F1 Treatment Research Articles

Effect of different concentrations of soil and foliar applied zinc, boron and iron fertilizers on seedling growth, chlorophyll content and productivity of chickpea seedlings under semi-arid environment

The effectiveness of zinc (Zn), boron (B), and iron (Fe) is reduced in semi-arid regions, which can lead to a deficiency of these nutrients and inhibit chickpea productivity. In this work, three field experiments were executed over two years where soil and foliar applications of Zn, B, and Fe were carried out, including controls (Zn0, B0, and F0), soil application at 4.125 kg/ha (Zn-1, B1, and F1) and 8.25 kg/ha (Zn-2, B2, and F2), and foliar spay at 0.3% at flowering initiating (Zn-3, B3, and F3) and one week after flowering initiation (Zn-4, B4, and F4, respectively). The results indicate that the deficiency of these nutrients inhibited chickpea growth and yield, leading to a reduction in the pigment contents. Nonetheless, soil and foliar application of Zn, B, and Fe significantly improved growth, chlorophyll contents, and yield, showing a dose-dependent effect. The best results were recorded for Zn-3, B2, and F2 treatments which significantly (P<0.05) increased shoot length (20.96-85.19%), root length (42.85-93.65%), shoot fresh (23-76%) and root fresh weight (45-90.32%), compared with the control treatment. Chlorophyll parameters, including chlorophyll a and b contents, showed similar trends. Zn-3, B2, and F2 treatments significantly increased biological and grain yields, which were associated with higher values of the number of pods per plant and the number of seeds per pod. In a nutshell, we suggest that Zn foliar application at 0.3% at flowering initiation and soil application of B and Fe at 25 kg/ha are beneficial for improving the growth, pigment content, and overall productivity of chickpea.

Alleviative Effect of Exogenous Application of Fulvic Acid on Nitrate Stress in Spinach (Spinacia oleracea L.)

Salt stress could be a significant factor limiting the growth and development of vegetables. In this study, Fulvic Acid (FA) (0.05%, 0.1%, 0.15%, 0.2%, and 0.25%) was applied under nitrate stress (150 mM), with normal Hoagland nutrient solution as a control to investigate the influence of foliar spray FA on spinach growth, photosynthesis, and oxidative stress under nitrate stress. The results showed that nitrate stress significantly inhibited spinach growth, while ROS (reactive oxygen species) accumulation caused photosystem damage, which reduced photosynthetic capacity. Different concentrations of FA alleviated the damage caused by nitrate stress in spinach to varying degrees in a concentration-dependent manner. The F3 treatment (0.15% FA + 150 mM NO3−) exhibited the most significant mitigating effect. FA application promoted the accumulation of biomass in spinach under nitrate stress and increased chlorophyll content, the net photosynthetic rate, the maximum photochemical quantum yield of PSII (Photosystem II) (Fv/Fm), the quantum efficiency of PSII photochemistry [Y(II)], the electron transport rate, and the overall functional activity index of the electron transport chain between the PSII and PSI systems (PItotal); moreover, FA decreased PSII excitation pressure (1 − qP), quantum yields of regulated energy dissipation of PSII [Y(NPQ)], and the relative variable initial slope of fluorescence. FA application increased superoxide dismutase, peroxidase, and catalase activities and decreased malondialdehyde, H2O2, and O2− levels in spinach under nitrate stress. FA can enhance plant resistance to nitrate by accelerating the utilization of light energy in spinach to mitigate excess light energy and ROS-induced photosystem damage and increase photosynthetic efficiency.

Growth and Yield of Lentil as Influenced by Zinc and Boron Management

A field experiment was conducted at Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh, during the period from November 2021 to March 2022 to evaluate the influence of micronutrients management on growth and yield of two lentil varieties viz., BARI Masur-8 (V1) and Binamasur-8 (V2) and 6 micronutrients management (no fertilizer - F1, recommended fertilizer dose (RFD) (40-90-40-55 Kg ha−1 of urea-TSP-MoP-Gypsum) with 10 Kg ha−1 ZnSO4 as basal - F2, RFD with zinc (Zn) as foliar spray - F3, RFD with Zn and boron (B) as foliar spray - F4, 50% RFD + 50% cow dung (5 t ha−1) - F5 and 50% RFD + 50% cow dung + Zn and B as foliar spray - F6). No significant effect had found for variety except 1000-seed weight but micronutrients management along with interactions significantly effects on all studied parameters of lentil. The higher 1000-seed weight (34.16 g) was recorded in BARI Masur-8. The highest plant height (37.09 cm), number of branches plant−1 (7), dry weight of root plant−1 (159.17 mg), dry weight of shoot plant−1 (3319.33 mg) and number of pods plant−1 (62) was observed at F6 treatment. The highest 1000-seed weight (31.68 g) were recorded at F2 treatment. The highest weight of nodules plant−1 (10.33 mg at 60 DAS), seed yield (2.62 t ha−1), straw yield (2.12 t ha−1) and biological yield (4.74 t ha−1) were found at F3 treatment. The highest plant height (38.45 cm), number of branches plant−1 (7), number of pods plant−1 (63), seed yield (2.91 t ha−1) and harvest index (58.65%) were recorded at V2F6 whereas the highest weight of nodules plant-1 (26 mg), straw yield (2.22 t ha−1) and biological yield (5.02 t ha−1) were found at V1F3 but the highest 1000-seed weight (34.87 g) at V1F1. The highest number of seeds pod−1 (1.83) was found at V2F1. So, two varieties showed similar performance on yield. Foliar application of Zn has the potentiality to increase seed yield. The variety Binamasur-8 with 50% RFD + 50% cow dung + Zn and B (F6) as foliar spray could bring maximum yield (2.91 t ha−1) of lentil followed by treatment F2, F3, and F4. Bangladesh Agron. J. 2024, 26(2): 34-40

Comparison of different fertilization rates on yield, evapotranspiration, and water use efficiency of sweet corn under drought-salinity stresses

The present study investigated the effects of three fertilization (N-P2O5-K2O) rates (F1: 240-100-120 kg ha-1, F2: 192-80-96 kg ha-1, F3: 154-64-77 kg ha-1) coupled with four irrigation practices (Control: C, irrigated at the 100% field capacity, Drought: D, irrigated 60% of C, Saline: S, irrigated at the 100% field capacity, Drought and saline: D+S, irrigated 60% of S) on sweet corn yield, evapotranspiration (ET), water use efficiency (WUE), and shoot fresh-dry weights. The obtained results depicted that the grain yield at D, S, and D+S treatments decreased by 24.2%, 46.6%, and 62.0%, respectively, relative to the C treatment. Moreover, grain yield at the F3 condition was reduced by 45.3% compared to the F1 condition. Additionally, the highest ET (330.7 mm) and yield (74.0 g) was achieved with F1×C treatment. The F2 and F3 treatments reduced WUE by 17.9% and 31.5%, respectively, compared to the F1 treatment. The highest reduction in yield, ET, WUE, and shoot fresh-dry weights was found at D+S irrigation treatment under all fertilization conditions. The tallest plants were observed in the F1×C treatment, being 24.0%, 33.5%, and 43.2% taller than plants in the F1×(D+S), F2×(D+S), and F3×(D+S) treatments, respectively. Under F3 conditions, exposing sweet corn plants to single or combined salinity and drought stress remarkably degraded the growth ability of the plants, and therefore, it is not economical and sustainable cultivation for agriculture. Finally, cultivation of sweet corn plants under individual or combined drought-salinity stress is not recommended due to the high reduction in grain yield.

The effect of organic and mineral fertilizers on the fruit yield Cucurbita pepo L.

The experiment was carried out in the vegetable field of the Department of Horticulture and Landscape Engineering, College of Agriculture, University of Karbala - Al-Husseiniyah District during the fall semester 2023-2024 in sandy soil according to a randomized completely block design (RCBD). The experiment included 19 treatments and three repetitions. Vermicompost tea was added to the soil at three levels (4, 8 and 12) liter ha-1, and humic extract was added at three levels as well (6, 12 and 18) liter ha-1. It was added to the soil in three batches, and three levels of NPK mineral fertilizer (25%, 50%, and75%) of the fertilizer recommendation with compared to a treatment to which the full fertilizer recommendation(100%) NPK)Comparison (,was applied in three stages to investigate the impact of vermicompost tea humic acid and mineral fertilizer (NPK) on the quality of zucchini squash The results showed a significant effect of the highest concentration of vermicompost tea and mineral fertilizer 75% on the characteristics of fruit length and fruit weight, as treatment F12 excelled by giving it the highest rate of the mentioned characteristics, amounting to 18.90 cm and 210.90 g fruit -1, and treatment F10 recorded the highest rate of fruit number characteristic of 5.63 fruit-1. The results also showed that the F3 treatment was superior in terms of fruit diameter, reaching 4.132 cm. We conclude from this that it is preferable to divide the organic and mineral fertilizers into batches, and it is possible to use quality organic fertilizer with a third of the amount of mineral fertilizer, that is, reducing mineral fertilizers and replacing them with organic fertilizers.

Investigating the influence of varied ratios of red and far-red light on lettuce (Lactuca sativa): effects on growth, photosynthetic characteristics and chlorophyll fluorescence.

Far red photon flux accelerates photosynthetic electron transfer rates through photosynthetic pigments, influencing various biological processes. In this study, we investigated the impact of differing red and far-red light ratios on plant growth using LED lamps with different wavelengths and Ca1.8Mg1.2Al2Ge3O12:0.03Cr3+ phosphor materials. The control group (CK) consisted of a plant growth special lamp with 450 nm blue light + 650 nm red light. Four treatments were established: F1 (650 nm red light), F2 (CK + 730 nm far-red light in a 3:2 ratio), F3 (650 nm red light + 730 nm far-red light in a 3:2 ratio), and F4 (CK + phosphor-converted far-red LED in a 3:2 ratio). The study assessed changes in red and far-red light ratios and their impact on the growth morphology, photosynthetic characteristics, fluorescence characteristics, stomatal status, and nutritional quality of cream lettuce. The results revealed that the F3 light treatment exhibited superior growth characteristics and quality compared to the CK treatment. Notably, leaf area, aboveground fresh weight, vitamin C content, and total soluble sugar significantly increased. Additionally, the addition of far-red light resulted in an increase in stomatal density and size, and the F3 treatments were accompanied by increases in net photosynthetic rate (Pn), transpiration rate (Tr), intercellular CO2 concentration (Ci), and stomatal conductance (Gs). The results demonstrated that the F3 treatment, with its optimal red-to-far-red light ratio, promoted plant growth and photosynthetic characteristics. This indicates its suitability for supplementing artificial light sources in plant factories and greenhouses.

The antibacterial activity test of salt gel face mask with chitosan extract against propionibacterium acnes and staphylococcus aureus bacteria as antiacne preparations

Abstract Salt is a collection of chemical compounds with the main content in the form of NaCl. The mineral content in salt provides health functions for the body, one of which is acne. Salt gel face mask with chitosan extract has the potential to be used as an antibacterial (antiacne) preparation. The purpose of this study was to determine the effective antibacterial activity and concentration of salt used as an antiacne preparation against Propionibacterium acnes and Staphylococcus aureus bacteria in salt gel face masks. The research method used was a laboratory scale experimental method with a completely randomized design (CRD) consisting of 5 treatments and 4 repetitions. The treatment consists of a gel face mask with the addition of a salt concentration of 0%, 10%, 15%, 20% and 25%. The main parameters of this study were antibacterial activity, while the supporting parameters included pH, homogeneity, viscosity, organoleptic and SEM-EDX analysis. The results showed that the addition of different salt concentrations to the salt gel face mask had an effect on the antibacterial activity of the salt gel face mask. The best formulation is in the F4 treatment with a salt concentration of 25%.

Influence of Urea Foliar Nutrition and Leaf Harvesting Intensity on Growth, Yield Attributes and Yield in Cowpea [Vigna unguiculta (L.) Walp.

A field experiment was undertaken at Farming Systems Research Station (FSRS), Sadanandapuram, Kollam district, Kerala Agricultural University in summer 2022 for studying the effect of growth, yield attributes and yield of cowpea through foliar application of 2% urea and intensity of leaf harvesting. Experimental treatments comprised of 4 levels of foliar nutrition management (2% urea spray at vegetative stage (f1), 2% urea spray at flowering stage (f2), 2% urea spray at vegetative stage and flowering stage (f3), water spray control (f4) and 3 levels of leaf harvest (20% leaf harvest (l1), 40% leaf harvest (l2), and no leaf harvest (l3). Among the foliar nutrition management treatments, f3 showed significantly the taller plants (91.97 cm), more number of branches (15.13), leaves (34.76), leaf area/ plant (884.29 cm2), leaf area index (1.96) dry matter production (42.39 g) and chlorophyll content (3.544 mg/g) and were comparable with f1. The nodule parameters such as nodule number (37.44) and effective number of nodules (32.11) were the highest in treatment f3 and was on par with f1. Further, days to 50 per cent flowering (53.65 days), pod length (17.27 cm), 100 seed weight (12.40 g), pod number (24.58), foliage yield (1484.57 kg ha-1), grain yield (925.85 kg ha-1) and haulm yield (2722.70 kg ha-1) were the highest in treatment f3 and was on par with f1 except for pod weight (21.19 g). Among the leaf harvest intensity, treatment l3 recorded the taller plants (92.45 cm), leaf area per plant (902.71cm2) leaf area index (2.01), less days to 50% flowering (47.60 days) and haulm yield (2754.85 kg ha-1). It was on par with l1 pertaining to plant height. The number of branches (15.03), dry matter production per plant (40.78g), pod weight/plant (19.43 g), pod number/plant (23.59) and grain yield (925.85 kg ha-1) were the highest in treatment l1.

Detection of phytoconstituents present in Spirulina platensis extract by GC-MS analysis and their formulation activities against Fusarium oxysporum f.sp. vanillae

Abstract Spirulina platensis represents promising sources of bioactive compounds for pharmaceutical and industrial applications. This study aimed to determine the active compounds in S. platensis extracts (SpE) formulation with three different solvents and in vitro testing against Fusarium oxysporum f.sp.vaniliae. This study was conducted from January to July 2023. Identification of the active compounds present in the three SpE formulations was carried out using the GC-MS method. The effectiveness of SpE formulations was carried out with an inhibition test on F. oxysporum using a completely randomized design (CRD). Based on the results of GC-MS analysis, it is known that the main compounds present in the SpE formulation with ethanol solvent (F1) are trilinolein (48.35%), nonadecatriene (12.50%) and cis-9-hexadecenal (6.47%). SpE formulations using ethyl acetate solvent (F2) are cis-9-hexadecenal (19.53%) and benzene dicarboxylic acid (10.73%). SpE formulations using hexane solvent (F3) are oxacycloheptadec (24.83%) and octadecanoic acid (10.41%). Based on the results of Particle Size Analysis (PSA), it is known that the F1 formulation measures 59.22 µm, the F2 (7.22 µm), and the F3 (7.84 µm). The effectiveness testing of the three formulations showed that the SpE formulation with different solvents effectively inhibited the growth of the fungus F. oxysporum with an average inhibition of 8.41% - 100% compared to the control. F2 treatment with 10% and 15% concentrations resulted in the highest inhibition effect, namely 100%. The 10% and 15% concentrations in the F1 treatment were 8.41% and 39.67%, respectively. The F3 treatment produced 0% inhibition because the growth of the fungus F. oxysporum was better than that of the control. This study implies that SpE formulation with ethanol and ethyl acetate solvents has the potential as an antifungal, especially for F. oxysporum. From this study, the active compounds that act as antifungals are cis-9-hexadecenal, benzene dicarboxylic acid and diisooctyl phthalate.

Aktivitas Antioksidan dan Evaluasi Sensori Kukis Tersubstitusi Tepung Kacang Hijau dan Tepung Kulit Buah Naga Merah

Bad eating habits with high fat content but low fiber have been linked to the rise of degenerative diseases, and this is a main driven factor of developing high fiber and antioxidant foods. Green bean flour contains high protein and phenolic compounds such as flavonoids while red dragon fruit peel flour contains high antioxidants and fiber. The aim of this study was to investigate physico-chemical and sensory properties of cookies substituted with green bean and red dragon fruit peel flour. The flour formulations consisted of wheat flour (TT), red dragon fruit peel flour (TN), and green bean flour (TK) as follows: K1 (37.5% TT:62.5% TN), K2­ (37.5% TT:62.5% TK), F1 (38% TT:47% TN:15% TK), F2­ (38% TT:31% TN:31% TK), and F3­ (38% TT:15% TN:47% TK) as well as control K0 (100% TT). Cookies quality was observed, including physical properties, chemical properties, and sensory properties. The results of this study showed that substitution of green bean and red dragon fruit peel flour in the cookies had significant effects on physico-chemical properties and sensory tests. Treatment F3 was the best formulation based on physical, chemical, and sensory properties with hardness (8.75 N), L* (42.28), a* (17.31), b* (22.05), volume development (29.41%), moisture content (5.68%), ash (3.56%), fat (24.47%), protein (9.47%), carbohydrate (57.38%), crude fiber (13.12%), antioxidant capacity of (49.32%), and IC50 (97.92 ppm). The results of the sensory test for F3 sample had scores for color (1.13), aroma (2.43), taste (4.27), texture (3.23), and overall liking (4.30).

Increasing Hybrid Rice Yield, Water Productivity, and Nitrogen Use Efficiency: Optimization Strategies for Irrigation and Fertilizer Management.

Water and fertilizer are crucial in rice growth, with irrigation and fertilizer management exhibiting synergies. In a two-year field study conducted in Yiyang City, Hunan Province, we examined the impact of three irrigation strategies-wet-shallow irrigation (W1), flooding irrigation (W2), and the "thin, shallow, wet, dry irrigation" method (W3)-in combination with distinct fertilizer treatments (labeled F1, F2, F3, and F4, with nitrogen application rates of 0, 180, 225, and 270 kg ha-1, respectively) on rice yield generation and water-fertilizer utilization patterns. The study employed Hybrid Rice Xin Xiang Liang you 1751 (XXLY1751) and Yue Liang you Mei Xiang Xin Zhan (YLYMXXZ) as representative rice cultivars. Key findings from the research include water, fertilizer, variety, and year treatments, which all significantly influenced the yield components of rice. Compared to W2, W1 in 2022 reduced the amount of irrigation water by 35.2%, resulting in a 42.0~42.8% increase in irrigation water productivity and a 25.7~25.9% increase in total water productivity. In 2023, similar improvements were seen. Specifically, compared with other treatments, the W1F3 treatment increased nitrogen uptake and harvest index by 1.4-7.7% and 5.9-7.7%, respectively. Phosphorus and potassium uptake also improved. The W1 treatment enhanced the uptake, accumulation, and translocation of nitrogen, phosphorus, and potassium nutrients throughout the rice growth cycle, increasing nutrient levels in the grains. When paired with the F3 fertilization approach, W1 treatment boosted yields and improved nutrient use efficiency. Consequently, combining W1 and F3 treatment emerged as this study's optimal water-fertilizer management approach. By harnessing the combined effects of water and fertilizer management, we can ensure efficient resource utilization and maximize the productive potential of rice.

Effects of N, P, and K application rates on distribution of 13C assimilates, starch accumulation in grains and fertilizer utilization of wheat

Clarifying the appropriate application rates of N, P, and K fertilizers and the physiological mechanisms of wheat under water-saving recharge irrigation in the North China Plain would provide a theoretical basis for formulating reasonable fertilization plans for high-yield and high-efficiency wheat production. We established four treatments with different amounts of nitrogen (N), phosphorus (P2O5), and potassium (K2O) application: 0, 0, and 0 kg·hm-2 (F0), 180, 75, and 60 kg·hm-2 (F1), 225, 120, and 105 kg·hm-2 (F2), and 270, 165, and 150 kg·hm-2 (F3). During the jointing and anthesis stages of wheat, the relative water content of each treatment in the 0-40 cm soil layer was replenished to 70%, to investigate the differences in wheat flag leaf photosynthetic characteristics, distribution of 13C assimilates, grain starch accumulation, and fertilizer utilization. The results showed that the relative chlorophyll content of flag leaves, photosynthetic and chlorophyll fluorescence parameters, 13C assimilate allocation in each organ, enzyme activities involved in starch synthesis, and starch accumulation in the F1 treatment were significantly higher than that in F0 treatment, which was an important physiological basis for the 20.9% increase in grain yield. The above parameters and yield in the F2 and F3 treatments showed no significant increase compared to F1 treatment, while fertilizer productivity and agronomic efficiency of N, P, and K decreased by 17.5%-58.4% and 12.7%-50.7%, respectively. Therefore, F1 could promote flag leaf photosynthetic assimilate production and grain starch accumulation under water-saving supplementary irrigation conditions, resulting in higher grain yield and fertilizer utilization efficiency.

Response of chickpea's yield and yield components to double inoculation with mycorrhiza and rhizobium under split application of nitrogen

AbstractThe utilization of advantageous microorganisms as a biofertilizer has gained significant importance in the agricultural industry due to their potential contribution to food safety and the sustainable cultivation of crops. To evaluate the effect of nitrogen (N) fertilizer and co‐inoculation with arbuscular mycorrhiza and rhizobium on the yield and yield component of chickpea (Cicer arietinum L.), a 2‐year field experiment was conducted in 2020–2021 at the Agricultural Research Station of Ferdowsi University of Mashhad, Iran. The experiment was designed as a randomized block trial in factorial design with three replicates. The treatments included two levels of inoculation (noninoculated and inoculated with rhizobium and mycorrhiza) and application of different levels of N fertilizer (0, 25, or 50 kg ha−1) at three growth stages (sowing, flowering, pod filling) as follows; F0 (0,0,0), F1 (25,0,0), F2 (25,25,0), F3 (25,25,25), F4 (50,0,0), F5 (50,50,0) and F6 (50,50,50), respectively. The results showed that seed inoculation and split N fertilization significantly increased yield and yield components in chickpea (Cicer arietinum L.). Seed inoculation showed the highest values for all traits studied compared to the noninoculated treatments. Moreover, among the fertilizer treatments, the highest values for plant height (41.8 cm), number of branches (9.1), number of grains per plant (17.8) and 100‐grain weight (30.4 g) from F6 through the F3 treatment, were statistically similar. The results show that the effect of inoculation is more significant when a lower amount of N fertilizer is applied. Due to the health and environmental problems associated with chemical fertilizers, double inoculation and split application of N fertilizers at lower doses can be recommended.

Synergistic effects of rhizosphere effect and combined organic and chemical fertilizers application on soil bacterial diversity and community structure in oilseed rape cultivation.

The combined application of chemical and organic fertilizers has been recognized to enhance soil fertility and foster the soil microbial ecosystem. However, the optimal ratio of chemical and organic fertilizers in oilseed rape cultivation is still uncertain, and the role of rhizosphere effect is still unclear. Thus, this study aimed to elucidate the impacts of varying ratios of chemical and organic fertilizers on the structure and potential functionalities of rhizosphere and non-rhizosphere soil microbial communities. The interplay of microbial communities with soil properties and oilseed rape root exudates was investigated in controlled pot cultivations receiving varying ratios of chemical and organic fertilizers. Results indicated clear segregation in the soil bacterial community, influenced by both fertilization treatments and rhizosphere effects. The bacterial community structure significantly correlated with nitrate nitrogen, organic acids, and dissolved organic carbon (DOC) content. Rhizosphere effects led to increased bacteria abundance, reduced diversity, and decreased network stability. Notably, F3 treatment receiving 25% chemical and 75% organic fertilizers showed a significantly higher abundance at 1.43 × 1011 copies g-1 dry soil, accompanied by increased species and genetic diversity, and ecological network complexity. This treatment also yielded the highest aboveground biomass of oilseed rape. However, the application of organic fertilizers also increased the risk of plant pathogenicity. This study reveals the impact of fertilizers and rhizosphere effects on soil microbial community structure and function, shedding light on the establishment of more effective fertilization schemes for oilseed rape agriculture.

Assessment of the yield and yield attributing characters of oat (Avena sativa L.) grown under different tillage methods and NPK fertilizer rates in semi-arid conditions

Field experiments were conducted to determine the effect of tillage practices and inorganic sources of different combinations of nitrogen, phosphorus, and potassium fertilizers on some physical properties of soil and the yield of oats. The main plots were tillage techniques of no-till (T1), tillage with a cultivator (T2), twice-disc harrow (T3), and moldboard plowing (T4). Split-plots were fertilizer treatments of F1 control without fertilizer, 70:45:50 kg ha−1 NPK (F2), 140:90:100 kg ha−1 NPK (F3), 210:135:150 kg ha−1 NPK (F4), and 280:180:200 kg ha−1 NPK (F5). Soil density, acidity, electrical conductivity, and soil organic carbon increased under T1, while saturated hydraulic conductivity decreased compared with other tillage practices. Soil bulk density and pH increased with increasing sampling depth, while electrical conductivity and soil organic carbon declined with depth. Results from this study indicated a reduction in saturated hydraulic conductivity with time. The highest grain yield was found in the T4 and T3. In contrast, the highest grain yield was observed under F4 and F5 fertilization treatments at location 1 and F5 and F4 treatments at location 2. There were significant differences in growth characteristics and yield components due to the influence of tillage practices and fertilization levels in both experiment locations.

Antifungal effectiveness of some essential oils and their mixtures against Fusarium oxysporum f.sp cubense that causes fusarium wilt disease of banana plants

Antifungal effectiveness of several essential oil botanical pesticides and their mixtures against the fungus Fusarium oxysporum f.sp. cubense (Foc) which causes fusarium wilt disease of banana plants in vitro, was conducted from March to July 2023. The aim of the study was to obtain an effective botanical pesticide formulation and the right concentration for controlling the Foc causing Fusarium wilt disease in banana plants. The study was arranged in a completely randomized design in factorials with three replications. The experiment was carried out using two methods: a) Suppressing colony diameter using Potato Dextrose Agar (PDA) medium, b). Suppressing colony biomass using Potato Dextrose Broth (PDB) medium. The treatment was the formulation of essential oil (F) botanical pesticides as the first factor; F1 (Cymbopogon flexuosus oils), F2 (C. nardus essential oils), F3 (Eugenia aromatica leaf essential oils), F4 (Cinnamomum burmanii leaf oils), F5 (F1+F2), F6 (F1+F3), F7 (F1+F4) and F8 (F2 +F3), F9 (F2+F4) and F10 (F3+F4) The level of concentration (C) as a second factor C1(1000 ppm) and C2(2000 ppm). The results showed that the F3 (E. aromatica leaf oils) treatment had the best antifungal activity against Foc. At a concentration of 2000 ppm inhibition of the diameter growth and colony biomass reached 100%, followed by the F1 treatment (C. flexuosus oils) up to 89.73% and 95.75%, the F2 treatment (C. nardus oils) reach 84.58% and 89.67% and the lowest antifungal activity is F4 (C. burmanii leaf oils) which only 77.08% and 84.50%. The best mixed formulations were F5(F1+ F3) and F8(F2 + F3), at the same concentration suppression of colony growth reaching 100%. In general, mixed essential oils formulation have shown to increase the effectiveness of the antifungal activities.

PENGARUH PENAMBAHAN BUAH BIT (Beta vulgaris L.) DAN BUBUK SARI KACANG MERAH (Phaseolus vulgaris L.) PADA PEMBUATAN BOBA

Introduction: Boba is a drink topping. The target market is teenagers. Teenagers are one of the groups prone to anemia. Anemia is prevented by eating foods containing iron, for example beets and red beans. This research aims to analyze the quality, acceptability and nutritional characteristics. Method: Experimental study design with a 2-factor, Completely Randomized Design with 3 treatment levels. The study was conducted with 40 untrained panelists. Statistical data analysis Friedman test and Wilcoxon test. Result: Sensory, hedonic and ash content analysis results indicated that the F3 treatment was superior. Water content test results received more F1 treatment. The results of the iron test showed that the F2 treatment was better. Conclusion: There are significant differences based on sensory analysis research results. The highest moisture content test results were obtained in F1, the highest ash content test in F3 and the highest iron content test in F2.

History of exposure to copper influences transgenerational gene expression responses in Daphnia magna

ABSTRACT The establishment of transgenerational effects following chemical exposure is a powerful phenomenon, capable of modulating ecosystem health beyond exposure periods. This study assessed the transgenerational effects occurring due to copper exposure in the invertebrate D. magna at the transcriptional level, while evaluating the role of exposure history on such responses. Thus, daphnids acclimated for several generations in a copper vs. clean medium were then exposed for one generation (F0) to this metal, and monitored for the following non-exposed generations (F1, F2 and F3). Organisms differing in exposure histories showed remarkably different transcriptional profiles at the F0, with naïve organisms being more profoundly affected. These trends were confirmed for F3 treatments, which presented different transcriptional patterns for genes involved in detoxification, oxidative stress, DNA damage repair, circadian clock functioning and epigenetic regulation. Furthermore, regardless of exposure history, a great number of histone modifier genes were always found transcriptionally altered, thus suggesting the involvement of histone modifications in the response of Daphnia to metal exposure. Lastly, remarkably distinct transgenerational transcriptional responses were found between naïve and non-naïve organisms, thereby highlighting the influence of exposure history on gene expression and confirming the capacity of metals to determine transgenerational transcriptional effects across non-exposed generations.

Substitution of Noodles Made from Banana Flour and Cassava Leaf Flour as Functional Food

Noodles are a processed food rich in starch made from flour moulded lengthwise like threads and are one of the favourite foods of Indonesian people. This activity aims to find out the noodle formulation by substituting banana flour and cassava leaf flour and the results of organoleptic tests and proximate analysis. The research method was carried out experimentally using a Completely Randomized Design (RAL). The Kruskall Wallis test results, colour, aroma, texture and taste parameters showed P<0.05, there was a significant difference in the treatment of F1, F2, F3, and F4 with the substitution of banana flour and cassava leaf flour. The ANOVA test results showed P<0.05, there were significant differences in the F1, F2, F3, and F4 treatments on water content, ash content, protein content, fat content, crude fibre content and carbohydrate content in noodles with the substitution of banana flour and leaf flour. cassava. This activity concludes that there is a real difference in the treatment of F1, F2, F3, and F4 with the substitution of banana flour and cassava leaf flour. There were real differences in the treatment of F1, F2, F3, and F4 on water content, ash content, protein content, fat content, crude fibre content and carbohydrate content in noodles with the substitution of banana flour and cassava leaf flour. The suggestion from this research is that banana flour and cassava leaf flour have the potential to substitute ingredients for wheat in making functional foods.

Effect of Bio-NPK and Organic Manures on Growth, Yield and Economics of Cauliflower (Brassica oleracea var. botrytis l.) var. Pusa Snowball K 1

The present investigation was carried out on “Effect of Bio-NPK and organic manures on growth, yield and economics of cauliflower (Brassica oleracea var. botrytis l.) var. Pusa Snowball K 1” at Horticulture Research Farm, Department of Horticulture, B. A. College of Agriculture, Anand Agricultural University, Anand during Rabi season 2022-23. The experiment was laid out in Randomized Block Design with factorial concept with three replication and thirteen treatment combinations comprising of two level of Bio-NPK and six level of organic manures of total 12 combinations. The experiment result revealed that, treatment B1 (seedling dipping with Bio-NPK) was found the most effective treatment which recorded significantly maximum plant height (44.97, 51.74 and 58.16 cm), number of leaves at (10.65, 16.84 and 23.42), N-S plant spread (37.04, 50.67 and 63.88 cm) and E-W plant spread (34.78, 48.79 and 62.26 cm) at 40th, 60th and 80th DAT, respectively, weight of the curd (745.16 gm), diameter of the curd (16.99 cm) and yield per plot (13.46 kg). Treatment of organic manures significantly responded on growth and yield parameters viz., Among all treatment, treatment F3 (100 % RDN through VC) was the most effective treatment which recorded significantly maximum plant height (46.84, 54.42 and 61.00 cm), number of leaves (11.26, 17.66 and 24.19), plant spread N-S (37.58, 52.26 and 65.86), E-W (35.40, 50.05 and 64.25 cm) at 40th, 60th and 80th DAT respectively, weight of the curd (802.41 gm), diameter of the curd (17.69 cm) and yield per plot (14.72 kg). However, based on economics, treatments 75 % RDN through VC + 25 % RDN through FYM gave maximum net realization (197168 ₹ /ha).