To establish a reference system for assessing the anteroposterior (A-P) position of the subnasal and lower-facial soft tissues for whole facial harmony. Forty Asian and 40 Caucasian females with attractive profiles were selected as the "attractive" samples, with "ordinary" samples for comparison. Each profile was analyzed, and comparisons were made to reveal the interracial commonalities and differences. Esthetically essential parameters were established. An averaged attractive profile for each race was created by digital morphing and then modified into 30 variations based on combined variations of the esthetically essential parameters. Assessments were performed to investigate the esthetic ranges. A-P position of the subnasal and lower-facial landmarks harmonized with the forehead for female profile esthetics. In addition to balanced soft tissue subnasale (sSn)- and soft titssue pogonion (Pos)-to-forehead A-P relations, harmonizing lower-facial soft tissues to sSn was indispensable for profile attractiveness. sSn-to-glabella, Pos-to-glabella, and Pos-to-sSn A-P relations were esthetically essential. Perceived by orthodontists, the attractive Asian female profiles had sSn-to-glabella A-P relations ranging from 0.5 mm to 4.5 mm, Pos-to-sSn from -9.0 mm to -5.5 mm, and Pos-to-glabella from -8.5 mm to -1.0 mm. Compared with Asians, the attractive Caucasian female profiles had more anteriorly and widely distributed sSn relative to the forehead, wider ranges of Pos-to-sSn A-P relations, and more prominent chins. A reference system comprising sSn-glabella, Pos-sSn, and Pos-glabella horizontal distances was constructed for facial profile analysis. This system could aid treatment planning for surgical or orthopedic repositioning of the maxilla and chin.