesarbs@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ramiro Rios Juan G Maldonado-Estrada Juan C Quintana Julio C Bueno Google Ramiro Rios Juan G Maldonado-Estrada Juan C Quintana Julio C Bueno Google Scholar Ramiro Rios Juan G Maldonado-Estrada Juan C Quintana Julio C Bueno PubMed Ramiro Rios Juan G Maldonado-Estrada Juan C Quintana Julio C Bueno Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

CYTOKINE SECRETION BY ENDOMETRIAL STROMAL HUMAN CELLS FROM MENSTRUATION AND DECIDUA STROMAL HUMAN CELLS FROM PLACENTAS COLLECTED OF VAGINAL AND CAESAREAN DELIVERIES.

CYTOKINE SECRETION BY ENDOMETRIAL STROMAL HUMAN CELLS FROM MENSTRUATION AND DECIDUA STROMAL HUMAN CELLS FROM PLACENTAS COLLECTED OF VAGINAL AND CAESAREAN DELIVERIES.

A Recombinant Bacillus Calmette-Guérin (BCG) vaccine promotes protective humoral immunity against the human respiratory syncytial virus

A Recombinant Bacillus Calmette-Guérin (BCG) vaccine promotes protective humoral immunity against the human respiratory syncytial virus

Contribution of FcγReceptors to Human Respiratory Syncytial Virus Pathogenesis and to the Impairment of T Cell Activation by Dendritic Cells

Event Abstract Back to Event Contribution of FcγReceptors to Human Respiratory Syncytial Virus Pathogenesis and to the Impairment of T Cell Activation by Dendritic Cells Roberto S. Gómez1*, Bruno A. Ramirez1, Pablo F. Céspedes1, Kelly M. Cautivo1, Sebastián Riquelme1, 2, Carolina Prado1, Pablo Gonzalez1 and Alexis M. Kalergis1, 2, 3 1 Millennium Institute on Immunology and Immunotherapy. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Chile 2 INSERM U1064, France 3 Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina. Pontificia Universidad Católica de Chile, Chile Human respiratory syncytial virus (hRSV) is the leading cause of infant hospitalization related to respiratory disease. hRSV infection produces abundant infiltration of immune cells into the airways, which combined with an exacerbated pro-inflammatory immune response can lead to significant damage to the lungs. hRSV re-infection is extremely frequent suggesting that hRSV may have evolved molecular mechanisms to interfere with host adaptive immunity. Infection with hRSV can be reduced administrating a humanized neutralizing antibody against the viral fusion protein in high-risk infants. Although neutralizing antibodies against hRSV block the infection of airway cells, here we show that both, BMDCs and lung DCs remain susceptible to infection by antibody-opsonized virus. We observed that such process is mediated by Fcγ receptors expressed on the surface of DCs. Remarkably, we also observed that in the absence of hRSV-specific antibodies FcγRIII knockout mice displayed significantly less cellular infiltration in the lungs after hRSV infection, as compared to wild-type mice suggesting a harmful IgG-independent role for this receptor in hRSV disease. Taken together, our findings suggest a key role for FcγRs on DC infection and subsequent immune responses to both free and IgG opsonized-hRSV, in which FcγRIII plays a relevant part in the development of hRSV pathogenesis. Acknowledgements This work was supported by funding from the Millennium Institute on Immunology and Immunotherapy from Chile (P09/016-F to AMK), La Région Pays de la Loire through the “Chaire d’excellence” program to AMK and Grant “Nouvelles Equipes-nouvelles thématiques” (AK), INSERM CDD grant, the ECOS France-Chile grant, FONDECYT no 1070352, FONDECYT no 1050979, FONDECYT no 1040349, FONDECYT no 1100926, FONDECYT no 1110397, FONDECYT no 1100971, FONDECYT no 1110604 and BMRC CTU06. RG, BR, PC, SR and KC are CON Keywords: Human respiratory syncytial virus, Fcγ receptors, Dendritic Cells, Palivizumab, neutralizing antibodies, immune complexes. Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Infectious and parasitic diseases Citation: Gómez RS, Ramirez BA, Céspedes PF, Cautivo KM, Riquelme S, Prado C, Gonzalez P and Kalergis AM (2015). Contribution of FcγReceptors to Human Respiratory Syncytial Virus Pathogenesis and to the Impairment of T Cell Activation by Dendritic Cells. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00175 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 May 2015; Published Online: 14 Sep 2015. * Correspondence: Mr. Roberto S Gómez, Millennium Institute on Immunology and Immunotherapy. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Santiago, Chile, rsgomezj@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Roberto S Gómez Bruno A Ramirez Pablo F Céspedes Kelly M Cautivo Sebastián Riquelme Carolina Prado Pablo Gonzalez Alexis M Kalergis Google Roberto S Gómez Bruno A Ramirez Pablo F Céspedes Kelly M Cautivo Sebastián Riquelme Carolina Prado Pablo Gonzalez Alexis M Kalergis Google Scholar Roberto S Gómez Bruno A Ramirez Pablo F Céspedes Kelly M Cautivo Sebastián Riquelme Carolina Prado Pablo Gonzalez Alexis M Kalergis PubMed Roberto S Gómez Bruno A Ramirez Pablo F Céspedes Kelly M Cautivo Sebastián Riquelme Carolina Prado Pablo Gonzalez Alexis M Kalergis Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

B cell abnormalities in a mouse model of SLE

Event Abstract Back to Event B cell abnormalities in a mouse model of SLE Camila Fuentes1, Natalia Crisostomo1, María A. Gleisner2, 3, Mario Rosemblatt1, 4 and Maria R. Bono1* 1 Laboratorio de Inmunología, Facultad de Ciencias, Universidad de Chile, Chile 2 Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Chile 3 Instituto Milenio de Inmunología e Inmunoterapia, Universidad de Chile, Chile 4 Fundación Ciencia & Vida, Chile Systemic lupus erythematosus (SLE) is an autoimmune disease of unknown etiology characterized by the activation of autoreactive T and B cells, autoantibody production and immune complex deposition causing damage in multiple tissues. B cells play a critical role in the development of SLE. Besides the production of auto-antibodies, it has been described that B cells have the ability to produce cytokines and present antigens, which allows them to have different roles during the immune response. Using a spontaneous autoimmune disease setting, (NZWxNZB) F1 (BWF1) mice, that develops SLE at the age of 6 months, characterized by high proteinuria levels and serum autoantibody titers, we analyzed different B cell subpopulations such as regulatory and memory populations through flow cytometry (FACS) using specific B cell markers. Important differences were seen on the regulatory B cells populations, plasma cells and B10 cells which were increased on spleen of BWF1 aged autoimmune mice. We also studied the effect of dendritic cells (DCs) in vivo over B cells and their participation in the development of the disease. Two doses of DCs from BWF1 aged autoimmune mice were transferred onto BWF1 young pre-autoimmune mice. Two months after the adoptive transfer of DCs, mice were sacrificed and B cells were studied by FACS analysis. Our results showed that the adoptive transfer of DCs from BWF1 aged autoimmune mice into BWF1 young pre-autoimmune mice promoted an accelerated and exacerbated humoral response comparable to that seen on the normal development of SLE. Thus, treatment with DCs from BWF1 aged autoimmune mice caused significant changes in the phenotype and distribution of B cells from BWF1 young pre-autoimmune mice, similar to what we see on BWF1 aged autoimmune mice, indicating that the interaction between B cells and DCs maybe determining in the development of the disease. Acknowledgements Funded by FONDECYT 1100557, PFB-16. Keywords: Lupus Erythematosus, Systemic, B cells, Dendritic Cells, Autoimmune Diseases, Autoantibodies Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Autoimmunity Citation: Fuentes C, Crisostomo N, Gleisner MA, Rosemblatt M and Bono MR (2015). B cell abnormalities in a mouse model of SLE. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00207 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Apr 2015; Published Online: 14 Sep 2015. * Correspondence: Prof. Maria R Bono, Laboratorio de Inmunología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile, mrbono@uchile.cl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Camila Fuentes Natalia Crisostomo María A Gleisner Mario Rosemblatt Maria R Bono Google Camila Fuentes Natalia Crisostomo María A Gleisner Mario Rosemblatt Maria R Bono Google Scholar Camila Fuentes Natalia Crisostomo María A Gleisner Mario Rosemblatt Maria R Bono PubMed Camila Fuentes Natalia Crisostomo María A Gleisner Mario Rosemblatt Maria R Bono Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Modulation of chronic inflammation by the mineralocorticoid receptor during experimental autoimmune encephalomyelitis.

Modulation of chronic inflammation by the mineralocorticoid receptor during experimental autoimmune encephalomyelitis.

Effect of Dexamethasone, LPS, or INFγ on the production of TNFα, IL-12, IL-6, IL-10, CXCL8, CXCL10, and CLC3, by bovine macrophages infected in vitro with Mycobacterium avium paratuberculosis.

Effect of Dexamethasone, LPS, or INFγ on the production of TNFα, IL-12, IL-6, IL-10, CXCL8, CXCL10, and CLC3, by bovine macrophages infected in vitro with Mycobacterium avium paratuberculosis.

Assessing the ability of two specific, immunodominant Taenia solium antigens to differentiate non-infected from cysticercotic subjects

Event Abstract Back to Event Assessing the ability of two specific, immunodominant Taenia solium antigens to differentiate non-infected from cysticercotic subjects Yuliet M. Diaz1, Gladis Fragoso1, Edda Sciutto1, Julio C. Carrero1, Ada N. Martínez2, Juan Pedro Laclette1 and Raúl J. Bobes1* 1 Universidad Nacional Autónoma de México, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Mexico 2 Universidad Nacional Autónoma de México, Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Mexico INTRODUCTION: Human and porcine cysticercosis are infectious diseases associated to poverty and poor sanitary conditions. Cysticercosis is a severe public health issue and causes great economic losses at the rustic pork rearing industry in Mexico and other developing countries. Many variables can influence the current Immunodiagnostic methods and alter the assay sensitivity and specificity. This has prevented the development of a suitable diagnostic test for a highly specific diagnosis. OBJECTIVE: This work is aimed to find recombinant antigens or derivative peptides suitable to develop a more sensitive and specific diagnostic test for human and porcine cysticercosis. METHODS: Based on the amino acid sequence of two antigens identified in a previous study as specific for porcine cysticercosis (annexin B1 and cAMP-dependent kinase), peptides with the highest antigenicity rates were identified by bioinformatic tools. These peptides were evaluated for their suitability as antigen source in a cysticercosis ELISA, using serum samples from cysticercotic and non-cysticercotic pigs as antibody source. Additionally, the coding sequences for the selected antigens were cloned in a prokaryotic system to evaluate the diagnostic usefulness of the entire protein. RESULTS: Seven peptides with the highest antigenic index for annexin B1 and 12 peptides for cAMP-dependent kinase were selected. A series of ELISA experiments led us to identify those peptides that were most recognized by antibodies in pig serum samples. For annexin B1, 3 peptides out of 7 showed higher recognition, while 5 out of 12 peptides showed higher recognition for cAMP-dependent kinase. The cloning, expression, and purification of the recombinant proteins annexin B1 and cAMP-dependent kinase were also performed. CONCLUSIONS: The antigens evaluated in this work, annexin B1 and cAMP-dependent kinase include linear peptides which might be good candidates to be used in a diagnostic test for cysticercosis. Acknowledgements This work is supported in part by grants from PAPIIT-UNAM: IN228609-3 and CONACyT: 61334. Keywords: Cysticercosis, diagnosis, Antigenicity, Specific antigens, Taenia solium Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Infectious and parasitic diseases Citation: Diaz YM, Fragoso G, Sciutto E, Carrero JC, Martínez AN, Laclette J and Bobes RJ (2015). Assessing the ability of two specific, immunodominant Taenia solium antigens to differentiate non-infected from cysticercotic subjects. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00191 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Apr 2015; Published Online: 14 Sep 2015. * Correspondence: PhD. Raúl J Bobes, Universidad Nacional Autónoma de México, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, México, D. F., Mexico, rbobes@biomedicas.unam.mx Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yuliet M Diaz Gladis Fragoso Edda Sciutto Julio C Carrero Ada N Martínez Juan Pedro Laclette Raúl J Bobes Google Yuliet M Diaz Gladis Fragoso Edda Sciutto Julio C Carrero Ada N Martínez Juan Pedro Laclette Raúl J Bobes Google Scholar Yuliet M Diaz Gladis Fragoso Edda Sciutto Julio C Carrero Ada N Martínez Juan Pedro Laclette Raúl J Bobes PubMed Yuliet M Diaz Gladis Fragoso Edda Sciutto Julio C Carrero Ada N Martínez Juan Pedro Laclette Raúl J Bobes Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

IFNγ effect in the induction of the Neutrophil Extracellular Traps (NETs) in Chronic Granulomatous Disease patients

IFNγ effect in the induction of the Neutrophil Extracellular Traps (NETs) in Chronic Granulomatous Disease patients