Introduction: Lip augmentation is a frequently requested facial cosmetic procedure. The use of AlloDerm cosmetically has been documented since 1994, with clinical observations of some degree of resorption. The purpose of this prospective study was to objectively and subjectively assess the degree of labial change with AlloDerm implantation and its long-term clinical stability. Materials and Methods: Nineteen patients were treated with AlloDerm lip augmentation between January 1999 and October 1999. These patients were then followed up at 3, 6, and 12 months after surgery with a combination of physical evaluation, patient questionnaires, photographs, and lateral cephalometric radiographs. The data obtained were then evaluated objectively and subjectively. Objective analysis included computer analysis of photographs and lateral cephalometric radiographs. Subjective analysis included patient questionnaires and visual photographic assessment by professional independent evaluators. Results: Of the 19 patients initially enrolled in the AlloDerm lip study, 74% were seen on 3-month follow-up, 79% on 6-month follow-up, and 58% at 12-month follow-up. At 3 months, the compiled National Institutes of Health computer-analyzed photographic data showed an overall mean increase in the collective vermilion surface area of 16% on frontal and 39% on profile from the preoperative status. By 1 year, the overall mean increase in vermilion surface area was 8% on frontal and 36% on profile. The compiled lateral cephalometric radiographic data showed an overall mean increase in lip projection of 16% in the upper lip and 7% in the lower lip at 3 months. However, by 1 year, the overall mean increase in lip projection from preoperative status had dropped to 6% in the upper lip and 2% in the lower lip. Preoperatively, only 16% of the patients perceived their lips as looking about right or younger; this improved to 91% at 1 year. Independent evaluators viewed 54.5% of the patient's lips as looking about right or younger before surgery, as compared to 82% at 1 year. Conclusions: The objective results clearly indicate that there is substantial mean labial volumetric loss from 3 to 6 months after lip augmentation with AlloDerm. From 6 to 12 months, further volumetric loss is less substantial, with more stable clinical results. Despite documented resorption, there is an overall improvement in the subjective perception at all assessed points by both the patients and independent evaluators.