F1 Group Research Articles

The effect of crossbreeding with different breeds on slaughter and carcass characteristics and meat quality in Leghorn hens

This study reports slaughter and carcass characteristics and meat quality as a result of crossing Leghorn chickens and five different genotypes. The experiment was carried out in the Prof. Dr. Hümeyra Özgen Research and Application Farm in Selcuk University. The material consisted of 20 leghorn crosses (F2) of each genotype reared under the same feeding and care conditions. The animals were slaughtered at 84 d. In this study, the highest value was determined in the Brahma × Leghorn (BL) genotype, followed by the Cornish × Leghorn (CL), Denizli × Leghorn (DL), and Aracuana × Leghorn (AL) genotypes, respectively, according to slaughter weight and hot and cold carcass performance values. The lowest value was calculated in the Leghorn × Leghorn (LL) genotype, as expected. Slaughter, cold, and carcass weights were found to be similar. A difference was found in colour characteristics (L*,a*,b*) of thigh (b), breast (l), and breast (a) meats of F2 groups. A difference was found in the DM and pH values of the genotypes. CL came into prominence when rearing specific genotypes as free-range broilers. In evaluating the parts of the carcass, it was found that two genotypes were superior to other genotypes in terms of thigh, breast, wing, and back weights. New three-unit and four-unit crossbreeding studies have a potential to add new local commercial genotypes to national economies.

Restraint stress-induced effects on learning, memory, cognition, and expression of transcripts in different brain regions of mice.

Stress is one of the prevalent factors influencing cognition. Several studies examined the effect of mild or chronic stress on cognition. However, most of these studies are limited to a few behavioral tests or the expression of selected RNA/proteins markers in a selected brain region. This study examined the effect of restraint stress on learning, memory, cognition, and expression of transcripts in key learning centers. Male mice were divided into three groups (n = 6/group)-control group, stress group (adult stressed group; S), and F1 group (parental stressed group). Stress group mice were subjected to physical restraint stress for 2h before light offset for 2weeks. The F1 group comprised adult male mice born of stressed parents. All animals were subjected to different tests and were sacrificed at the end. Transcription levels of Brain-Derived Neurotrophic Factor (Bdnf), Tyrosine kinase (TrkB), Growth Associated Protein 43 (Gap-43), Neurogranin (Ng), cAMP Response Element-Binding Protein (Creb), Glycogen synthase kinase-3β (Gsk3β), Interleukine-1 (IL-1) and Tumour necrosis factor-α (Tnf-α) were studied. Results show that both adult and parental stress negatively affect learning, memory and cognition, as reflected by taking longer time to achieve the task or showing reduced exploratory behavior. Expression of Bdnf, TrkB, Gsk3β and Ng was downregulated, while IL-1 and Tnf-α were upregulated in the brain's cortex, thalamus, and hippocampus region of stressed mice. These effects seem to be relatively less severe in the offspring of stressed parents. The findings suggest that physical restraint stress can alter learning, memory, cognition, and expression of transcripts in key learning centers of brain.

Effect of filler contents on the bond strength of CAD/CAM resin crowns: New resin primer versus conventional silane agents.

Purpose This study aims to evaluate the effects of resin primers containing methyl methacrylate (MMA) and silane agent on the bonding effectiveness of indirect resin composite blocks with three different filler contents.Methods A commercially available computer-aided design/computer-aided manufacturing (CAD/CAM) resin composite block and two experimental resin composite blocks with different filler contents were alumina-blasted and two surface treatments (primer and silane agent) were applied. The resin cement was built up, and the micro-tensile bond strength (μTBS) was measured after 24 hours, 1 month or 3 months of water storage (n = 24 per group). The fracture surfaces after μTBS measurements and resin block/cement interface were observed by scanning electron microscopy (SEM).Results The primer treatment group showed a significantly higher bond strength than the silane group only in F0 (filler content 0 wt%) group (P < 0.001). In the primer group, F0 and F41 (filler content 41 wt%) groups showed significantly higher bond strengths than F82 (filler content 82 wt%) group (P < 0.001). In contrast, in the silane group, F41 group showed significantly higher bond strength than F0 and F82 groups (P < 0.001), and F82 group showed significantly higher bond strength than F0 group (P < 0.001). SEM revealed that the matrix resin was partially destroyed on the fracture surface of the primer group, and an uneven interface surface was observed compared with that of the silane group.Conclusions MMA-containing primers showed higher bonding effectiveness to CAD/CAM resin composite blocks than the silane treatment.

Reproductive and whole-body toxicity of Ag-doped and -undoped ZIF-8 nanoparticles and the building blocks: An Artemia-based comparative bioassay

The present research assessed, for the first time, toxicity of ZIF-8 (1 mg/L) and the building blocks (0.1 mg/L Zn2+ and 0.4 mg/L 2-methylimidazole (2-MIm)), besides that of AgNPs@ZIF-8 (0.25, 0.5, and 1 mg/L) and AgNO3 (0.1 mg/L) to aquatic organisms. Two consecutive generations (F0 & F1) of Artemia salina were exposed to these chemicals. All of the chemical treatments considerably caused mortality in F0, especially AgNPs@ZIF-8 and AgNO3, whereas F1 displayed notable tolerance and survived comparable to the control group, except in the case of AgNO3 treatment. Similarly, growth indices (weight, mainly in ZIF-8, Zn2+, and 2-MIm; length, in Ag-doped ZIF-8 and AgNO3) were significantly retarded in F0 and especially F1 of all treatments, and 2-MIm caused the greatest length retardation in F0. AgNPs@ZIF-8 (0.5 and 1 mg/L), 2-MIm, and AgNO3 postponed the ovary emergence in about 40%–60% of the exposed F0, and ZIF-8 delayed this phenomenon in some individuals of F0 and F1 up to 6 days. This temporal disturbance was also observed in time to first brood of almost all experimental F0 and F1 groups, with being over 80% of F1 exposed to ZIF-8, 2-MIm, and Zn2+, as well as about 50% of F0 treated with 2-MIm, and Zn2+. The highest neonate number was recorded for F0 and F1 exposed to AgNO3 and Zn2+, while ZIF-8 and, importantly, 2-MIm decreased the reproductivity to the lowest levels in both generations. Generally, the reproductive frequency was significantly decreased in all F0 and F1 treatments, especially 2-MIm, ZIF-8, AgNPs@ZIF-8 (0.25 & 1 mg/L). This study highlighted the neglected importance of 2-MIm in assessing overall toxicity of ZIF-8, and even other organic ligands of MOFs, and also filled a gap in the literature by investigating the potential effect of additives such as AgNPs on the toxicity of ZIF-8 and other MOFs.

Simple algebraic groups with the same maximal tori, weakly commensurable Zariski-dense subgroups, and good reduction

We provide a new condition for an absolutely almost simple algebraic group to have good reduction with respect to a discrete valuation of the base field which is formulated in terms of the existence of maximal tori with special properties. This characterization, in particular, shows that the Finiteness Conjecture for forms of an absolutely almost simple algebraic group over a finitely generated field that have good reduction at a divisorial set of places of the field (cf. [66]) would imply the finiteness of the genus of the group at hand. It also leads to a new phenomenon that we refer to as “killing the genus by a purely transcendental extension.” Yet another application deals with the investigation of “eigenvalue rigidity” of Zariski-dense subgroups (cf. [64]), which in turn is related to the analysis of length-commensurable Riemann surfaces and general locally symmetric spaces. Finally, we analyze the Finiteness Conjecture and the genus problem for simple algebraic groups of type F4.

Growth and Development Performance of Hermetia illucens L. (Diptera: Stratiomyidae) Larvae on Fermented Palm Kernel Meal (PKM) Substrate

Larvae of Hermetia illucens (Black Soldier Fly/BSF) are considered agents of bioconversion of organic waste, including by-products of agroindustrial waste. Palm kernel meal (PKM), a palm oil mill waste contains high lignocellulose, making it difficult for BSF larvae to digest in which pre treatment process is required. This study aims to analyze the growth and development performance of BSF larvae in fermented PKM waste using EM4 and molasses as the pretreatment process. Five (5) days old BSF larvae were reared in PKM waste, which was fermented with EM4 and molasses for 2, 3, and 4 days (F2, F3, and F4) and with water for four days (FA) while chicken feed (PA) applied as control. During this study, growth rate, average weight, developmental period, survival rate, and larval development period. Feed efficiency and feed reduction analyzed by efficiency of conversion of digested food (ECD), feed conversion ratio (FCR), waste reduction index (WRI) and substrate consumption rate (SCR). The growth and development performance of F2 group was the best among other treatment groups which is similar to larvae fed on chicken feed as a control. All treatments showed high larval survival rates (99.72-100.00%). On the other hand, the best best reduction efficiency recorded in F3. Based on these result it can be concluded that pretreatment of PKM by EM4 and molasses is applicable to be improve the quality of PKM as feeding material for BSF larvae.

Impact of Liver Fibrosis Severity on Oncological Prognosis in Hepatocellular Carcinoma

Introduction: Cirrhosis is deemed to be a contributing factor to the postoperative recurrence of hepatocellular carcinoma (HCC); however, the precise impact of liver fibrosis on both cancer-specific prognoses remains unclear. This investigation sought to elucidate the effect of liver fibrosis severity on the cancer-specific prognosis. Methods: A total of 524 consecutive patients were included. Recurrence-free survival (RFS) and disease-specific survival (DSS) were compared according to fibrosis stage. Moreover, postoperative outcomes were subjected to analysis in cohorts of patients with F0 and F1–3, as well as in those with F1–3 and F4, who were carefully matched for background factors. Results: The 5-year RFS exhibited a significantly worse outcome in the F4 group compared to other stages of fibrosis: 5-year RFS – F0 (46.6%), F1–3 (33.1%), and F4 (23.5%), p = 0.03 (F0 vs. F1–3) and p < 0.01 (F1–3 vs. F4). Additionally, the 5-year DSS also presented a significantly worse prognosis in the F4 group: 5-year DSS – F0 (82.9%), F1–3 (73.6%), and F4 (57.4%), p = 0.04 (F0 vs. F1–3) and p < 0.01 (F1–3 vs. F4). In multivariate analysis, fibrosis 1, 2, 3, and 4 stage (compared with F0) (HR: 1.70, 1.81, 1.89, and 3.99, 95% confidence interval: 1.10–1.99, 1.39–2.22, 1.41–2.55, and 2.25–5.01, p = 0.022, p = 0.008, p < 0.001, and p < 0.001, respectively) was independent risk factor for RFS. After matched analysis, both RFS and DSS exhibited significantly worse prognoses in the presence of more advanced fibrosis. There was a significantly higher incidence of multiple recurrences in the F4 group than the F1–3 group, and a number of recurrences were observed both in the same hepatic segment as the resected side and in the contralateral lobe in F4 group. Discussion/Conclusion: The hazard and recurrence pattern of HCC signifies that the prognosis could potentially be poor, as the hepatic fibrosis likely owing to a higher hepatocarcinogenic potential, even in the absence of progression to cirrhotic condition. The risk of de novo recurrence may also increase with the progression of this fibrosis.

An Assessment of Pb and Cu in Waters, Sediments, and Mud Crabs (Scylla serrata) from Mangrove Ecosystem Near Tanjung Api-Api Port Area, South Sumatra, Indonesia

Heavy metal pollution from anthropogenic activities can harm aquatic ecosystems. This study aims to determine the concentration of heavy metals (Pb and Cu) in waters, sediments, and mud crabs (Scylla serrata), and to analyze the relationship between environmental parameters and S. serrata consumed by humans. Samples were taken in the mangrove ecosystem around the Tanjung Api-Api port area in South Sumatra, Indonesia. Pb and Cu analysis used the Atomic Absorption Spectrophotometer (AAS). Pb and Cu linkages in waters, sediments, and S. serrata analyzed by SigmaPlot V12.5 and Principal Component Analysis (PCA) analyzed by XLSTAT 2022. The limit consumption of S. serrata was calculated using MWI (Maximum Weekly Intake) and MIT (Maximum Intake Tolerance). Based on the results, the heavy metal Pb in water was 0.1055 – 0.1322 mg.L-1, and Cu was not detected. Furthermore, Pb in sediments ranged from 7.0104 - 11.8186 mg.kg-1, Cu 3.7127 - 4.5347 mg.kg-1, and Pb in S. serrata ranged from 0.0001 - 0.0021 mg.kg-1, and Cu ranged from 0.03 – 0.0791 mg.kg-1. The concentration of heavy metals in water, sediment, and S. serrata had not exceeded the specified quality standard, except for Pb in water. The principal component analysis obtained F1 (44.35%), F2 (27.53%) and F3 (17.83%) groups. Based on MWI and MIT value, it showed high quality which concluded S. serrata was still safe for human consumption.

Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into six groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), F3 (treatment with 80% areca nut:20% chrysanthemum), and normal group (without ulcer and treatment). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2, F3, and the positive control group (Dependent t-test, p&lt;0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p&lt;0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test, p&lt;0.05). In the collagenases assay, a high concentration of areca nut (F3) induced the abundance of collagen during the ulcer healing process. Conclusions: The combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in Sprague-Dawley rats through the increase of TNF-α and collagen deposition.

A randomized trial comparing beam F3 and 5.5 cm targeting in rTMS treatment of depression demonstrates similar effectiveness

BackgroundThe Beam F3 and 5.5 cm methods are the two most common targeting strategies for localizing the left dorsolateral prefrontal cortex (DLPFC) treatment site in repetitive transcranial magnetic stimulation (rTMS) protocols. This prospective, randomized, double-blind comparative effectiveness trial assesses the clinical outcomes for these two methods in a naturalistic sample of patients with major depressive disorder (MDD) undergoing clinical rTMS treatment. Methods105 adult patients with MDD (mean age = 43.2; range = 18–73; 66% female) were randomized to receive rTMS to the Beam F3 (n = 58) or 5.5 cm (n = 47) target. Between group differences from pre-to post-treatment were evaluated with the Patient Health Questionnaire-9 (PHQ-9) [primary outcome measure], Generalized Anxiety Disorder-7 (GAD-7), and clinician-administered Montgomery-Åsberg Depression Scale (MADRS). Primary treatment endpoint was completion of daily treatment series. ResultsPer-protocol analyses showed no statistically significant differences on any measure between the 5.5 cm and F3 groups (all p ≥ 0.50), including percent improvement (PHQ-9: 39% vs. 39%; GAD-7: 34% vs. 27%; MADRS: 40% vs. 38%), response rate (PHQ-9: 37% vs. 43%; GAD-7: 27% vs. 30%; MADRS: 43% vs. 43%), and remission rate (PHQ-9: 22% vs. 21%; MADRS: 20% vs. 19%). Post hoc analysis of anxiety symptom change while controlling for depression severity suggested more favorable anxiolytic effects with 5.5 cm targeting (p = 0.03). ConclusionsSimilar antidepressant effects were observed with DLFPC rTMS using either the Beam F3 or 5.5 cm targeting method, supporting clinical equipoise in MDD patients with head circumference ≤ 60 cm. Comparison to MRI-based targeting and differential effects on anxiety symptoms require further investigation. Trial registrationClinicalTrials.gov identifier: NCT03378570.

The Effect of Donors Used in In vivo Maternal Haploid Technique on Haploid Induction Rate

Maize is an important plant grown to obtain grain and silage, and is used in human and animal nutrition. In conventional maize breeding studies, inbred line development studies are carried out for at least 7 years if a single generation is obtained in a year, while it is possible to develop 100% homozygous lines in a short period of 2 years with the in vivo maternal haploid technique. The in vivo maternal haploid technique is widely used in advanced maize breeding programs. The choice of donor or source material to be used for haploid induction depends on the purpose of the breeding program. Generally, breeders use F1 or F2 populations as source material for haploid induction. In this study; 30 F1 genotypes and their F2s were crossed with the inducer line. The putative haploid seed was identified by considering the R1-nj color marker, and the haploid induction rate was determined. The effect of the generations of the donor genotypes on the haploid induction rate was compared by performing an independent sample test, and the haploid induction rate obtained from the F1 donors was found to be higher than the haploid induction rate of the F2 donors. It was determined that there was a change in the haploid induction rate as the genotypes changed within the F1 and F2 donor groups.

Prediction of Early-stage Liver Fibrosis Using FDG-PET/CT.

18F-fluorodeoxyglucose (FDG) is known to accumulate in the liver. We investigated whether accumulation of FDG was correlated with the degree of liver fibrosis and the grade of necro-inflammatory activity. This retrospective study included 35 patients who underwent FDG-positron emission tomography (PET)/computed tomography (CT) before liver surgery. On fusion images of CT and PET, by placing regions of interest on the lateral, anterior and posterior segments of the liver and the aorta, the standardized uptake value (SUV) mean, and SUV normalized by lean body mass (SUL) mean of the liver were measured, and the ratio SUVmean liver/SUVmean aorta was calculated. According to the New Inuyama Classification, subjects were classified into three groups based on the grade of liver-fibrosis degree, i.e., F0, F1+F2 and F3+F4, and into three groups based on the grade of necro-inflammatory activity, i.e., A0, A1 and A2. Each of the above parameters was then compared among the groups using a Tukey test. Average SULmean liver values of the F0, F1+F2 and F3+F4 groups were 1.573±0.211, 1.845±0.220 and 1.716±0.119, respectively. The SULmean liver of the F1+F2 group was significantly higher than that of the F0 group (p=0.0296). No significant difference was observed for the other two parameters. None of the parameters exhibited significant difference among the A0, A1, and A2 groups. FDG accumulation in the liver may be increased in the early stage of liver fibrosis. SULmean liver could be used to determine the necessity for therapeutic intervention in chronic liver disease.

On construction of maximal parabolic centralizers of root elements and the Weyl group of type F4 in E6(K) for fields K of characteristic 2

The purpose of this paper is to give an elementary and explicit construction of maximal parabolic centralizers of root elements in the Chevalley group E6(K) and to show that the centralizer of a Seigel involution in the Weyl group W of type E6(K) is the Weyl group of type F4, or equivalently it is the stabilizer of a totaly singular line L in W, using properties of the generalized quadrangle (Ω,L) of type O6−(2).The construction here of the maximal parabolic centralizers of root elements in E6K for fields K of characteristic 2, is somewhat novel, beginning as it does, with root bases of Levi-type and root bases of unipotent type, giving an explicit construction of the group generated by these two types.

Ointment Formulation of Tapak Dara (Catharanthus roseus (L.) G. Don) Flower Ethanol Extract and its Activity in Burn-Healing

Treatment done on burn wounds is intended to provide local therapy to heal as quickly as possible. The content of secondary metabolites in the tapak dara (Catharanthus roseus (L.) G. Don) flower can help the healing process of burns, namely alkaloids, saponins, tannins, and flavonoids. Alkaloids act as antibacterial; saponins can trigger collagen formation; tannins as astringents that cause shrinkage of skin pores and stop minor bleeding in wounds; and flavonoids have anti-inflammatory effects. This study aimed to formulate an ointment of C. roseus flower ethanol extract and determine its physical characteristics such as organoleptic test, homogeneity, pH value, dispersion, and stability test of the preparation and examine the activity as a burn healer in white male rats. The research data were analyzed statistically using the ANOVA method, followed by the LSD test (least significant difference) to see how the ointment-containing extract reduced the diameter and percentage of the burn wounds. The results show that all ethanol extracts of C. roseus flower ointments met the requirements for its physical characteristic tests. It offers a good activity as a burn healer in white male rats. The most effective concentration is an ointment containing 15% of ethanol extract from C. roseus flower (F3 group), which shows a significant difference (p &lt;0.05) from the blank and the other group formula in burn wound healing.

Predictors of severe submucosal fibrosis during endoscopic submucosal dissection in patients with ulcerative colitis: Retrospective cohort study.

Severe submucosal fibrosis is a crucial technical difficulty encountered during endoscopic submucosal dissection (ESD) in patients with ulcerative colitis (UC). We aimed to identify predictors of severe submucosal fibrosis in patients with UC. We retrospectively included 55 tumors resected using ESD from 48 consecutive patients with UC. We analyzed the clinicopathological characteristics and treatment outcomes between the F0/1 (none to mild submucosal fibrosis) group (n = 28) and F2 (severe submucosal fibrosis) group (n = 27). No significant difference was found between the F0/1 and F2 groups in en bloc resection rate (100% vs. 96%, P = 0.49), the R0 resection rate (100% vs. 93%, P = 0.24), and the dissection speed (0.18 vs. 0.13 cm2 /min, P = 0.07). Intraoperative perforation was more common in the F2 group (30%) than in the F0/1 group (8%; P = 0.01). Multivariable analysis showed that a longer duration of UC (≥10 years; odds ratio [OR] 6.11; 95% confidence interval [CI] 1.20-31.03; P = 0.03) and scarring of background mucosa of the tumor (OR 39.61; 95% CI 3.91-400.78; P < 0.01) were independent predictors of severe submucosal fibrosis. Long UC duration and scarring background mucosa were predictors of severe submucosal fibrosis associated with perforation during ESD.

Vowel classification with combining pitch detection and one‐dimensional convolutional neural network based classifier for gender identification

AbstractHuman speech signals may contain specific information regarding a speaker's characteristics, and these signals can be very useful in applications involving interactive voice response (IVR) and automatic speech recognition (ASR). For IVR and ASR applications, speaker classification into different ages and gender groups can be applied in human–machine interaction or computer‐based interaction systems for customised advertisement, translation (text generation), machine dialog systems, or self‐service applications. Hence, an IVR‐based system dictates that ASR should function through users' voices (specific voice‐frequency bands) to identify customers' age and gender and interact with a host system. In the present study, we intended to combine a pitch detection (PD)‐based extractor and a voice classifier for gender identification. The Yet Another Algorithm for Pitch Tracking (YAAPT)‐based PD method was designed to extract the voice fundamental frequency (F0) from non‐stationary speaker's voice signals, allowing us to achieve gender identification, by distinguishing differences in F0 between adult females and males, and classify voices into adult and children groups. Then, in vowel voice signal classification, a one‐dimensional (1D) convolutional neural network (CNN), consisted of a multi‐round 1D kernel convolutional layer, a 1D pooling process, and a vowel classifier that could preliminary divide feature patterns into three level ranges of F0, including adult and children groups. Consequently, a classifier was used in the classification layer to identify the speakers' gender. The proposed PD‐based extractor and voice classifier could reduce complexity and improve classification efficiency. Acoustic datasets were selected from the Hillenbrand database for experimental tests on 12 vowels classifications, and K‐fold cross‐validations were performed. The experimental results demonstrated that our approach is a very promising method to quantify the proposed classifier's performance in terms of recall (%), precision (%), accuracy (%), and F1 score.

Convex PBW-type Lyndon Bases and Restricted Two-parameter Quantum Group of Type F4

Convex PBW-type Lyndon Bases and Restricted Two-parameter Quantum Group of Type F4

MP05-13 COMPARISON OF EFFICACY AND SAFETY OF NARROW FOCUS VERSUS WIDE FOCUS SHOCK WAVE LITHOTRIPSY IN MANAGEMENT OF 1-2CM RENAL CALCULI USING TRI-FOCAL LITHOTRIPSY MACHINE: FIRST DOUBLE-BLIND RANDOMIZED STUDY

You have accessJournal of UrologyCME1 Apr 2023MP05-13 COMPARISON OF EFFICACY AND SAFETY OF NARROW FOCUS VERSUS WIDE FOCUS SHOCK WAVE LITHOTRIPSY IN MANAGEMENT OF 1-2CM RENAL CALCULI USING TRI-FOCAL LITHOTRIPSY MACHINE: FIRST DOUBLE-BLIND RANDOMIZED STUDY Yasser Noureldin, Walid Abdel Halim, Ezzat Elnahif, Ahmed Abdelbaky, and Salah El Hamshary Yasser NoureldinYasser Noureldin More articles by this author , Walid Abdel HalimWalid Abdel Halim More articles by this author , Ezzat ElnahifEzzat Elnahif More articles by this author , Ahmed AbdelbakyAhmed Abdelbaky More articles by this author , and Salah El HamsharySalah El Hamshary More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003216.13AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The objectives of this study were to compare efficacy and safety of shock wave lithotripsy (SWL) using a narrow focus of 2 mm (F1) versus a wide focus of 8 mm (F3) for renal stones 1-2 cm using a new tri-focal SWL machine. METHODS: This double-blind randomized study recruited adult patients presented with solitary radio-opaque renal pelvic stone 1-2 cm. Patients were electronically randomized into two groups; F1-group received narrow focus (2 mm) SWL while F3-group received wide focus (8 mm) SWL using Piezo Lith3000 plus (Richard Wolf, Germany). Ramping protocol was employed under simple analgesia, with maximum shocks of 3000 and maximum sessions of 3, two weeks apart. Baseline patient’s and stone characteristics were collected. The SFS (%) and complications were identified. Primary outcome was overall SFS (%) calculated one month following the 3rd session. Secondary outcomes were presence of complications in terms of pain and hematuria. Two urinary renal injury markers [neutrophil gelatinase-associated lipocalin (NGAL) and Kidney Injury Molecule-1 (KIM-1)] were measured with ELISA before, 2 hours after and 72 hours after the 1st SWL session. RESULTS: A total of 135 patients were recruited; 67 in F1 group and 68 in F3 group. Preoperative parameters were comparable in terms of age (p=0.16), female gender (p=0.52), BMI (p=0.71), stone size (1.2±0.3 vs. 1.1±0.3 mm; p=0.18), and HU (858±2 vs. 844±2; 0.70), NGAL (101.7±32.5 vs. 104.1±38.33 ng/dL; p=0.71), KIM-1 (4.3±0.9 vs. 4.5±1.1 ng/dL; p=0.17). The overall SFS and auxiliary interventions were comparable (92.5% vs. 91.2%; p=0.77) and (23.4% vs. 23.2%; p=0.23), respectively. In terms of complications, patients in F1 group had significantly higher experience of hematuria (Clavien 1) (95.5% vs. 83.8%; p=0.02), and pain (Clavien 1) (65.7% vs. 19.1%; p<0.001). Following the first SWL session, the rise in 2-h NGAL was comparable (109.6±32.7 vs. 112.7±40.6; p= 0.62). However, the rise in the 2-hr KIM-1 was significantly higher in F1 (4.9±1.1 vs. 4.4±1.4; p=0.022). Nevertheless, both markers were significantly improved to almost half of the baseline after 3 days (56.7±14 vs. 59.7±16.2; p=0.26) and (2.1±0.55 vs. 2.1± 0.5; p=0.96), respectively. CONCLUSIONS: Narrow and wide focus were associated with comparable lithotripsy outcomes and re-treatment rates. However, narrow focus was associated with significantly higher pain and hematuria. Both F1 and F3 SWL of Piezo Lith3000 plus were potentially safe in terms of the renal injury. Source of Funding: None © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e48 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yasser Noureldin More articles by this author Walid Abdel Halim More articles by this author Ezzat Elnahif More articles by this author Ahmed Abdelbaky More articles by this author Salah El Hamshary More articles by this author Expand All Advertisement PDF downloadLoading ...

Evaluasi Sediaan Krim Ekstrak Etanol Daun Tapak Dara (Catharanthus roseus L.) Sebagai Tabir Surya dalam Menghambat Pembentukan Eritema

Sunlight is composed of visible and ultraviolet light, but only 10% of the UV can penetrate the Earth's atmosphere. Exposure to UV-B on human skin can result in sunburn and erythema. To avoid skin damage from UV, sunscreens containing oxybenzone and octinoxate compounds are used. However, these compounds can have adverse effects on the skin, so finding a more natural alternative is essential. Phenolic compounds and flavonoids, which are found in plants, are believed to serve as natural sunscreens and protect the skin from erythema caused by UV exposure. The objective of this study was to assess the efficacy of tapak daura leaf extract cream as a natural sunscreen to prevent erythema in mice. The extract was made using the maceration method with 96% ethanol in a 1:4 ratio. Three sunscreen cream formulations were created using 1%, 1.5%, and 3% concentrations of tapak daura leaf extract, respectively. Formulation F0 did not include the extract. The creams were then tested on mouse skin and exposed to UV-B for eight hours. The results demonstrated that tapak daura leaf extract sunscreen cream could protect against UV-B rays by reducing the number of erythema spots in the F3 group from 6.3 to only two.

Festuca pratensis-like Subgenome Reassembly from a "Chromosomal Cocktail" in the Intergeneric Festulolium (Poaceae) Hybrid: A Rare Chromoanagenesis Event in Grasses.

Festuca and Lolium grass species are used for Festulolium hybrid variety production where they display trait complementarities. However, at the genome level, they show antagonisms and a broad scale of rearrangements. A rare case of an unstable hybrid, a donor plant manifesting pronounced variability of its clonal parts, was discovered in the F2 group of 682 plants of Lolium multiflorum × Festuca arundinacea (2n = 6x = 42). Five phenotypically distinct clonal plants were determined to be diploids, having only 14 chromosomes out of the 42 in the donor. GISH defined the diploids as having the basic genome from F. pratensis (2n = 2x = 14), one of the progenitors of F. arundinacea (2n = 6x = 42), with minor components from L. multiflorum and another subgenome, F. glaucescens. The 45S rDNA position on two chromosomes also corresponded to the variant of F. pratensis in the F. arundinacea parent. In the highly unbalanced donor genome, F. pratensis was the least represented, but the most involved in numerous recombinant chromosomes. Specifically, FISH highlighted 45S rDNA-containing clusters involved in the formation of unusual chromosomal associations in the donor plant, suggesting their active role in karyotype realignment. The results of this study show that F. pratensis chromosomes have a particular fundamental drive for restructuring, which prompts the disassembly/reassembly processes. The finding of F. pratensis "escaping" and rebuilding itself from the chaotic "chromosomal cocktail" of the donor plant points to a rare chromoanagenesis event and extends the view of plant genome plasticity.