ABSTRACTRoot system is an important factor for crop productivity under water- and nutrient-limited environments. A pot study was conducted to evaluate root system response of three Thai rice varieties (Pathumthani 1, RD57, RD41) under three cultivation methods (dry direct seeding [DDS], wet direct seeding [WDS], transplanting [TP]) and three levels of alternate wetting and drying irrigation (–5, –15, –30 kPa). A second pot experiment examined the effect of potassium (K) rates (0, 80, 120, 160 kg ha–1) on root system response of the same varieties under DDS and TP subjected to alternate wetting and drying at –5 kPa. Pathumthani 1 was more tolerant to moisture stress; RD57 and RD41 showed an inconsistent response to moisture-deficit conditions. Rice plant under TP was more sensitive to moisture stress; rice plant performed better under DDS even at the highest soil moisture stress of –30 kPa. K application at the rate of 120 kg ha–1 as basal under DDS was optimum for root system development of Pathumthani 1, while RD57 and RD41 had higher actual root length at the same K rate regardless of cultivation methods. Alternate wetting and drying up to –30 kPa could be safely applied for the three tested varieties. The performance of root system of Pathumthani 1 was better under DDS method of cultivation. K fertilization at 120 kg ha–1 can be recommended as optimum rate under water-saving cultivation techniques for the three tested varieties.